4tlp - Revision history

OCA at 07:19, 27 September 2023

OCA — Wed, 27 Sep 2023 07:19:31 GMT

←Older revision		Revision as of 07:19, 27 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4tlp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TLP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TLP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tlp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TLP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TLP FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tlp OCA], [https://pdbe.org/4tlp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tlp RCSB], [https://www.ebi.ac.uk/pdbsum/4tlp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tlp ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tlp OCA], [https://pdbe.org/4tlp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tlp RCSB], [https://www.ebi.ac.uk/pdbsum/4tlp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tlp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 10:45, 15 March 2023

OCA — Wed, 15 Mar 2023 10:45:30 GMT

←Older revision		Revision as of 10:45, 15 March 2023
Line 1:		Line 1:

	==Crystal structure of a alanine91 mutant of WCI==		==Crystal structure of a alanine91 mutant of WCI==
-	<StructureSection load='4tlp' size='340' side='right' caption='[[4tlp]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='4tlp' size='340' side='right'caption='[[4tlp]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4tlp]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Dolichos_tetragonolobus Dolichos~~ tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TLP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4TLP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4tlp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TLP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TLP FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4tlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tlp OCA], [~~http~~://pdbe.org/4tlp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4tlp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4tlp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4tlp ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tlp OCA], [https://pdbe.org/4tlp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tlp RCSB], [https://www.ebi.ac.uk/pdbsum/4tlp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tlp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ICW3_PSOTE ICW3_PSOTE]] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.	+	[https://www.uniprot.org/uniprot/ICW3_PSOTE ICW3_PSOTE] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:

	==See Also==		==See Also==
-	*[[Chymotrypsin ~~Inhibitor~~\|Chymotrypsin ~~Inhibitor~~]]	+	*[[Chymotrypsin inhibitor 3D structures\|Chymotrypsin inhibitor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Dolichos tetragonolobus~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Majumder, S~~]]	+	[[Category: Psophocarpus tetragonolobus]]
-	[[Category: ~~Sen, U~~]]	+	[[Category: Majumder S]]
-	[[Category: ~~Chymotrypsin inhibitor]]~~	+	[[Category: Sen U]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Inhibitor]]~~	+
-	~~[[Category: Tryptophan~~]]	+

OCA at 09:39, 13 February 2019

OCA — Wed, 13 Feb 2019 09:39:12 GMT

←Older revision		Revision as of 09:39, 13 February 2019
Line 3:		Line 3:
	<StructureSection load='4tlp' size='340' side='right' caption='[[4tlp]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4tlp' size='340' side='right' caption='[[4tlp]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4tlp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TLP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4tlp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dolichos_tetragonolobus Dolichos tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TLP FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tlp OCA], [http://pdbe.org/4tlp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tlp RCSB], [http://www.ebi.ac.uk/pdbsum/4tlp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tlp ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tlp OCA], [http://pdbe.org/4tlp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tlp RCSB], [http://www.ebi.ac.uk/pdbsum/4tlp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tlp ProSAT]</span></td></tr>
	</table>		</table>
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Dolichos tetragonolobus]]
	[[Category: Majumder, S]]		[[Category: Majumder, S]]
	[[Category: Sen, U]]		[[Category: Sen, U]]

OCA at 15:51, 5 August 2016

OCA — Fri, 05 Aug 2016 15:51:09 GMT

←Older revision		Revision as of 15:51, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of a alanine91 mutant of WCI==		==Crystal structure of a alanine91 mutant of WCI==
	<StructureSection load='4tlp' size='340' side='right' caption='[[4tlp]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4tlp' size='340' side='right' caption='[[4tlp]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4tlp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TLP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tlp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TLP FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tlp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tlp RCSB], [http://www.ebi.ac.uk/pdbsum/4tlp PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tlp OCA], [http://pdbe.org/4tlp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tlp RCSB], [http://www.ebi.ac.uk/pdbsum/4tlp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tlp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4tlp" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Chymotrypsin Inhibitor\|Chymotrypsin Inhibitor]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:56, 24 December 2014

OCA — Wed, 24 Dec 2014 10:56:55 GMT

←Older revision		Revision as of 10:56, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4tlp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TLP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tlp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TLP FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tlp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tlp RCSB], [http://www.ebi.ac.uk/pdbsum/4tlp PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tlp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tlp RCSB], [http://www.ebi.ac.uk/pdbsum/4tlp PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ICW3_PSOTE ICW3_PSOTE]] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	beta-trefoil fold, consisting of a six stranded beta-barrel capped at one end by a lid comprising of another six beta-strands, is one of the most important folds among proteins. Important classes of proteins like Interleukins (ILs), Fibroblast Growth Factors (FGFs), Kunitz (STI) family of inhibitors etc. belong to this fold. Their core is packed by hydrophobic residues contributed by the 6 stranded beta-barrel and three beta-hairpins that make essential contacts with each other and keep the protein in 'topologically minimal frustrated state'. A complete database analysis of the core residues of the beta-trefoil fold proteins presented here identified a conserved tryptophan (W91) residue in the Kunitz (STI) family of inhibitors that projects from the lid and interacts with the bottom layer residues of the barrel. This kind of interactions is unique in Kunitz (STI) family because no other families of beta-trefoil fold have such a shear sized residue at the barrel lid junction; suggesting its possible importance in packing and stability. We took WCI as a representative of this family and prepared four cavity creating mutants W91F-WCI, W91M-WCI, W91I-WCI & W91A-WCI. CD experiments show that the secondary structure of the mutants remains indistinguishable with the wild type. Crystal structures of the mutants W91F-WCI, W91M-WCI & W91A-WCI also show the same feature. However, slight readjustments of the side chains around the site of mutation have been observed so as to minimize the cavity created due to mutation. Comparative stability of these mutants, estimated using heat denaturation CD spectroscopy, indicates that stability of the mutants inversely correlates with the size of the cavity inside the core. Interestingly, although we mutated at the core, mutants show varying susceptibility against tryptic digestion that grossly follow their instability determined by CD. Our findings suggest that the W91 residue plays an important role in determining the stability and packing of the core of WCI.
		+
		+	A conserved tryptophan (W91) at the barrel-lid junction modulates the packing and stability of Kunitz (STI) family of inhibitors.,Majumder S, Khamrui S, Banerjee R, Bhowmik P, Sen U Biochim Biophys Acta. 2015 Jan;1854(1):55-64. doi: 10.1016/j.bbapap.2014.10.021. , Epub 2014 Oct 31. PMID:25448016<ref>PMID:25448016</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Majumder, S.]]	+	[[Category: Majumder, S]]
-	[[Category: Sen, U.]]	+	[[Category: Sen, U]]
	[[Category: Chymotrypsin inhibitor]]		[[Category: Chymotrypsin inhibitor]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Inhibitor]]		[[Category: Inhibitor]]
	[[Category: Tryptophan]]		[[Category: Tryptophan]]

OCA at 03:01, 7 August 2014

OCA — Thu, 07 Aug 2014 03:01:41 GMT

←Older revision		Revision as of 03:01, 7 August 2014
Line 7:		Line 7:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Majumder,S]]	+	[[Category: Majumder, S.]]
-	[[Category: Sen,U.]]	+	[[Category: Sen, U.]]
	[[Category: Chymotrypsin inhibitor]]		[[Category: Chymotrypsin inhibitor]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Inhibitor]]		[[Category: Inhibitor]]
	[[Category: Tryptophan]]		[[Category: Tryptophan]]

OCA at 08:56, 2 July 2014

OCA — Wed, 02 Jul 2014 08:56:01 GMT

←Older revision		Revision as of 08:56, 2 July 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of a alanine91 mutant of WCI==
-		+	<StructureSection load='4tlp' size='340' side='right' caption='[[4tlp]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~The entry~~ 4tlp is ~~ON HOLD~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4tlp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TLP FirstGlance]. <br>
-	~~Authors~~: ~~Sen~~,U., Majumder,S	+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tlp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tlp RCSB], [http://www.ebi.ac.uk/pdbsum/4tlp PDBsum]</span></td></tr>
-		+	<table>
-	~~Description~~: ~~Crystal structure of a alanine91 mutant of WCI~~	+	__TOC__
		+	</StructureSection>
		+	[[Category: Majumder,S]]
		+	[[Category: Sen,U.]]
		+	[[Category: Chymotrypsin inhibitor]]
		+	[[Category: Hydrolase]]
		+	[[Category: Inhibitor]]
		+	[[Category: Tryptophan]]

OCA: Protected "4tlp" [edit=sysop:move=sysop]

OCA — Wed, 04 Jun 2014 05:00:20 GMT

Protected "4tlp" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:00, 4 June 2014

OCA: New page: '''Unreleased structure''' The entry 4tlp is ON HOLD Authors: Sen,U., Majumder,S Description: Crystal structure of a alanine91 mutant of WCI

OCA — Wed, 04 Jun 2014 05:00:19 GMT

New page: '''Unreleased structure''' The entry 4tlp is ON HOLD Authors: Sen,U., Majumder,S Description: Crystal structure of a alanine91 mutant of WCI

New page

'''Unreleased structure'''

The entry 4tlp is ON HOLD

Authors: Sen,U., Majumder,S

Description: Crystal structure of a alanine91 mutant of WCI