4tpa - Revision history

OCA: Redirecting to 4u26

OCA — Wed, 10 Dec 2014 15:52:46 GMT

Redirecting to 4u26

←Older revision		Revision as of 15:52, 10 December 2014
Line 1:		Line 1:
-	~~==Crystal structure of the E. coli ribosome bound to dalfopristin and quinupristin. This file contains the 30S subunit of the second 70S ribosome.==~~	+	#REDIRECT [[4u26]] This PDB entry is obsolete and replaced by 4u26
-	~~<StructureSection load='4tpa' size='340' side='right' caption='~~[[~~4tpa]], [[Resolution\|resolution]] 2.80Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[4tpa~~]] is ~~a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]~~ and [http://en.wikipedia.org/wiki/Escherichia_coli_str._k-12_substr._mds42 Escherichia coli str. k-12 substr. mds42]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TPA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TPA FirstGlance]. <br>	+
-	~~</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>~~	+
-	~~<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tp8\|4tp8]], [[4tp9\|4tp9]], [[4tpb\|4tpb]]</td></tr>~~	+
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tpa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tpa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tpa RCSB], [http://www.ebi.ac.uk/pdbsum/4tpa PDBsum]</span></td></tr>	+
-	~~<table>~~	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Streptogramin antibiotics are divided into type A and B streptogramins, which in combination can act synergistically. We compared the molecular interactions of the streptogramin combinations Synercid (type A: dalfopristin, type B: quinupristin) and NXL 103 (type A: flopristin, type B: linopristin) with the Escherichia coli 70S ribosome by x-ray crystallography. We further analyzed the activity of the streptogramin components individually and in combination. Streptogramin A and B components in Synercid and NXL 103 exhibit synergistic antimicrobial activity against certain pathogenic bacteria. However, in transcription-coupled translation assays, only combinations that include dalfopristin, the streptogramin A component of Synercid, show synergy. Notably, the diethylaminoethylsulfonyl group in dalfopristin reduces its activity, but is the basis for synergy in transcription-coupled translation assays before its rapid hydrolysis from the depsipeptide core. Replacement of the diethylaminoethylsulfonyl group in dalfopristin by a non-hydrolyzable group may therefore be beneficial for synergy. The absence of general streptogramin synergy in transcription-coupled translation assays suggests that synergistic antimicrobial activity of streptogramins can occur independently of streptogramin effects on translation.	+
-		+
-	Synergy of streptogramin antibiotics occurs independently of their effects on translation.,Noeske J, Huang J, Olivier NB, Giacobbe RA, Zambrowski M, Cate JH Antimicrob Agents Chemother. 2014 Jun 23. pii: AAC.03389-14. PMID:24957822<ref>PMID:24957822</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Escherichia coli]]~~	+
-	~~[[Category: Escherichia coli str. k-12 substr. mds42]]~~	+
-	~~[[Category: Cate, J H.D.]]~~	+
-	~~[[Category: Giacobbe, R A.]]~~	+
-	~~[[Category: Huang, J.]]~~	+
-	~~[[Category: Noeske, J.]]~~	+
-	~~[[Category: Olivier, N B.]]~~	+
-	~~[[Category: Zambrowski, M.]]~~	+
-	~~[[Category: 16]]~~	+
-	~~[[Category: 30]]~~	+
-	~~[[Category: 70]]~~	+
-	~~[[Category: Antibiotic]]~~	+
-	~~[[Category: Exit]]~~	+
-	~~[[Category: Peptidyl]]~~	+
-	~~[[Category: Protein biosynthesis]]~~	+
-	~~[[Category: Ribosomal subunit]]~~	+
-	~~[[Category: Ribosome]]~~	+
-	~~[[Category: Rna]]~~	+
-	~~[[Category: Streptogramin]]~~	+
-	~~[[Category: Transfer]]~~	+

OCA at 07:39, 30 July 2014

OCA — Wed, 30 Jul 2014 07:39:48 GMT

←Older revision		Revision as of 07:39, 30 July 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of the E. coli ribosome bound to dalfopristin and quinupristin. This file contains the 30S subunit of the second 70S ribosome.==
		+	<StructureSection load='4tpa' size='340' side='right' caption='[[4tpa]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4tpa]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Escherichia_coli_str._k-12_substr._mds42 Escherichia coli str. k-12 substr. mds42]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TPA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TPA FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tp8\|4tp8]], [[4tp9\|4tp9]], [[4tpb\|4tpb]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tpa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tpa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tpa RCSB], [http://www.ebi.ac.uk/pdbsum/4tpa PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Streptogramin antibiotics are divided into type A and B streptogramins, which in combination can act synergistically. We compared the molecular interactions of the streptogramin combinations Synercid (type A: dalfopristin, type B: quinupristin) and NXL 103 (type A: flopristin, type B: linopristin) with the Escherichia coli 70S ribosome by x-ray crystallography. We further analyzed the activity of the streptogramin components individually and in combination. Streptogramin A and B components in Synercid and NXL 103 exhibit synergistic antimicrobial activity against certain pathogenic bacteria. However, in transcription-coupled translation assays, only combinations that include dalfopristin, the streptogramin A component of Synercid, show synergy. Notably, the diethylaminoethylsulfonyl group in dalfopristin reduces its activity, but is the basis for synergy in transcription-coupled translation assays before its rapid hydrolysis from the depsipeptide core. Replacement of the diethylaminoethylsulfonyl group in dalfopristin by a non-hydrolyzable group may therefore be beneficial for synergy. The absence of general streptogramin synergy in transcription-coupled translation assays suggests that synergistic antimicrobial activity of streptogramins can occur independently of streptogramin effects on translation.

-	~~The entry 4tpa is ON HOLD~~	+	Synergy of streptogramin antibiotics occurs independently of their effects on translation.,Noeske J, Huang J, Olivier NB, Giacobbe RA, Zambrowski M, Cate JH Antimicrob Agents Chemother. 2014 Jun 23. pii: AAC.03389-14. PMID:24957822<ref>PMID:24957822</ref>

-	~~Authors~~: ~~Noeske~~, J., Huang, J., Olivier, N.B.~~, Giacobbe, R.A.,~~ Zambrowski, M.~~, Cate, J.H.D.~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of the E. coli ribosome bound to dalfopristin and quinupristin. This file contains the 30S~~ subunit ~~of the second 70S ribosome.~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Escherichia coli str. k-12 substr. mds42]]
		+	[[Category: Cate, J H.D.]]
		+	[[Category: Giacobbe, R A.]]
		+	[[Category: Huang, J.]]
		+	[[Category: Noeske, J.]]
		+	[[Category: Olivier, N B.]]
		+	[[Category: Zambrowski, M.]]
		+	[[Category: 16]]
		+	[[Category: 30]]
		+	[[Category: 70]]
		+	[[Category: Antibiotic]]
		+	[[Category: Exit]]
		+	[[Category: Peptidyl]]
		+	[[Category: Protein biosynthesis]]
		+	[[Category: Ribosomal subunit]]
		+	[[Category: Ribosome]]
		+	[[Category: Rna]]
		+	[[Category: Streptogramin]]
		+	[[Category: Transfer]]

OCA: Protected "4tpa" [edit=sysop:move=sysop]

OCA — Wed, 18 Jun 2014 06:45:47 GMT

Protected "4tpa" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:45, 18 June 2014

OCA: New page: '''Unreleased structure''' The entry 4tpa is ON HOLD Authors: Noeske, J., Huang, J., Olivier, N.B., Giacobbe, R.A., Zambrowski, M., Cate, J.H.D. Description: Crystal structure of the E...

OCA — Wed, 18 Jun 2014 06:45:46 GMT

New page: '''Unreleased structure''' The entry 4tpa is ON HOLD Authors: Noeske, J., Huang, J., Olivier, N.B., Giacobbe, R.A., Zambrowski, M., Cate, J.H.D. Description: Crystal structure of the E...

New page

'''Unreleased structure'''

The entry 4tpa is ON HOLD

Authors: Noeske, J., Huang, J., Olivier, N.B., Giacobbe, R.A., Zambrowski, M., Cate, J.H.D.

Description: Crystal structure of the E. coli ribosome bound to dalfopristin and quinupristin. This file contains the 30S subunit of the second 70S ribosome.