4ud3 - Revision history

OCA: Redirecting to 5aam

OCA — Wed, 05 Aug 2015 19:55:42 GMT

Redirecting to 5aam

←Older revision		Revision as of 19:55, 5 August 2015
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-	~~==Structure of a redesigned cross-reactive antibody to dengue virus with increased in vivo potency==~~	+	#REDIRECT [[5aam]] This PDB entry is obsolete and replaced by 5aam
-	~~<StructureSection load='4ud3' size='340' side='right' caption='~~[[~~4ud3]], [[Resolution\|resolution]] 2.49Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[4ud3~~]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UD3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UD3 FirstGlance]. <br>	+
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ud3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ud3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ud3 RCSB], [http://www.ebi.ac.uk/pdbsum/4ud3 PDBsum]</span></td></tr>	+
-	~~</table>~~	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Dengue is the most common vector-borne viral disease, causing nearly 400 million infections yearly. Currently there are no approved therapies. Antibody epitopes that elicit weak humoral responses may not be accessible by conventional B cell panning methods. To demonstrate an alternative strategy to generating a therapeutic antibody, we employed a non-immunodominant, but functionally relevant, epitope in domain III of the E protein, and ~~engineered~~ by structure-guided methods an antibody directed to it. The resulting antibody, Ab513, exhibits high-affinity binding to, and broadly neutralizes, multiple genotypes within all four serotypes. To assess therapeutic relevance of Ab513, activity against important human clinical features of dengue was investigated. Ab513 mitigates thrombocytopenia in a humanized mouse model, resolves vascular leakage, reduces viremia to nearly undetectable levels, and protects mice in a maternal transfer model of lethal antibody-mediated enhancement. The results demonstrate that Ab513 may reduce the public health burden from dengue.	+
-		+
-	Structure-Guided Design of an Anti-dengue Antibody Directed to a Non-immunodominant Epitope.,Robinson LN, Tharakaraman K, Rowley KJ, Costa VV, Chan KR, Wong YH, Ong LC, Tan HC, Koch T, Cain D, Kirloskar R, Viswanathan K, Liew CW, Tissire H, Ramakrishnan B, Myette JR, Babcock GJ, Sasisekharan V, Alonso S, Chen J, Lescar J, Shriver Z, Ooi EE, Sasisekharan R Cell. 2015 Jul 15. pii: S0092-8674(15)00827-2. doi: 10.1016/j.cell.2015.06.057. PMID:26189681<ref>PMID:26189681</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Lescar, J]]~~	+
-	~~[[Category: Robinson, L]]~~	+
-	~~[[Category: Sasisekharan, R]]~~	+
-	~~[[Category: Wong, Y]]~~	+
-	~~[[Category: Immune system]]~~	+

OCA at 14:41, 29 July 2015

OCA — Wed, 29 Jul 2015 14:41:22 GMT

←Older revision		Revision as of 14:41, 29 July 2015
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-	'''~~Unreleased~~ structure'''	+	==Structure of a redesigned cross-reactive antibody to dengue virus with increased in vivo potency==
		+	<StructureSection load='4ud3' size='340' side='right' caption='[[4ud3]], [[Resolution\|resolution]] 2.49Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ud3]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UD3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UD3 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ud3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ud3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ud3 RCSB], [http://www.ebi.ac.uk/pdbsum/4ud3 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Dengue is the most common vector-borne viral disease, causing nearly 400 million infections yearly. Currently there are no approved therapies. Antibody epitopes that elicit weak humoral responses may not be accessible by conventional B cell panning methods. To demonstrate an alternative strategy to generating a therapeutic antibody, we employed a non-immunodominant, but functionally relevant, epitope in domain III of the E protein, and engineered by structure-guided methods an antibody directed to it. The resulting antibody, Ab513, exhibits high-affinity binding to, and broadly neutralizes, multiple genotypes within all four serotypes. To assess therapeutic relevance of Ab513, activity against important human clinical features of dengue was investigated. Ab513 mitigates thrombocytopenia in a humanized mouse model, resolves vascular leakage, reduces viremia to nearly undetectable levels, and protects mice in a maternal transfer model of lethal antibody-mediated enhancement. The results demonstrate that Ab513 may reduce the public health burden from dengue.

-	~~The entry 4ud3 is ON HOLD until Paper Publication~~	+	Structure-Guided Design of an Anti-dengue Antibody Directed to a Non-immunodominant Epitope.,Robinson LN, Tharakaraman K, Rowley KJ, Costa VV, Chan KR, Wong YH, Ong LC, Tan HC, Koch T, Cain D, Kirloskar R, Viswanathan K, Liew CW, Tissire H, Ramakrishnan B, Myette JR, Babcock GJ, Sasisekharan V, Alonso S, Chen J, Lescar J, Shriver Z, Ooi EE, Sasisekharan R Cell. 2015 Jul 15. pii: S0092-8674(15)00827-2. doi: 10.1016/j.cell.2015.06.057. PMID:26189681<ref>PMID:26189681</ref>

-	~~Authors: Wong~~, Y.~~, Robinson, L~~.~~, Lescar, J., Sasisekharan, R~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Structure of a redesigned cross-reactive antibody to dengue virus with increased in vivo potency~~	+	== References ==
-	~~[[Category: Unreleased Structures]]~~	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Lescar, J]]		[[Category: Lescar, J]]
	[[Category: Robinson, L]]		[[Category: Robinson, L]]
		+	[[Category: Sasisekharan, R]]
	[[Category: Wong, Y]]		[[Category: Wong, Y]]
-	[[Category: ~~Sasisekharan, R~~]]	+	[[Category: Immune system]]

OCA at 18:24, 24 December 2014

OCA — Wed, 24 Dec 2014 18:24:10 GMT

←Older revision		Revision as of 18:24, 24 December 2014
Line 6:		Line 6:

	Description: Structure of a redesigned cross-reactive antibody to dengue virus with increased in vivo potency		Description: Structure of a redesigned cross-reactive antibody to dengue virus with increased in vivo potency
		+	[[Category: Unreleased Structures]]
		+	[[Category: Lescar, J]]
		+	[[Category: Robinson, L]]
		+	[[Category: Wong, Y]]
		+	[[Category: Sasisekharan, R]]

OCA at 09:39, 24 December 2014

OCA — Wed, 24 Dec 2014 09:39:42 GMT

←Older revision		Revision as of 09:39, 24 December 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ud3 is ON HOLD	+	The entry 4ud3 is ON HOLD until Paper Publication

	Authors: Wong, Y., Robinson, L., Lescar, J., Sasisekharan, R.		Authors: Wong, Y., Robinson, L., Lescar, J., Sasisekharan, R.

	Description: Structure of a redesigned cross-reactive antibody to dengue virus with increased in vivo potency		Description: Structure of a redesigned cross-reactive antibody to dengue virus with increased in vivo potency

OCA: Protected "4ud3" [edit=sysop:move=sysop]

OCA — Wed, 17 Dec 2014 11:20:27 GMT

Protected "4ud3" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:20, 17 December 2014

OCA: New page: '''Unreleased structure''' The entry 4ud3 is ON HOLD Authors: Wong, Y., Robinson, L., Lescar, J., Sasisekharan, R. Description: Structure of a redesigned cross-reactive antibody to den...

OCA — Wed, 17 Dec 2014 11:20:25 GMT

New page: '''Unreleased structure''' The entry 4ud3 is ON HOLD Authors: Wong, Y., Robinson, L., Lescar, J., Sasisekharan, R. Description: Structure of a redesigned cross-reactive antibody to den...

New page

'''Unreleased structure'''

The entry 4ud3 is ON HOLD

Authors: Wong, Y., Robinson, L., Lescar, J., Sasisekharan, R.

Description: Structure of a redesigned cross-reactive antibody to dengue virus with increased in vivo potency