4uhp - Revision history

OCA at 10:29, 10 January 2024

OCA — Wed, 10 Jan 2024 10:29:36 GMT

←Older revision		Revision as of 10:29, 10 January 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4uhp]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UHP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4uhp]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UHP FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uhp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhp OCA], [https://pdbe.org/4uhp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uhp RCSB], [https://www.ebi.ac.uk/pdbsum/4uhp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uhp ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uhp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhp OCA], [https://pdbe.org/4uhp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uhp RCSB], [https://www.ebi.ac.uk/pdbsum/4uhp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uhp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 08:24, 22 March 2023

OCA — Wed, 22 Mar 2023 08:24:30 GMT

←Older revision		Revision as of 08:24, 22 March 2023
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	<StructureSection load='4uhp' size='340' side='right'caption='[[4uhp]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4uhp' size='340' side='right'caption='[[4uhp]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4uhp]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4UHP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4uhp]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UHP FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4uhq\|4uhq]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uhp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhp OCA], [https://pdbe.org/4uhp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uhp RCSB], [https://www.ebi.ac.uk/pdbsum/4uhp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uhp ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4uhp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhp OCA], [~~http~~://pdbe.org/4uhp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4uhp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4uhp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4uhp ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q51502_PSEAI Q51502_PSEAI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pseae~~]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: Chen, S]]	+	[[Category: Chen S]]
-	[[Category: Joshi, A]]	+	[[Category: Joshi A]]
-	[[Category: Kaminska, R]]	+	[[Category: Kaminska R]]
-	[[Category: Kleanthous, C]]	+	[[Category: Kleanthous C]]
-	[[Category: Wojdyla~~, J A]]~~	+	[[Category: Wojdyla JA]]
-	~~[[Category: Bacteriocin]]~~	+
-	~~[[Category: Dnase]]~~	+
-	~~[[Category: Dnase-im]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Pyocin~~]]	+

OCA at 08:43, 21 May 2019

OCA — Tue, 21 May 2019 08:43:49 GMT

←Older revision		Revision as of 08:43, 21 May 2019
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		+
	==Crystal structure of the pyocin AP41 DNase-Immunity complex==		==Crystal structure of the pyocin AP41 DNase-Immunity complex==
-	<StructureSection load='4uhp' size='340' side='right' caption='[[4uhp]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4uhp' size='340' side='right'caption='[[4uhp]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4uhp]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UHP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4uhp]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UHP FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4uhq\|4uhq]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4uhq\|4uhq]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uhp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4uhp RCSB], [http://www.ebi.ac.uk/pdbsum/4uhp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uhp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhp OCA], [http://pdbe.org/4uhp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uhp RCSB], [http://www.ebi.ac.uk/pdbsum/4uhp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4uhp ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4uhp" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pseae]]
	[[Category: Chen, S]]		[[Category: Chen, S]]
	[[Category: Joshi, A]]		[[Category: Joshi, A]]

OCA at 20:06, 5 August 2015

OCA — Wed, 05 Aug 2015 20:06:13 GMT

←Older revision		Revision as of 20:06, 5 August 2015
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-	'''~~Unreleased~~ structure'''	+	==Crystal structure of the pyocin AP41 DNase-Immunity complex==
		+	<StructureSection load='4uhp' size='340' side='right' caption='[[4uhp]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4uhp]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UHP FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4uhq\|4uhq]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uhp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4uhp RCSB], [http://www.ebi.ac.uk/pdbsum/4uhp PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	How ultra-high affinity protein-protein interactions retain high specificity is still poorly understood. The interaction between colicin DNase domains and their inhibitory immunity (Im) proteins is an ultra-high affinity interaction that is essential for the neutralisation of endogenous DNase catalytic activity and for protection against exogenous DNase bacteriocins. The colicin DNase-Im interaction is a model system for the study of high affinity protein-protein interactions. However, despite the fact that closely related colicin-like bacteriocins are widely produced by Gram-negative bacteria this interaction has only been studied using the colicins from Escherichia coli. In this work, we present the first crystal structures of two pyocin DNase-Im complexes from Pseudomonas aeruginosa, pyocin S2 DNase-ImS2 and pyocin AP41 DNase-ImAP41. These structures represent divergent DNase-Im subfamilies and are important in extending our understanding of protein-protein interactions for this important class of high affinity protein complex. A key finding of this work is that mutations within the immunity protein binding energy hotspot, helix III, are tolerated by complementary substitutions at the DNase-immunity protein binding interface. Im helix III is strictly conserved in colicins where an Asp forms polar interactions with the DNase backbone. ImAP41 contains an Asp-to-Gly substitution in helix III and our structures show the role of a co-evolved substitution where Pro in DNase loop 4 occupies the volume vacated and removes the unfulfilled hydrogen bond. We observe the co-evolved mutations in other DNase-immunity pairs that appear to underpin the split of this family into two distinct groups.

-	~~The entry 4uhp is ON HOLD until Paper Publication~~	+	Structures of the ultra-high affinity protein-protein complexes of pyocins S2 and AP41 and their cognate immunity proteins from Pseudomonas aeruginosa.,Joshi A, Grinter R, Josts I, Chen S, Wojdyla JA, Lowe ED, Kaminska R, Sharp C, McCaughey L, Roszak AW, Cogdell RJ, Byron O, Walker D, Kleanthous C J Mol Biol. 2015 Jul 24. pii: S0022-2836(15)00395-2. doi:, 10.1016/j.jmb.2015.07.014. PMID:26215615<ref>PMID:26215615</ref>

-	~~Authors: Joshi~~, A.~~, Chen,~~ S.~~, Wojdyla, J.A., Kaminska, R., Kleanthous, C~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Crystal structure of the pyocin AP41 DNase-Immunity complex~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chen, S]]
	[[Category: Joshi, A]]		[[Category: Joshi, A]]
-	[[Category: ~~Wojdyla~~, ~~J.A~~]]	+	[[Category: Kaminska, R]]
	[[Category: Kleanthous, C]]		[[Category: Kleanthous, C]]
-	[[Category: ~~Kaminska~~, R]]	+	[[Category: Wojdyla, J A]]
-	[[Category: ~~Chen, S~~]]	+	[[Category: Bacteriocin]]
		+	[[Category: Dnase]]
		+	[[Category: Dnase-im]]
		+	[[Category: Hydrolase]]
		+	[[Category: Pyocin]]

OCA at 07:45, 8 April 2015

OCA — Wed, 08 Apr 2015 07:45:01 GMT

←Older revision		Revision as of 07:45, 8 April 2015
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4uhp is ON HOLD	+	The entry 4uhp is ON HOLD until Paper Publication

	Authors: Joshi, A., Chen, S., Wojdyla, J.A., Kaminska, R., Kleanthous, C.		Authors: Joshi, A., Chen, S., Wojdyla, J.A., Kaminska, R., Kleanthous, C.

OCA: Protected "4uhp" [edit=sysop:move=sysop]

OCA — Wed, 01 Apr 2015 09:44:27 GMT

Protected "4uhp" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:44, 1 April 2015

OCA: New page: '''Unreleased structure''' The entry 4uhp is ON HOLD Authors: Joshi, A., Chen, S., Wojdyla, J.A., Kaminska, R., Kleanthous, C. Description: Crystal structure of the pyocin AP41 DNase-I...

OCA — Wed, 01 Apr 2015 09:44:26 GMT

New page: '''Unreleased structure''' The entry 4uhp is ON HOLD Authors: Joshi, A., Chen, S., Wojdyla, J.A., Kaminska, R., Kleanthous, C. Description: Crystal structure of the pyocin AP41 DNase-I...

New page

'''Unreleased structure'''

The entry 4uhp is ON HOLD

Authors: Joshi, A., Chen, S., Wojdyla, J.A., Kaminska, R., Kleanthous, C.

Description: Crystal structure of the pyocin AP41 DNase-Immunity complex
[[Category: Unreleased Structures]]
[[Category: Joshi, A]]
[[Category: Wojdyla, J.A]]
[[Category: Kleanthous, C]]
[[Category: Kaminska, R]]
[[Category: Chen, S]]