4ub0 - Revision history

OCA at 00:50, 28 December 2023

OCA — Thu, 28 Dec 2023 00:50:49 GMT

←Older revision		Revision as of 00:50, 28 December 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ub0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UB0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ub0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UB0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ub0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ub0 OCA], [https://pdbe.org/4ub0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ub0 RCSB], [https://www.ebi.ac.uk/pdbsum/4ub0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ub0 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ub0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ub0 OCA], [https://pdbe.org/4ub0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ub0 RCSB], [https://www.ebi.ac.uk/pdbsum/4ub0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ub0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 08:12, 22 March 2023

OCA — Wed, 22 Mar 2023 08:12:49 GMT

←Older revision		Revision as of 08:12, 22 March 2023
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	<StructureSection load='4ub0' size='340' side='right'caption='[[4ub0]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4ub0' size='340' side='right'caption='[[4ub0]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ub0]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UB0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4UB0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ub0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UB0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ub0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ub0 OCA], [~~http~~://pdbe.org/4ub0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ub0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ub0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ub0 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ub0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ub0 OCA], [https://pdbe.org/4ub0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ub0 RCSB], [https://www.ebi.ac.uk/pdbsum/4ub0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ub0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Acqua~~, W F.Dall~~]]	+	[[Category: Dall'Acqua WF]]
-	[[Category: Oganesyan~~, V Y]]~~	+	[[Category: Oganesyan VY]]
-	~~[[Category: Bispecific]]~~	+
-	~~[[Category: Fab]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Knobs-into-hole~~]]	+

OCA at 09:16, 21 May 2019

OCA — Tue, 21 May 2019 09:16:19 GMT

←Older revision		Revision as of 09:16, 21 May 2019
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	==New design for monovalent bispecific IgG through cysteine engineering of the CH1-CL interface==		==New design for monovalent bispecific IgG through cysteine engineering of the CH1-CL interface==
-	<StructureSection load='4ub0' size='340' side='right' caption='[[4ub0]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='4ub0' size='340' side='right'caption='[[4ub0]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ub0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UB0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ub0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UB0 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ub0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ub0 OCA], [http://pdbe.org/4ub0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ub0 RCSB], [http://www.ebi.ac.uk/pdbsum/4ub0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ub0 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ub0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ub0 OCA], [http://pdbe.org/4ub0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ub0 RCSB], [http://www.ebi.ac.uk/pdbsum/4ub0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ub0 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Acqua, W F.Dall]]		[[Category: Acqua, W F.Dall]]
	[[Category: Oganesyan, V Y]]		[[Category: Oganesyan, V Y]]

OCA at 13:27, 19 April 2017

OCA — Wed, 19 Apr 2017 13:27:24 GMT

←Older revision		Revision as of 13:27, 19 April 2017
Line 1:		Line 1:
		+
	==New design for monovalent bispecific IgG through cysteine engineering of the CH1-CL interface==		==New design for monovalent bispecific IgG through cysteine engineering of the CH1-CL interface==
	<StructureSection load='4ub0' size='340' side='right' caption='[[4ub0]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4ub0' size='340' side='right' caption='[[4ub0]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ub0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UB0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ub0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UB0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ub0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ub0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ub0 RCSB], [http://www.ebi.ac.uk/pdbsum/4ub0 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ub0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ub0 OCA], [http://pdbe.org/4ub0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ub0 RCSB], [http://www.ebi.ac.uk/pdbsum/4ub0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ub0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ub0" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:16, 15 July 2015

OCA — Wed, 15 Jul 2015 13:16:56 GMT

←Older revision		Revision as of 13:16, 15 July 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==New design for monovalent bispecific IgG through cysteine engineering of the CH1-CL interface==
		+	<StructureSection load='4ub0' size='340' side='right' caption='[[4ub0]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ub0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UB0 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ub0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ub0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ub0 RCSB], [http://www.ebi.ac.uk/pdbsum/4ub0 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Monovalent bispecific IgGs cater to a distinct set of mechanisms of action but are difficult to engineer and manufacture because of complexities associated with correct heavy and light chain pairing. We have created a novel design, "DuetMab," for efficient production of these molecules. The platform uses knobs-into-holes (KIH) technology for heterodimerization of 2 distinct heavy chains and increases the efficiency of cognate heavy and light chain pairing by replacing the native disulfide bond in one of the CH1-CL interfaces with an engineered disulfide bond. Using two pairs of antibodies, cetuximab (anti-EGFR) and trastuzumab (anti-HER2), and anti-CD40 and anti-CD70 antibodies, we demonstrate that DuetMab antibodies can be produced in a highly purified and active form, and show for the first time that monovalent bispecific IgGs can concurrently bind both antigens on the same cell. This last property compensates for the loss of avidity brought about by monovalency and improves selectivity toward the target cell.

-	~~The entry 4ub0 is ON HOLD until Paper Publication~~	+	Improving target cell specificity using a novel monovalent bispecific IgG design.,Mazor Y, Oganesyan V, Yang C, Hansen A, Wang J, Liu H, Sachsenmeier K, Carlson M, Gadre DV, Borrok MJ, Yu XQ, Dall'Acqua W, Wu H, Chowdhury PS MAbs. 2015;7(2):377-89. doi: 10.1080/19420862.2015.1007816. PMID:25621507<ref>PMID:25621507</ref>

-	~~Authors: Oganesyan~~, V.Y.~~, Dall'~~Acqua, W.F.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~New design for monovalent bispecific IgG through cysteine engineering of the CH1-CL interface~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: ~~Dall'Acqua, W.F~~]]	+	__TOC__
-	[[Category: ~~Oganesyan, V.Y~~]]	+	</StructureSection>
		+	[[Category: Acqua, W F.Dall]]
		+	[[Category: Oganesyan, V Y]]
		+	[[Category: Bispecific]]
		+	[[Category: Fab]]
		+	[[Category: Immune system]]
		+	[[Category: Knobs-into-hole]]

OCA at 18:18, 24 December 2014

OCA — Wed, 24 Dec 2014 18:18:41 GMT

←Older revision		Revision as of 18:18, 24 December 2014
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	Description: New design for monovalent bispecific IgG through cysteine engineering of the CH1-CL interface		Description: New design for monovalent bispecific IgG through cysteine engineering of the CH1-CL interface
		+	[[Category: Unreleased Structures]]
		+	[[Category: Dall'Acqua, W.F]]
		+	[[Category: Oganesyan, V.Y]]

OCA at 08:51, 24 September 2014

OCA — Wed, 24 Sep 2014 08:51:17 GMT

←Older revision		Revision as of 08:51, 24 September 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ub0 is ON HOLD	+	The entry 4ub0 is ON HOLD until Paper Publication

	Authors: Oganesyan, V.Y., Dall'Acqua, W.F.		Authors: Oganesyan, V.Y., Dall'Acqua, W.F.

	Description: New design for monovalent bispecific IgG through cysteine engineering of the CH1-CL interface		Description: New design for monovalent bispecific IgG through cysteine engineering of the CH1-CL interface

OCA: Protected "4ub0" [edit=sysop:move=sysop]

OCA — Wed, 20 Aug 2014 15:07:35 GMT

Protected "4ub0" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:07, 20 August 2014

OCA: New page: '''Unreleased structure''' The entry 4ub0 is ON HOLD Authors: Oganesyan, V.Y., Dall'Acqua, W.F. Description: New design for monovalent bispecific IgG through cysteine engineering of th...

OCA — Wed, 20 Aug 2014 15:07:33 GMT

New page: '''Unreleased structure''' The entry 4ub0 is ON HOLD Authors: Oganesyan, V.Y., Dall'Acqua, W.F. Description: New design for monovalent bispecific IgG through cysteine engineering of th...

New page

'''Unreleased structure'''

The entry 4ub0 is ON HOLD

Authors: Oganesyan, V.Y., Dall'Acqua, W.F.

Description: New design for monovalent bispecific IgG through cysteine engineering of the CH1-CL interface