4ux1 - Revision history

OCA at 07:48, 29 March 2023

OCA — Wed, 29 Mar 2023 07:48:31 GMT

←Older revision		Revision as of 07:48, 29 March 2023
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	<StructureSection load='4ux1' size='340' side='right'caption='[[4ux1]], [[Resolution\|resolution]] 8.00Å' scene=''>		<StructureSection load='4ux1' size='340' side='right'caption='[[4ux1]], [[Resolution\|resolution]] 8.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ux1]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UX1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4UX1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ux1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UX1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UX1 FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ux2\|4ux2]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ux1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ux1 OCA], [https://pdbe.org/4ux1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ux1 RCSB], [https://www.ebi.ac.uk/pdbsum/4ux1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ux1 ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrogen/potassium-exchanging_ATPase Hydrogen/potassium-exchanging ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.10 3.6.3.10] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ux1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ux1 OCA], [~~http~~://pdbe.org/4ux1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ux1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ux1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ux1 ProSAT]</span></td></tr>	+
	</table>		</table>
-	== Disease ==
-	[[http://www.uniprot.org/uniprot/ATP4B_PIG ATP4B_PIG]] Note=Parietal cell autoantigen associated with autoimmune gastritis.
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ATP4A_PIG ATP4A_PIG]] Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for acid production in the stomach.	+	[https://www.uniprot.org/uniprot/ATP4A_PIG ATP4A_PIG] Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for acid production in the stomach.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Hydrogen/potassium-exchanging ATPase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
-	[[Category: Abe, K]]	+	[[Category: Abe K]]
-	[[Category: Fujiyoshi, Y]]	+	[[Category: Fujiyoshi Y]]
-	[[Category: Tani, K]]	+	[[Category: Tani K]]
-	~~[[Category: Potassium-transporting atpase]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 07:51, 23 May 2019

OCA — Thu, 23 May 2019 07:51:18 GMT

←Older revision		Revision as of 07:51, 23 May 2019
Line 1:		Line 1:

	==Cryo-EM structure of antagonist-bound E2P gastric H,K-ATPase (SCH.E2. AlF)==		==Cryo-EM structure of antagonist-bound E2P gastric H,K-ATPase (SCH.E2. AlF)==
-	<StructureSection load='4ux1' size='340' side='right' caption='[[4ux1]], [[Resolution\|resolution]] 8.00Å' scene=''>	+	<StructureSection load='4ux1' size='340' side='right'caption='[[4ux1]], [[Resolution\|resolution]] 8.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ux1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UX1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ux1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UX1 FirstGlance]. <br>
Line 23:		Line 23:

	==See Also==		==See Also==
-	*[[ATPase\|ATPase]]	+	*[[ATPase 3D structures\|ATPase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Hydrogen/potassium-exchanging ATPase]]		[[Category: Hydrogen/potassium-exchanging ATPase]]
		+	[[Category: Large Structures]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Abe, K]]		[[Category: Abe, K]]

OCA at 01:08, 19 January 2017

OCA — Thu, 19 Jan 2017 01:08:07 GMT

←Older revision		Revision as of 01:08, 19 January 2017
Line 1:		Line 1:
		+
	==Cryo-EM structure of antagonist-bound E2P gastric H,K-ATPase (SCH.E2. AlF)==		==Cryo-EM structure of antagonist-bound E2P gastric H,K-ATPase (SCH.E2. AlF)==
	<StructureSection load='4ux1' size='340' side='right' caption='[[4ux1]], [[Resolution\|resolution]] 8.00Å' scene=''>		<StructureSection load='4ux1' size='340' side='right' caption='[[4ux1]], [[Resolution\|resolution]] 8.00Å' scene=''>
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	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ux2\|4ux2]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ux2\|4ux2]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrogen/potassium-exchanging_ATPase Hydrogen/potassium-exchanging ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.10 3.6.3.10] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrogen/potassium-exchanging_ATPase Hydrogen/potassium-exchanging ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.10 3.6.3.10] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ux1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ux1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ux1 RCSB], [http://www.ebi.ac.uk/pdbsum/4ux1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ux1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ux1 OCA], [http://pdbe.org/4ux1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ux1 RCSB], [http://www.ebi.ac.uk/pdbsum/4ux1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ux1 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ux1" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 11:45, 18 March 2015

OCA — Wed, 18 Mar 2015 11:45:48 GMT

←Older revision		Revision as of 11:45, 18 March 2015
Line 19:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[ATPase\|ATPase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:48, 12 November 2014

OCA — Wed, 12 Nov 2014 07:48:46 GMT

←Older revision		Revision as of 07:48, 12 November 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ux1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UX1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ux1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UX1 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ux2\|4ux2]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ux2\|4ux2]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrogen/potassium-exchanging_ATPase Hydrogen/potassium-exchanging ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.10 3.6.3.10] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrogen/potassium-exchanging_ATPase Hydrogen/potassium-exchanging ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.10 3.6.3.10] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ux1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ux1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ux1 RCSB], [http://www.ebi.ac.uk/pdbsum/4ux1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ux1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ux1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ux1 RCSB], [http://www.ebi.ac.uk/pdbsum/4ux1 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Disease ==		== Disease ==
	[[http://www.uniprot.org/uniprot/ATP4B_PIG ATP4B_PIG]] Note=Parietal cell autoantigen associated with autoimmune gastritis.		[[http://www.uniprot.org/uniprot/ATP4B_PIG ATP4B_PIG]] Note=Parietal cell autoantigen associated with autoimmune gastritis.
Line 25:		Line 25:
	[[Category: Hydrogen/potassium-exchanging ATPase]]		[[Category: Hydrogen/potassium-exchanging ATPase]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
-	[[Category: Abe, K.]]	+	[[Category: Abe, K]]
-	[[Category: Fujiyoshi, Y.]]	+	[[Category: Fujiyoshi, Y]]
-	[[Category: Tani, K.]]	+	[[Category: Tani, K]]
	[[Category: Potassium-transporting atpase]]		[[Category: Potassium-transporting atpase]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 21:48, 1 October 2014

OCA — Wed, 01 Oct 2014 21:48:22 GMT

←Older revision		Revision as of 21:48, 1 October 2014
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/ATP4A_PIG ATP4A_PIG]] Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for acid production in the stomach.		[[http://www.uniprot.org/uniprot/ATP4A_PIG ATP4A_PIG]] Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for acid production in the stomach.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Gastric H+,K+-ATPase, an ATP-driven proton pump responsible for the gastric acidification, is a molecular target for anti-ulcer drugs. Here we show its cryo-electron microscopy (EM) in an E2P analog state, bound to magnesium fluoride (MgF) and its K+-competitive antagonist SCH28080, determined at 7 A resolution by electron crystallography of two-dimensional crystals. Systematic comparison with other E2P-related cryo-EM structures revealed that the molecular conformation in the (SCH)E2.MgF state is remarkably distinguishable. Although the azimuthal position of the A domain of the (SCH)E2.MgF state is similar to that in the E2.AlF (aluminum fluoride) state, in which the transmembrane luminal gate is closed, the arrangement of transmembrane helices in the (SCH)E2.MgF state shows a luminal-open conformation imposed on by bound SCH28080 at its luminal cavity, based on observations of the structure in the SCH28080-bound E2.BeF (beryllium fluoride) state. The molecular conformation of the (SCH)E2.MgF state thus represents a mixed overall structure in which its cytoplasmic and luminal half appear to be independently modulated by a phosphate analog and an antagonist bound to the respective parts of the enzyme. Comparison of the molecular conformations revealed that the linker region connecting the A domain and the transmembrane helix 2 (A-M2 linker) mediates the regulation of luminal gating. The mechanistic rationale underlying luminal gating observed in H+,K+-ATPase is consistent with that observed in sarcoplasmic reticulum Ca2+-ATPase and other P-type ATPases, and is most likely conserved for the P-type ATPase family in general.
		+
		+	Systematic Comparison of Molecular Conformations of H+,K+-ATPase Reveals an Important Contribution of the A-M2 Linker for the Luminal Gating.,Abe K, Tani K, Fujiyoshi Y J Biol Chem. 2014 Sep 17. pii: jbc.M114.584623. PMID:25231997<ref>PMID:25231997</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 05:27, 24 September 2014

OCA — Wed, 24 Sep 2014 05:27:13 GMT

←Older revision		Revision as of 05:27, 24 September 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Cryo-EM structure of antagonist-bound E2P gastric H,K-ATPase (SCH.E2. AlF)==
-		+	<StructureSection load='4ux1' size='340' side='right' caption='[[4ux1]], [[Resolution\|resolution]] 8.00Å' scene=''>
-	~~The entry~~ 4ux1 is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4ux1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UX1 FirstGlance]. <br>
-	~~Authors~~: ~~Abe~~, K., ~~Tani~~, K., ~~Fujiyoshi, Y~~.	+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ux2\|4ux2]]</td></tr>
-		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrogen/potassium-exchanging_ATPase Hydrogen/potassium-exchanging ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.10 3.6.3.10] </span></td></tr>
-	~~Description~~: ~~Cryo-EM structure~~ of ~~antagonist-bound E2P gastric~~ H,K-ATPase ~~(SCH~~.E2. ~~AlF)~~	+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ux1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ux1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ux1 RCSB], [http://www.ebi.ac.uk/pdbsum/4ux1 PDBsum]</span></td></tr>
		+	<table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/ATP4B_PIG ATP4B_PIG]] Note=Parietal cell autoantigen associated with autoimmune gastritis.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ATP4A_PIG ATP4A_PIG]] Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for acid production in the stomach.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Hydrogen/potassium-exchanging ATPase]]
		+	[[Category: Sus scrofa]]
		+	[[Category: Abe, K.]]
		+	[[Category: Fujiyoshi, Y.]]
		+	[[Category: Tani, K.]]
		+	[[Category: Potassium-transporting atpase]]
		+	[[Category: Transport protein]]

OCA at 08:45, 10 September 2014

OCA — Wed, 10 Sep 2014 08:45:00 GMT

←Older revision		Revision as of 08:45, 10 September 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ux1 is ON HOLD	+	The entry 4ux1 is ON HOLD until Paper Publication

	Authors: Abe, K., Tani, K., Fujiyoshi, Y.		Authors: Abe, K., Tani, K., Fujiyoshi, Y.

	Description: Cryo-EM structure of antagonist-bound E2P gastric H,K-ATPase (SCH.E2. AlF)		Description: Cryo-EM structure of antagonist-bound E2P gastric H,K-ATPase (SCH.E2. AlF)

OCA: Protected "4ux1" [edit=sysop:move=sysop]

OCA — Wed, 27 Aug 2014 07:25:17 GMT

Protected "4ux1" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:25, 27 August 2014

OCA: New page: '''Unreleased structure''' The entry 4ux1 is ON HOLD Authors: Abe, K., Tani, K., Fujiyoshi, Y. Description: Cryo-EM structure of antagonist-bound E2P gastric H,K-ATPase (SCH.E2. AlF)

OCA — Wed, 27 Aug 2014 07:25:16 GMT

New page: '''Unreleased structure''' The entry 4ux1 is ON HOLD Authors: Abe, K., Tani, K., Fujiyoshi, Y. Description: Cryo-EM structure of antagonist-bound E2P gastric H,K-ATPase (SCH.E2. AlF)

New page

'''Unreleased structure'''

The entry 4ux1 is ON HOLD

Authors: Abe, K., Tani, K., Fujiyoshi, Y.

Description: Cryo-EM structure of antagonist-bound E2P gastric H,K-ATPase (SCH.E2. AlF)