4u77 - Revision history

OCA at 11:19, 9 May 2024

OCA — Thu, 09 May 2024 11:19:23 GMT

←Older revision		Revision as of 11:19, 9 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4u77]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U77 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4u77]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U77 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.03Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u77 OCA], [https://pdbe.org/4u77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u77 RCSB], [https://www.ebi.ac.uk/pdbsum/4u77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u77 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u77 OCA], [https://pdbe.org/4u77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u77 RCSB], [https://www.ebi.ac.uk/pdbsum/4u77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u77 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:06, 22 March 2023

OCA — Wed, 22 Mar 2023 08:06:13 GMT

←Older revision		Revision as of 08:06, 22 March 2023
Line 3:		Line 3:
	<StructureSection load='4u77' size='340' side='right'caption='[[4u77]], [[Resolution\|resolution]] 2.03Å' scene=''>		<StructureSection load='4u77' size='340' side='right'caption='[[4u77]], [[Resolution\|resolution]] 2.03Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4u77]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Drome Drome~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U77 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4U77 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4u77]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U77 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cp190, E(mod)4-1, CG6384 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u77 OCA], [https://pdbe.org/4u77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u77 RCSB], [https://www.ebi.ac.uk/pdbsum/4u77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u77 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4u77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u77 OCA], [~~http~~://pdbe.org/4u77 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4u77 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4u77 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4u77 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CP190_DROME CP190_DROME]] Component of the gypsy chromatin insulator complex which is required for the function of the gypsy chromatin insulator and other endogenous chromatin insulators. Chromatin insulators are regulatory elements which establish independent domains of transcriptional activity within eukaryotic genomes. Insulators have two defining properties; they can block the communication between an enhancer and a promoter when placed between them and can also buffer transgenes from position effect variegation (PEV). Insulators are proposed to structure the chromatin fiber into independent domains of differing transcriptional potential by promoting the formation of distinct chromatin loops. This chromatin looping may involve the formation of insulator bodies, where homotypic interactions between individual subunits of the insulator complex could promote the clustering of widely spaced insulators at the nuclear periphery. Within the gypsy insulator complex, this protein may directly bind to insulator DNA at sites distinct from those recognized by su(Hw). Required during embryogenesis for axial expansion, an actin/myosin dependent process that distributes the dividing nuclei along the anterior-posterior axis of the syncytial embryo. Does not appear to play a crucial role in organizing centrosomal microtubules during mitosis.<ref>PMID:14996941</ref> <ref>PMID:15574329</ref> <ref>PMID:16051175</ref>	+	[https://www.uniprot.org/uniprot/CP190_DROME CP190_DROME] Component of the gypsy chromatin insulator complex which is required for the function of the gypsy chromatin insulator and other endogenous chromatin insulators. Chromatin insulators are regulatory elements which establish independent domains of transcriptional activity within eukaryotic genomes. Insulators have two defining properties; they can block the communication between an enhancer and a promoter when placed between them and can also buffer transgenes from position effect variegation (PEV). Insulators are proposed to structure the chromatin fiber into independent domains of differing transcriptional potential by promoting the formation of distinct chromatin loops. This chromatin looping may involve the formation of insulator bodies, where homotypic interactions between individual subunits of the insulator complex could promote the clustering of widely spaced insulators at the nuclear periphery. Within the gypsy insulator complex, this protein may directly bind to insulator DNA at sites distinct from those recognized by su(Hw). Required during embryogenesis for axial expansion, an actin/myosin dependent process that distributes the dividing nuclei along the anterior-posterior axis of the syncytial embryo. Does not appear to play a crucial role in organizing centrosomal microtubules during mitosis.<ref>PMID:14996941</ref> <ref>PMID:15574329</ref> <ref>PMID:16051175</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Drome~~]]	+	[[Category: Drosophila melanogaster]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Allemand, F]]	+	[[Category: Allemand F]]
-	[[Category: Cohen-Gonsaud, M]]	+	[[Category: Cohen-Gonsaud M]]
-	[[Category: Labesse, G]]	+	[[Category: Labesse G]]
-	[[Category: Nollmann, M]]	+	[[Category: Nollmann M]]
-	~~[[Category: Btb fold]]~~	+
-	~~[[Category: Dimer]]~~	+
-	~~[[Category: Peptide binding groove]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 14:57, 10 May 2019

OCA — Fri, 10 May 2019 14:57:20 GMT

←Older revision		Revision as of 14:57, 10 May 2019
Line 1:		Line 1:

	==BTB domain from Drosophila CP190==		==BTB domain from Drosophila CP190==
-	<StructureSection load='4u77' size='340' side='right' caption='[[4u77]], [[Resolution\|resolution]] 2.03Å' scene=''>	+	<StructureSection load='4u77' size='340' side='right'caption='[[4u77]], [[Resolution\|resolution]] 2.03Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4u77]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U77 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4u77]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U77 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cp190, E(mod)4-1, CG6384 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u77 OCA], [http://pdbe.org/4u77 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4u77 RCSB], [http://www.ebi.ac.uk/pdbsum/4u77 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4u77 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u77 OCA], [http://pdbe.org/4u77 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4u77 RCSB], [http://www.ebi.ac.uk/pdbsum/4u77 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4u77 ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Drome]]
		+	[[Category: Large Structures]]
	[[Category: Allemand, F]]		[[Category: Allemand, F]]
	[[Category: Cohen-Gonsaud, M]]		[[Category: Cohen-Gonsaud, M]]

OCA at 16:34, 4 August 2016

OCA — Thu, 04 Aug 2016 16:34:13 GMT

←Older revision		Revision as of 16:34, 4 August 2016
Line 1:		Line 1:
		+
	==BTB domain from Drosophila CP190==		==BTB domain from Drosophila CP190==
	<StructureSection load='4u77' size='340' side='right' caption='[[4u77]], [[Resolution\|resolution]] 2.03Å' scene=''>		<StructureSection load='4u77' size='340' side='right' caption='[[4u77]], [[Resolution\|resolution]] 2.03Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4u77]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U77 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4u77]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U77 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u77 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u77 RCSB], [http://www.ebi.ac.uk/pdbsum/4u77 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u77 OCA], [http://pdbe.org/4u77 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4u77 RCSB], [http://www.ebi.ac.uk/pdbsum/4u77 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4u77 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4u77" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:01, 25 December 2014

OCA — Thu, 25 Dec 2014 11:01:53 GMT

←Older revision		Revision as of 11:01, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u77 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u77 RCSB], [http://www.ebi.ac.uk/pdbsum/4u77 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u77 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u77 RCSB], [http://www.ebi.ac.uk/pdbsum/4u77 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CP190_DROME CP190_DROME]] Component of the gypsy chromatin insulator complex which is required for the function of the gypsy chromatin insulator and other endogenous chromatin insulators. Chromatin insulators are regulatory elements which establish independent domains of transcriptional activity within eukaryotic genomes. Insulators have two defining properties; they can block the communication between an enhancer and a promoter when placed between them and can also buffer transgenes from position effect variegation (PEV). Insulators are proposed to structure the chromatin fiber into independent domains of differing transcriptional potential by promoting the formation of distinct chromatin loops. This chromatin looping may involve the formation of insulator bodies, where homotypic interactions between individual subunits of the insulator complex could promote the clustering of widely spaced insulators at the nuclear periphery. Within the gypsy insulator complex, this protein may directly bind to insulator DNA at sites distinct from those recognized by su(Hw). Required during embryogenesis for axial expansion, an actin/myosin dependent process that distributes the dividing nuclei along the anterior-posterior axis of the syncytial embryo. Does not appear to play a crucial role in organizing centrosomal microtubules during mitosis.<ref>PMID:14996941</ref> <ref>PMID:15574329</ref> <ref>PMID:16051175</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Allemand, F.]]	+	[[Category: Allemand, F]]
-	[[Category: Cohen-Gonsaud, M.]]	+	[[Category: Cohen-Gonsaud, M]]
-	[[Category: Labesse, G.]]	+	[[Category: Labesse, G]]
-	[[Category: Nollmann, M.]]	+	[[Category: Nollmann, M]]
	[[Category: Btb fold]]		[[Category: Btb fold]]
	[[Category: Dimer]]		[[Category: Dimer]]
	[[Category: Peptide binding groove]]		[[Category: Peptide binding groove]]
	[[Category: Transcription]]		[[Category: Transcription]]

OCA at 08:28, 20 October 2014

OCA — Mon, 20 Oct 2014 08:28:38 GMT

←Older revision		Revision as of 08:28, 20 October 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4u77]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U77 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4u77]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U77 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u77 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u77 RCSB], [http://www.ebi.ac.uk/pdbsum/4u77 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u77 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u77 RCSB], [http://www.ebi.ac.uk/pdbsum/4u77 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:11, 10 September 2014

OCA — Wed, 10 Sep 2014 07:11:24 GMT

←Older revision		Revision as of 07:11, 10 September 2014
Line 6:		Line 6:
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u77 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u77 RCSB], [http://www.ebi.ac.uk/pdbsum/4u77 PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u77 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u77 RCSB], [http://www.ebi.ac.uk/pdbsum/4u77 PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chromatin insulators are genetic elements implicated in the organization of chromatin and the regulation of transcription. In Drosophila, different insulator types were characterized by their locus-specific composition of insulator proteins and co-factors. Insulators mediate specific long-range DNA contacts required for the three dimensional organization of the interphase nucleus and for transcription regulation, but the mechanisms underlying the formation of these contacts is currently unknown. Here, we investigate the molecular associations between different components of insulator complexes (BEAF32, CP190 and Chromator) by biochemical and biophysical means, and develop a novel single-molecule assay to determine what factors are necessary and essential for the formation of long-range DNA interactions. We show that BEAF32 is able to bind DNA specifically and with high affinity, but not to bridge long-range interactions (LRI). In contrast, we show that CP190 and Chromator are able to mediate LRI between specifically-bound BEAF32 nucleoprotein complexes in vitro. This ability of CP190 and Chromator to establish LRI requires specific contacts between BEAF32 and their C-terminal domains, and dimerization through their N-terminal domains. In particular, the BTB/POZ domains of CP190 form a strict homodimer, and its C-terminal domain interacts with several insulator binding proteins. We propose a general model for insulator function in which BEAF32/dCTCF/Su(HW) provide DNA specificity (first layer proteins) whereas CP190/Chromator are responsible for the physical interactions required for long-range contacts (second layer). This network of organized, multi-layer interactions could explain the different activities of insulators as chromatin barriers, enhancer blockers, and transcriptional regulators, and suggest a general mechanism for how insulators may shape the organization of higher-order chromatin during cell division.
		+
		+	Chromatin insulator factors involved in long-range DNA interactions and their role in the folding of the Drosophila genome.,Vogelmann J, Le Gall A, Dejardin S, Allemand F, Gamot A, Labesse G, Cuvier O, Negre N, Cohen-Gonsaud M, Margeat E, Nollmann M PLoS Genet. 2014 Aug 28;10(8):e1004544. doi: 10.1371/journal.pgen.1004544., eCollection 2014 Aug. PMID:25165871<ref>PMID:25165871</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 09:21, 13 August 2014

OCA — Wed, 13 Aug 2014 09:21:44 GMT

←Older revision		Revision as of 09:21, 13 August 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==BTB domain from Drosophila CP190==
-		+	<StructureSection load='4u77' size='340' side='right' caption='[[4u77]], [[Resolution\|resolution]] 2.03Å' scene=''>
-	~~The entry~~ 4u77 is ~~ON HOLD~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4u77]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U77 FirstGlance]. <br>
-	~~Authors~~: Allemand, F., Cohen-Gonsaud, M., Labesse, G., Nollmann, M.	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>
-		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u77 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u77 RCSB], [http://www.ebi.ac.uk/pdbsum/4u77 PDBsum]</span></td></tr>
-	~~Description~~: ~~BTB domain from Drosophila CP190~~	+	<table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Allemand, F.]]
		+	[[Category: Cohen-Gonsaud, M.]]
		+	[[Category: Labesse, G.]]
		+	[[Category: Nollmann, M.]]
		+	[[Category: Btb fold]]
		+	[[Category: Dimer]]
		+	[[Category: Peptide binding groove]]
		+	[[Category: Transcription]]

OCA: Protected "4u77" [edit=sysop:move=sysop]

OCA — Thu, 07 Aug 2014 01:33:04 GMT

Protected "4u77" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:33, 7 August 2014

OCA: New page: '''Unreleased structure''' The entry 4u77 is ON HOLD Authors: Allemand, F., Cohen-Gonsaud, M., Labesse, G., Nollmann, M. Description: BTB domain from Drosophila CP190

OCA — Thu, 07 Aug 2014 01:33:02 GMT

New page: '''Unreleased structure''' The entry 4u77 is ON HOLD Authors: Allemand, F., Cohen-Gonsaud, M., Labesse, G., Nollmann, M. Description: BTB domain from Drosophila CP190

New page

'''Unreleased structure'''

The entry 4u77 is ON HOLD

Authors: Allemand, F., Cohen-Gonsaud, M., Labesse, G., Nollmann, M.

Description: BTB domain from Drosophila CP190