4w6w - Revision history

OCA at 10:40, 10 January 2024

OCA — Wed, 10 Jan 2024 10:40:55 GMT

←Older revision		Revision as of 10:40, 10 January 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4w6w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4W6W FirstGlance]. <br>		<table><tr><td colspan='2'>[[4w6w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4W6W FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4w6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w6w OCA], [https://pdbe.org/4w6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4w6w RCSB], [https://www.ebi.ac.uk/pdbsum/4w6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4w6w ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.51Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4w6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w6w OCA], [https://pdbe.org/4w6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4w6w RCSB], [https://www.ebi.ac.uk/pdbsum/4w6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4w6w ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 06:55, 7 April 2023

OCA — Fri, 07 Apr 2023 06:55:28 GMT

←Older revision		Revision as of 06:55, 7 April 2023
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	==Co-complex structure of the lectin domain of F18 fimbrial adhesin FedF with inhibitory nanobody NbFedF6==		==Co-complex structure of the lectin domain of F18 fimbrial adhesin FedF with inhibitory nanobody NbFedF6==
-	<StructureSection load='4w6w' size='340' side='right' caption='[[4w6w]], [[Resolution\|resolution]] 2.51Å' scene=''>	+	<StructureSection load='4w6w' size='340' side='right'caption='[[4w6w]], [[Resolution\|resolution]] 2.51Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4w6w]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Camelus_glama Camelus glama~~] and [~~http~~://en.wikipedia.org/wiki/~~Escherichia_coli_dh5[alpha] Escherichia coli dh5[alpha]~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W6W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4W6W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4w6w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4W6W FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4b4p\|4b4p]], [[4w6x\|4w6x]], [[4w6y\|4w6y]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4w6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w6w OCA], [https://pdbe.org/4w6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4w6w RCSB], [https://www.ebi.ac.uk/pdbsum/4w6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4w6w ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fedF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=668369 Escherichia coli DH5[alpha]])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4w6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w6w OCA], [~~http~~://pdbe.org/4w6w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4w6w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4w6w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4w6w ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q47212_ECOLX Q47212_ECOLX]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4w6w" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4w6w" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Adhesin 3D structures\|Adhesin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Camelus glama~~]]	+	[[Category: Escherichia coli]]
-	[[Category: ~~Annelies, C~~]]	+	[[Category: Lama glama]]
-	[[Category: ~~Cox, E~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Greve, H De~~]]	+	[[Category: Annelies C]]
-	[[Category: ~~Kerpel, M De]]~~	+	[[Category: Cox E]]
-	~~[[Category: Moonens, K]]~~	+	[[Category: De Greve H]]
-	~~[[Category: Pardon,~~ E]]	+	[[Category: De Kerpel M]]
-	[[Category: ~~Remaut,~~ H]]	+	[[Category: Moonens K]]
-	[[Category: ~~Adhesin~~]]	+	[[Category: Pardon E]]
-	[[Category: ~~Cell adhesion~~]]	+	[[Category: Remaut H]]
-	[[Category: ~~Fimbriae~~]]	+
-	[[Category: ~~Inhibitor~~]]	+

OCA at 06:36, 7 February 2018

OCA — Wed, 07 Feb 2018 06:36:52 GMT

←Older revision		Revision as of 06:36, 7 February 2018
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	<StructureSection load='4w6w' size='340' side='right' caption='[[4w6w]], [[Resolution\|resolution]] 2.51Å' scene=''>		<StructureSection load='4w6w' size='340' side='right' caption='[[4w6w]], [[Resolution\|resolution]] 2.51Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4w6w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W6W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W6W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4w6w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama] and [http://en.wikipedia.org/wiki/Escherichia_coli_dh5[alpha] Escherichia coli dh5[alpha]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W6W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W6W FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4b4p\|4b4p]], [[4w6x\|4w6x]], [[4w6y\|4w6y]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4b4p\|4b4p]], [[4w6x\|4w6x]], [[4w6y\|4w6y]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fedF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=668369 Escherichia coli DH5[alpha]])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w6w OCA], [http://pdbe.org/4w6w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4w6w RCSB], [http://www.ebi.ac.uk/pdbsum/4w6w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4w6w ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w6w OCA], [http://pdbe.org/4w6w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4w6w RCSB], [http://www.ebi.ac.uk/pdbsum/4w6w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4w6w ProSAT]</span></td></tr>
	</table>		</table>
Line 16:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 4w6w" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4w6w" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Adhesin\|Adhesin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Camelus glama]]
	[[Category: Annelies, C]]		[[Category: Annelies, C]]
	[[Category: Cox, E]]		[[Category: Cox, E]]

OCA at 09:34, 4 August 2016

OCA — Thu, 04 Aug 2016 09:34:29 GMT

←Older revision		Revision as of 09:34, 4 August 2016
Line 1:		Line 1:
		+
	==Co-complex structure of the lectin domain of F18 fimbrial adhesin FedF with inhibitory nanobody NbFedF6==		==Co-complex structure of the lectin domain of F18 fimbrial adhesin FedF with inhibitory nanobody NbFedF6==
	<StructureSection load='4w6w' size='340' side='right' caption='[[4w6w]], [[Resolution\|resolution]] 2.51Å' scene=''>		<StructureSection load='4w6w' size='340' side='right' caption='[[4w6w]], [[Resolution\|resolution]] 2.51Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4w6w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W6W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W6W FirstGlance]. <br>		<table><tr><td colspan='2'>[[4w6w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W6W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W6W FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4b4p\|4b4p]], [[4w6x\|4w6x]], [[4w6y\|4w6y]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4b4p\|4b4p]], [[4w6x\|4w6x]], [[4w6y\|4w6y]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w6w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4w6w RCSB], [http://www.ebi.ac.uk/pdbsum/4w6w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w6w OCA], [http://pdbe.org/4w6w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4w6w RCSB], [http://www.ebi.ac.uk/pdbsum/4w6w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4w6w ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4w6w" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Adhesin\|Adhesin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:56, 24 December 2014

OCA — Wed, 24 Dec 2014 10:56:12 GMT

←Older revision		Revision as of 10:56, 24 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w6w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4w6w RCSB], [http://www.ebi.ac.uk/pdbsum/4w6w PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w6w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4w6w RCSB], [http://www.ebi.ac.uk/pdbsum/4w6w PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Post-weaning diarrhea and edema disease caused by F18 fimbriated E. coli are important diseases in newly weaned piglets and lead to severe production losses in farming industry. Protective treatments against these infections have thus far limited efficacy. In this study we generated nanobodies directed against the lectin domain of the F18 fimbrial adhesin FedF and showed in an in vitro adherence assay that four unique nanobodies inhibit the attachment of F18 fimbriated E. coli bacteria to piglet enterocytes. Crystallization of the FedF lectin domain with the most potent inhibitory nanobodies revealed their mechanism of action. These either competed with the binding of the blood group antigen receptor on the FedF surface or induced a conformational change in which the CDR3 region of the nanobody displaces the D''-E loop adjacent to the binding site. This D''-E loop was previously shown to be required for the interaction between F18 fimbriated bacteria and blood group antigen receptors in a membrane context. This work demonstrates the feasibility of inhibiting the attachment of fimbriated pathogens by employing nanobodies directed against the adhesin domain.
		+
		+	Nanobody Mediated Inhibition of Attachment of F18 Fimbriae Expressing Escherichia coli.,Moonens K, De Kerpel M, Coddens A, Cox E, Pardon E, Remaut H, De Greve H PLoS One. 2014 Dec 11;9(12):e114691. doi: 10.1371/journal.pone.0114691., eCollection 2014. PMID:25502211<ref>PMID:25502211</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:03, 17 December 2014

OCA — Wed, 17 Dec 2014 13:03:24 GMT

←Older revision		Revision as of 13:03, 17 December 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Co-complex structure of the lectin domain of F18 fimbrial adhesin FedF with inhibitory nanobody NbFedF6==
-		+	<StructureSection load='4w6w' size='340' side='right' caption='[[4w6w]], [[Resolution\|resolution]] 2.51Å' scene=''>
-	~~The entry~~ 4w6w is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4w6w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W6W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W6W FirstGlance]. <br>
-	~~Authors~~: ~~Moonens~~, K., ~~De Kerpel, M~~., Annelies, C., Cox, E., Pardon, E., Remaut, H~~., De Greve, H.~~	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4b4p\|4b4p]], [[4w6x\|4w6x]], [[4w6y\|4w6y]]</td></tr>
-		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w6w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4w6w RCSB], [http://www.ebi.ac.uk/pdbsum/4w6w PDBsum]</span></td></tr>
-	~~Description~~: ~~Co-complex structure of the lectin domain of F18 fimbrial adhesin FedF with inhibitory nanobody NbFedF6~~	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Annelies, C]]
		+	[[Category: Cox, E]]
		+	[[Category: Greve, H De]]
		+	[[Category: Kerpel, M De]]
		+	[[Category: Moonens, K]]
		+	[[Category: Pardon, E]]
		+	[[Category: Remaut, H]]
		+	[[Category: Adhesin]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Fimbriae]]
		+	[[Category: Inhibitor]]

OCA at 04:45, 4 September 2014

OCA — Thu, 04 Sep 2014 04:45:53 GMT

←Older revision		Revision as of 04:45, 4 September 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4w6w is ON HOLD	+	The entry 4w6w is ON HOLD until Paper Publication

	Authors: Moonens, K., De Kerpel, M., Annelies, C., Cox, E., Pardon, E., Remaut, H., De Greve, H.		Authors: Moonens, K., De Kerpel, M., Annelies, C., Cox, E., Pardon, E., Remaut, H., De Greve, H.

	Description: Co-complex structure of the lectin domain of F18 fimbrial adhesin FedF with inhibitory nanobody NbFedF6		Description: Co-complex structure of the lectin domain of F18 fimbrial adhesin FedF with inhibitory nanobody NbFedF6

OCA: Protected "4w6w" [edit=sysop:move=sysop]

OCA — Wed, 27 Aug 2014 07:31:17 GMT

Protected "4w6w" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:31, 27 August 2014

OCA: New page: '''Unreleased structure''' The entry 4w6w is ON HOLD Authors: Moonens, K., De Kerpel, M., Annelies, C., Cox, E., Pardon, E., Remaut, H., De Greve, H. Description: Co-complex structure ...

OCA — Wed, 27 Aug 2014 07:31:15 GMT

New page: '''Unreleased structure''' The entry 4w6w is ON HOLD Authors: Moonens, K., De Kerpel, M., Annelies, C., Cox, E., Pardon, E., Remaut, H., De Greve, H. Description: Co-complex structure ...

New page

'''Unreleased structure'''

The entry 4w6w is ON HOLD

Authors: Moonens, K., De Kerpel, M., Annelies, C., Cox, E., Pardon, E., Remaut, H., De Greve, H.

Description: Co-complex structure of the lectin domain of F18 fimbrial adhesin FedF with inhibitory nanobody NbFedF6