4u5v - Revision history

OCA at 11:19, 9 May 2024

OCA — Thu, 09 May 2024 11:19:22 GMT

←Older revision		Revision as of 11:19, 9 May 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4u5v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U5V FirstGlance]. <br>		<table><tr><td colspan='2'>[[4u5v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U5V FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RH2:N~2~-{[5-(PYRIDIN-3-YL)THIOPHEN-2-YL]METHYL}-L-LYSINAMIDE'>RH2</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.968Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RH2:N~2~-{[5-(PYRIDIN-3-YL)THIOPHEN-2-YL]METHYL}-L-LYSINAMIDE'>RH2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u5v OCA], [https://pdbe.org/4u5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u5v RCSB], [https://www.ebi.ac.uk/pdbsum/4u5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u5v ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u5v OCA], [https://pdbe.org/4u5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u5v RCSB], [https://www.ebi.ac.uk/pdbsum/4u5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u5v ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:04, 22 March 2023

OCA — Wed, 22 Mar 2023 08:04:14 GMT

←Older revision		Revision as of 08:04, 22 March 2023
Line 3:		Line 3:
	<StructureSection load='4u5v' size='340' side='right'caption='[[4u5v]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='4u5v' size='340' side='right'caption='[[4u5v]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4u5v]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U5V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4U5V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4u5v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U5V FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RH2:N~2~-{[5-(PYRIDIN-3-YL)THIOPHEN-2-YL]METHYL}-L-LYSINAMIDE'>RH2</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RH2:N~2~-{[5-(PYRIDIN-3-YL)THIOPHEN-2-YL]METHYL}-L-LYSINAMIDE'>RH2</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Kpna2, Rch1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u5v OCA], [https://pdbe.org/4u5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u5v RCSB], [https://www.ebi.ac.uk/pdbsum/4u5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u5v ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4u5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u5v OCA], [~~http~~://pdbe.org/4u5v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4u5v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4u5v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4u5v ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/IMA1_MOUSE IMA1_MOUSE]] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.	+	[https://www.uniprot.org/uniprot/IMA1_MOUSE IMA1_MOUSE] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4u5v" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4u5v" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Importin 3D structures\|Importin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Holvey~~, R S~~]]	+	[[Category: Holvey RS]]
-	[[Category: Stewart, M]]	+	[[Category: Stewart M]]
-	[[Category: Valkov, E]]	+	[[Category: Valkov E]]
-	~~[[Category: Importin-alpha]]~~	+
-	~~[[Category: Tpx2]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 14:30, 10 May 2019

OCA — Fri, 10 May 2019 14:30:28 GMT

←Older revision		Revision as of 14:30, 10 May 2019
Line 1:		Line 1:

	==IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR==		==IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR==
-	<StructureSection load='4u5v' size='340' side='right' caption='[[4u5v]], [[Resolution\|resolution]] 1.97Å' scene=''>	+	<StructureSection load='4u5v' size='340' side='right'caption='[[4u5v]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4u5v]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U5V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4u5v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U5V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RH2:N~2~-{[5-(PYRIDIN-3-YL)THIOPHEN-2-YL]METHYL}-L-LYSINAMIDE'>RH2</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RH2:N~2~-{[5-(PYRIDIN-3-YL)THIOPHEN-2-YL]METHYL}-L-LYSINAMIDE'>RH2</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Kpna2, Rch1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u5v OCA], [http://pdbe.org/4u5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4u5v RCSB], [http://www.ebi.ac.uk/pdbsum/4u5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4u5v ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u5v OCA], [http://pdbe.org/4u5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4u5v RCSB], [http://www.ebi.ac.uk/pdbsum/4u5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4u5v ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Holvey, R S]]		[[Category: Holvey, R S]]
	[[Category: Stewart, M]]		[[Category: Stewart, M]]

OCA at 12:11, 12 April 2017

OCA — Wed, 12 Apr 2017 12:11:06 GMT

←Older revision		Revision as of 12:11, 12 April 2017
Line 1:		Line 1:
		+
	==IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR==		==IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR==
	<StructureSection load='4u5v' size='340' side='right' caption='[[4u5v]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='4u5v' size='340' side='right' caption='[[4u5v]], [[Resolution\|resolution]] 1.97Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4u5v]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U5V FirstGlance]. <br>		<table><tr><td colspan='2'>[[4u5v]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U5V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RH2:N~2~-{[5-(PYRIDIN-3-YL)THIOPHEN-2-YL]METHYL}-L-LYSINAMIDE'>RH2</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RH2:N~2~-{[5-(PYRIDIN-3-YL)THIOPHEN-2-YL]METHYL}-L-LYSINAMIDE'>RH2</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u5v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u5v RCSB], [http://www.ebi.ac.uk/pdbsum/4u5v PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u5v OCA], [http://pdbe.org/4u5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4u5v RCSB], [http://www.ebi.ac.uk/pdbsum/4u5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4u5v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4u5v" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:17, 13 May 2015

OCA — Wed, 13 May 2015 12:17:38 GMT

←Older revision		Revision as of 12:17, 13 May 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR==
		+	<StructureSection load='4u5v' size='340' side='right' caption='[[4u5v]], [[Resolution\|resolution]] 1.97Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4u5v]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U5V FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RH2:N~2~-{[5-(PYRIDIN-3-YL)THIOPHEN-2-YL]METHYL}-L-LYSINAMIDE'>RH2</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u5v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u5v RCSB], [http://www.ebi.ac.uk/pdbsum/4u5v PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IMA1_MOUSE IMA1_MOUSE]] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein-protein interactions are difficult therapeutic targets, and inhibiting pathologically relevant interactions without disrupting other essential ones presents an additional challenge. Herein we report how this might be achieved for the potential anticancer target, the TPX2-importin-alpha interaction. Importin-alpha is a nuclear transport protein that regulates the spindle assembly protein TPX2. It has two binding sites-major and minor-to which partners bind. Most nuclear transport cargoes use the major site, whereas TPX2 binds principally to the minor site. Fragment-based approaches were used to identify small molecules that bind importin-alpha, and crystallographic studies identified a lead series that was observed to bind specifically to the minor site, representing the first ligands specific for this site. Structure-guided synthesis informed the elaboration of these fragments to explore the source of ligand selectivity between the minor and major sites. These ligands are starting points for the development of inhibitors of this protein-protein interaction.

-	~~The entry 4u5v is ON HOLD~~	+	Selective Targeting of the TPX2 Site of Importin-alpha Using Fragment-Based Ligand Design.,Holvey RS, Valkov E, Neal D, Stewart M, Abell C ChemMedChem. 2015 Apr 20. doi: 10.1002/cmdc.201500014. PMID:25899172<ref>PMID:25899172</ref>

-	~~Authors: Stewart~~, M.~~, Valkov, E~~.~~, Holvey, R.S~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: ~~Holvey~~, ~~R.S~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Holvey, R S]]
		+	[[Category: Stewart, M]]
	[[Category: Valkov, E]]		[[Category: Valkov, E]]
-	[[Category: ~~Stewart, M~~]]	+	[[Category: Importin-alpha]]
		+	[[Category: Tpx2]]
		+	[[Category: Transport protein]]

OCA at 18:10, 24 December 2014

OCA — Wed, 24 Dec 2014 18:10:10 GMT

←Older revision		Revision as of 18:10, 24 December 2014
Line 6:		Line 6:

	Description: IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR		Description: IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR
		+	[[Category: Unreleased Structures]]
		+	[[Category: Holvey, R.S]]
		+	[[Category: Valkov, E]]
		+	[[Category: Stewart, M]]

OCA: Protected "4u5v" [edit=sysop:move=sysop]

OCA — Thu, 07 Aug 2014 01:31:19 GMT

Protected "4u5v" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:31, 7 August 2014

OCA: New page: '''Unreleased structure''' The entry 4u5v is ON HOLD Authors: Stewart, M., Valkov, E., Holvey, R.S. Description: IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR

OCA — Thu, 07 Aug 2014 01:31:18 GMT

New page: '''Unreleased structure''' The entry 4u5v is ON HOLD Authors: Stewart, M., Valkov, E., Holvey, R.S. Description: IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR

New page

'''Unreleased structure'''

The entry 4u5v is ON HOLD

Authors: Stewart, M., Valkov, E., Holvey, R.S.

Description: IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR