4tz5 - Revision history

OCA at 07:24, 27 September 2023

OCA — Wed, 27 Sep 2023 07:24:19 GMT

←Older revision		Revision as of 07:24, 27 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4tz5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._SirexAA-E Streptomyces sp. SirexAA-E]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TZ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TZ5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tz5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._SirexAA-E Streptomyces sp. SirexAA-E]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TZ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TZ5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tz5 OCA], [https://pdbe.org/4tz5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tz5 RCSB], [https://www.ebi.ac.uk/pdbsum/4tz5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tz5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tz5 OCA], [https://pdbe.org/4tz5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tz5 RCSB], [https://www.ebi.ac.uk/pdbsum/4tz5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tz5 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:52, 22 March 2023

OCA — Wed, 22 Mar 2023 07:52:50 GMT

←Older revision		Revision as of 07:52, 22 March 2023
Line 3:		Line 3:
	<StructureSection load='4tz5' size='340' side='right'caption='[[4tz5]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='4tz5' size='340' side='right'caption='[[4tz5]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4tz5]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Strek Strek~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TZ5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4TZ5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4tz5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._SirexAA-E Streptomyces sp. SirexAA-E]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TZ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TZ5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tyv\|4tyv]], [[4tz1\|4tz1]], [[4tz3\|4tz3]], [[4pf0\|4pf0]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tz5 OCA], [https://pdbe.org/4tz5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tz5 RCSB], [https://www.ebi.ac.uk/pdbsum/4tz5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tz5 ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SACTE_4363 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=862751 STREK])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4tz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tz5 OCA], [~~http~~://pdbe.org/4tz5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4tz5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4tz5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4tz5 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LAM55_STREK LAM55_STREK] Exo-beta-1,3-glucanase that specifically hydrolyzes laminarin and laminarioligosaccharides, producing glucose and laminaribiose as end products.<ref>PMID:25752603</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Strek~~]]	+	[[Category: Streptomyces sp. SirexAA-E]]
-	[[Category: Bergeman~~, L F~~]]	+	[[Category: Bergeman LF]]
-	[[Category: Bianchetti~~, C M~~]]	+	[[Category: Bianchetti CM]]
-	[[Category: Fox~~, B G~~]]	+	[[Category: Fox BG]]
-	[[Category: Takasuka~~, T E~~]]	+	[[Category: Takasuka TE]]
-	[[Category: Yik~~, E J]]~~	+	[[Category: Yik EJ]]
-	~~[[Category: 3-glucanase]]~~	+
-	~~[[Category: Beta-1]]~~	+
-	~~[[Category: Biomass degradation]]~~	+
-	~~[[Category: Exo-beta-1]]~~	+
-	~~[[Category: Gh55]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Laminaritetraose]]~~	+
-	~~[[Category: Secreted~~]]	+

OCA at 08:39, 4 December 2019

OCA — Wed, 04 Dec 2019 08:39:08 GMT

←Older revision		Revision as of 08:39, 4 December 2019
Line 1:		Line 1:
-	~~{{Large structure}}~~	+
	==Ensemble refinement of the E502A variant of sacteLam55A from Streptomyces sp. SirexAA-E in complex with laminarihexaose==		==Ensemble refinement of the E502A variant of sacteLam55A from Streptomyces sp. SirexAA-E in complex with laminarihexaose==
-	<StructureSection load='4tz5' size='340' side='right' caption='[[4tz5]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='4tz5' size='340' side='right'caption='[[4tz5]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4tz5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strek Strek]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TZ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TZ5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tz5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strek Strek]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TZ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TZ5 FirstGlance]. <br>
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tz5 OCA], [http://pdbe.org/4tz5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tz5 RCSB], [http://www.ebi.ac.uk/pdbsum/4tz5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tz5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tz5 OCA], [http://pdbe.org/4tz5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tz5 RCSB], [http://www.ebi.ac.uk/pdbsum/4tz5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tz5 ProSAT]</span></td></tr>
	</table>		</table>
-	{{Large structure}}
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Strek]]		[[Category: Strek]]
	[[Category: Bergeman, L F]]		[[Category: Bergeman, L F]]

OCA at 12:06, 6 November 2017

OCA — Mon, 06 Nov 2017 12:06:07 GMT

←Older revision		Revision as of 12:06, 6 November 2017
Line 3:		Line 3:
	<StructureSection load='4tz5' size='340' side='right' caption='[[4tz5]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='4tz5' size='340' side='right' caption='[[4tz5]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4tz5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TZ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TZ5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4tz5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strek Strek]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TZ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TZ5 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tyv\|4tyv]], [[4tz1\|4tz1]], [[4tz3\|4tz3]], [[4pf0\|4pf0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tyv\|4tyv]], [[4tz1\|4tz1]], [[4tz3\|4tz3]], [[4pf0\|4pf0]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SACTE_4363 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=862751 STREK])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tz5 OCA], [http://pdbe.org/4tz5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tz5 RCSB], [http://www.ebi.ac.uk/pdbsum/4tz5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tz5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tz5 OCA], [http://pdbe.org/4tz5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tz5 RCSB], [http://www.ebi.ac.uk/pdbsum/4tz5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tz5 ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Strek]]
	[[Category: Bergeman, L F]]		[[Category: Bergeman, L F]]
	[[Category: Bianchetti, C M]]		[[Category: Bianchetti, C M]]

OCA at 02:00, 10 March 2017

OCA — Fri, 10 Mar 2017 02:00:46 GMT

←Older revision		Revision as of 02:00, 10 March 2017
Line 1:		Line 1:
		+	{{Large structure}}
	==Ensemble refinement of the E502A variant of sacteLam55A from Streptomyces sp. SirexAA-E in complex with laminarihexaose==		==Ensemble refinement of the E502A variant of sacteLam55A from Streptomyces sp. SirexAA-E in complex with laminarihexaose==
	<StructureSection load='4tz5' size='340' side='right' caption='[[4tz5]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='4tz5' size='340' side='right' caption='[[4tz5]], [[Resolution\|resolution]] 1.75Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tyv\|4tyv]], [[4tz1\|4tz1]], [[4tz3\|4tz3]], [[4pf0\|4pf0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tyv\|4tyv]], [[4tz1\|4tz1]], [[4tz3\|4tz3]], [[4pf0\|4pf0]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tz5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tz5 RCSB], [http://www.ebi.ac.uk/pdbsum/4tz5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tz5 OCA], [http://pdbe.org/4tz5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tz5 RCSB], [http://www.ebi.ac.uk/pdbsum/4tz5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tz5 ProSAT]</span></td></tr>
	</table>		</table>
		+	{{Large structure}}
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4tz5" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:23, 26 March 2015

OCA — Thu, 26 Mar 2015 08:23:17 GMT

←Older revision		Revision as of 08:23, 26 March 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4tz5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TZ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TZ5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tz5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TZ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TZ5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~3B7~~:BETA-D-~~GLUCOPYRANOSYL-(1- 3)-BETA-D-GLUCOPYRANOSYL-(1- 3)-BETA-D-GLUCOPYRANOSYL-(1- 3)-BETA-D-GLUCOPYRANOSYL-(1- 3)-BETA-D-GLUCOPYRANOSYL-(1- 3)-BETA-D-GLUCOPYRANOSE~~'>~~3B7~~</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO~~</scene>, <scene name='pdbligand=LA5:BETA-D-GLUCOPYRANOSYL-(1- 3)-BETA-D-GLUCOPYRANOSYL-(1- 3)-BETA-D-GLUCOPYRANOSYL-(1- 3)-BETA-D-GLUCOPYRANOSYL-(1- 3)-BETA-D-GLUCOPYRANOSE'>LA5~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tyv\|4tyv]], [[4tz1\|4tz1]], [[4tz3\|4tz3]], [[4pf0\|4pf0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tyv\|4tyv]], [[4tz1\|4tz1]], [[4tz3\|4tz3]], [[4pf0\|4pf0]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tz5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tz5 RCSB], [http://www.ebi.ac.uk/pdbsum/4tz5 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tz5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tz5 RCSB], [http://www.ebi.ac.uk/pdbsum/4tz5 PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Carbohydrate Active Enzyme (CaZY) database indicates that glycoside hydrolase family 55 (GH55) contains both endo- and exo-beta -1,3-glucanases. The founding structure of the GH55 is PcLam55A from the white-rot fungus Phanaerochaete chrysosporium (Ishida, T., et al. (2009) J. Biol. Chem. 284, 10100-10109). Here, we present high resolution crystal structures of bacterial SacteLam55A from the highly cellulolytic Streptomyces sp. SirexAA-E with bound substrates and product. These structures, along with mutagenesis and kinetic studies implicate Glu502 as the catalytic acid (as proposed earlier for Glu663 in PcLam55A) and a proton relay network of four residues in activating water as the nucleophile. Further, a set of conserved aromatic residues that define the active site apparently enforce an exo-glucanase reactivity as demonstrated by exhaustive hydrolysis reactions with purified laminarioligosaccharides. Two additional aromatic residues that line the substrate-binding channel show substrate-dependent conformational flexibility that may promote processive reactivity of the bound oligosaccharide in the bacterial enzymes. Gene synthesis carried out on ~30% of the GH55 family gave 34 active enzymes (19% functional coverage of the non-redundant members of GH55). These active enzymes reacted with only laminarin from a panel of 10 different soluble and insoluble polysaccharides and displayed a broad range of specific activities, and optima for pH and temperature. Application of this experimental method provides a new, systematic way to annotate GH phylogenetic space for functional properties.
		+
		+	Active site and laminarin binding in glycoside hydrolase family 55.,Bianchetti CM, Takasuka TE, Deutsch S, Udell HS, Yik EJ, Bergeman LF, Fox BG J Biol Chem. 2015 Mar 9. pii: jbc.M114.623579. PMID:25752603<ref>PMID:25752603</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
Line 19:		Line 29:
	[[Category: Exo-beta-1]]		[[Category: Exo-beta-1]]
	[[Category: Gh55]]		[[Category: Gh55]]
		+	[[Category: Hydrolase]]
	[[Category: Laminaritetraose]]		[[Category: Laminaritetraose]]
	[[Category: Secreted]]		[[Category: Secreted]]

OCA at 12:15, 18 March 2015

OCA — Wed, 18 Mar 2015 12:15:03 GMT

←Older revision		Revision as of 12:15, 18 March 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Ensemble refinement of the E502A variant of sacteLam55A from Streptomyces sp. SirexAA-E in complex with laminarihexaose==
-		+	<StructureSection load='4tz5' size='340' side='right' caption='[[4tz5]], [[Resolution\|resolution]] 1.75Å' scene=''>
-	~~The entry~~ 4tz5 is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4tz5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TZ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TZ5 FirstGlance]. <br>
-	~~Authors~~: ~~Bianchetti~~, ~~C.M.~~, ~~Takasuka~~, T.E., ~~Yik, E~~.J., ~~Bergeman, L~~.F., ~~Fox, B~~.G.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3B7:BETA-D-GLUCOPYRANOSYL-(1- 3)-BETA-D-GLUCOPYRANOSYL-(1- 3)-BETA-D-GLUCOPYRANOSYL-(1- 3)-BETA-D-GLUCOPYRANOSYL-(1- 3)-BETA-D-GLUCOPYRANOSYL-(1- 3)-BETA-D-GLUCOPYRANOSE'>3B7</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=LA5:BETA-D-GLUCOPYRANOSYL-(1- 3)-BETA-D-GLUCOPYRANOSYL-(1- 3)-BETA-D-GLUCOPYRANOSYL-(1- 3)-BETA-D-GLUCOPYRANOSYL-(1- 3)-BETA-D-GLUCOPYRANOSE'>LA5</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tyv\|4tyv]], [[4tz1\|4tz1]], [[4tz3\|4tz3]], [[4pf0\|4pf0]]</td></tr>
-	~~Description: Ensemble refinement of the E502A variant of sacteLam55A from Streptomyces sp. SirexAA-E in complex with laminarihexaose~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tz5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tz5 RCSB], [http://www.ebi.ac.uk/pdbsum/4tz5 PDBsum]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: Bianchetti, C.M]]	+	__TOC__
-	[[Category: Yik, E.J]]	+	</StructureSection>
-	[[Category: ~~Bergeman, L.F~~]]	+	[[Category: Bergeman, L F]]
-	[[Category: ~~Fox, B.G~~]]	+	[[Category: Bianchetti, C M]]
-	[[Category: ~~Takasuka, T.E~~]]	+	[[Category: Fox, B G]]
		+	[[Category: Takasuka, T E]]
		+	[[Category: Yik, E J]]
		+	[[Category: 3-glucanase]]
		+	[[Category: Beta-1]]
		+	[[Category: Biomass degradation]]
		+	[[Category: Exo-beta-1]]
		+	[[Category: Gh55]]
		+	[[Category: Laminaritetraose]]
		+	[[Category: Secreted]]

OCA at 18:00, 24 December 2014

OCA — Wed, 24 Dec 2014 18:00:06 GMT

←Older revision		Revision as of 18:00, 24 December 2014
Line 6:		Line 6:

	Description: Ensemble refinement of the E502A variant of sacteLam55A from Streptomyces sp. SirexAA-E in complex with laminarihexaose		Description: Ensemble refinement of the E502A variant of sacteLam55A from Streptomyces sp. SirexAA-E in complex with laminarihexaose
		+	[[Category: Unreleased Structures]]
		+	[[Category: Bianchetti, C.M]]
		+	[[Category: Yik, E.J]]
		+	[[Category: Bergeman, L.F]]
		+	[[Category: Fox, B.G]]
		+	[[Category: Takasuka, T.E]]

OCA at 10:14, 29 October 2014

OCA — Wed, 29 Oct 2014 10:14:33 GMT

←Older revision		Revision as of 10:14, 29 October 2014
Line 5:		Line 5:
	Authors: Bianchetti, C.M., Takasuka, T.E., Yik, E.J., Bergeman, L.F., Fox, B.G.		Authors: Bianchetti, C.M., Takasuka, T.E., Yik, E.J., Bergeman, L.F., Fox, B.G.

-	Description: Ensemble refinement of the E502A ~~varient~~ of sacteLam55A from Streptomyces sp. SirexAA-E in complex with laminarihexaose	+	Description: Ensemble refinement of the E502A variant of sacteLam55A from Streptomyces sp. SirexAA-E in complex with laminarihexaose

OCA at 08:42, 24 September 2014

OCA — Wed, 24 Sep 2014 08:42:44 GMT

←Older revision		Revision as of 08:42, 24 September 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4tz5 is ON HOLD	+	The entry 4tz5 is ON HOLD until Paper Publication

	Authors: Bianchetti, C.M., Takasuka, T.E., Yik, E.J., Bergeman, L.F., Fox, B.G.		Authors: Bianchetti, C.M., Takasuka, T.E., Yik, E.J., Bergeman, L.F., Fox, B.G.

	Description: Ensemble refinement of the E502A varient of sacteLam55A from Streptomyces sp. SirexAA-E in complex with laminarihexaose		Description: Ensemble refinement of the E502A varient of sacteLam55A from Streptomyces sp. SirexAA-E in complex with laminarihexaose