4v4c - Revision history

OCA at 12:58, 1 March 2024

OCA — Fri, 01 Mar 2024 12:58:25 GMT

←Older revision		Revision as of 12:58, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4v4c]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Pelobacter_acidigallici Pelobacter acidigallici]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ti2 1ti2] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vld 1vld]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V4C FirstGlance]. <br>		<table><tr><td colspan='2'>[[4v4c]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Pelobacter_acidigallici Pelobacter acidigallici]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ti2 1ti2] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vld 1vld]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V4C FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4MO:MOLYBDENUM(IV)+ION'>4MO</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4MO:MOLYBDENUM(IV)+ION'>4MO</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v4c OCA], [https://pdbe.org/4v4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v4c RCSB], [https://www.ebi.ac.uk/pdbsum/4v4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v4c ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v4c OCA], [https://pdbe.org/4v4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v4c RCSB], [https://www.ebi.ac.uk/pdbsum/4v4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v4c ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/PGTL_PELAC PGTL_PELAC] Isomerisation of pyrogallol to phloroglucin.		[https://www.uniprot.org/uniprot/PGTL_PELAC PGTL_PELAC] Isomerisation of pyrogallol to phloroglucin.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The Mo enzyme transhydroxylase from the anaerobic microorganism Pelobacter acidigallici catalyzes the conversion of pyrogallol to phloroglucinol. Such trihydroxybenzenes and their derivatives represent important building blocks of plant polymers. None of the transferred hydroxyl groups originates from water during transhydroxylation; instead a cosubstrate, such as 1,2,3,5-tetrahydroxybenzene, is used in a reaction without apparent electron transfer. Here, we report on the crystal structure of the enzyme in the reduced Mo(IV) state, which we solved by single anomalous-diffraction technique. It represents the largest structure (1,149 amino acid residues per molecule, 12 independent molecules per unit cell), which has been solved so far by single anomalous-diffraction technique. Tranhydroxylase is a heterodimer, with the active Mo-molybdopterin guanine dinucleotide (MGD)(2) site in the alpha-subunit, and three [4Fe-4S] centers in the beta-subunit. The latter subunit carries a seven-stranded, mainly antiparallel beta-barrel domain. We propose a scheme for the transhydroxylation reaction based on 3D structures of complexes of the enzyme with various polyphenols serving either as substrate or inhibitor.
-
-	Crystal structure of pyrogallol-phloroglucinol transhydroxylase, an Mo enzyme capable of intermolecular hydroxyl transfer between phenols.,Messerschmidt A, Niessen H, Abt D, Einsle O, Schink B, Kroneck PM Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11571-6. Epub 2004 Jul 29. PMID:15284442<ref>PMID:15284442</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4v4c" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:03, 29 March 2023

OCA — Wed, 29 Mar 2023 08:03:16 GMT

←Older revision		Revision as of 08:03, 29 March 2023
Line 3:		Line 3:
	<StructureSection load='4v4c' size='340' side='right'caption='[[4v4c]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='4v4c' size='340' side='right'caption='[[4v4c]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4v4c]] is a 24 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Pelobacter_acidigallici Pelobacter acidigallici]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ti2 1ti2] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vld 1vld]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V4C OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4V4C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4v4c]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Pelobacter_acidigallici Pelobacter acidigallici]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ti2 1ti2] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vld 1vld]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V4C FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4MO:MOLYBDENUM(IV)+ION'>4MO</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4MO:MOLYBDENUM(IV)+ION'>4MO</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ti4\|1ti4]], [[1ti6\|1ti6]], [[1vle\|1vle]], [[1vlf\|1vlf]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v4c OCA], [https://pdbe.org/4v4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v4c RCSB], [https://www.ebi.ac.uk/pdbsum/4v4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v4c ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrogallol_hydroxytransferase Pyrogallol hydroxytransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.97.1.2 1.97.1.2] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4v4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v4c OCA], [~~http~~://pdbe.org/4v4c PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4v4c RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4v4c PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4v4c ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PGTL_PELAC PGTL_PELAC]] Isomerisation ~~of pyrogallol to phloroglucin. [[http://www.uniprot.org/uniprot/PGTS_PELAC PGTS_PELAC]] Isomerization~~ of pyrogallol to phloroglucin.	+	[https://www.uniprot.org/uniprot/PGTL_PELAC PGTL_PELAC] Isomerisation of pyrogallol to phloroglucin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 24:
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Pelobacter acidigallici]]		[[Category: Pelobacter acidigallici]]
-	~~[[Category: Pyrogallol hydroxytransferase]]~~	+	[[Category: Abt D]]
-	[[Category: Abt, D]]	+	[[Category: Einsle O]]
-	[[Category: Einsle, O]]	+	[[Category: Kroneck PMH]]
-	[[Category: Kroneck~~, P M.H~~]]	+	[[Category: Messerschmidt A]]
-	[[Category: Messerschmidt, A]]	+	[[Category: Niessen H]]
-	[[Category: Niessen, H]]	+	[[Category: Schink B]]
-	[[Category: Schink, B]]	+
-	~~[[Category: 4fe-4s-cluster]]~~	+
-	~~[[Category: Dmso-reductase family]]~~	+
-	~~[[Category: Mgd-cofactor]]~~	+
-	~~[[Category: Molybdenum binding enzyme]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 08:26, 23 May 2019

OCA — Thu, 23 May 2019 08:26:18 GMT

←Older revision		Revision as of 08:26, 23 May 2019
Line 1:		Line 1:
		+
	==Crystal Structure of Pyrogallol-Phloroglucinol Transhydroxylase from Pelobacter acidigallici==		==Crystal Structure of Pyrogallol-Phloroglucinol Transhydroxylase from Pelobacter acidigallici==
-	<StructureSection load='4v4c' size='340' side='right' caption='[[4v4c]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='4v4c' size='340' side='right'caption='[[4v4c]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4v4c]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Pelobacter_acidigallici Pelobacter acidigallici]. This structure supersedes ~~and combines~~ the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=~~1vld 1vld~~] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=~~1ti2 1ti2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4V4C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4v4c]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Pelobacter_acidigallici Pelobacter acidigallici]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ti2 1ti2] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vld 1vld]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4V4C FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4MO:MOLYBDENUM(IV)+ION'>4MO</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4MO:MOLYBDENUM(IV)+ION'>4MO</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ti4\|1ti4]], [[1ti6\|1ti6]], [[1vle\|1vle]], [[1vlf\|1vlf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ti4\|1ti4]], [[1ti6\|1ti6]], [[1vle\|1vle]], [[1vlf\|1vlf]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrogallol_hydroxytransferase Pyrogallol hydroxytransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.97.1.2 1.97.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrogallol_hydroxytransferase Pyrogallol hydroxytransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.97.1.2 1.97.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v4c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4v4c RCSB], [http://www.ebi.ac.uk/pdbsum/4v4c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v4c OCA], [http://pdbe.org/4v4c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4v4c RCSB], [http://www.ebi.ac.uk/pdbsum/4v4c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4v4c ProSAT]</span></td></tr>
	</table>		</table>
-	{{Large structure}}
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/PGTL_PELAC PGTL_PELAC]] Isomerisation of pyrogallol to phloroglucin. [[http://www.uniprot.org/uniprot/PGTS_PELAC PGTS_PELAC]] Isomerization of pyrogallol to phloroglucin.		[[http://www.uniprot.org/uniprot/PGTL_PELAC PGTL_PELAC]] Isomerisation of pyrogallol to phloroglucin. [[http://www.uniprot.org/uniprot/PGTS_PELAC PGTS_PELAC]] Isomerization of pyrogallol to phloroglucin.
Line 19:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4v4c" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Pelobacter acidigallici]]		[[Category: Pelobacter acidigallici]]
	[[Category: Pyrogallol hydroxytransferase]]		[[Category: Pyrogallol hydroxytransferase]]

OCA at 16:13, 1 September 2015

OCA — Tue, 01 Sep 2015 16:13:49 GMT

←Older revision		Revision as of 16:13, 1 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[4v4c]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Pelobacter_acidigallici Pelobacter acidigallici]. This structure supersedes and combines the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vld 1vld] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ti2 1ti2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4V4C FirstGlance]. <br>		<table><tr><td colspan='2'>[[4v4c]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Pelobacter_acidigallici Pelobacter acidigallici]. This structure supersedes and combines the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vld 1vld] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ti2 1ti2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4V4C FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4MO:MOLYBDENUM(IV)+ION'>4MO</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4MO:MOLYBDENUM(IV)+ION'>4MO</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">~~[[1vld\|1vld]], [[1ti2\|1ti2]],~~ [[1ti4\|1ti4]], [[1ti6\|1ti6]], [[1vle\|1vle]], [[1vlf\|1vlf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ti4\|1ti4]], [[1ti6\|1ti6]], [[1vle\|1vle]], [[1vlf\|1vlf]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrogallol_hydroxytransferase Pyrogallol hydroxytransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.97.1.2 1.97.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrogallol_hydroxytransferase Pyrogallol hydroxytransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.97.1.2 1.97.1.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v4c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4v4c RCSB], [http://www.ebi.ac.uk/pdbsum/4v4c PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v4c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4v4c RCSB], [http://www.ebi.ac.uk/pdbsum/4v4c PDBsum]</span></td></tr>

OCA at 13:23, 1 September 2015

OCA — Tue, 01 Sep 2015 13:23:30 GMT

←Older revision		Revision as of 13:23, 1 September 2015
Line 2:		Line 2:
	<StructureSection load='4v4c' size='340' side='right' caption='[[4v4c]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='4v4c' size='340' side='right' caption='[[4v4c]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4v4c]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Pelobacter_acidigallici Pelobacter acidigallici]. This structure supersedes the now removed PDB entries ~~and~~ [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vld 1vld]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4V4C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4v4c]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Pelobacter_acidigallici Pelobacter acidigallici]. This structure supersedes and combines the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vld 1vld] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ti2 1ti2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4V4C FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4MO:MOLYBDENUM(IV)+ION'>4MO</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4MO:MOLYBDENUM(IV)+ION'>4MO</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vld\|1vld]], [[1ti2\|1ti2]], [[1ti4\|1ti4]], [[1ti6\|1ti6]], [[1vle\|1vle]], [[1vlf\|1vlf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vld\|1vld]], [[1ti2\|1ti2]], [[1ti4\|1ti4]], [[1ti6\|1ti6]], [[1vle\|1vle]], [[1vlf\|1vlf]]</td></tr>
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v4c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4v4c RCSB], [http://www.ebi.ac.uk/pdbsum/4v4c PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v4c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4v4c RCSB], [http://www.ebi.ac.uk/pdbsum/4v4c PDBsum]</span></td></tr>
	</table>		</table>
		+	{{Large structure}}
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/PGTL_PELAC PGTL_PELAC]] Isomerisation of pyrogallol to phloroglucin. [[http://www.uniprot.org/uniprot/PGTS_PELAC PGTS_PELAC]] Isomerization of pyrogallol to phloroglucin.		[[http://www.uniprot.org/uniprot/PGTL_PELAC PGTL_PELAC]] Isomerisation of pyrogallol to phloroglucin. [[http://www.uniprot.org/uniprot/PGTS_PELAC PGTS_PELAC]] Isomerization of pyrogallol to phloroglucin.

OCA: Protected "4v4c" [edit=sysop:move=sysop]

OCA — Thu, 25 Dec 2014 17:15:02 GMT

Protected "4v4c" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:15, 25 December 2014

OCA: New page: ==Crystal Structure of Pyrogallol-Phloroglucinol Transhydroxylase from Pelobacter acidigallici== == Structural highlights ==
<table><tr><td colspan='2'>[[4v4c]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Pelobacter_acidigallici Pelobacter acidigallici]. This structure supersedes the now removed PDB entries and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vld 1vld]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4V4C FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4MO:MOLYBDENUM(IV)+ION'>4MO</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1vld|1vld]], [[1ti2|1ti2]], [[1ti4|1ti4]], [[1ti6|1ti6]], [[1vle|1vle]], [[1vlf|1vlf]]</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrogallol_hydroxytransferase Pyrogallol hydroxytransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.97.1.2 1.97.1.2] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v4c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4v4c RCSB], [http://www.ebi.ac.uk/pdbsum/4v4c PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/PGTL_PELAC PGTL_PELAC]] Isomerisation of pyrogallol to phloroglucin. [[http://www.uniprot.org/uniprot/PGTS_PELAC PGTS_PELAC]] Isomerization of pyrogallol to phloroglucin.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Mo enzyme transhydroxylase from the anaerobic microorganism Pelobacter acidigallici catalyzes the conversion of pyrogallol to phloroglucinol. Such trihydroxybenzenes and their derivatives represent important building blocks of plant polymers. None of the transferred hydroxyl groups originates from water during transhydroxylation; instead a cosubstrate, such as 1,2,3,5-tetrahydroxybenzene, is used in a reaction without apparent electron transfer. Here, we report on the crystal structure of the enzyme in the reduced Mo(IV) state, which we solved by single anomalous-diffraction technique. It represents the largest structure (1,149 amino acid residues per molecule, 12 independent molecules per unit cell), which has been solved so far by single anomalous-diffraction technique. Tranhydroxylase is a heterodimer, with the active Mo-molybdopterin guanine dinucleotide (MGD)(2) site in the alpha-subunit, and three [4Fe-4S] centers in the beta-subunit. The latter subunit carries a seven-stranded, mainly antiparallel beta-barrel domain. We propose a scheme for the transhydroxylation reaction based on 3D structures of complexes of the enzyme with various polyphenols serving either as substrate or inhibitor.

Crystal structure of pyrogallol-phloroglucinol transhydroxylase, an Mo enzyme capable of intermolecular hydroxyl transfer between phenols.,Messerschmidt A, Niessen H, Abt D, Einsle O, Schink B, Kroneck PM Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11571-6. Epub 2004 Jul 29. PMID:15284442<ref>PMID:15284442</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Pelobacter acidigallici]]
[[Category: Pyrogallol hydroxytransferase]]
[[Category: Abt, D]]
[[Category: Einsle, O]]
[[Category: Kroneck, P M.H]]
[[Category: Messerschmidt, A]]
[[Category: Niessen, H]]
[[Category: Schink, B]]
[[Category: 4fe-4s-cluster]]
[[Category: Dmso-reductase family]]
[[Category: Mgd-cofactor]]
[[Category: Molybdenum binding enzyme]]
[[Category: Oxidoreductase]]