4uhk - Revision history

OCA at 08:24, 22 March 2023

OCA — Wed, 22 Mar 2023 08:24:22 GMT

←Older revision		Revision as of 08:24, 22 March 2023
Line 3:		Line 3:
	<StructureSection load='4uhk' size='340' side='right'caption='[[4uhk]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='4uhk' size='340' side='right'caption='[[4uhk]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4uhk]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4UHK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4uhk]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UHK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PHD:ASPARTYL+PHOSPHATE'>PHD</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=PHD:ASPARTYL+PHOSPHATE'>PHD</scene>~~</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhk OCA], [https://pdbe.org/4uhk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uhk RCSB], [https://www.ebi.ac.uk/pdbsum/4uhk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uhk ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4uhj\|4uhj]], [[4uhs\|4uhs]], [[4uht\|4uht]]~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4uhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhk OCA], [~~http~~://pdbe.org/4uhk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4uhk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4uhk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4uhk ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CPXR_ECOLI CPXR_ECOLI]] Response regulator member of the two-component regulatory system CpxA/CpxR which responds to envelope stress response by activating expression of downstream genes including cpxP, degP, dsbA and ppiA (PubMed:7883164, PubMed:9401031). Binds and activates transcription from the degP promoter (PubMed:7883164); binding is enhanced by phosphorylation (PubMed:9401031). This system combats a variety of extracytoplasmic protein-mediated toxicities by increasing the transcription of the periplasmic protease, DegP in concert with sigma factor E (PubMed:7883164), as well as that of CpxP protein. Other downstream effectors may include SrkA (PubMed:23416055).<ref>PMID:23416055</ref> <ref>PMID:7883164</ref> <ref>PMID:9401031</ref>	+	[https://www.uniprot.org/uniprot/CPXR_ECOLI CPXR_ECOLI] Response regulator member of the two-component regulatory system CpxA/CpxR which responds to envelope stress response by activating expression of downstream genes including cpxP, degP, dsbA and ppiA (PubMed:7883164, PubMed:9401031). Binds and activates transcription from the degP promoter (PubMed:7883164); binding is enhanced by phosphorylation (PubMed:9401031). This system combats a variety of extracytoplasmic protein-mediated toxicities by increasing the transcription of the periplasmic protease, DegP in concert with sigma factor E (PubMed:7883164), as well as that of CpxP protein. Other downstream effectors may include SrkA (PubMed:23416055).<ref>PMID:23416055</ref> <ref>PMID:7883164</ref> <ref>PMID:9401031</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Alzari~~, P M.A~~]]	+	[[Category: Alzari PMA]]
-	[[Category: Mechaly~~, A E]]~~	+	[[Category: Mechaly AE]]
-	~~[[Category: Transcription~~]]	+

OCA at 10:51, 26 February 2020

OCA — Wed, 26 Feb 2020 10:51:04 GMT

←Older revision		Revision as of 10:51, 26 February 2020
Line 1:		Line 1:

	==Crystal structure of the receiver domain of CpxR from E. coli (phosphorylated)==		==Crystal structure of the receiver domain of CpxR from E. coli (phosphorylated)==
-	<StructureSection load='4uhk' size='340' side='right' caption='[[4uhk]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='4uhk' size='340' side='right'caption='[[4uhk]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4uhk]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UHK FirstGlance]. <br>		<table><tr><td colspan='2'>[[4uhk]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UHK FirstGlance]. <br>
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Alzari, P M.A]]		[[Category: Alzari, P M.A]]
	[[Category: Mechaly, A E]]		[[Category: Mechaly, A E]]
	[[Category: Transcription]]		[[Category: Transcription]]

OCA at 10:30, 3 August 2017

OCA — Thu, 03 Aug 2017 10:30:32 GMT

←Older revision		Revision as of 10:30, 3 August 2017
Line 7:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PHD:ASPARTYL+PHOSPHATE'>PHD</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PHD:ASPARTYL+PHOSPHATE'>PHD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4uhj\|4uhj]], [[4uhs\|4uhs]], [[4uht\|4uht]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4uhj\|4uhj]], [[4uhs\|4uhs]], [[4uht\|4uht]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhk OCA], [http://pdbe.org/4uhk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uhk RCSB], [http://www.ebi.ac.uk/pdbsum/4uhk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhk OCA], [http://pdbe.org/4uhk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uhk RCSB], [http://www.ebi.ac.uk/pdbsum/4uhk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4uhk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/CPXR_ECOLI CPXR_ECOLI]] Response regulator member of the two-component regulatory system CpxA/CpxR which responds to envelope stress response by activating expression of downstream genes including cpxP, degP, dsbA and ppiA (PubMed:7883164, PubMed:9401031). Binds and activates transcription from the degP promoter (PubMed:7883164); binding is enhanced by phosphorylation (PubMed:9401031). This system combats a variety of extracytoplasmic protein-mediated toxicities by increasing the transcription of the periplasmic protease, DegP in concert with sigma factor E (PubMed:7883164), as well as that of CpxP protein. Other downstream effectors may include SrkA (PubMed:23416055).<ref>PMID:23416055</ref> <ref>PMID:7883164</ref> <ref>PMID:9401031</ref>		[[http://www.uniprot.org/uniprot/CPXR_ECOLI CPXR_ECOLI]] Response regulator member of the two-component regulatory system CpxA/CpxR which responds to envelope stress response by activating expression of downstream genes including cpxP, degP, dsbA and ppiA (PubMed:7883164, PubMed:9401031). Binds and activates transcription from the degP promoter (PubMed:7883164); binding is enhanced by phosphorylation (PubMed:9401031). This system combats a variety of extracytoplasmic protein-mediated toxicities by increasing the transcription of the periplasmic protease, DegP in concert with sigma factor E (PubMed:7883164), as well as that of CpxP protein. Other downstream effectors may include SrkA (PubMed:23416055).<ref>PMID:23416055</ref> <ref>PMID:7883164</ref> <ref>PMID:9401031</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial two-component systems consist of a sensor histidine kinase (HK) and a response regulator (RR). HKs are homodimers that catalyze the autophosphorylation of a histidine residue and the subsequent phosphoryl transfer to its RR partner, triggering an adaptive response. How the HK autokinase and phosphotransferase activities are coordinated remains unclear. Here, we report X-ray structures of the prototypical HK CpxA trapped as a hemi-phosphorylated dimer, and of the receiver domain from the RR partner, CpxR. Our results reveal that the two catalytic reactions can occur simultaneously, one in each protomer of the asymmetric CpxA dimer. Furthermore, the increase of autokinase activity in the presence of phosphotransfer-impaired CpxR put forward the idea of an allosteric switching mechanism, according to which CpxR binding to one CpxA protomer triggers autophosphorylation in the second protomer. The ensuing dynamical model provides a mechanistic explanation of how HKs can efficiently orchestrate two catalytic reactions involving large-scale protein motions.
		+
		+	Structural Coupling between Autokinase and Phosphotransferase Reactions in a Bacterial Histidine Kinase.,Mechaly AE, Soto Diaz S, Sassoon N, Buschiazzo A, Betton JM, Alzari PM Structure. 2017 Jun 6;25(6):939-944.e3. doi: 10.1016/j.str.2017.04.011. Epub 2017, May 25. PMID:28552574<ref>PMID:28552574</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4uhk" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:16, 12 May 2016

OCA — Thu, 12 May 2016 11:16:44 GMT

←Older revision		Revision as of 11:16, 12 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 4uhk is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of the receiver domain of CpxR from E. coli (phosphorylated)==
-		+	<StructureSection load='4uhk' size='340' side='right' caption='[[4uhk]], [[Resolution\|resolution]] 2.60Å' scene=''>
-	~~Authors~~: ~~Mechaly~~, A.E., ~~Alzari~~, P.M.A.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4uhk]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UHK FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure~~ of the ~~receiver domain of~~ CpxR from E. ~~coli~~ ( ~~phosphorylated~~)	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PHD:ASPARTYL+PHOSPHATE'>PHD</scene></td></tr>
-	[[Category: Alzari, P.M.A]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4uhj\|4uhj]], [[4uhs\|4uhs]], [[4uht\|4uht]]</td></tr>
-	[[Category: Mechaly, A.E]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhk OCA], [http://pdbe.org/4uhk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uhk RCSB], [http://www.ebi.ac.uk/pdbsum/4uhk PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CPXR_ECOLI CPXR_ECOLI]] Response regulator member of the two-component regulatory system CpxA/CpxR which responds to envelope stress response by activating expression of downstream genes including cpxP, degP, dsbA and ppiA (PubMed:7883164, PubMed:9401031). Binds and activates transcription from the degP promoter (PubMed:7883164); binding is enhanced by phosphorylation (PubMed:9401031). This system combats a variety of extracytoplasmic protein-mediated toxicities by increasing the transcription of the periplasmic protease, DegP in concert with sigma factor E (PubMed:7883164), as well as that of CpxP protein. Other downstream effectors may include SrkA (PubMed:23416055).<ref>PMID:23416055</ref> <ref>PMID:7883164</ref> <ref>PMID:9401031</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Alzari, P M.A]]
		+	[[Category: Mechaly, A E]]
		+	[[Category: Transcription]]

OCA at 07:37, 2 March 2016

OCA — Wed, 02 Mar 2016 07:37:46 GMT

←Older revision		Revision as of 07:37, 2 March 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4uhk is ON HOLD	+	The entry 4uhk is ON HOLD until Paper Publication

	Authors: Mechaly, A.E., Alzari, P.M.A.		Authors: Mechaly, A.E., Alzari, P.M.A.

OCA at 22:57, 30 November 2015

OCA — Mon, 30 Nov 2015 22:57:50 GMT

←Older revision		Revision as of 22:57, 30 November 2015
Line 7:		Line 7:
	Description: Crystal structure of the receiver domain of CpxR from E. coli ( phosphorylated)		Description: Crystal structure of the receiver domain of CpxR from E. coli ( phosphorylated)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Mechaly, A.E]]
	[[Category: Alzari, P.M.A]]		[[Category: Alzari, P.M.A]]
		+	[[Category: Mechaly, A.E]]

OCA: Protected "4uhk" [edit=sysop:move=sysop]

OCA — Wed, 01 Apr 2015 09:44:14 GMT

Protected "4uhk" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:44, 1 April 2015

OCA: New page: '''Unreleased structure''' The entry 4uhk is ON HOLD Authors: Mechaly, A.E., Alzari, P.M.A. Description: Crystal structure of the receiver domain of CpxR from E. coli ( phosphorylated)...

OCA — Wed, 01 Apr 2015 09:44:13 GMT

New page: '''Unreleased structure''' The entry 4uhk is ON HOLD Authors: Mechaly, A.E., Alzari, P.M.A. Description: Crystal structure of the receiver domain of CpxR from E. coli ( phosphorylated)...

New page

'''Unreleased structure'''

The entry 4uhk is ON HOLD

Authors: Mechaly, A.E., Alzari, P.M.A.

Description: Crystal structure of the receiver domain of CpxR from E. coli ( phosphorylated)
[[Category: Unreleased Structures]]
[[Category: Mechaly, A.E]]
[[Category: Alzari, P.M.A]]