4tnq - Revision history

OCA at 07:20, 27 September 2023

OCA — Wed, 27 Sep 2023 07:20:26 GMT

←Older revision		Revision as of 07:20, 27 September 2023
Line 3:		Line 3:
	<StructureSection load='4tnq' size='340' side='right'caption='[[4tnq]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='4tnq' size='340' side='right'caption='[[4tnq]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4tnq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TNQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4tnq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TNQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GTP:GUANOSINE~~-~~5-TRIPHOSPHATE'>GTP</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.55Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[4tnp\|4tnp]], [[4tnr\|4tnr]], [[4tnx\|4tnx]], [[4tny\|4tny]], [[4tnz\|4tnz]], [[4to0\|4to0]], [[4to1\|4to1]], [[4to2\|4to2]], [[4to3\|4to3]], [[4to4\|4to4]], [[4to5\|4to5]], [[4to6\|4to6]]~~</~~div~~><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">SAMHD1, MOP5 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnq OCA], [https://pdbe.org/4tnq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tnq RCSB], [https://www.ebi.ac.uk/pdbsum/4tnq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnq OCA], [https://pdbe.org/4tnq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tnq RCSB], [https://www.ebi.ac.uk/pdbsum/4tnq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnq ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[https://www.uniprot.org/uniprot/SAMH1_HUMAN SAMH1_HUMAN]] Defects in SAMHD1 are the cause of Aicardi-Goutieres syndrome type 5 (AGS5) [MIM:[https://omim.org/entry/612952 612952]]. A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood.<ref>PMID:19525956</ref> <ref>PMID:20842748</ref> Defects in SAMHD1 are the cause of chilblain lupus type 2 (CHBL2) [MIM:[https://omim.org/entry/614415 614415]]. A rare cutaneous form of lupus erythematosus. Affected individuals present with painful bluish-red papular or nodular lesions of the skin in acral locations precipitated by cold and wet exposure at temperatures less than 10 degrees centigrade.<ref>PMID:21204240</ref>	+	[https://www.uniprot.org/uniprot/SAMH1_HUMAN SAMH1_HUMAN] Defects in SAMHD1 are the cause of Aicardi-Goutieres syndrome type 5 (AGS5) [MIM:[https://omim.org/entry/612952 612952]. A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood.<ref>PMID:19525956</ref> <ref>PMID:20842748</ref> Defects in SAMHD1 are the cause of chilblain lupus type 2 (CHBL2) [MIM:[https://omim.org/entry/614415 614415]. A rare cutaneous form of lupus erythematosus. Affected individuals present with painful bluish-red papular or nodular lesions of the skin in acral locations precipitated by cold and wet exposure at temperatures less than 10 degrees centigrade.<ref>PMID:21204240</ref>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SAMH1_HUMAN SAMH1_HUMAN]] Putative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling.<ref>PMID:18546154</ref> <ref>PMID:19525956</ref>	+	[https://www.uniprot.org/uniprot/SAMH1_HUMAN SAMH1_HUMAN] Putative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling.<ref>PMID:18546154</ref> <ref>PMID:19525956</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ji, X]]	+	[[Category: Ji X]]
-	[[Category: Tang, C]]	+	[[Category: Tang C]]
-	[[Category: Wang, W]]	+	[[Category: Wang W]]
-	[[Category: Xiong, Y]]	+	[[Category: Xiong Y]]
-	[[Category: Zhao, Q]]	+	[[Category: Zhao Q]]
-	~~[[Category: Dntp regulation]]~~	+
-	~~[[Category: Dntpase]]~~	+
-	~~[[Category: Hiv]]~~	+
-	~~[[Category: Host pathogen interaction]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Restriction factor]]~~	+
-	~~[[Category: Samhd1~~]]	+

OCA at 10:51, 30 March 2022

OCA — Wed, 30 Mar 2022 10:51:04 GMT

OCA at 14:49, 10 May 2019

OCA — Fri, 10 May 2019 14:49:30 GMT

←Older revision		Revision as of 14:49, 10 May 2019
Line 1:		Line 1:

	==Structural basis of cellular dNTP regulation, SAMHD1-GTP-dTTP-dTTP complex==		==Structural basis of cellular dNTP regulation, SAMHD1-GTP-dTTP-dTTP complex==
-	<StructureSection load='4tnq' size='340' side='right' caption='[[4tnq]], [[Resolution\|resolution]] 2.55Å' scene=''>	+	<StructureSection load='4tnq' size='340' side='right'caption='[[4tnq]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4tnq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TNQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4tnq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TNQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tnp\|4tnp]], [[4tnr\|4tnr]], [[4tnx\|4tnx]], [[4tny\|4tny]], [[4tnz\|4tnz]], [[4to0\|4to0]], [[4to1\|4to1]], [[4to2\|4to2]], [[4to3\|4to3]], [[4to4\|4to4]], [[4to5\|4to5]], [[4to6\|4to6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tnp\|4tnp]], [[4tnr\|4tnr]], [[4tnx\|4tnx]], [[4tny\|4tny]], [[4tnz\|4tnz]], [[4to0\|4to0]], [[4to1\|4to1]], [[4to2\|4to2]], [[4to3\|4to3]], [[4to4\|4to4]], [[4to5\|4to5]], [[4to6\|4to6]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAMHD1, MOP5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnq OCA], [http://pdbe.org/4tnq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tnq RCSB], [http://www.ebi.ac.uk/pdbsum/4tnq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnq OCA], [http://pdbe.org/4tnq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tnq RCSB], [http://www.ebi.ac.uk/pdbsum/4tnq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnq ProSAT]</span></td></tr>
	</table>		</table>
Line 25:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Ji, X]]		[[Category: Ji, X]]
	[[Category: Tang, C]]		[[Category: Tang, C]]
Line 34:		Line 37:
	[[Category: Hiv]]		[[Category: Hiv]]
	[[Category: Host pathogen interaction]]		[[Category: Host pathogen interaction]]
		+	[[Category: Hydrolase]]
	[[Category: Restriction factor]]		[[Category: Restriction factor]]
	[[Category: Samhd1]]		[[Category: Samhd1]]

OCA at 14:43, 11 August 2016

OCA — Thu, 11 Aug 2016 14:43:07 GMT

←Older revision		Revision as of 14:43, 11 August 2016
Line 1:		Line 1:
		+
	==Structural basis of cellular dNTP regulation, SAMHD1-GTP-dTTP-dTTP complex==		==Structural basis of cellular dNTP regulation, SAMHD1-GTP-dTTP-dTTP complex==
	<StructureSection load='4tnq' size='340' side='right' caption='[[4tnq]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='4tnq' size='340' side='right' caption='[[4tnq]], [[Resolution\|resolution]] 2.55Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tnp\|4tnp]], [[4tnr\|4tnr]], [[4tnx\|4tnx]], [[4tny\|4tny]], [[4tnz\|4tnz]], [[4to0\|4to0]], [[4to1\|4to1]], [[4to2\|4to2]], [[4to3\|4to3]], [[4to4\|4to4]], [[4to5\|4to5]], [[4to6\|4to6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tnp\|4tnp]], [[4tnr\|4tnr]], [[4tnx\|4tnx]], [[4tny\|4tny]], [[4tnz\|4tnz]], [[4to0\|4to0]], [[4to1\|4to1]], [[4to2\|4to2]], [[4to3\|4to3]], [[4to4\|4to4]], [[4to5\|4to5]], [[4to6\|4to6]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tnq RCSB], [http://www.ebi.ac.uk/pdbsum/4tnq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnq OCA], [http://pdbe.org/4tnq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tnq RCSB], [http://www.ebi.ac.uk/pdbsum/4tnq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnq ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4tnq" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:17, 25 January 2015

OCA — Sun, 25 Jan 2015 09:17:15 GMT

←Older revision		Revision as of 09:17, 25 January 2015
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Ji, X.]]	+	[[Category: Ji, X]]
-	[[Category: Tang, C.]]	+	[[Category: Tang, C]]
-	[[Category: Wang, W.]]	+	[[Category: Wang, W]]
-	[[Category: Xiong, Y.]]	+	[[Category: Xiong, Y]]
-	[[Category: Zhao, Q.]]	+	[[Category: Zhao, Q]]
	[[Category: Dntp regulation]]		[[Category: Dntp regulation]]
	[[Category: Dntpase]]		[[Category: Dntpase]]

OCA at 08:48, 20 October 2014

OCA — Mon, 20 Oct 2014 08:48:30 GMT

←Older revision		Revision as of 08:48, 20 October 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4tnq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TNQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tnq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TNQ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tnp\|4tnp]], [[4tnr\|4tnr]], [[4tnx\|4tnx]], [[4tny\|4tny]], [[4tnz\|4tnz]], [[4to0\|4to0]], [[4to1\|4to1]], [[4to2\|4to2]], [[4to3\|4to3]], [[4to4\|4to4]], [[4to5\|4to5]], [[4to6\|4to6]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tnp\|4tnp]], [[4tnr\|4tnr]], [[4tnx\|4tnx]], [[4tny\|4tny]], [[4tnz\|4tnz]], [[4to0\|4to0]], [[4to1\|4to1]], [[4to2\|4to2]], [[4to3\|4to3]], [[4to4\|4to4]], [[4to5\|4to5]], [[4to6\|4to6]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tnq RCSB], [http://www.ebi.ac.uk/pdbsum/4tnq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tnq RCSB], [http://www.ebi.ac.uk/pdbsum/4tnq PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Disease ==		== Disease ==
	[[http://www.uniprot.org/uniprot/SAMH1_HUMAN SAMH1_HUMAN]] Defects in SAMHD1 are the cause of Aicardi-Goutieres syndrome type 5 (AGS5) [MIM:[http://omim.org/entry/612952 612952]]. A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood.<ref>PMID:19525956</ref> <ref>PMID:20842748</ref> Defects in SAMHD1 are the cause of chilblain lupus type 2 (CHBL2) [MIM:[http://omim.org/entry/614415 614415]]. A rare cutaneous form of lupus erythematosus. Affected individuals present with painful bluish-red papular or nodular lesions of the skin in acral locations precipitated by cold and wet exposure at temperatures less than 10 degrees centigrade.<ref>PMID:21204240</ref>		[[http://www.uniprot.org/uniprot/SAMH1_HUMAN SAMH1_HUMAN]] Defects in SAMHD1 are the cause of Aicardi-Goutieres syndrome type 5 (AGS5) [MIM:[http://omim.org/entry/612952 612952]]. A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood.<ref>PMID:19525956</ref> <ref>PMID:20842748</ref> Defects in SAMHD1 are the cause of chilblain lupus type 2 (CHBL2) [MIM:[http://omim.org/entry/614415 614415]]. A rare cutaneous form of lupus erythematosus. Affected individuals present with painful bluish-red papular or nodular lesions of the skin in acral locations precipitated by cold and wet exposure at temperatures less than 10 degrees centigrade.<ref>PMID:21204240</ref>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/SAMH1_HUMAN SAMH1_HUMAN]] Putative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling.<ref>PMID:18546154</ref> <ref>PMID:19525956</ref>		[[http://www.uniprot.org/uniprot/SAMH1_HUMAN SAMH1_HUMAN]] Putative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling.<ref>PMID:18546154</ref> <ref>PMID:19525956</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The sterile alpha motif and HD domain-containing protein 1 (SAMHD1), a dNTPase, prevents the infection of nondividing cells by retroviruses, including HIV, by depleting the cellular dNTP pool available for viral reverse transcription. SAMHD1 is a major regulator of cellular dNTP levels in mammalian cells. Mutations in SAMHD1 are associated with chronic lymphocytic leukemia (CLL) and the autoimmune condition Aicardi Goutieres syndrome (AGS). The dNTPase activity of SAMHD1 can be regulated by dGTP, with which SAMHD1 assembles into catalytically active tetramers. Here we present extensive biochemical and structural data that reveal an exquisite activation mechanism of SAMHD1 via combined action of both GTP and dNTPs. We obtained 26 crystal structures of SAMHD1 in complex with different combinations of GTP and dNTP mixtures, which depict the full spectrum of GTP/dNTP binding at the eight allosteric and four catalytic sites of the SAMHD1 tetramer. Our data demonstrate how SAMHD1 is activated by binding of GTP or dGTP at allosteric site 1 and a dNTP of any type at allosteric site 2. Our enzymatic assays further reveal a robust regulatory mechanism of SAMHD1 activity, which bares resemblance to that of the ribonuclease reductase responsible for cellular dNTP production. These results establish a complete framework for a mechanistic understanding of the important functions of SAMHD1 in the regulation of cellular dNTP levels, as well as in HIV restriction and the pathogenesis of CLL and AGS.
		+
		+	Structural basis of cellular dNTP regulation by SAMHD1.,Ji X, Tang C, Zhao Q, Wang W, Xiong Y Proc Natl Acad Sci U S A. 2014 Oct 14;111(41):E4305-14. doi:, 10.1073/pnas.1412289111. Epub 2014 Sep 29. PMID:25267621<ref>PMID:25267621</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 00:28, 2 October 2014

OCA — Thu, 02 Oct 2014 00:28:25 GMT

←Older revision		Revision as of 00:28, 2 October 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Structural basis of cellular dNTP regulation, SAMHD1-GTP-dTTP-dTTP complex==
-		+	<StructureSection load='4tnq' size='340' side='right' caption='[[4tnq]], [[Resolution\|resolution]] 2.55Å' scene=''>
-	~~The entry~~ 4tnq is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4tnq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TNQ FirstGlance]. <br>
-	~~Authors~~: Ji, X., ~~Tang~~, C., ~~Zhao~~, Q., Wang, W., Xiong, Y.	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene><br>
-		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tnp\|4tnp]], [[4tnr\|4tnr]], [[4tnx\|4tnx]], [[4tny\|4tny]], [[4tnz\|4tnz]], [[4to0\|4to0]], [[4to1\|4to1]], [[4to2\|4to2]], [[4to3\|4to3]], [[4to4\|4to4]], [[4to5\|4to5]], [[4to6\|4to6]]</td></tr>
-	~~Description~~: ~~Structural basis of cellular dNTP~~ regulation~~, SAMHD1-GTP-dTTP-dTTP complex~~	+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tnq RCSB], [http://www.ebi.ac.uk/pdbsum/4tnq PDBsum]</span></td></tr>
		+	<table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/SAMH1_HUMAN SAMH1_HUMAN]] Defects in SAMHD1 are the cause of Aicardi-Goutieres syndrome type 5 (AGS5) [MIM:[http://omim.org/entry/612952 612952]]. A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood.<ref>PMID:19525956</ref> <ref>PMID:20842748</ref> Defects in SAMHD1 are the cause of chilblain lupus type 2 (CHBL2) [MIM:[http://omim.org/entry/614415 614415]]. A rare cutaneous form of lupus erythematosus. Affected individuals present with painful bluish-red papular or nodular lesions of the skin in acral locations precipitated by cold and wet exposure at temperatures less than 10 degrees centigrade.<ref>PMID:21204240</ref>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SAMH1_HUMAN SAMH1_HUMAN]] Putative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling.<ref>PMID:18546154</ref> <ref>PMID:19525956</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ji, X.]]
		+	[[Category: Tang, C.]]
		+	[[Category: Wang, W.]]
		+	[[Category: Xiong, Y.]]
		+	[[Category: Zhao, Q.]]
		+	[[Category: Dntp regulation]]
		+	[[Category: Dntpase]]
		+	[[Category: Hiv]]
		+	[[Category: Host pathogen interaction]]
		+	[[Category: Restriction factor]]
		+	[[Category: Samhd1]]

OCA at 06:00, 25 June 2014

OCA — Wed, 25 Jun 2014 06:00:53 GMT

←Older revision		Revision as of 06:00, 25 June 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4tnq is ON HOLD	+	The entry 4tnq is ON HOLD until Paper Publication

	Authors: Ji, X., Tang, C., Zhao, Q., Wang, W., Xiong, Y.		Authors: Ji, X., Tang, C., Zhao, Q., Wang, W., Xiong, Y.

	Description: Structural basis of cellular dNTP regulation, SAMHD1-GTP-dTTP-dTTP complex		Description: Structural basis of cellular dNTP regulation, SAMHD1-GTP-dTTP-dTTP complex

OCA at 06:43, 18 June 2014

OCA — Wed, 18 Jun 2014 06:43:56 GMT

←Older revision		Revision as of 06:43, 18 June 2014
Line 5:		Line 5:
	Authors: Ji, X., Tang, C., Zhao, Q., Wang, W., Xiong, Y.		Authors: Ji, X., Tang, C., Zhao, Q., Wang, W., Xiong, Y.

-	Description: Structural basis of cellular dNTP regulation, SAMHD1-GTP-dTTP complex	+	Description: Structural basis of cellular dNTP regulation, SAMHD1-GTP-dTTP-dTTP complex

OCA: Protected "4tnq" [edit=sysop:move=sysop]

OCA — Wed, 11 Jun 2014 08:15:09 GMT

Protected "4tnq" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:15, 11 June 2014

←Older revision		Revision as of 10:51, 30 March 2022
Line 3:		Line 3:
	<StructureSection load='4tnq' size='340' side='right'caption='[[4tnq]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='4tnq' size='340' side='right'caption='[[4tnq]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4tnq]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4TNQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4tnq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TNQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tnp\|4tnp]], [[4tnr\|4tnr]], [[4tnx\|4tnx]], [[4tny\|4tny]], [[4tnz\|4tnz]], [[4to0\|4to0]], [[4to1\|4to1]], [[4to2\|4to2]], [[4to3\|4to3]], [[4to4\|4to4]], [[4to5\|4to5]], [[4to6\|4to6]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4tnp\|4tnp]], [[4tnr\|4tnr]], [[4tnx\|4tnx]], [[4tny\|4tny]], [[4tnz\|4tnz]], [[4to0\|4to0]], [[4to1\|4to1]], [[4to2\|4to2]], [[4to3\|4to3]], [[4to4\|4to4]], [[4to5\|4to5]], [[4to6\|4to6]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAMHD1, MOP5 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAMHD1, MOP5 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4tnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnq OCA], [~~http~~://pdbe.org/4tnq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4tnq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4tnq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnq OCA], [https://pdbe.org/4tnq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tnq RCSB], [https://www.ebi.ac.uk/pdbsum/4tnq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnq ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[~~http~~://www.uniprot.org/uniprot/SAMH1_HUMAN SAMH1_HUMAN]] Defects in SAMHD1 are the cause of Aicardi-Goutieres syndrome type 5 (AGS5) [MIM:[~~http~~://omim.org/entry/612952 612952]]. A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood.<ref>PMID:19525956</ref> <ref>PMID:20842748</ref> Defects in SAMHD1 are the cause of chilblain lupus type 2 (CHBL2) [MIM:[~~http~~://omim.org/entry/614415 614415]]. A rare cutaneous form of lupus erythematosus. Affected individuals present with painful bluish-red papular or nodular lesions of the skin in acral locations precipitated by cold and wet exposure at temperatures less than 10 degrees centigrade.<ref>PMID:21204240</ref>	+	[[https://www.uniprot.org/uniprot/SAMH1_HUMAN SAMH1_HUMAN]] Defects in SAMHD1 are the cause of Aicardi-Goutieres syndrome type 5 (AGS5) [MIM:[https://omim.org/entry/612952 612952]]. A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood.<ref>PMID:19525956</ref> <ref>PMID:20842748</ref> Defects in SAMHD1 are the cause of chilblain lupus type 2 (CHBL2) [MIM:[https://omim.org/entry/614415 614415]]. A rare cutaneous form of lupus erythematosus. Affected individuals present with painful bluish-red papular or nodular lesions of the skin in acral locations precipitated by cold and wet exposure at temperatures less than 10 degrees centigrade.<ref>PMID:21204240</ref>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SAMH1_HUMAN SAMH1_HUMAN]] Putative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling.<ref>PMID:18546154</ref> <ref>PMID:19525956</ref>	+	[[https://www.uniprot.org/uniprot/SAMH1_HUMAN SAMH1_HUMAN]] Putative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling.<ref>PMID:18546154</ref> <ref>PMID:19525956</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==