4u2y - Revision history

OCA at 00:49, 28 December 2023

OCA — Thu, 28 Dec 2023 00:49:44 GMT

←Older revision		Revision as of 00:49, 28 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4u2y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U2Y FirstGlance]. <br>		<table><tr><td colspan='2'>[[4u2y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U2Y FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RZM:(2R,3R,4R,5R)-4-HYDROXY-2,5-BIS(HYDROXYMETHYL)PYRROLIDIN-3-YL+ALPHA-D-GLUCOPYRANOSIDE'>RZM</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.483Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RZM:(2R,3R,4R,5R)-4-HYDROXY-2,5-BIS(HYDROXYMETHYL)PYRROLIDIN-3-YL+ALPHA-D-GLUCOPYRANOSIDE'>RZM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2y OCA], [https://pdbe.org/4u2y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u2y RCSB], [https://www.ebi.ac.uk/pdbsum/4u2y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u2y ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2y OCA], [https://pdbe.org/4u2y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u2y RCSB], [https://www.ebi.ac.uk/pdbsum/4u2y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u2y ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:58, 22 March 2023

OCA — Wed, 22 Mar 2023 07:58:58 GMT

←Older revision		Revision as of 07:58, 22 March 2023
Line 3:		Line 3:
	<StructureSection load='4u2y' size='340' side='right'caption='[[4u2y]], [[Resolution\|resolution]] 2.48Å' scene=''>		<StructureSection load='4u2y' size='340' side='right'caption='[[4u2y]], [[Resolution\|resolution]] 2.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4u2y]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Strco Strco~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2Y OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4U2Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4u2y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U2Y FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RZM:(2R,3R,4R,5R)-4-HYDROXY-2,5-BIS(HYDROXYMETHYL)PYRROLIDIN-3-YL+ALPHA-D-GLUCOPYRANOSIDE'>RZM</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RZM:(2R,3R,4R,5R)-4-HYDROXY-2,5-BIS(HYDROXYMETHYL)PYRROLIDIN-3-YL+ALPHA-D-GLUCOPYRANOSIDE'>RZM</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4u2z\|4u2z]], [[4u31\|4u31]], [[4u33\|4u33]], [[4u3c\|4u3c]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2y OCA], [https://pdbe.org/4u2y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u2y RCSB], [https://www.ebi.ac.uk/pdbsum/4u2y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u2y ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">glgE1, pep1, pep1A, pep1I, SCO5443, SC6A11.19c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Starch_synthase_(maltosyl-transferring) Starch synthase (maltosyl-transferring)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.99.16 2.4.99.16] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4u2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2y OCA], [~~http~~://pdbe.org/4u2y PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4u2y RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4u2y PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4u2y ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GLGE1_STRCO GLGE1_STRCO]] Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)-glucans. Maltooligosaccharides with a degree of polymerization (DP) superior or equal to 4 are efficient acceptors, with DP6 being optimal in the GlgE-catalyzed polymerization with M1P. Is specific for the alpha-anomer of M1P as substrate, since the beta-anomer of M1P gives no activity. Alpha-D-glucose 1-phosphate cannot serve as a donor substrate, but alpha-maltosyl fluoride is an efficient donor in vitro. Exhibits an alpha-retaining catalytic mechanism, with evidence that maltooligosaccharide acceptors are extended at their non-reducing ends. Is also able to catalyze the reverse reaction in vitro, releasing M1P from glycogen or maltoheptaose in the presence of inorganic phosphate. Also catalyzes disproportionation reactions through maltosyl transfer between maltooligosaccharides. Is probably involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB.<ref>PMID:21914799</ref>	+	[https://www.uniprot.org/uniprot/GLGE1_STRCO GLGE1_STRCO] Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)-glucans. Maltooligosaccharides with a degree of polymerization (DP) superior or equal to 4 are efficient acceptors, with DP6 being optimal in the GlgE-catalyzed polymerization with M1P. Is specific for the alpha-anomer of M1P as substrate, since the beta-anomer of M1P gives no activity. Alpha-D-glucose 1-phosphate cannot serve as a donor substrate, but alpha-maltosyl fluoride is an efficient donor in vitro. Exhibits an alpha-retaining catalytic mechanism, with evidence that maltooligosaccharide acceptors are extended at their non-reducing ends. Is also able to catalyze the reverse reaction in vitro, releasing M1P from glycogen or maltoheptaose in the presence of inorganic phosphate. Also catalyzes disproportionation reactions through maltosyl transfer between maltooligosaccharides. Is probably involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB.<ref>PMID:21914799</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Strco]]~~	+	[[Category: Lindenberger JJ]]
-	[[Category: Lindenberger~~, J J~~]]	+	[[Category: Ronning DR]]
-	[[Category: Ronning~~, D R]]~~	+
-	~~[[Category: Inihibitor complex azasugar maltosyl transferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 15:39, 11 December 2019

OCA — Wed, 11 Dec 2019 15:39:07 GMT

←Older revision		Revision as of 15:39, 11 December 2019
Line 1:		Line 1:

	==Sco GlgEI-V279S in Complex with Reaction Intermediate Azasugar==		==Sco GlgEI-V279S in Complex with Reaction Intermediate Azasugar==
-	<StructureSection load='4u2y' size='340' side='right' caption='[[4u2y]], [[Resolution\|resolution]] 2.48Å' scene=''>	+	<StructureSection load='4u2y' size='340' side='right'caption='[[4u2y]], [[Resolution\|resolution]] 2.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4u2y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U2Y FirstGlance]. <br>		<table><tr><td colspan='2'>[[4u2y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U2Y FirstGlance]. <br>
Line 25:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Strco]]		[[Category: Strco]]
	[[Category: Lindenberger, J J]]		[[Category: Lindenberger, J J]]

OCA at 15:04, 15 November 2017

OCA — Wed, 15 Nov 2017 15:04:35 GMT

←Older revision		Revision as of 15:04, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='4u2y' size='340' side='right' caption='[[4u2y]], [[Resolution\|resolution]] 2.48Å' scene=''>		<StructureSection load='4u2y' size='340' side='right' caption='[[4u2y]], [[Resolution\|resolution]] 2.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4u2y]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U2Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4u2y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U2Y FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RZM:(2R,3R,4R,5R)-4-HYDROXY-2,5-BIS(HYDROXYMETHYL)PYRROLIDIN-3-YL+ALPHA-D-GLUCOPYRANOSIDE'>RZM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RZM:(2R,3R,4R,5R)-4-HYDROXY-2,5-BIS(HYDROXYMETHYL)PYRROLIDIN-3-YL+ALPHA-D-GLUCOPYRANOSIDE'>RZM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4u2z\|4u2z]], [[4u31\|4u31]], [[4u33\|4u33]], [[4u3c\|4u3c]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4u2z\|4u2z]], [[4u31\|4u31]], [[4u33\|4u33]], [[4u3c\|4u3c]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">glgE1, pep1, pep1A, pep1I, SCO5443, SC6A11.19c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Starch_synthase_(maltosyl-transferring) Starch synthase (maltosyl-transferring)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.99.16 2.4.99.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Starch_synthase_(maltosyl-transferring) Starch synthase (maltosyl-transferring)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.99.16 2.4.99.16] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2y OCA], [http://pdbe.org/4u2y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4u2y RCSB], [http://www.ebi.ac.uk/pdbsum/4u2y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4u2y ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2y OCA], [http://pdbe.org/4u2y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4u2y RCSB], [http://www.ebi.ac.uk/pdbsum/4u2y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4u2y ProSAT]</span></td></tr>
Line 24:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Strco]]
	[[Category: Lindenberger, J J]]		[[Category: Lindenberger, J J]]
	[[Category: Ronning, D R]]		[[Category: Ronning, D R]]
	[[Category: Inihibitor complex azasugar maltosyl transferase]]		[[Category: Inihibitor complex azasugar maltosyl transferase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 14:12, 24 May 2017

OCA — Wed, 24 May 2017 14:12:14 GMT

←Older revision		Revision as of 14:12, 24 May 2017
Line 1:		Line 1:
		+
	==Sco GlgEI-V279S in Complex with Reaction Intermediate Azasugar==		==Sco GlgEI-V279S in Complex with Reaction Intermediate Azasugar==
	<StructureSection load='4u2y' size='340' side='right' caption='[[4u2y]], [[Resolution\|resolution]] 2.48Å' scene=''>		<StructureSection load='4u2y' size='340' side='right' caption='[[4u2y]], [[Resolution\|resolution]] 2.48Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4u2z\|4u2z]], [[4u31\|4u31]], [[4u33\|4u33]], [[4u3c\|4u3c]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4u2z\|4u2z]], [[4u31\|4u31]], [[4u33\|4u33]], [[4u3c\|4u3c]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Starch_synthase_(maltosyl-transferring) Starch synthase (maltosyl-transferring)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.99.16 2.4.99.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Starch_synthase_(maltosyl-transferring) Starch synthase (maltosyl-transferring)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.99.16 2.4.99.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u2y RCSB], [http://www.ebi.ac.uk/pdbsum/4u2y PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2y OCA], [http://pdbe.org/4u2y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4u2y RCSB], [http://www.ebi.ac.uk/pdbsum/4u2y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4u2y ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4u2y" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:15, 20 August 2015

OCA — Thu, 20 Aug 2015 06:15:12 GMT

←Older revision		Revision as of 06:15, 20 August 2015
Line 10:		Line 10:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/GLGE1_STRCO GLGE1_STRCO]] Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)-glucans. Maltooligosaccharides with a degree of polymerization (DP) superior or equal to 4 are efficient acceptors, with DP6 being optimal in the GlgE-catalyzed polymerization with M1P. Is specific for the alpha-anomer of M1P as substrate, since the beta-anomer of M1P gives no activity. Alpha-D-glucose 1-phosphate cannot serve as a donor substrate, but alpha-maltosyl fluoride is an efficient donor in vitro. Exhibits an alpha-retaining catalytic mechanism, with evidence that maltooligosaccharide acceptors are extended at their non-reducing ends. Is also able to catalyze the reverse reaction in vitro, releasing M1P from glycogen or maltoheptaose in the presence of inorganic phosphate. Also catalyzes disproportionation reactions through maltosyl transfer between maltooligosaccharides. Is probably involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB.<ref>PMID:21914799</ref>		[[http://www.uniprot.org/uniprot/GLGE1_STRCO GLGE1_STRCO]] Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)-glucans. Maltooligosaccharides with a degree of polymerization (DP) superior or equal to 4 are efficient acceptors, with DP6 being optimal in the GlgE-catalyzed polymerization with M1P. Is specific for the alpha-anomer of M1P as substrate, since the beta-anomer of M1P gives no activity. Alpha-D-glucose 1-phosphate cannot serve as a donor substrate, but alpha-maltosyl fluoride is an efficient donor in vitro. Exhibits an alpha-retaining catalytic mechanism, with evidence that maltooligosaccharide acceptors are extended at their non-reducing ends. Is also able to catalyze the reverse reaction in vitro, releasing M1P from glycogen or maltoheptaose in the presence of inorganic phosphate. Also catalyzes disproportionation reactions through maltosyl transfer between maltooligosaccharides. Is probably involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB.<ref>PMID:21914799</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	GlgE is a bacterial maltosyltransferase that catalyzes the elongation of a cytosolic, branched alpha-glucan. In Mycobacterium tuberculosis (M. tb), inactivation of GlgE (Mtb GlgE) results in the rapid death of the organism due to a toxic accumulation of the maltosyl donor, maltose-1-phosphate (M1P), suggesting that GlgE is an intriguing target for inhibitor design. In this study, the crystal structures of the Mtb GlgE in a binary complex with maltose and a ternary complex with maltose and a maltosyl-acceptor molecule, maltohexaose, were solved to 3.3 A and 4.0 A, respectively. The maltohexaose structure reveals a dominant site for alpha-glucan binding. To obtain more detailed interactions between first generation, non-covalent inhibitors and GlgE, a variant Streptomyces coelicolor GlgEI (Sco GlgEI-V279S) was made to better emulate the Mtb GlgE M1P binding site. The structure of Sco GlgEI-V279S complexed with alpha-maltose-C-phosphonate (MCP), a non-hydrolyzable substrate analogue, was solved to 1.9 A resolution, and the structure of Sco GlgEI-V279S complexed with 2,5-dideoxy-3-O-alpha-D-glucopyranosyl-2,5-imino-D-mannitol (DDGIM), an oxocarbenium mimic, was solved to 2.5 A resolution. These structures detail important interactions that contribute to the inhibitory activity of these compounds, and provide information on future designs that may be exploited to improve upon these first generation GlgE inhibitors.
		+
		+	Crystal structures of Mycobacterium tuberculosis GlgE and complexes with non-covalent inhibitors.,Lindenberger JJ, Kumar Veleti S, Wilson BN, Sucheck SJ, Ronning DR Sci Rep. 2015 Aug 6;5:12830. doi: 10.1038/srep12830. PMID:26245983<ref>PMID:26245983</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:47, 12 August 2015

OCA — Wed, 12 Aug 2015 14:47:14 GMT

←Older revision		Revision as of 14:47, 12 August 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Sco GlgEI-V279S in Complex with Reaction Intermediate Azasugar==
-		+	<StructureSection load='4u2y' size='340' side='right' caption='[[4u2y]], [[Resolution\|resolution]] 2.48Å' scene=''>
-	~~The entry~~ 4u2y is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4u2y]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U2Y FirstGlance]. <br>
-	~~Authors~~: ~~Ronning~~, D.R., ~~Lindenberger~~, J.J.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RZM:(2R,3R,4R,5R)-4-HYDROXY-2,5-BIS(HYDROXYMETHYL)PYRROLIDIN-3-YL+ALPHA-D-GLUCOPYRANOSIDE'>RZM</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4u2z\|4u2z]], [[4u31\|4u31]], [[4u33\|4u33]], [[4u3c\|4u3c]]</td></tr>
-	~~Description~~:	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Starch_synthase_(maltosyl-transferring) Starch synthase (maltosyl-transferring)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.99.16 2.4.99.16] </span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u2y RCSB], [http://www.ebi.ac.uk/pdbsum/4u2y PDBsum]</span></td></tr>
-	[[Category: Ronning, D.R]]	+	</table>
-	[[Category: ~~Lindenberger, J.J~~]]	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GLGE1_STRCO GLGE1_STRCO]] Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)-glucans. Maltooligosaccharides with a degree of polymerization (DP) superior or equal to 4 are efficient acceptors, with DP6 being optimal in the GlgE-catalyzed polymerization with M1P. Is specific for the alpha-anomer of M1P as substrate, since the beta-anomer of M1P gives no activity. Alpha-D-glucose 1-phosphate cannot serve as a donor substrate, but alpha-maltosyl fluoride is an efficient donor in vitro. Exhibits an alpha-retaining catalytic mechanism, with evidence that maltooligosaccharide acceptors are extended at their non-reducing ends. Is also able to catalyze the reverse reaction in vitro, releasing M1P from glycogen or maltoheptaose in the presence of inorganic phosphate. Also catalyzes disproportionation reactions through maltosyl transfer between maltooligosaccharides. Is probably involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB.<ref>PMID:21914799</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Lindenberger, J J]]
		+	[[Category: Ronning, D R]]
		+	[[Category: Inihibitor complex azasugar maltosyl transferase]]
		+	[[Category: Transferase]]

OCA at 18:04, 24 December 2014

OCA — Wed, 24 Dec 2014 18:04:39 GMT

←Older revision		Revision as of 18:04, 24 December 2014
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	Authors: Ronning, D.R., Lindenberger, J.J.		Authors: Ronning, D.R., Lindenberger, J.J.

-	Description:	+	Description:
		+	[[Category: Unreleased Structures]]
		+	[[Category: Ronning, D.R]]
		+	[[Category: Lindenberger, J.J]]

OCA at 11:39, 19 November 2014

OCA — Wed, 19 Nov 2014 11:39:03 GMT

←Older revision		Revision as of 11:39, 19 November 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4u2y is ON HOLD	+	The entry 4u2y is ON HOLD until Paper Publication

	Authors: Ronning, D.R., Lindenberger, J.J.		Authors: Ronning, D.R., Lindenberger, J.J.

	Description:		Description:

OCA at 01:28, 7 August 2014

OCA — Thu, 07 Aug 2014 01:28:33 GMT

←Older revision		Revision as of 01:28, 7 August 2014
Line 3:		Line 3:
	The entry 4u2y is ON HOLD		The entry 4u2y is ON HOLD

-	Authors: ~~Lindenberger~~, J.L., ~~Ronning~~, D.R.	+	Authors: Ronning, D.R., Lindenberger, J.J.

	Description:		Description: