4u2e - Revision history

OCA at 00:49, 28 December 2023

OCA — Thu, 28 Dec 2023 00:49:36 GMT

←Older revision		Revision as of 00:49, 28 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4u2e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_knackmussii Pseudomonas knackmussii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U2E FirstGlance]. <br>		<table><tr><td colspan='2'>[[4u2e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_knackmussii Pseudomonas knackmussii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U2E FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2e OCA], [https://pdbe.org/4u2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u2e RCSB], [https://www.ebi.ac.uk/pdbsum/4u2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u2e ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2e OCA], [https://pdbe.org/4u2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u2e RCSB], [https://www.ebi.ac.uk/pdbsum/4u2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u2e ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:58, 22 March 2023

OCA — Wed, 22 Mar 2023 07:58:27 GMT

←Older revision		Revision as of 07:58, 22 March 2023
Line 3:		Line 3:
	<StructureSection load='4u2e' size='340' side='right'caption='[[4u2e]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4u2e' size='340' side='right'caption='[[4u2e]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4u2e]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Dsm_6978 Dsm 6978~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2E OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4U2E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4u2e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_knackmussii Pseudomonas knackmussii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U2E FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4u2b\|4u2b]], [[4u2c\|4u2c]], [[4u2d\|4u2d]], [[4u2f\|4u2f]], [[4u2g\|4u2g]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2e OCA], [https://pdbe.org/4u2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u2e RCSB], [https://www.ebi.ac.uk/pdbsum/4u2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u2e ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">clcD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=65741 DSM 6978])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxymethylenebutenolidase Carboxymethylenebutenolidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.45 3.1.1.45] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4u2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2e OCA], [~~http~~://pdbe.org/4u2e PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4u2e RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4u2e PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4u2e ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~CLCD_PSESB CLCD_PSESB]~~] Ring cleavage of cyclic ester dienelactone to produce maleylacetate.	+	[https://www.uniprot.org/uniprot/CLCD_PSEKB CLCD_PSEKB] Ring cleavage of cyclic ester dienelactone to produce maleylacetate.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Carboxymethylenebutenolidase]]
-	[[Category: Dsm 6978]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Collyer, C A~~]]	+	[[Category: Pseudomonas knackmussii]]
-	[[Category: ~~Ollis, D L~~]]	+	[[Category: Collyer CA]]
-	[[Category: ~~Porter, J L~~]]	+	[[Category: Ollis DL]]
-	[[Category: ~~A/b hydrolase fold]]~~	+	[[Category: Porter JL]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 08:42, 1 January 2020

OCA — Wed, 01 Jan 2020 08:42:50 GMT

←Older revision		Revision as of 08:42, 1 January 2020
Line 1:		Line 1:

	==Crystal structure of dienelactone hydrolase S-3 variant (Q35H, F38L, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G, G211D and K234N) at 1.70 A resolution==		==Crystal structure of dienelactone hydrolase S-3 variant (Q35H, F38L, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G, G211D and K234N) at 1.70 A resolution==
-	<StructureSection load='4u2e' size='340' side='right' caption='[[4u2e]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='4u2e' size='340' side='right'caption='[[4u2e]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4u2e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_6978 Dsm 6978]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U2E FirstGlance]. <br>		<table><tr><td colspan='2'>[[4u2e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_6978 Dsm 6978]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U2E FirstGlance]. <br>
Line 27:		Line 27:
	[[Category: Carboxymethylenebutenolidase]]		[[Category: Carboxymethylenebutenolidase]]
	[[Category: Dsm 6978]]		[[Category: Dsm 6978]]
		+	[[Category: Large Structures]]
	[[Category: Collyer, C A]]		[[Category: Collyer, C A]]
	[[Category: Ollis, D L]]		[[Category: Ollis, D L]]

OCA at 09:19, 22 November 2017

OCA — Wed, 22 Nov 2017 09:19:49 GMT

←Older revision		Revision as of 09:19, 22 November 2017
Line 3:		Line 3:
	<StructureSection load='4u2e' size='340' side='right' caption='[[4u2e]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4u2e' size='340' side='right' caption='[[4u2e]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4u2e]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U2E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4u2e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_6978 Dsm 6978]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U2E FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4u2b\|4u2b]], [[4u2c\|4u2c]], [[4u2d\|4u2d]], [[4u2f\|4u2f]], [[4u2g\|4u2g]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4u2b\|4u2b]], [[4u2c\|4u2c]], [[4u2d\|4u2d]], [[4u2f\|4u2f]], [[4u2g\|4u2g]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">clcD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=65741 DSM 6978])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxymethylenebutenolidase Carboxymethylenebutenolidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.45 3.1.1.45] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxymethylenebutenolidase Carboxymethylenebutenolidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.45 3.1.1.45] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2e OCA], [http://pdbe.org/4u2e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4u2e RCSB], [http://www.ebi.ac.uk/pdbsum/4u2e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4u2e ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2e OCA], [http://pdbe.org/4u2e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4u2e RCSB], [http://www.ebi.ac.uk/pdbsum/4u2e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4u2e ProSAT]</span></td></tr>
Line 25:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Carboxymethylenebutenolidase]]		[[Category: Carboxymethylenebutenolidase]]
		+	[[Category: Dsm 6978]]
	[[Category: Collyer, C A]]		[[Category: Collyer, C A]]
	[[Category: Ollis, D L]]		[[Category: Ollis, D L]]

OCA at 01:14, 19 January 2017

OCA — Thu, 19 Jan 2017 01:14:42 GMT

←Older revision		Revision as of 01:14, 19 January 2017
Line 1:		Line 1:
		+
	==Crystal structure of dienelactone hydrolase S-3 variant (Q35H, F38L, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G, G211D and K234N) at 1.70 A resolution==		==Crystal structure of dienelactone hydrolase S-3 variant (Q35H, F38L, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G, G211D and K234N) at 1.70 A resolution==
	<StructureSection load='4u2e' size='340' side='right' caption='[[4u2e]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4u2e' size='340' side='right' caption='[[4u2e]], [[Resolution\|resolution]] 1.70Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4u2b\|4u2b]], [[4u2c\|4u2c]], [[4u2d\|4u2d]], [[4u2f\|4u2f]], [[4u2g\|4u2g]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4u2b\|4u2b]], [[4u2c\|4u2c]], [[4u2d\|4u2d]], [[4u2f\|4u2f]], [[4u2g\|4u2g]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxymethylenebutenolidase Carboxymethylenebutenolidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.45 3.1.1.45] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxymethylenebutenolidase Carboxymethylenebutenolidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.45 3.1.1.45] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u2e RCSB], [http://www.ebi.ac.uk/pdbsum/4u2e PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2e OCA], [http://pdbe.org/4u2e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4u2e RCSB], [http://www.ebi.ac.uk/pdbsum/4u2e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4u2e ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4u2e" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:14, 25 December 2014

OCA — Thu, 25 Dec 2014 10:14:17 GMT

←Older revision		Revision as of 10:14, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u2e RCSB], [http://www.ebi.ac.uk/pdbsum/4u2e PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u2e RCSB], [http://www.ebi.ac.uk/pdbsum/4u2e PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CLCD_PSESB CLCD_PSESB]] Ring cleavage of cyclic ester dienelactone to produce maleylacetate.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 16:07, 10 December 2014

OCA — Wed, 10 Dec 2014 16:07:35 GMT

←Older revision		Revision as of 16:07, 10 December 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of dienelactone hydrolase S-3 variant (Q35H, F38L, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G, G211D and K234N) at 1.70 A resolution==
		+	<StructureSection load='4u2e' size='340' side='right' caption='[[4u2e]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4u2e]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U2E FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4u2b\|4u2b]], [[4u2c\|4u2c]], [[4u2d\|4u2d]], [[4u2f\|4u2f]], [[4u2g\|4u2g]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxymethylenebutenolidase Carboxymethylenebutenolidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.45 3.1.1.45] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u2e RCSB], [http://www.ebi.ac.uk/pdbsum/4u2e PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The ease with which enzymes can be adapted from their native roles and engineered to function specifically for industrial or commercial applications is crucial to enabling enzyme technology to advance beyond its current state. Directed evolution is a powerful tool for engineering enzymes with improved physical and catalytic properties and can be used to evolve enzymes where lack of structural information may thwart the use of rational design. In this study, we take the versatile and diverse alpha/beta hydrolase fold framework, in the form of dienelactone hydrolase, and evolve it over three unique sequential evolutions with a total of 14 rounds of screening to generate a series of enzyme variants. The native enzyme has a low level of promiscuous activity toward p-nitrophenyl acetate but almost undetectable activity toward larger p-nitrophenyl esters. Using p-nitrophenyl acetate as an evolutionary intermediate, we have generated variants with altered specificity and catalytic activity up to 3 orders of magnitude higher than the native enzyme toward the larger nonphysiological p-nitrophenyl ester substrates. Several variants also possess increased stability resulting from the multidimensional approach to screening. Crystal structure analysis and substrate docking show how the enzyme active site changes over the course of the evolutions as either a direct or an indirect result of mutations.

-	~~The entry 4u2e is ON HOLD until Paper Publication~~	+	Directed Evolution of New and Improved Enzyme Functions Using an Evolutionary Intermediate and Multidirectional Search.,Porter JL, Boon PL, Murray TP, Huber T, Collyer CA, Ollis DL ACS Chem Biol. 2014 Dec 5. PMID:25419863<ref>PMID:25419863</ref>

-	~~Authors: Porter~~, J.L., Collyer, C.A., Ollis, D.L.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~:	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Carboxymethylenebutenolidase]]
		+	[[Category: Collyer, C A]]
		+	[[Category: Ollis, D L]]
		+	[[Category: Porter, J L]]
		+	[[Category: A/b hydrolase fold]]
		+	[[Category: Hydrolase]]

OCA at 07:14, 30 July 2014

OCA — Wed, 30 Jul 2014 07:14:44 GMT

←Older revision		Revision as of 07:14, 30 July 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4u2e is ON HOLD	+	The entry 4u2e is ON HOLD until Paper Publication

	Authors: Porter, J.L., Collyer, C.A., Ollis, D.L.		Authors: Porter, J.L., Collyer, C.A., Ollis, D.L.

	Description:		Description:

OCA: Protected "4u2e" [edit=sysop:move=sysop]

OCA — Wed, 23 Jul 2014 07:26:39 GMT

Protected "4u2e" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:26, 23 July 2014

OCA: New page: '''Unreleased structure''' The entry 4u2e is ON HOLD Authors: Porter, J.L., Collyer, C.A., Ollis, D.L. Description:

OCA — Wed, 23 Jul 2014 07:26:38 GMT

New page: '''Unreleased structure''' The entry 4u2e is ON HOLD Authors: Porter, J.L., Collyer, C.A., Ollis, D.L. Description:

New page

'''Unreleased structure'''

The entry 4u2e is ON HOLD

Authors: Porter, J.L., Collyer, C.A., Ollis, D.L.

Description: