4ufr - Revision history

OCA at 08:22, 22 March 2023

OCA — Wed, 22 Mar 2023 08:22:13 GMT

←Older revision		Revision as of 08:22, 22 March 2023
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	<StructureSection load='4ufr' size='340' side='right'caption='[[4ufr]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4ufr' size='340' side='right'caption='[[4ufr]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ufr]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UFR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4UFR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ufr]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UFR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UFR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ufs\|4ufs]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ufr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ufr OCA], [https://pdbe.org/4ufr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ufr RCSB], [https://www.ebi.ac.uk/pdbsum/4ufr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ufr ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ufr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ufr OCA], [~~http~~://pdbe.org/4ufr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ufr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ufr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ufr ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LGR5_HUMAN LGR5_HUMAN]] Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicle. Target gene of Wnt signaling. [[http://www.uniprot.org/uniprot/RSPO2_MOUSE RSPO2_MOUSE]] Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors.<ref>PMID:21693646</ref>	+	[https://www.uniprot.org/uniprot/LGR5_HUMAN LGR5_HUMAN] Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicle. Target gene of Wnt signaling.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jones~~, E Y~~]]	+	[[Category: Jones EY]]
-	[[Category: Zebisch, M]]	+	[[Category: Zebisch M]]
-	~~[[Category: Lgr]]~~	+
-	~~[[Category: Rspo]]~~	+
-	~~[[Category: Signaling protein]]~~	+
-	~~[[Category: Wnt~~]]	+

OCA at 10:54, 26 February 2020

OCA — Wed, 26 Feb 2020 10:54:20 GMT

←Older revision		Revision as of 10:54, 26 February 2020
Line 1:		Line 1:

	==Structure of the ectodomain of LGR5 in complex with R-spondin-2 (Fu1Fu2)==		==Structure of the ectodomain of LGR5 in complex with R-spondin-2 (Fu1Fu2)==
-	<StructureSection load='4ufr' size='340' side='right' caption='[[4ufr]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='4ufr' size='340' side='right'caption='[[4ufr]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ufr]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UFR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UFR FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ufr]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UFR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UFR FirstGlance]. <br>
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Jones, E Y]]		[[Category: Jones, E Y]]
	[[Category: Zebisch, M]]		[[Category: Zebisch, M]]

OCA at 16:14, 16 November 2017

OCA — Thu, 16 Nov 2017 16:14:37 GMT

←Older revision		Revision as of 16:14, 16 November 2017
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		+
	==Structure of the ectodomain of LGR5 in complex with R-spondin-2 (Fu1Fu2)==		==Structure of the ectodomain of LGR5 in complex with R-spondin-2 (Fu1Fu2)==
	<StructureSection load='4ufr' size='340' side='right' caption='[[4ufr]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4ufr' size='340' side='right' caption='[[4ufr]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ufr]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UFR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UFR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ufr]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UFR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UFR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ufs\|4ufs]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ufs\|4ufs]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ufr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ufr OCA], [http://pdbe.org/4ufr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ufr RCSB], [http://www.ebi.ac.uk/pdbsum/4ufr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ufr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ufr OCA], [http://pdbe.org/4ufr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ufr RCSB], [http://www.ebi.ac.uk/pdbsum/4ufr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ufr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Jones, E Y]]		[[Category: Jones, E Y]]
	[[Category: Zebisch, M]]		[[Category: Zebisch, M]]

OCA at 06:49, 30 September 2015

OCA — Wed, 30 Sep 2015 06:49:37 GMT

←Older revision		Revision as of 06:49, 30 September 2015
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ufs\|4ufs]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ufs\|4ufs]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ufr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ufr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ufr RCSB], [http://www.ebi.ac.uk/pdbsum/4ufr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ufr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ufr OCA], [http://pdbe.org/4ufr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ufr RCSB], [http://www.ebi.ac.uk/pdbsum/4ufr PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ufr" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:35, 15 July 2015

OCA — Wed, 15 Jul 2015 07:35:33 GMT

←Older revision		Revision as of 07:35, 15 July 2015
Line 1:		Line 1:
-	==~~WNT SIGNALLING COMPLEX~~==	+	==Structure of the ectodomain of LGR5 in complex with R-spondin-2 (Fu1Fu2)==
	<StructureSection load='4ufr' size='340' side='right' caption='[[4ufr]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4ufr' size='340' side='right' caption='[[4ufr]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==

OCA at 14:48, 8 July 2015

OCA — Wed, 08 Jul 2015 14:48:03 GMT

←Older revision		Revision as of 14:48, 8 July 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==WNT SIGNALLING COMPLEX==
		+	<StructureSection load='4ufr' size='340' side='right' caption='[[4ufr]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ufr]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UFR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UFR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ufs\|4ufs]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ufr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ufr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ufr RCSB], [http://www.ebi.ac.uk/pdbsum/4ufr PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LGR5_HUMAN LGR5_HUMAN]] Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicle. Target gene of Wnt signaling. [[http://www.uniprot.org/uniprot/RSPO2_MOUSE RSPO2_MOUSE]] Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors.<ref>PMID:21693646</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The four secreted R-spondin (Rspo1-4) proteins of vertebrates function as stem cell growth factors and potentiate canonical Wnt signalling. Rspo proteins act by cross-linking members of two cell surface receptor families, complexing the stem cell markers LGR4-6 with the Frizzled-specific E3 ubiquitin ligases ZNRF3/RNF43. The consequent internalisation of the ternary LGR-Rspo-E3 complex removes the E3 ligase activity, which otherwise targets the Wnt receptor Frizzled for degradation, and thus enhances Wnt signalling. Multiple combinations of LGR4-6, Rspo1-4 and ZNRF3/RNF43 are possible, implying the existence of generic interaction determinants, but also of specific differences in complex architecture and activity. We present here a high resolution crystal structure of an ectodomain variant of human LGR5 (hLGR5ecto) complexed with a signalling competent fragment of mouse Rspo2 (mRspo2Fu1-Fu2). The structure shows that the particularly potent Rspo2 ligand engages LGR5 in a fashion almost identical to that reported for hRSPO1. Comparison of our hLGR5ecto structure with previously published structures highlights a surprising plasticity of the LGR ectodomains, characterised by a nearly 9 degrees or larger rotation of the N-terminal half of the horseshoe-like fold relative to the C-terminal half. We also report a low resolution hLGR5-mRspo2Fu1-Fu2-mZNRF3ecto ternary complex structure. This crystal structure confirms our previously suggested hypothesis, showing that Rspo proteins cross-link LGRs and ZNRF3 into a 2:2:2 complex, whereas a 1:1:1 complex is formed with RNF43.

-	~~The entry 4ufr is ON HOLD~~	+	Crystal structure of R-spondin 2 in complex with the ectodomains of its receptors LGR5 and ZNRF3.,Zebisch M, Yvonne Jones E J Struct Biol. 2015 Jun 26. pii: S1047-8477(15)00137-9. doi:, 10.1016/j.jsb.2015.05.008. PMID:26123262<ref>PMID:26123262</ref>

-	~~Authors: Zebisch~~, M.~~, Jones, E~~.Y.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: WNT SIGNALLING COMPLEX~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Jones, E Y]]
	[[Category: Zebisch, M]]		[[Category: Zebisch, M]]
-	[[Category: ~~Jones, E.Y~~]]	+	[[Category: Lgr]]
		+	[[Category: Rspo]]
		+	[[Category: Signaling protein]]
		+	[[Category: Wnt]]

OCA: Protected "4ufr" [edit=sysop:move=sysop]

OCA — Thu, 26 Mar 2015 10:38:43 GMT

Protected "4ufr" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:38, 26 March 2015

OCA: New page: '''Unreleased structure''' The entry 4ufr is ON HOLD Authors: Zebisch, M., Jones, E.Y. Description: WNT SIGNALLING COMPLEX Category: Unreleased Structures Category: Zebisch, M ...

OCA — Thu, 26 Mar 2015 10:38:41 GMT

New page: '''Unreleased structure''' The entry 4ufr is ON HOLD Authors: Zebisch, M., Jones, E.Y. Description: WNT SIGNALLING COMPLEX Category: Unreleased Structures Category: Zebisch, M ...

New page

'''Unreleased structure'''

The entry 4ufr is ON HOLD

Authors: Zebisch, M., Jones, E.Y.

Description: WNT SIGNALLING COMPLEX
[[Category: Unreleased Structures]]
[[Category: Zebisch, M]]
[[Category: Jones, E.Y]]