4uq5 - Revision history

OCA: Redirecting to 4ujd

OCA — Wed, 10 Dec 2014 15:57:51 GMT

Redirecting to 4ujd

←Older revision		Revision as of 15:57, 10 December 2014
Line 1:		Line 1:
-	~~==mammalian 80S HCV-IRES initiation complex with eIF5B PRE-like state==~~	+	#REDIRECT [[4ujd]] This PDB entry is obsolete and replaced by 4ujd
-	~~<StructureSection load='4uq5' size='340' side='right' caption='~~[[~~4uq5]], [[Resolution\|resolution]] 8.90Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[4uq5~~]] is a 34 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UQ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UQ5 FirstGlance]. <br>	+
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4upw\|4upw]], [[4upx\|4upx]], [[4upy\|4upy]], [[4upz\|4upz]], [[4uq0\|4uq0]], [[4uq1\|4uq1]], [[4uq4\|4uq4]]</td></tr>	+
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uq5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4uq5 RCSB], [http://www.ebi.ac.uk/pdbsum/4uq5 PDBsum]</span></td></tr>	+
-	~~<table>~~	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	~~The universally conserved eukaryotic initiation factor (eIF) 5B, a translational GTPase, is essential for canonical translation initiation. It is also required for initiation facilitated~~ by the internal ribosomal entry site (IRES) of hepatitis C virus (HCV) RNA. eIF5B promotes joining of 60S ribosomal subunits to 40S ribosomal subunits bound by initiator tRNA (Met-tRNAiMet). However, the exact molecular mechanism by which eIF5B acts has not been established. Here we present cryo-EM reconstructions of the mammalian 80S-HCV-IRES-Met-tRNAiMet-eIF5B-GMPPNP complex. We obtained two substates distinguished by the rotational state of the ribosomal subunits and the configuration of initiator tRNA in the peptidyl (P) site. Accordingly, a combination of conformational changes in the 80S ribosome and in initiator tRNA facilitates binding of the Met-tRNAiMet to the 60S P site and redefines the role of eIF5B as a tRNA-reorientation factor.	+
-		+
-	Structure of the mammalian 80S initiation complex with initiation factor 5B on HCV-IRES RNA.,Yamamoto H, Unbehaun A, Loerke J, Behrmann E, Collier M, Burger J, Mielke T, Spahn CM Nat Struct Mol Biol. 2014 Aug;21(8):721-727. doi: 10.1038/nsmb.2859. Epub 2014, Jul 27. PMID:25064512<ref>PMID:25064512</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Oryctolagus cuniculus]]~~	+
-	~~[[Category: Behrmann, E.]]~~	+
-	~~[[Category: Burger, J.]]~~	+
-	~~[[Category: Loerke, J.]]~~	+
-	~~[[Category: Marianne, C.]]~~	+
-	~~[[Category: Mielke, T.]]~~	+
-	~~[[Category: Spahn, C M.T.]]~~	+
-	~~[[Category: Unbehaun, A.]]~~	+
-	~~[[Category: Yamamoto, H.]]~~	+
-	~~[[Category: Eukaryotic initiation factor 5b]]~~	+
-	~~[[Category: Hepatitis c virus internal ribosome entry site]]~~	+
-	~~[[Category: Ribosome]]~~	+
-	~~[[Category: Translation initiation]]~~	+

OCA at 02:31, 7 August 2014

OCA — Thu, 07 Aug 2014 02:31:16 GMT

←Older revision		Revision as of 02:31, 7 August 2014
Line 6:		Line 6:
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uq5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4uq5 RCSB], [http://www.ebi.ac.uk/pdbsum/4uq5 PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uq5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4uq5 RCSB], [http://www.ebi.ac.uk/pdbsum/4uq5 PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The universally conserved eukaryotic initiation factor (eIF) 5B, a translational GTPase, is essential for canonical translation initiation. It is also required for initiation facilitated by the internal ribosomal entry site (IRES) of hepatitis C virus (HCV) RNA. eIF5B promotes joining of 60S ribosomal subunits to 40S ribosomal subunits bound by initiator tRNA (Met-tRNAiMet). However, the exact molecular mechanism by which eIF5B acts has not been established. Here we present cryo-EM reconstructions of the mammalian 80S-HCV-IRES-Met-tRNAiMet-eIF5B-GMPPNP complex. We obtained two substates distinguished by the rotational state of the ribosomal subunits and the configuration of initiator tRNA in the peptidyl (P) site. Accordingly, a combination of conformational changes in the 80S ribosome and in initiator tRNA facilitates binding of the Met-tRNAiMet to the 60S P site and redefines the role of eIF5B as a tRNA-reorientation factor.
		+
		+	Structure of the mammalian 80S initiation complex with initiation factor 5B on HCV-IRES RNA.,Yamamoto H, Unbehaun A, Loerke J, Behrmann E, Collier M, Burger J, Mielke T, Spahn CM Nat Struct Mol Biol. 2014 Aug;21(8):721-727. doi: 10.1038/nsmb.2859. Epub 2014, Jul 27. PMID:25064512<ref>PMID:25064512</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:56, 30 July 2014

OCA — Wed, 30 Jul 2014 07:56:26 GMT

←Older revision		Revision as of 07:56, 30 July 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==mammalian 80S HCV-IRES initiation complex with eIF5B PRE-like state==
-		+	<StructureSection load='4uq5' size='340' side='right' caption='[[4uq5]], [[Resolution\|resolution]] 8.90Å' scene=''>
-	~~The entry~~ 4uq5 is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4uq5]] is a 34 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UQ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UQ5 FirstGlance]. <br>
-	~~Authors~~: ~~Yamamoto~~, H., ~~Unbehaun~~, A., ~~Loerke~~, J., Behrmann, E., ~~Marianne, C~~.~~, Burger~~, J., Mielke, T., Spahn, C.M.T.	+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4upw\|4upw]], [[4upx\|4upx]], [[4upy\|4upy]], [[4upz\|4upz]], [[4uq0\|4uq0]], [[4uq1\|4uq1]], [[4uq4\|4uq4]]</td></tr>
-		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uq5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4uq5 RCSB], [http://www.ebi.ac.uk/pdbsum/4uq5 PDBsum]</span></td></tr>
-	~~Description~~: ~~mammalian 80S HCV-IRES~~ initiation ~~complex with eIF5B PRE-like state~~	+	<table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Oryctolagus cuniculus]]
		+	[[Category: Behrmann, E.]]
		+	[[Category: Burger, J.]]
		+	[[Category: Loerke, J.]]
		+	[[Category: Marianne, C.]]
		+	[[Category: Mielke, T.]]
		+	[[Category: Spahn, C M.T.]]
		+	[[Category: Unbehaun, A.]]
		+	[[Category: Yamamoto, H.]]
		+	[[Category: Eukaryotic initiation factor 5b]]
		+	[[Category: Hepatitis c virus internal ribosome entry site]]
		+	[[Category: Ribosome]]
		+	[[Category: Translation initiation]]

OCA at 09:59, 16 July 2014

OCA — Wed, 16 Jul 2014 09:59:52 GMT

←Older revision		Revision as of 09:59, 16 July 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4uq5 is ON HOLD	+	The entry 4uq5 is ON HOLD until Paper Publication

	Authors: Yamamoto, H., Unbehaun, A., Loerke, J., Behrmann, E., Marianne, C., Burger, J., Mielke, T., Spahn, C.M.T.		Authors: Yamamoto, H., Unbehaun, A., Loerke, J., Behrmann, E., Marianne, C., Burger, J., Mielke, T., Spahn, C.M.T.

-	Description: mammalian 80S initiation complex with eIF5B PRE-like state	+	Description: mammalian 80S HCV-IRES initiation complex with eIF5B PRE-like state

OCA: Protected "4uq5" [edit=sysop:move=sysop]

OCA — Wed, 09 Jul 2014 07:02:21 GMT

Protected "4uq5" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:02, 9 July 2014

OCA: New page: '''Unreleased structure''' The entry 4uq5 is ON HOLD Authors: Yamamoto, H., Unbehaun, A., Loerke, J., Behrmann, E., Marianne, C., Burger, J., Mielke, T., Spahn, C.M.T. Description: mam...

OCA — Wed, 09 Jul 2014 07:02:20 GMT

New page: '''Unreleased structure''' The entry 4uq5 is ON HOLD Authors: Yamamoto, H., Unbehaun, A., Loerke, J., Behrmann, E., Marianne, C., Burger, J., Mielke, T., Spahn, C.M.T. Description: mam...

New page

'''Unreleased structure'''

The entry 4uq5 is ON HOLD

Authors: Yamamoto, H., Unbehaun, A., Loerke, J., Behrmann, E., Marianne, C., Burger, J., Mielke, T., Spahn, C.M.T.

Description: mammalian 80S initiation complex with eIF5B PRE-like state