4w8b - Revision history

OCA at 00:51, 28 December 2023

OCA — Thu, 28 Dec 2023 00:51:51 GMT

←Older revision		Revision as of 00:51, 28 December 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4w8b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_bacterium Uncultured bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W8B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4W8B FirstGlance]. <br>		<table><tr><td colspan='2'>[[4w8b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_bacterium Uncultured bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W8B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4W8B FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.15Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4w8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8b OCA], [https://pdbe.org/4w8b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4w8b RCSB], [https://www.ebi.ac.uk/pdbsum/4w8b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4w8b ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4w8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8b OCA], [https://pdbe.org/4w8b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4w8b RCSB], [https://www.ebi.ac.uk/pdbsum/4w8b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4w8b ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:56, 7 April 2023

OCA — Fri, 07 Apr 2023 06:56:53 GMT

←Older revision		Revision as of 06:56, 7 April 2023
Line 3:		Line 3:
	<StructureSection load='4w8b' size='340' side='right'caption='[[4w8b]], [[Resolution\|resolution]] 1.15Å' scene=''>		<StructureSection load='4w8b' size='340' side='right'caption='[[4w8b]], [[Resolution\|resolution]] 1.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4w8b]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Uncultivated_bacterium Uncultivated~~ bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W8B OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4W8B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4w8b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_bacterium Uncultured bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W8B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4W8B FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4w84\|4w84]], [[4w85\|4w85]], [[4w86\|4w86]], [[4w87\|4w87]], [[4w88\|4w88]], [[4w89\|4w89]], [[4w8a\|4w8a]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4w8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8b OCA], [https://pdbe.org/4w8b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4w8b RCSB], [https://www.ebi.ac.uk/pdbsum/4w8b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4w8b ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4w8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8b OCA], [~~http~~://pdbe.org/4w8b PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4w8b RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4w8b PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4w8b ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/D2XML9_9BACT D2XML9_9BACT]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4w8b" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4w8b" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glucanase 3D structures\|Glucanase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Uncultivated~~ bacterium]]	+	[[Category: Uncultured bacterium]]
-	[[Category: Cordeiro~~, R L~~]]	+	[[Category: Cordeiro RL]]
-	[[Category: Murakami~~, M T~~]]	+	[[Category: Murakami MT]]
-	[[Category: Santos~~, C R~~]]	+	[[Category: Santos CR]]
-	[[Category: Wong~~, D W.S]]~~	+	[[Category: Wong DWS]]
-	~~[[Category: Cell wall degrading enzyme]]~~	+
-	~~[[Category: Gh5 family]]~~	+
-	~~[[Category: Glycoside hydrolase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 06:18, 17 April 2019

OCA — Wed, 17 Apr 2019 06:18:07 GMT

←Older revision		Revision as of 06:18, 17 April 2019
Line 1:		Line 1:

	==Crystal structure of XEG5B, a GH5 xyloglucan-specific beta-1,4-glucanase from ruminal metagenomic library, in complex with XXLG==		==Crystal structure of XEG5B, a GH5 xyloglucan-specific beta-1,4-glucanase from ruminal metagenomic library, in complex with XXLG==
-	<StructureSection load='4w8b' size='340' side='right' caption='[[4w8b]], [[Resolution\|resolution]] 1.15Å' scene=''>	+	<StructureSection load='4w8b' size='340' side='right'caption='[[4w8b]], [[Resolution\|resolution]] 1.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4w8b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Uncultivated_bacterium Uncultivated bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W8B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W8B FirstGlance]. <br>		<table><tr><td colspan='2'>[[4w8b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Uncultivated_bacterium Uncultivated bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W8B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W8B FirstGlance]. <br>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Uncultivated bacterium]]		[[Category: Uncultivated bacterium]]
	[[Category: Cordeiro, R L]]		[[Category: Cordeiro, R L]]

OCA at 09:24, 22 November 2017

OCA — Wed, 22 Nov 2017 09:24:08 GMT

←Older revision		Revision as of 09:24, 22 November 2017
Line 3:		Line 3:
	<StructureSection load='4w8b' size='340' side='right' caption='[[4w8b]], [[Resolution\|resolution]] 1.15Å' scene=''>		<StructureSection load='4w8b' size='340' side='right' caption='[[4w8b]], [[Resolution\|resolution]] 1.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4w8b]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W8B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W8B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4w8b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Uncultivated_bacterium Uncultivated bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W8B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W8B FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=~~GLC~~:~~ALPHA~~-D-~~GLUCOSE~~'>~~GLC~~</scene>, <scene name='pdbligand=~~GOL~~:~~GLYCEROL~~'>~~GOL~~</scene>, <scene name='pdbligand=~~SO4~~:~~SULFATE+ION~~'>~~SO4~~</scene>, <scene name='pdbligand=~~XYS~~:~~XYLOPYRANOSE~~'>~~XYS~~</scene>, <scene name='pdbligand=~~GAL~~:~~BETA-D-GALACTOSE~~'>~~GAL~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4w84\|4w84]], [[4w85\|4w85]], [[4w86\|4w86]], [[4w87\|4w87]], [[4w88\|4w88]], [[4w89\|4w89]], [[4w8a\|4w8a]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4w84\|4w84]], [[4w85\|4w85]], [[4w86\|4w86]], [[4w87\|4w87]], [[4w88\|4w88]], [[4w89\|4w89]], [[4w8a\|4w8a]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8b OCA], [http://pdbe.org/4w8b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4w8b RCSB], [http://www.ebi.ac.uk/pdbsum/4w8b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4w8b ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8b OCA], [http://pdbe.org/4w8b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4w8b RCSB], [http://www.ebi.ac.uk/pdbsum/4w8b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4w8b ProSAT]</span></td></tr>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Uncultivated bacterium]]
	[[Category: Cordeiro, R L]]		[[Category: Cordeiro, R L]]
	[[Category: Murakami, M T]]		[[Category: Murakami, M T]]
Line 28:		Line 29:
	[[Category: Gh5 family]]		[[Category: Gh5 family]]
	[[Category: Glycoside hydrolase]]		[[Category: Glycoside hydrolase]]
		+	[[Category: Hydrolase]]

OCA at 01:19, 19 January 2017

OCA — Thu, 19 Jan 2017 01:19:38 GMT

←Older revision		Revision as of 01:19, 19 January 2017
Line 1:		Line 1:
		+
	==Crystal structure of XEG5B, a GH5 xyloglucan-specific beta-1,4-glucanase from ruminal metagenomic library, in complex with XXLG==		==Crystal structure of XEG5B, a GH5 xyloglucan-specific beta-1,4-glucanase from ruminal metagenomic library, in complex with XXLG==
	<StructureSection load='4w8b' size='340' side='right' caption='[[4w8b]], [[Resolution\|resolution]] 1.15Å' scene=''>		<StructureSection load='4w8b' size='340' side='right' caption='[[4w8b]], [[Resolution\|resolution]] 1.15Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4w84\|4w84]], [[4w85\|4w85]], [[4w86\|4w86]], [[4w87\|4w87]], [[4w88\|4w88]], [[4w89\|4w89]], [[4w8a\|4w8a]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4w84\|4w84]], [[4w85\|4w85]], [[4w86\|4w86]], [[4w87\|4w87]], [[4w88\|4w88]], [[4w89\|4w89]], [[4w8a\|4w8a]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4w8b RCSB], [http://www.ebi.ac.uk/pdbsum/4w8b PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8b OCA], [http://pdbe.org/4w8b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4w8b RCSB], [http://www.ebi.ac.uk/pdbsum/4w8b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4w8b ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4w8b" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:47, 11 March 2015

OCA — Wed, 11 Mar 2015 11:47:01 GMT

←Older revision		Revision as of 11:47, 11 March 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of XEG5B, a GH5 xyloglucan-specific beta-1,4-glucanase from ruminal metagenomic library, in complex with XXLG==
		+	<StructureSection load='4w8b' size='340' side='right' caption='[[4w8b]], [[Resolution\|resolution]] 1.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4w8b]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W8B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W8B FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4w84\|4w84]], [[4w85\|4w85]], [[4w86\|4w86]], [[4w87\|4w87]], [[4w88\|4w88]], [[4w89\|4w89]], [[4w8a\|4w8a]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4w8b RCSB], [http://www.ebi.ac.uk/pdbsum/4w8b PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	GH5 is one of the largest glycoside hydrolase families, comprising at least 20 distinct activities within a common structural scaffold. However, the molecular basis for the functional differentiation among GH5 members is still not fully understood, principally for xyloglucan specificity. In this work, we elucidated the crystal structures of two novel GH5 xyloglucanases (XEGs) retrieved from a rumen microflora metagenomic library, in the native state and in complex with xyloglucan-derived oligosaccharides. These results provided insights into the structural determinants that differentiate GH5 XEGs from parental cellulases and a new mode of action within the GH5 family related to structural adaptations in the -1 subsite. The oligosaccharide found in the XEG5A complex, permitted the mapping, for the first time, of the positive subsites of a GH5 XEG, revealing the importance of the pocket-like topology of the +1 subsite in conferring the ability of some GH5 enzymes to attack xyloglucan. Complementarily, the XEG5B complex covered the negative subsites, completing the subsite mapping of GH5 XEGs at high resolution. Interestingly, XEG5B is, to date, the only GH5 member able to cleave XXXG into XX and XG, and in the light of these results, we propose that a modification in the -1 subsite enables the accommodation of a xylosyl side chain at this position. The stereochemical compatibility of the -1 subsite with a xylosyl moiety was also reported for other structurally nonrelated XEGs belonging to the GH74 family, indicating it to be an essential attribute for this mode of action.

-	~~The entry 4w8b is ON HOLD until Paper Publication~~	+	Structural Basis for Xyloglucan Specificity and alpha-d-Xylp(1 --> 6)-d-Glcp Recognition at the -1 Subsite within the GH5 Family.,Dos Santos CR, Cordeiro RL, Wong DW, Murakami MT Biochemistry. 2015 Mar 6. PMID:25714929<ref>PMID:25714929</ref>

-	~~Authors: Santos~~, ~~C.R., Cordeiro, R.L., Wong, D.W~~.S.~~, Murakami, M.T~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description:~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: ~~Santos~~, ~~C.R~~]]	+	__TOC__
-	[[Category: ~~Murakami~~, ~~M.T~~]]	+	</StructureSection>
-	[[Category: Wong, D.W.S]]	+	[[Category: Cordeiro, R L]]
-	[[Category: ~~Cordeiro, R.L~~]]	+	[[Category: Murakami, M T]]
		+	[[Category: Santos, C R]]
		+	[[Category: Wong, D W.S]]
		+	[[Category: Cell wall degrading enzyme]]
		+	[[Category: Gh5 family]]
		+	[[Category: Glycoside hydrolase]]

OCA at 19:12, 24 December 2014

OCA — Wed, 24 Dec 2014 19:12:08 GMT

←Older revision		Revision as of 19:12, 24 December 2014
Line 5:		Line 5:
	Authors: Santos, C.R., Cordeiro, R.L., Wong, D.W.S., Murakami, M.T.		Authors: Santos, C.R., Cordeiro, R.L., Wong, D.W.S., Murakami, M.T.

-	Description:	+	Description:
		+	[[Category: Unreleased Structures]]
		+	[[Category: Santos, C.R]]
		+	[[Category: Murakami, M.T]]
		+	[[Category: Wong, D.W.S]]
		+	[[Category: Cordeiro, R.L]]

OCA at 08:51, 10 September 2014

OCA — Wed, 10 Sep 2014 08:51:43 GMT

←Older revision		Revision as of 08:51, 10 September 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4w8b is ON HOLD	+	The entry 4w8b is ON HOLD until Paper Publication

	Authors: Santos, C.R., Cordeiro, R.L., Wong, D.W.S., Murakami, M.T.		Authors: Santos, C.R., Cordeiro, R.L., Wong, D.W.S., Murakami, M.T.

	Description:		Description:

OCA: Protected "4w8b" [edit=sysop:move=sysop]

OCA — Thu, 04 Sep 2014 04:47:56 GMT

Protected "4w8b" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:47, 4 September 2014

OCA: New page: '''Unreleased structure''' The entry 4w8b is ON HOLD Authors: Santos, C.R., Cordeiro, R.L., Wong, D.W.S., Murakami, M.T. Description:

OCA — Thu, 04 Sep 2014 04:47:55 GMT

New page: '''Unreleased structure''' The entry 4w8b is ON HOLD Authors: Santos, C.R., Cordeiro, R.L., Wong, D.W.S., Murakami, M.T. Description:

New page

'''Unreleased structure'''

The entry 4w8b is ON HOLD

Authors: Santos, C.R., Cordeiro, R.L., Wong, D.W.S., Murakami, M.T.

Description: