4txz - Revision history

OCA at 00:48, 28 December 2023

OCA — Thu, 28 Dec 2023 00:48:36 GMT

←Older revision		Revision as of 00:48, 28 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4txz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TXZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TXZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4txz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TXZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TXZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4txz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4txz OCA], [https://pdbe.org/4txz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4txz RCSB], [https://www.ebi.ac.uk/pdbsum/4txz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4txz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4txz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4txz OCA], [https://pdbe.org/4txz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4txz RCSB], [https://www.ebi.ac.uk/pdbsum/4txz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4txz ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:51, 22 March 2023

OCA — Wed, 22 Mar 2023 07:51:32 GMT

←Older revision		Revision as of 07:51, 22 March 2023
Line 3:		Line 3:
	<StructureSection load='4txz' size='340' side='right'caption='[[4txz]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='4txz' size='340' side='right'caption='[[4txz]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4txz]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Vibch Vibch~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TXZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4TXZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4txz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TXZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TXZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dncV, VC_0179 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243277 VIBCH])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4txz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4txz OCA], [https://pdbe.org/4txz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4txz RCSB], [https://www.ebi.ac.uk/pdbsum/4txz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4txz ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclic_GMP-AMP_synthase Cyclic GMP-AMP synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.86 2.7.7.86] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4txz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4txz OCA], [~~http~~://pdbe.org/4txz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4txz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4txz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4txz ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/DNCV_VIBCH DNCV_VIBCH]] Catalyzes the synthesis of cyclic AMP-GMP from ATP and GTP. Is also able to produce c-di-AMP and c-di-GMP from ATP and GTP, respectively; however, c-AMP-GMP is the dominant molecule produced by DncV in vivo. Is required for efficient V.cholerae intestinal colonization, and down-regulates the colonization-influencing process of chemotaxis. Is not active with dATP, TTP, UTP, and CTP.	+	[https://www.uniprot.org/uniprot/DNCV_VIBCH DNCV_VIBCH] Catalyzes the synthesis of cyclic AMP-GMP from ATP and GTP. Is also able to produce c-di-AMP and c-di-GMP from ATP and GTP, respectively; however, c-AMP-GMP is the dominant molecule produced by DncV in vivo. Is required for efficient V.cholerae intestinal colonization, and down-regulates the colonization-influencing process of chemotaxis. Is not active with dATP, TTP, UTP, and CTP.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cyclic GMP-AMP synthase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Vibch~~]]	+	[[Category: Vibrio cholerae O1 biovar El Tor str. N16961]]
-	[[Category: Berger~~, J M~~]]	+	[[Category: Berger JM]]
-	[[Category: Doudna~~, J A~~]]	+	[[Category: Doudna JA]]
-	[[Category: Kranzusch~~, P J~~]]	+	[[Category: Kranzusch PJ]]
-	[[Category: Lee~~, A S.Y~~]]	+	[[Category: Lee ASY]]
-	[[Category: Solovykh~~, M S~~]]	+	[[Category: Solovykh MS]]
-	[[Category: Vance~~, R E~~]]	+	[[Category: Vance RE]]
-	[[Category: Wilson~~, S C]]~~	+	[[Category: Wilson SC]]
-	~~[[Category: Cga]]~~	+
-	~~[[Category: Cyclic nucleotide synthase]]~~	+
-	~~[[Category: Nucleotidyl transferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 16:00, 20 November 2019

OCA — Wed, 20 Nov 2019 16:00:58 GMT

←Older revision		Revision as of 16:00, 20 November 2019
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Cyclic GMP-AMP synthase\|Cyclic GMP-AMP synthase]]	+	*[[Cyclic GMP-AMP synthase 3D synthase\|Cyclic GMP-AMP synthase 3D synthase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:44, 10 May 2019

OCA — Fri, 10 May 2019 14:44:35 GMT

←Older revision		Revision as of 14:44, 10 May 2019
Line 1:		Line 1:

	==Crystal structure of Vibrio cholerae DncV cyclic AMP-GMP synthase in complex with nonhydrolyzable GTP==		==Crystal structure of Vibrio cholerae DncV cyclic AMP-GMP synthase in complex with nonhydrolyzable GTP==
-	<StructureSection load='4txz' size='340' side='right' caption='[[4txz]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='4txz' size='340' side='right'caption='[[4txz]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4txz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TXZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TXZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4txz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibch Vibch]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TXZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TXZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dncV, VC_0179 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243277 VIBCH])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclic_GMP-AMP_synthase Cyclic GMP-AMP synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.86 2.7.7.86] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclic_GMP-AMP_synthase Cyclic GMP-AMP synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.86 2.7.7.86] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4txz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4txz OCA], [http://pdbe.org/4txz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4txz RCSB], [http://www.ebi.ac.uk/pdbsum/4txz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4txz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4txz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4txz OCA], [http://pdbe.org/4txz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4txz RCSB], [http://www.ebi.ac.uk/pdbsum/4txz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4txz ProSAT]</span></td></tr>
Line 27:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Cyclic GMP-AMP synthase]]		[[Category: Cyclic GMP-AMP synthase]]
		+	[[Category: Large Structures]]
		+	[[Category: Vibch]]
	[[Category: Berger, J M]]		[[Category: Berger, J M]]
	[[Category: Doudna, J A]]		[[Category: Doudna, J A]]
Line 37:		Line 40:
	[[Category: Cyclic nucleotide synthase]]		[[Category: Cyclic nucleotide synthase]]
	[[Category: Nucleotidyl transferase]]		[[Category: Nucleotidyl transferase]]
		+	[[Category: Transferase]]

OCA at 19:11, 11 August 2016

OCA — Thu, 11 Aug 2016 19:11:27 GMT

←Older revision		Revision as of 19:11, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Vibrio cholerae DncV cyclic AMP-GMP synthase in complex with nonhydrolyzable GTP==		==Crystal structure of Vibrio cholerae DncV cyclic AMP-GMP synthase in complex with nonhydrolyzable GTP==
	<StructureSection load='4txz' size='340' side='right' caption='[[4txz]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='4txz' size='340' side='right' caption='[[4txz]], [[Resolution\|resolution]] 2.80Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclic_GMP-AMP_synthase Cyclic GMP-AMP synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.86 2.7.7.86] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclic_GMP-AMP_synthase Cyclic GMP-AMP synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.86 2.7.7.86] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4txz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4txz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4txz RCSB], [http://www.ebi.ac.uk/pdbsum/4txz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4txz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4txz OCA], [http://pdbe.org/4txz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4txz RCSB], [http://www.ebi.ac.uk/pdbsum/4txz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4txz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4txz" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cyclic GMP-AMP synthase\|Cyclic GMP-AMP synthase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:58, 25 December 2014

OCA — Thu, 25 Dec 2014 13:58:09 GMT

←Older revision		Revision as of 13:58, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4txz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TXZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TXZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4txz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TXZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TXZ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclic_GMP-AMP_synthase Cyclic GMP-AMP synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.86 2.7.7.86] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclic_GMP-AMP_synthase Cyclic GMP-AMP synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.86 2.7.7.86] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4txz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4txz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4txz RCSB], [http://www.ebi.ac.uk/pdbsum/4txz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4txz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4txz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4txz RCSB], [http://www.ebi.ac.uk/pdbsum/4txz PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DNCV_VIBCH DNCV_VIBCH]] Catalyzes the synthesis of cyclic AMP-GMP from ATP and GTP. Is also able to produce c-di-AMP and c-di-GMP from ATP and GTP, respectively; however, c-AMP-GMP is the dominant molecule produced by DncV in vivo. Is required for efficient V.cholerae intestinal colonization, and down-regulates the colonization-influencing process of chemotaxis. Is not active with dATP, TTP, UTP, and CTP.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Cyclic GMP-AMP synthase]]		[[Category: Cyclic GMP-AMP synthase]]
-	[[Category: Berger, J M.]]	+	[[Category: Berger, J M]]
-	[[Category: Doudna, J A.]]	+	[[Category: Doudna, J A]]
-	[[Category: Kranzusch, P J.]]	+	[[Category: Kranzusch, P J]]
-	[[Category: Lee, A S.Y.]]	+	[[Category: Lee, A S.Y]]
-	[[Category: Solovykh, M S.]]	+	[[Category: Solovykh, M S]]
-	[[Category: Vance, R E.]]	+	[[Category: Vance, R E]]
-	[[Category: Wilson, S C.]]	+	[[Category: Wilson, S C]]
	[[Category: Cga]]		[[Category: Cga]]
	[[Category: Cyclic nucleotide synthase]]		[[Category: Cyclic nucleotide synthase]]
	[[Category: Nucleotidyl transferase]]		[[Category: Nucleotidyl transferase]]

OCA at 03:10, 4 September 2014

OCA — Thu, 04 Sep 2014 03:10:07 GMT

←Older revision		Revision as of 03:10, 4 September 2014
Line 7:		Line 7:
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4txz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4txz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4txz RCSB], [http://www.ebi.ac.uk/pdbsum/4txz PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4txz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4txz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4txz RCSB], [http://www.ebi.ac.uk/pdbsum/4txz PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cyclic dinucleotides (CDNs) play central roles in bacterial pathogenesis and innate immunity. The mammalian enzyme cGAS synthesizes a unique cyclic dinucleotide (cGAMP) containing a 2'-5' phosphodiester linkage essential for optimal immune stimulation, but the molecular basis for linkage specificity is unknown. Here, we show that the Vibrio cholerae pathogenicity factor DncV is a prokaryotic cGAS-like enzyme whose activity provides a mechanistic rationale for the unique ability of cGAS to produce 2'-5' cGAMP. Three high-resolution crystal structures show that DncV and human cGAS generate CDNs in sequential reactions that proceed in opposing directions. We explain 2' and 3' linkage specificity and test this model by reprogramming the human cGAS active site to produce 3'-5' cGAMP, leading to selective stimulation of alternative STING adaptor alleles in cells. These results demonstrate mechanistic homology between bacterial signaling and mammalian innate immunity and explain how active site configuration controls linkage chemistry for pathway-specific signaling.
		+
		+	Structure-Guided Reprogramming of Human cGAS Dinucleotide Linkage Specificity.,Kranzusch PJ, Lee AS, Wilson SC, Solovykh MS, Vance RE, Berger JM, Doudna JA Cell. 2014 Aug 28;158(5):1011-21. doi: 10.1016/j.cell.2014.07.028. Epub 2014 Aug , 14. PMID:25131990<ref>PMID:25131990</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 09:19, 13 August 2014

OCA — Wed, 13 Aug 2014 09:19:18 GMT

←Older revision		Revision as of 09:19, 13 August 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of Vibrio cholerae DncV cyclic AMP-GMP synthase in complex with nonhydrolyzable GTP==
-		+	<StructureSection load='4txz' size='340' side='right' caption='[[4txz]], [[Resolution\|resolution]] 2.80Å' scene=''>
-	~~The entry~~ 4txz is ~~ON HOLD~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4txz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TXZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TXZ FirstGlance]. <br>
-	~~Authors~~: ~~Kranzusch~~, P.J., ~~Lee, A~~.S.Y.~~, Wilson, S~~.C., ~~Solovykh, M~~.S., ~~Vance, R~~.E., Berger, J.M., Doudna, J.A.	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
-		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclic_GMP-AMP_synthase Cyclic GMP-AMP synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.86 2.7.7.86] </span></td></tr>
-	~~Description~~:	+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4txz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4txz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4txz RCSB], [http://www.ebi.ac.uk/pdbsum/4txz PDBsum]</span></td></tr>
		+	<table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cyclic GMP-AMP synthase]]
		+	[[Category: Berger, J M.]]
		+	[[Category: Doudna, J A.]]
		+	[[Category: Kranzusch, P J.]]
		+	[[Category: Lee, A S.Y.]]
		+	[[Category: Solovykh, M S.]]
		+	[[Category: Vance, R E.]]
		+	[[Category: Wilson, S C.]]
		+	[[Category: Cga]]
		+	[[Category: Cyclic nucleotide synthase]]
		+	[[Category: Nucleotidyl transferase]]

OCA: Protected "4txz" [edit=sysop:move=sysop]

OCA — Wed, 16 Jul 2014 09:56:55 GMT

Protected "4txz" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:56, 16 July 2014

OCA: New page: '''Unreleased structure''' The entry 4txz is ON HOLD Authors: Kranzusch, P.J., Lee, A.S.Y., Wilson, S.C., Solovykh, M.S., Vance, R.E., Berger, J.M., Doudna, J.A. Description:

OCA — Wed, 16 Jul 2014 09:56:55 GMT

New page: '''Unreleased structure''' The entry 4txz is ON HOLD Authors: Kranzusch, P.J., Lee, A.S.Y., Wilson, S.C., Solovykh, M.S., Vance, R.E., Berger, J.M., Doudna, J.A. Description:

New page

'''Unreleased structure'''

The entry 4txz is ON HOLD

Authors: Kranzusch, P.J., Lee, A.S.Y., Wilson, S.C., Solovykh, M.S., Vance, R.E., Berger, J.M., Doudna, J.A.

Description: