4w81 - Revision history

OCA at 07:29, 27 September 2023

OCA — Wed, 27 Sep 2023 07:29:04 GMT

←Older revision		Revision as of 07:29, 27 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4w81]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4W81 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4w81]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4W81 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.25Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4w81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w81 OCA], [https://pdbe.org/4w81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4w81 RCSB], [https://www.ebi.ac.uk/pdbsum/4w81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4w81 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4w81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w81 OCA], [https://pdbe.org/4w81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4w81 RCSB], [https://www.ebi.ac.uk/pdbsum/4w81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4w81 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:56, 7 April 2023

OCA — Fri, 07 Apr 2023 06:56:39 GMT

←Older revision		Revision as of 06:56, 7 April 2023
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	<StructureSection load='4w81' size='340' side='right'caption='[[4w81]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='4w81' size='340' side='right'caption='[[4w81]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4w81]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Camelus_glama Camelus~~ glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W81 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4W81 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4w81]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4W81 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4w68\|4w68]], [[4w70\|4w70]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4w81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w81 OCA], [https://pdbe.org/4w81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4w81 RCSB], [https://www.ebi.ac.uk/pdbsum/4w81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4w81 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4w81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w81 OCA], [~~http~~://pdbe.org/4w81 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4w81 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4w81 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4w81 ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
	*[[Antibody 3D structures\|Antibody 3D structures]]		*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[3D structures of non-human antibody\|3D structures of non-human antibody]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Camelus~~ glama]]	+	[[Category: Lama glama]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: George, J]]	+	[[Category: George J]]
-	[[Category: Legler~~, P M]]~~	+	[[Category: Legler PM]]
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Single domain antibody]]~~	+
-	~~[[Category: V-set domain~~]]	+

OCA at 08:31, 23 May 2019

OCA — Thu, 23 May 2019 08:31:27 GMT

←Older revision		Revision as of 08:31, 23 May 2019
Line 1:		Line 1:

	==Periplasmically Produced Monomeric Single Domain Antibody (sdAb) C22A/C99V variant against Staphylococcal enterotoxin B (SEB) at pH 8.0==		==Periplasmically Produced Monomeric Single Domain Antibody (sdAb) C22A/C99V variant against Staphylococcal enterotoxin B (SEB) at pH 8.0==
-	<StructureSection load='4w81' size='340' side='right' caption='[[4w81]], [[Resolution\|resolution]] 2.25Å' scene=''>	+	<StructureSection load='4w81' size='340' side='right'caption='[[4w81]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4w81]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W81 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W81 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4w81]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W81 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W81 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4w68\|4w68]], [[4w70\|4w70]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4w68\|4w68]], [[4w70\|4w70]]</td></tr>
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	</div>		</div>
	<div class="pdbe-citations 4w81" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4w81" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Camelus glama]]
		+	[[Category: Large Structures]]
	[[Category: George, J]]		[[Category: George, J]]
	[[Category: Legler, P M]]		[[Category: Legler, P M]]
		+	[[Category: Immune system]]
	[[Category: Single domain antibody]]		[[Category: Single domain antibody]]
	[[Category: V-set domain]]		[[Category: V-set domain]]

OCA at 10:14, 11 August 2016

OCA — Thu, 11 Aug 2016 10:14:08 GMT

←Older revision		Revision as of 10:14, 11 August 2016
Line 1:		Line 1:
		+
	==Periplasmically Produced Monomeric Single Domain Antibody (sdAb) C22A/C99V variant against Staphylococcal enterotoxin B (SEB) at pH 8.0==		==Periplasmically Produced Monomeric Single Domain Antibody (sdAb) C22A/C99V variant against Staphylococcal enterotoxin B (SEB) at pH 8.0==
	<StructureSection load='4w81' size='340' side='right' caption='[[4w81]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='4w81' size='340' side='right' caption='[[4w81]], [[Resolution\|resolution]] 2.25Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4w68\|4w68]], [[4w70\|4w70]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4w68\|4w68]], [[4w70\|4w70]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w81 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4w81 RCSB], [http://www.ebi.ac.uk/pdbsum/4w81 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w81 OCA], [http://pdbe.org/4w81 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4w81 RCSB], [http://www.ebi.ac.uk/pdbsum/4w81 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4w81 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4w81" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:05, 25 January 2015

OCA — Sun, 25 Jan 2015 11:05:23 GMT

←Older revision		Revision as of 11:05, 25 January 2015
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: George, J.]]	+	[[Category: George, J]]
-	[[Category: Legler, P M.]]	+	[[Category: Legler, P M]]
	[[Category: Single domain antibody]]		[[Category: Single domain antibody]]
	[[Category: V-set domain]]		[[Category: V-set domain]]

OCA at 08:50, 5 November 2014

OCA — Wed, 05 Nov 2014 08:50:16 GMT

←Older revision		Revision as of 08:50, 5 November 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Periplasmically Produced Monomeric Single Domain Antibody (sdAb) C22A/C99V variant against Staphylococcal enterotoxin B (SEB) at pH 8.0==
		+	<StructureSection load='4w81' size='340' side='right' caption='[[4w81]], [[Resolution\|resolution]] 2.25Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4w81]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W81 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W81 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4w68\|4w68]], [[4w70\|4w70]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w81 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4w81 RCSB], [http://www.ebi.ac.uk/pdbsum/4w81 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Camelid single domain antibodies (sdAb) are known for their thermal stability and reversible refolding. We have characterized an unusually stable sdAb recognizing Staphylococcal enterotoxin B with one of the highest reported melting temperatures (Tm = 85 degrees C). Unexpectedly, ~10-20% of the protein formed a dimer in solution. Three other cases where <20% of the sdAb dimerized have been reported; however, this is the first report of both the monomeric and dimeric X-ray crystal structures. Concentration of the monomer did not lead to the formation of new dimer suggesting a stable conformationally distinct species in a fraction of the cytoplasmically expressed protein. Comparison of periplasmic and cytoplasmic expression showed that the dimer was associated with cytoplasmic expression. The disulfide bond was partially reduced in the WT protein purified from the cytoplasm and the protein irreversibly unfolded. Periplasmic expression produced monomeric protein with a fully formed disulfide bond and mostly reversible refolding. Crystallization of a disulfide-bond free variant, C22A/C99A, purified from the periplasm yielded a structure of a monomeric form, while crystallization of C22A/C99A from the cytoplasm produced an asymmetric dimer. In the dimer, a significant conformational asymmetry was found in the loop residues of the edge beta-strands (S50-Y60) containing the highly variable complementarity determining region, CDR2. Two dimeric assemblies were predicted from the crystal packing. Mutation of a residue at one of the interfaces, Y98A, disrupted the dimer in solution. The pleomorphic homodimer may yield insight into the stability of misfolded states and the importance of the conserved disulfide bond in preventing their formation. (c) Proteins 2014;. (c) 2014 Wiley Periodicals, Inc.

-	~~The entry 4w81 is ON HOLD~~	+	Structural and mutational analysis of a monomeric and dimeric form of a single domain antibody with implications for protein misfolding.,George J, Compton JR, Leary DH, Olson MA, Legler PM Proteins. 2014 Aug 18. doi: 10.1002/prot.24671. PMID:25136772<ref>PMID:25136772</ref>

-	~~Authors~~: George, J., Legler, P.M.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Periplasmically Produced Monomeric~~ Single ~~Domain Antibody (sdAb) C22A/C99V variant against Staphylococcal enterotoxin B (SEB) at pH 8.0~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: George, J.]]
		+	[[Category: Legler, P M.]]
		+	[[Category: Single domain antibody]]
		+	[[Category: V-set domain]]

OCA: Protected "4w81" [edit=sysop:move=sysop]

OCA — Thu, 04 Sep 2014 04:47:24 GMT

Protected "4w81" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:47, 4 September 2014

OCA: New page: '''Unreleased structure''' The entry 4w81 is ON HOLD Authors: George, J., Legler, P.M. Description: Periplasmically Produced Monomeric Single Domain Antibody (sdAb) C22A/C99V variant a...

OCA — Thu, 04 Sep 2014 04:47:22 GMT

New page: '''Unreleased structure''' The entry 4w81 is ON HOLD Authors: George, J., Legler, P.M. Description: Periplasmically Produced Monomeric Single Domain Antibody (sdAb) C22A/C99V variant a...

New page

'''Unreleased structure'''

The entry 4w81 is ON HOLD

Authors: George, J., Legler, P.M.

Description: Periplasmically Produced Monomeric Single Domain Antibody (sdAb) C22A/C99V variant against Staphylococcal enterotoxin B (SEB) at pH 8.0