4w8y - Revision history

OCA at 11:27, 9 May 2024

OCA — Thu, 09 May 2024 11:27:15 GMT

←Older revision		Revision as of 11:27, 9 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4w8y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W8Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4W8Y FirstGlance]. <br>		<table><tr><td colspan='2'>[[4w8y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W8Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4W8Y FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4w8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8y OCA], [https://pdbe.org/4w8y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4w8y RCSB], [https://www.ebi.ac.uk/pdbsum/4w8y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4w8y ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4w8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8y OCA], [https://pdbe.org/4w8y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4w8y RCSB], [https://www.ebi.ac.uk/pdbsum/4w8y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4w8y ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:57, 7 April 2023

OCA — Fri, 07 Apr 2023 06:57:37 GMT

←Older revision		Revision as of 06:57, 7 April 2023
Line 3:		Line 3:
	<StructureSection load='4w8y' size='340' side='right'caption='[[4w8y]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4w8y' size='340' side='right'caption='[[4w8y]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4w8y]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pyrfu Pyrfu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W8Y OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4W8Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4w8y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W8Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4W8Y FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cmr2, PF1129 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 PYRFU])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4w8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8y OCA], [https://pdbe.org/4w8y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4w8y RCSB], [https://www.ebi.ac.uk/pdbsum/4w8y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4w8y ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4w8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8y OCA], [~~http~~://pdbe.org/4w8y PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4w8y RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4w8y PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4w8y ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CMR2_PYRFU CMR2_PYRFU]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), formerly called psiRNA (prokaryotic silencing) in this organism. Part of the Cmr ribonucleoprotein complex which has divalent cation-dependent endoribonuclease activity specific for ssRNA complementary to the crRNA, generating 5' hydroxy- and 3' phosphate or 2'-3' cyclic phosphate termini. It is not known which subunit has endoribonuclease activity. Cmr complex does not cleave ssDNA complementary to the crRNA. Cleavage of invading RNA is guided by the crRNA; substrate cleavage occurs a fixed distance (14 nt) from the 3' end of the crRNA. In vitro reconstitution shows Cmr1-2 and Cmr5 are not necessary for cleavage. Probably not the subunit that cleaves pre-crRNA, as mutation of numerous metal-binding residues have no effect on cleavage by assembled complex.<ref>PMID:19945378</ref>	+	[https://www.uniprot.org/uniprot/CMR2_PYRFU CMR2_PYRFU] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), formerly called psiRNA (prokaryotic silencing) in this organism. Part of the Cmr ribonucleoprotein complex which has divalent cation-dependent endoribonuclease activity specific for ssRNA complementary to the crRNA, generating 5' hydroxy- and 3' phosphate or 2'-3' cyclic phosphate termini. It is not known which subunit has endoribonuclease activity. Cmr complex does not cleave ssDNA complementary to the crRNA. Cleavage of invading RNA is guided by the crRNA; substrate cleavage occurs a fixed distance (14 nt) from the 3' end of the crRNA. In vitro reconstitution shows Cmr1-2 and Cmr5 are not necessary for cleavage. Probably not the subunit that cleaves pre-crRNA, as mutation of numerous metal-binding residues have no effect on cleavage by assembled complex.<ref>PMID:19945378</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 20:

	==See Also==		==See Also==
-	*[[Endonuclease\|Endonuclease]]	+	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pyrfu~~]]	+	[[Category: Pyrococcus furiosus DSM 3638]]
-	[[Category: Baumgaertner, M]]	+	[[Category: Baumgaertner M]]
-	[[Category: Benda, C]]	+	[[Category: Benda C]]
-	[[Category: Conti, E]]	+	[[Category: Conti E]]
-	[[Category: Ebert, J]]	+	[[Category: Ebert J]]
-	~~[[Category: Hd nuclease site]]~~	+
-	~~[[Category: Mn bound form]]~~	+
-	~~[[Category: Rna binding protein~~]]	+

OCA at 09:08, 23 May 2019

OCA — Thu, 23 May 2019 09:08:33 GMT

←Older revision		Revision as of 09:08, 23 May 2019
Line 1:		Line 1:

	==Structure of full length Cmr2 from Pyrococcus furiosus (Manganese bound form)==		==Structure of full length Cmr2 from Pyrococcus furiosus (Manganese bound form)==
-	<StructureSection load='4w8y' size='340' side='right' caption='[[4w8y]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='4w8y' size='340' side='right'caption='[[4w8y]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4w8y]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W8Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W8Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4w8y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrfu Pyrfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W8Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W8Y FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cmr2, PF1129 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 PYRFU])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8y OCA], [http://pdbe.org/4w8y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4w8y RCSB], [http://www.ebi.ac.uk/pdbsum/4w8y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4w8y ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8y OCA], [http://pdbe.org/4w8y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4w8y RCSB], [http://www.ebi.ac.uk/pdbsum/4w8y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4w8y ProSAT]</span></td></tr>
	</table>		</table>
Line 25:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pyrfu]]
	[[Category: Baumgaertner, M]]		[[Category: Baumgaertner, M]]
	[[Category: Benda, C]]		[[Category: Benda, C]]

OCA at 10:49, 4 August 2016

OCA — Thu, 04 Aug 2016 10:49:25 GMT

←Older revision		Revision as of 10:49, 4 August 2016
Line 1:		Line 1:
		+
	==Structure of full length Cmr2 from Pyrococcus furiosus (Manganese bound form)==		==Structure of full length Cmr2 from Pyrococcus furiosus (Manganese bound form)==
	<StructureSection load='4w8y' size='340' side='right' caption='[[4w8y]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4w8y' size='340' side='right' caption='[[4w8y]], [[Resolution\|resolution]] 3.00Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4w8y]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W8Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W8Y FirstGlance]. <br>		<table><tr><td colspan='2'>[[4w8y]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W8Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W8Y FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4w8y RCSB], [http://www.ebi.ac.uk/pdbsum/4w8y PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8y OCA], [http://pdbe.org/4w8y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4w8y RCSB], [http://www.ebi.ac.uk/pdbsum/4w8y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4w8y ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4w8y" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 13:29, 24 December 2014

OCA — Wed, 24 Dec 2014 13:29:07 GMT

←Older revision		Revision as of 13:29, 24 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4w8y RCSB], [http://www.ebi.ac.uk/pdbsum/4w8y PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4w8y RCSB], [http://www.ebi.ac.uk/pdbsum/4w8y PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CMR2_PYRFU CMR2_PYRFU]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), formerly called psiRNA (prokaryotic silencing) in this organism. Part of the Cmr ribonucleoprotein complex which has divalent cation-dependent endoribonuclease activity specific for ssRNA complementary to the crRNA, generating 5' hydroxy- and 3' phosphate or 2'-3' cyclic phosphate termini. It is not known which subunit has endoribonuclease activity. Cmr complex does not cleave ssDNA complementary to the crRNA. Cleavage of invading RNA is guided by the crRNA; substrate cleavage occurs a fixed distance (14 nt) from the 3' end of the crRNA. In vitro reconstitution shows Cmr1-2 and Cmr5 are not necessary for cleavage. Probably not the subunit that cleaves pre-crRNA, as mutation of numerous metal-binding residues have no effect on cleavage by assembled complex.<ref>PMID:19945378</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[Endonuclease\|Endonuclease]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:24, 10 December 2014

OCA — Wed, 10 Dec 2014 12:24:18 GMT

←Older revision		Revision as of 12:24, 10 December 2014
Line 18:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Baumgaertner, M.]]	+	[[Category: Baumgaertner, M]]
-	[[Category: Benda, C.]]	+	[[Category: Benda, C]]
-	[[Category: Conti, E.]]	+	[[Category: Conti, E]]
-	[[Category: Ebert, J.]]	+	[[Category: Ebert, J]]
	[[Category: Hd nuclease site]]		[[Category: Hd nuclease site]]
	[[Category: Mn bound form]]		[[Category: Mn bound form]]
	[[Category: Rna binding protein]]		[[Category: Rna binding protein]]

OCA at 10:44, 20 October 2014

OCA — Mon, 20 Oct 2014 10:44:49 GMT

←Older revision		Revision as of 10:44, 20 October 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Structure of full length Cmr2 from Pyrococcus furiosus (Manganese bound form)==
		+	<StructureSection load='4w8y' size='340' side='right' caption='[[4w8y]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4w8y]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W8Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W8Y FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w8y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4w8y RCSB], [http://www.ebi.ac.uk/pdbsum/4w8y PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Cmr complex is an RNA-guided endonuclease that cleaves foreign RNA targets as part of the CRISPR prokaryotic defense system. We investigated the molecular architecture of the P. furiosus Cmr complex using an integrative structural biology approach. We determined crystal structures of P. furiosus Cmr1, Cmr2, Cmr4, and Cmr6 and combined them with known structural information to interpret the cryo-EM map of the complex. To support structure determination, we obtained residue-specific interaction data using protein crosslinking and mass spectrometry. The resulting pseudoatomic model reveals how the superhelical backbone of the complex is defined by the polymerizing principles of Cmr4 and Cmr5 and how it is capped at the extremities by proteins of similar folds. The inner surface of the superhelix exposes conserved residues of Cmr4 that we show are required for target-cleavage activity. The structural and biochemical data thus identify Cmr4 as the conserved endoribonuclease of the Cmr complex.

-	~~The entry 4w8y is ON HOLD until Paper Publication~~	+	Structural Model of a CRISPR RNA-Silencing Complex Reveals the RNA-Target Cleavage Activity in Cmr4.,Benda C, Ebert J, Scheltema RA, Schiller HB, Baumgartner M, Bonneau F, Mann M, Conti E Mol Cell. 2014 Oct 2;56(1):43-54. doi: 10.1016/j.molcel.2014.09.002. PMID:25280103<ref>PMID:25280103</ref>

-	~~Authors: Benda~~, C.~~, Ebert, J~~., Baumgaertner, M., Conti, E.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Structure of full length Cmr2 from Pyrococcus furiosus (Manganese~~ bound form)	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Baumgaertner, M.]]
		+	[[Category: Benda, C.]]
		+	[[Category: Conti, E.]]
		+	[[Category: Ebert, J.]]
		+	[[Category: Hd nuclease site]]
		+	[[Category: Mn bound form]]
		+	[[Category: Rna binding protein]]

OCA at 09:04, 24 September 2014

OCA — Wed, 24 Sep 2014 09:04:14 GMT

←Older revision		Revision as of 09:04, 24 September 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4w8y is ON HOLD	+	The entry 4w8y is ON HOLD until Paper Publication

	Authors: Benda, C., Ebert, J., Baumgaertner, M., Conti, E.		Authors: Benda, C., Ebert, J., Baumgaertner, M., Conti, E.

	Description: Structure of full length Cmr2 from Pyrococcus furiosus (Manganese bound form)		Description: Structure of full length Cmr2 from Pyrococcus furiosus (Manganese bound form)

OCA: Protected "4w8y" [edit=sysop:move=sysop]

OCA — Thu, 04 Sep 2014 04:49:14 GMT

Protected "4w8y" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:49, 4 September 2014

OCA: New page: '''Unreleased structure''' The entry 4w8y is ON HOLD Authors: Benda, C., Ebert, J., Baumgaertner, M., Conti, E. Description: Structure of full length Cmr2 from Pyrococcus furiosus (Man...

OCA — Thu, 04 Sep 2014 04:49:12 GMT

New page: '''Unreleased structure''' The entry 4w8y is ON HOLD Authors: Benda, C., Ebert, J., Baumgaertner, M., Conti, E. Description: Structure of full length Cmr2 from Pyrococcus furiosus (Man...

New page

'''Unreleased structure'''

The entry 4w8y is ON HOLD

Authors: Benda, C., Ebert, J., Baumgaertner, M., Conti, E.

Description: Structure of full length Cmr2 from Pyrococcus furiosus (Manganese bound form)