4to9 - Revision history

OCA at 07:21, 27 September 2023

OCA — Wed, 27 Sep 2023 07:21:43 GMT

←Older revision		Revision as of 07:21, 27 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4to9]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TO9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TO9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4to9]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TO9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TO9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4to9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4to9 OCA], [https://pdbe.org/4to9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4to9 RCSB], [https://www.ebi.ac.uk/pdbsum/4to9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4to9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4to9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4to9 OCA], [https://pdbe.org/4to9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4to9 RCSB], [https://www.ebi.ac.uk/pdbsum/4to9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4to9 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:47, 15 March 2023

OCA — Wed, 15 Mar 2023 10:47:44 GMT

←Older revision		Revision as of 10:47, 15 March 2023
Line 3:		Line 3:
	<StructureSection load='4to9' size='340' side='right'caption='[[4to9]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4to9' size='340' side='right'caption='[[4to9]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4to9]] is a 24 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TO9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4TO9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4to9]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TO9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TO9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4toa\|4toa]], [[4tob\|4tob]], [[4toc\|4toc]], [[4tod\|4tod]], [[4toe\|4toe]], [[4tof\|4tof]], [[4tog\|4tog]], [[4toh\|4toh]], [[3is7\|3is7]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4to9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4to9 OCA], [https://pdbe.org/4to9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4to9 RCSB], [https://www.ebi.ac.uk/pdbsum/4to9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4to9 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bfrB, PA3531 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4to9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4to9 OCA], [~~http~~://pdbe.org/4to9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4to9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4to9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4to9 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/Q9HY79_PSEAE Q9HY79_PSEAE]] Iron-storage protein (By similarity). Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex (By similarity).[PIRNR:PIRNR002560]	+	[https://www.uniprot.org/uniprot/Q9HY79_PSEAE Q9HY79_PSEAE] Iron-storage protein (By similarity). Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex (By similarity).[PIRNR:PIRNR002560]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Ferroxidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pseae~~]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: Battaile~~, K P~~]]	+	[[Category: Battaile KP]]
-	[[Category: Eshelman, K]]	+	[[Category: Eshelman K]]
-	[[Category: Kumar, R]]	+	[[Category: Kumar R]]
-	[[Category: Lovell, S]]	+	[[Category: Lovell S]]
-	[[Category: Rivera, M]]	+	[[Category: Rivera M]]
-	[[Category: Yao, H]]	+	[[Category: Yao H]]
-	~~[[Category: Electron transport]]~~	+
-	~~[[Category: Iron binding]]~~	+
-	~~[[Category: Iron mobilization]]~~	+
-	~~[[Category: Iron storage]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 07:17, 27 November 2019

OCA — Wed, 27 Nov 2019 07:17:13 GMT

←Older revision		Revision as of 07:17, 27 November 2019
Line 1:		Line 1:

	==2.0A resolution structure of BfrB (N148L) from Pseudomonas aeruginosa==		==2.0A resolution structure of BfrB (N148L) from Pseudomonas aeruginosa==
-	<StructureSection load='4to9' size='340' side='right' caption='[[4to9]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4to9' size='340' side='right'caption='[[4to9]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4to9]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TO9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TO9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4to9]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TO9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TO9 FirstGlance]. <br>
Line 21:		Line 21:
	</div>		</div>
	<div class="pdbe-citations 4to9" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4to9" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ferritin 3D structures\|Ferritin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Ferroxidase]]		[[Category: Ferroxidase]]
		+	[[Category: Large Structures]]
	[[Category: Pseae]]		[[Category: Pseae]]
	[[Category: Battaile, K P]]		[[Category: Battaile, K P]]

OCA at 14:56, 15 November 2017

OCA — Wed, 15 Nov 2017 14:56:24 GMT

←Older revision		Revision as of 14:56, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='4to9' size='340' side='right' caption='[[4to9]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4to9' size='340' side='right' caption='[[4to9]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4to9]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TO9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TO9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4to9]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TO9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TO9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4toa\|4toa]], [[4tob\|4tob]], [[4toc\|4toc]], [[4tod\|4tod]], [[4toe\|4toe]], [[4tof\|4tof]], [[4tog\|4tog]], [[4toh\|4toh]], [[3is7\|3is7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4toa\|4toa]], [[4tob\|4tob]], [[4toc\|4toc]], [[4tod\|4tod]], [[4toe\|4toe]], [[4tof\|4tof]], [[4tog\|4tog]], [[4toh\|4toh]], [[3is7\|3is7]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bfrB, PA3531 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4to9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4to9 OCA], [http://pdbe.org/4to9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4to9 RCSB], [http://www.ebi.ac.uk/pdbsum/4to9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4to9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4to9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4to9 OCA], [http://pdbe.org/4to9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4to9 RCSB], [http://www.ebi.ac.uk/pdbsum/4to9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4to9 ProSAT]</span></td></tr>
Line 25:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Ferroxidase]]		[[Category: Ferroxidase]]
		+	[[Category: Pseae]]
	[[Category: Battaile, K P]]		[[Category: Battaile, K P]]
	[[Category: Eshelman, K]]		[[Category: Eshelman, K]]

OCA at 01:14, 19 January 2017

OCA — Thu, 19 Jan 2017 01:14:32 GMT

←Older revision		Revision as of 01:14, 19 January 2017
Line 1:		Line 1:
		+
	==2.0A resolution structure of BfrB (N148L) from Pseudomonas aeruginosa==		==2.0A resolution structure of BfrB (N148L) from Pseudomonas aeruginosa==
	<StructureSection load='4to9' size='340' side='right' caption='[[4to9]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4to9' size='340' side='right' caption='[[4to9]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4toa\|4toa]], [[4tob\|4tob]], [[4toc\|4toc]], [[4tod\|4tod]], [[4toe\|4toe]], [[4tof\|4tof]], [[4tog\|4tog]], [[4toh\|4toh]], [[3is7\|3is7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4toa\|4toa]], [[4tob\|4tob]], [[4toc\|4toc]], [[4tod\|4tod]], [[4toe\|4toe]], [[4tof\|4tof]], [[4tog\|4tog]], [[4toh\|4toh]], [[3is7\|3is7]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4to9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4to9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4to9 RCSB], [http://www.ebi.ac.uk/pdbsum/4to9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4to9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4to9 OCA], [http://pdbe.org/4to9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4to9 RCSB], [http://www.ebi.ac.uk/pdbsum/4to9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4to9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4to9" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:53, 12 February 2015

OCA — Thu, 12 Feb 2015 11:53:37 GMT

←Older revision		Revision as of 11:53, 12 February 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==2.0A resolution structure of BfrB (N148L) from Pseudomonas aeruginosa==
		+	<StructureSection load='4to9' size='340' side='right' caption='[[4to9]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4to9]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TO9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TO9 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4toa\|4toa]], [[4tob\|4tob]], [[4toc\|4toc]], [[4tod\|4tod]], [[4toe\|4toe]], [[4tof\|4tof]], [[4tog\|4tog]], [[4toh\|4toh]], [[3is7\|3is7]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4to9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4to9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4to9 RCSB], [http://www.ebi.ac.uk/pdbsum/4to9 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q9HY79_PSEAE Q9HY79_PSEAE]] Iron-storage protein (By similarity). Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex (By similarity).[PIRNR:PIRNR002560]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	X-ray crystallography, molecular dynamics (MD) simulations and biochemistry were utilized to investigate the effect of introducing hydrophobic interactions in the 4-fold (N148L and Q151L) and B-pores (D34F) of Pseudomonas aeruginosa bacterioferritin B (BfrB) on BfrB function. The structures show only local structural perturbations and confirm the anticipated hydrophobic interactions. Surprisingly, structures obtained after soaking crystals in Fe2+-containing crystallization solution revealed that although iron loads into the ferroxidase centers of the mutants, the side chains of ferroxidase ligands E51 and H130 do not reorganize to bind the iron ions, as is seen in the wt BfrB structures. Similar experiments with a double mutant (C89S/K96C) prepared to introduce changes outside the pores show competent ferroxidase centers that function akin to those in wt BfrB. MD simulations comparing wt BfrB with the D34F and N148L mutants show that the mutants exhibit significantly reduced flexibility, and reveal a network of concerted motions linking ferroxidase centers and 4-fold and B-pores, which are important for imparting ferroxidase centers in BfrB with the required flexibility to function efficiently. In agreement, the efficiency of Fe2+ oxidation and uptake of the 4-fold and B-pore mutants in solution is significantly compromised relative to wt or C89S/K96C BfrB. Finally, our structures show a large number of previously unknown iron binding sites in the interior cavity and B-pores of BfrB, which reveal in unprecedented detail conduits followed by iron and phosphate ions across the BfrB shell, as well as paths in the interior cavity that may facilitate nucleation of the iron phosphate mineral.

-	~~The entry 4to9 is ON HOLD until Paper Publication~~	+	Concerted Motions Networking Pores and Distant Ferroxidase Centers Enable Bacterioferritin Function and Iron Traffic.,Yao H, Rui H, Kumar R, Eshelman K, Lovell S, Battaile KP, Im W, Rivera M Biochemistry. 2015 Jan 31. PMID:25640193<ref>PMID:25640193</ref>

-	~~Authors: Lovell~~, S.~~, Battaile, K.P., Yao, H., Kumar, R., Eshelman, K., Rivera, M~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~2.0A resolution structure of Apo BfrB (N148L) from Pseudomonas aeruginosa~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: ~~Rivera~~, M]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Ferroxidase]]
		+	[[Category: Battaile, K P]]
		+	[[Category: Eshelman, K]]
	[[Category: Kumar, R]]		[[Category: Kumar, R]]
-	[[Category: Battaile, K.P]]
	[[Category: Lovell, S]]		[[Category: Lovell, S]]
-	[[Category: ~~Eshelman~~, K]]	+	[[Category: Rivera, M]]
	[[Category: Yao, H]]		[[Category: Yao, H]]
		+	[[Category: Electron transport]]
		+	[[Category: Iron binding]]
		+	[[Category: Iron mobilization]]
		+	[[Category: Iron storage]]
		+	[[Category: Oxidoreductase]]

OCA at 17:44, 24 December 2014

OCA — Wed, 24 Dec 2014 17:44:34 GMT

←Older revision		Revision as of 17:44, 24 December 2014
Line 6:		Line 6:

	Description: 2.0A resolution structure of Apo BfrB (N148L) from Pseudomonas aeruginosa		Description: 2.0A resolution structure of Apo BfrB (N148L) from Pseudomonas aeruginosa
		+	[[Category: Unreleased Structures]]
		+	[[Category: Rivera, M]]
		+	[[Category: Kumar, R]]
		+	[[Category: Battaile, K.P]]
		+	[[Category: Lovell, S]]
		+	[[Category: Eshelman, K]]
		+	[[Category: Yao, H]]

OCA at 06:01, 25 June 2014

OCA — Wed, 25 Jun 2014 06:01:22 GMT

←Older revision		Revision as of 06:01, 25 June 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4to9 is ON HOLD	+	The entry 4to9 is ON HOLD until Paper Publication

	Authors: Lovell, S., Battaile, K.P., Yao, H., Kumar, R., Eshelman, K., Rivera, M.		Authors: Lovell, S., Battaile, K.P., Yao, H., Kumar, R., Eshelman, K., Rivera, M.

	Description: 2.0A resolution structure of Apo BfrB (N148L) from Pseudomonas aeruginosa		Description: 2.0A resolution structure of Apo BfrB (N148L) from Pseudomonas aeruginosa

OCA at 06:44, 18 June 2014

OCA — Wed, 18 Jun 2014 06:44:19 GMT

←Older revision		Revision as of 06:44, 18 June 2014
Line 3:		Line 3:
	The entry 4to9 is ON HOLD		The entry 4to9 is ON HOLD

-	Authors: Lovell, ~~Scott~~, Battaile, ~~Kevin~~ P., Yao, ~~Huili~~, Kumar, ~~Ritesh~~, Eshelman, ~~Kate~~, Rivera, ~~Mario~~	+	Authors: Lovell, S., Battaile, K.P., Yao, H., Kumar, R., Eshelman, K., Rivera, M.

	Description: 2.0A resolution structure of Apo BfrB (N148L) from Pseudomonas aeruginosa		Description: 2.0A resolution structure of Apo BfrB (N148L) from Pseudomonas aeruginosa

OCA: Protected "4to9" [edit=sysop:move=sysop]

OCA — Wed, 11 Jun 2014 08:16:02 GMT

Protected "4to9" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:16, 11 June 2014