4tsk - Revision history

OCA at 07:22, 27 September 2023

OCA — Wed, 27 Sep 2023 07:22:53 GMT

←Older revision		Revision as of 07:22, 27 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4tsk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Alicyclobacillus_acidocaldarius_subsp._acidocaldarius_DSM_446 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TSK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TSK FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tsk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Alicyclobacillus_acidocaldarius_subsp._acidocaldarius_DSM_446 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TSK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TSK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tsk OCA], [https://pdbe.org/4tsk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tsk RCSB], [https://www.ebi.ac.uk/pdbsum/4tsk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tsk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tsk OCA], [https://pdbe.org/4tsk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tsk RCSB], [https://www.ebi.ac.uk/pdbsum/4tsk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tsk ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:53, 15 March 2023

OCA — Wed, 15 Mar 2023 10:53:39 GMT

←Older revision		Revision as of 10:53, 15 March 2023
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	==Ketol-acid reductoisomerase from Alicyclobacillus acidocaldarius==		==Ketol-acid reductoisomerase from Alicyclobacillus acidocaldarius==
-	<StructureSection load='4tsk' size='340' side='right' caption='[[4tsk]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='4tsk' size='340' side='right'caption='[[4tsk]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4tsk]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aliad Aliad~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TSK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4TSK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4tsk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Alicyclobacillus_acidocaldarius_subsp._acidocaldarius_DSM_446 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TSK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TSK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ilvC, Aaci_2227 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=521098 ALIAD])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tsk OCA], [https://pdbe.org/4tsk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tsk RCSB], [https://www.ebi.ac.uk/pdbsum/4tsk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tsk ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ketol-acid_reductoisomerase Ketol-acid reductoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.86 1.1.1.86] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4tsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tsk OCA], [~~http~~://pdbe.org/4tsk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4tsk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4tsk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4tsk ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ILVC_ALIAD ILVC_ALIAD] Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.[HAMAP-Rule:MF_00435]<ref>PMID:25849365</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4tsk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4tsk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ketol-acid reductoisomerase 3D structures\|Ketol-acid reductoisomerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aliad~~]]	+	[[Category: Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446]]
-	[[Category: ~~Ketol-acid reductoisomerase~~]]	+	[[Category: Large Structures]]
-	[[Category: Arnold~~, F H~~]]	+	[[Category: Arnold FH]]
-	[[Category: Brinkmann-Chen, S]]	+	[[Category: Brinkmann-Chen S]]
-	[[Category: Cahn~~, J K.B]]~~	+	[[Category: Cahn JKB]]
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Nadph-binding]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Rossmann fold~~]]	+

OCA at 09:17, 22 November 2017

OCA — Wed, 22 Nov 2017 09:17:17 GMT

←Older revision		Revision as of 09:17, 22 November 2017
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	<StructureSection load='4tsk' size='340' side='right' caption='[[4tsk]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4tsk' size='340' side='right' caption='[[4tsk]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4tsk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TSK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TSK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4tsk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aliad Aliad]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TSK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TSK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ilvC, Aaci_2227 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=521098 ALIAD])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ketol-acid_reductoisomerase Ketol-acid reductoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.86 1.1.1.86] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ketol-acid_reductoisomerase Ketol-acid reductoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.86 1.1.1.86] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tsk OCA], [http://pdbe.org/4tsk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tsk RCSB], [http://www.ebi.ac.uk/pdbsum/4tsk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tsk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tsk OCA], [http://pdbe.org/4tsk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tsk RCSB], [http://www.ebi.ac.uk/pdbsum/4tsk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tsk ProSAT]</span></td></tr>
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	</div>		</div>
	<div class="pdbe-citations 4tsk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4tsk" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Ketol-acid reductoisomerase\|Ketol-acid reductoisomerase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aliad]]
	[[Category: Ketol-acid reductoisomerase]]		[[Category: Ketol-acid reductoisomerase]]
	[[Category: Arnold, F H]]		[[Category: Arnold, F H]]

OCA at 19:59, 11 August 2016

OCA — Thu, 11 Aug 2016 19:59:07 GMT

←Older revision		Revision as of 19:59, 11 August 2016
Line 1:		Line 1:
		+
	==Ketol-acid reductoisomerase from Alicyclobacillus acidocaldarius==		==Ketol-acid reductoisomerase from Alicyclobacillus acidocaldarius==
	<StructureSection load='4tsk' size='340' side='right' caption='[[4tsk]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4tsk' size='340' side='right' caption='[[4tsk]], [[Resolution\|resolution]] 2.50Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ketol-acid_reductoisomerase Ketol-acid reductoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.86 1.1.1.86] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ketol-acid_reductoisomerase Ketol-acid reductoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.86 1.1.1.86] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tsk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tsk RCSB], [http://www.ebi.ac.uk/pdbsum/4tsk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tsk OCA], [http://pdbe.org/4tsk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tsk RCSB], [http://www.ebi.ac.uk/pdbsum/4tsk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tsk ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4tsk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ketol-acid reductoisomerase\|Ketol-acid reductoisomerase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:53, 19 November 2014

OCA — Wed, 19 Nov 2014 10:53:12 GMT

←Older revision		Revision as of 10:53, 19 November 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4tsk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TSK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TSK FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tsk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TSK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TSK FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ketol-acid_reductoisomerase Ketol-acid reductoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.86 1.1.1.86] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ketol-acid_reductoisomerase Ketol-acid reductoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.86 1.1.1.86] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tsk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tsk RCSB], [http://www.ebi.ac.uk/pdbsum/4tsk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tsk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tsk RCSB], [http://www.ebi.ac.uk/pdbsum/4tsk PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	All members of the ketol-acid reductoisomerase (KARI) enzyme family characterized to date have been shown to prefer the nicotinamide adenine dinucleotide phosphate hydride (NADPH) cofactor to nicotinamide adenine dinucleotide hydride (NADH). However, KARIs with the reversed cofactor preference are desirable for industrial applications, including anaerobic fermentation to produce branched-chain amino acids. By applying insights gained from structural and engineering studies of this enzyme family to a comprehensive multiple sequence alignment of KARIs, we identified putative NADH-utilizing KARIs and characterized eight whose catalytic efficiencies using NADH were equal to or greater than NADPH. These are the first naturally NADH-preferring KARIs reported and demonstrate that this property has evolved independently multiple times, using strategies unlike those used previously in the laboratory to engineer a KARI cofactor switch.
		+
		+	Uncovering rare NADH-preferring ketol-acid reductoisomerases.,Brinkmann-Chen S, Cahn JK, Arnold FH Metab Eng. 2014 Aug 27;26C:17-22. doi: 10.1016/j.ymben.2014.08.003. PMID:25172159<ref>PMID:25172159</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Ketol-acid reductoisomerase]]		[[Category: Ketol-acid reductoisomerase]]
-	[[Category: Arnold, F H.]]	+	[[Category: Arnold, F H]]
-	[[Category: Brinkmann-Chen, S.]]	+	[[Category: Brinkmann-Chen, S]]
-	[[Category: Cahn, J K.B.]]	+	[[Category: Cahn, J K.B]]
	[[Category: Isomerase]]		[[Category: Isomerase]]
-	[[Category: Ketol-acid reductoisomerase]]
	[[Category: Nadph-binding]]		[[Category: Nadph-binding]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]

OCA at 07:36, 9 July 2014

OCA — Wed, 09 Jul 2014 07:36:42 GMT

←Older revision		Revision as of 07:36, 9 July 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Ketol-acid reductoisomerase from Alicyclobacillus acidocaldarius==
-		+	<StructureSection load='4tsk' size='340' side='right' caption='[[4tsk]], [[Resolution\|resolution]] 2.50Å' scene=''>
-	~~The entry~~ 4tsk is ~~ON HOLD~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4tsk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TSK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TSK FirstGlance]. <br>
-	~~Authors~~: ~~Cahn~~, J.K.B., ~~Brinkmann~~-~~Chen~~, S., Arnold, F.H.	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene><br>
-		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ketol-acid_reductoisomerase Ketol-acid reductoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.86 1.1.1.86] </span></td></tr>
-	~~Description~~: Ketol-acid reductoisomerase ~~from Alicyclobacillus acidocaldarius~~	+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tsk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tsk RCSB], [http://www.ebi.ac.uk/pdbsum/4tsk PDBsum]</span></td></tr>
		+	<table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ketol-acid reductoisomerase]]
		+	[[Category: Arnold, F H.]]
		+	[[Category: Brinkmann-Chen, S.]]
		+	[[Category: Cahn, J K.B.]]
		+	[[Category: Isomerase]]
		+	[[Category: Ketol-acid reductoisomerase]]
		+	[[Category: Nadph-binding]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Rossmann fold]]

OCA: Protected "4tsk" [edit=sysop:move=sysop]

OCA — Wed, 25 Jun 2014 06:05:00 GMT

Protected "4tsk" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:05, 25 June 2014

OCA: New page: '''Unreleased structure''' The entry 4tsk is ON HOLD Authors: Cahn, J.K.B., Brinkmann-Chen, S., Arnold, F.H. Description: Ketol-acid reductoisomerase from Alicyclobacillus acidocaldari...

OCA — Wed, 25 Jun 2014 06:04:59 GMT

New page: '''Unreleased structure''' The entry 4tsk is ON HOLD Authors: Cahn, J.K.B., Brinkmann-Chen, S., Arnold, F.H. Description: Ketol-acid reductoisomerase from Alicyclobacillus acidocaldari...

New page

'''Unreleased structure'''

The entry 4tsk is ON HOLD

Authors: Cahn, J.K.B., Brinkmann-Chen, S., Arnold, F.H.

Description: Ketol-acid reductoisomerase from Alicyclobacillus acidocaldarius