4waa - Revision history

OCA at 10:41, 10 January 2024

OCA — Wed, 10 Jan 2024 10:41:37 GMT

←Older revision		Revision as of 10:41, 10 January 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4waa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WAA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WAA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4waa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WAA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WAA FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4waa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4waa OCA], [https://pdbe.org/4waa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4waa RCSB], [https://www.ebi.ac.uk/pdbsum/4waa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4waa ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4waa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4waa OCA], [https://pdbe.org/4waa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4waa RCSB], [https://www.ebi.ac.uk/pdbsum/4waa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4waa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 06:59, 7 April 2023

OCA — Fri, 07 Apr 2023 06:59:14 GMT

←Older revision		Revision as of 06:59, 7 April 2023
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	==Crystal structure of Nix LIR-fused human LC3B_2-119==		==Crystal structure of Nix LIR-fused human LC3B_2-119==
-	<StructureSection load='4waa' size='340' side='right' caption='[[4waa]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='4waa' size='340' side='right'caption='[[4waa]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4waa]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WAA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4WAA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4waa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WAA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WAA FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MAP1LC3B, MAP1ALC3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4waa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4waa OCA], [https://pdbe.org/4waa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4waa RCSB], [https://www.ebi.ac.uk/pdbsum/4waa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4waa ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4waa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4waa OCA], [~~http~~://pdbe.org/4waa PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4waa RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4waa PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4waa ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN]] Involved in formation of autophagosomal vacuoles (autophagosomes).	+	[https://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN] Involved in formation of autophagosomal vacuoles (autophagosomes).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 19:

	==See Also==		==See Also==
-	*[[Microtubule-associated protein\|Microtubule-associated protein]]	+	*[[Microtubule-associated protein 3D structures\|Microtubule-associated protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: Dobson~~, R C.J~~]]	+	[[Category: Large Structures]]
-	[[Category: Novak, I]]	+	[[Category: Dobson RCJ]]
-	[[Category: Ravichandran~~, A C~~]]	+	[[Category: Novak I]]
-	[[Category: Suzuki, H]]	+	[[Category: Ravichandran AC]]
-	[[Category: Wakatsuki, S]]	+	[[Category: Suzuki H]]
-	~~[[Category: Autophagy]]~~	+	[[Category: Wakatsuki S]]
-	~~[[Category: Degradation]]~~	+
-	~~[[Category: Lc3]]~~	+
-	~~[[Category: Lir]]~~	+
-	~~[[Category: Nix]]~~	+
-	~~[[Category: Recycling]]~~	+
-	~~[[Category: Selective autophagy]]~~	+
-	~~[[Category: Structural protein~~]]	+

OCA at 06:11, 20 June 2018

OCA — Wed, 20 Jun 2018 06:11:58 GMT

←Older revision		Revision as of 06:11, 20 June 2018
Line 1:		Line 1:
		+
	==Crystal structure of Nix LIR-fused human LC3B_2-119==		==Crystal structure of Nix LIR-fused human LC3B_2-119==
	<StructureSection load='4waa' size='340' side='right' caption='[[4waa]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='4waa' size='340' side='right' caption='[[4waa]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4waa]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WAA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4waa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WAA FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4waa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4waa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4waa RCSB], [http://www.ebi.ac.uk/pdbsum/4waa PDBsum]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MAP1LC3B, MAP1ALC3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4waa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4waa OCA], [http://pdbe.org/4waa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4waa RCSB], [http://www.ebi.ac.uk/pdbsum/4waa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4waa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN]] Involved in formation of autophagosomal vacuoles (autophagosomes).		[[http://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN]] Involved in formation of autophagosomal vacuoles (autophagosomes).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The mitophagy receptor Nix interacts with LC3/GABARAP proteins, targeting mitochondria into autophagosomes for degradation. Here we present evidence for phosphorylation-driven regulation of the Nix:LC3B interaction. Isothermal titration calorimetry and NMR indicate a ~100 fold enhanced affinity of the serine 34/35-phosphorylated Nix LC3-interacting region (LIR) to LC3B and formation of a very rigid complex compared to the non-phosphorylated sequence. Moreover, the crystal structure of LC3B in complex with the Nix LIR peptide containing glutamic acids as phosphomimetic residues and NMR experiments revealed that LIR phosphorylation stabilizes the Nix:LC3B complex via formation of two additional hydrogen bonds between phosphorylated serines of Nix LIR and Arg11, Lys49 and Lys51 in LC3B. Substitution of Lys51 to Ala in LC3B abrogates binding of a phosphomimetic Nix mutant. Functionally, serine 34/35 phosphorylation enhances autophagosome recruitment to mitochondria in HeLa cells. Together, this study provides cellular, biochemical and biophysical evidence that phosphorylation of the LIR domain of Nix enhances mitophagy receptor engagement.
		+
		+	Phosphorylation of the mitochondrial autophagy receptor Nix enhances its interaction with LC3 proteins.,Rogov VV, Suzuki H, Marinkovic M, Lang V, Kato R, Kawasaki M, Buljubasic M, Sprung M, Rogova N, Wakatsuki S, Hamacher-Brady A, Dotsch V, Dikic I, Brady NR, Novak I Sci Rep. 2017 Apr 25;7(1):1131. doi: 10.1038/s41598-017-01258-6. PMID:28442745<ref>PMID:28442745</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4waa" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Microtubule-associated protein\|Microtubule-associated protein]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Dobson, R C.J]]		[[Category: Dobson, R C.J]]
	[[Category: Novak, I]]		[[Category: Novak, I]]

OCA at 11:19, 9 September 2015

OCA — Wed, 09 Sep 2015 11:19:11 GMT

←Older revision		Revision as of 11:19, 9 September 2015
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-	'''~~Unreleased structure~~'''	+	==Crystal structure of Nix LIR-fused human LC3B_2-119==
-		+	<StructureSection load='4waa' size='340' side='right' caption='[[4waa]], [[Resolution\|resolution]] 2.35Å' scene=''>
-	~~The entry~~ 4waa is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4waa]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WAA FirstGlance]. <br>
-	~~Authors~~: ~~Suzuki, H~~.~~, Ravichandran, A~~.C.~~, Dobson, R~~.C.J.~~, Novak, I~~.~~, Wakatsuki, S~~.	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4waa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4waa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4waa RCSB], [http://www.ebi.ac.uk/pdbsum/4waa PDBsum]</span></td></tr>
-		+	</table>
-	~~Description~~: ~~Crystal structure of Nix LIR-fused human LC3B_2-119~~	+	== Function ==
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	[[http://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN]] Involved in formation of autophagosomal vacuoles (autophagosomes).
-	[~~[Category~~: ~~Ravichandran~~, A.C]]	+	__TOC__
-	[[~~Category~~: ~~Suzuki, H~~]]	+	</StructureSection>
-	[[Category: Dobson, R.C.J]]	+	[[Category: Dobson, R C.J]]
	[[Category: Novak, I]]		[[Category: Novak, I]]
		+	[[Category: Ravichandran, A C]]
		+	[[Category: Suzuki, H]]
	[[Category: Wakatsuki, S]]		[[Category: Wakatsuki, S]]
		+	[[Category: Autophagy]]
		+	[[Category: Degradation]]
		+	[[Category: Lc3]]
		+	[[Category: Lir]]
		+	[[Category: Nix]]
		+	[[Category: Recycling]]
		+	[[Category: Selective autophagy]]
		+	[[Category: Structural protein]]

OCA at 19:15, 24 December 2014

OCA — Wed, 24 Dec 2014 19:15:15 GMT

←Older revision		Revision as of 19:15, 24 December 2014
Line 6:		Line 6:

	Description: Crystal structure of Nix LIR-fused human LC3B_2-119		Description: Crystal structure of Nix LIR-fused human LC3B_2-119
		+	[[Category: Unreleased Structures]]
		+	[[Category: Ravichandran, A.C]]
		+	[[Category: Suzuki, H]]
		+	[[Category: Dobson, R.C.J]]
		+	[[Category: Novak, I]]
		+	[[Category: Wakatsuki, S]]

OCA at 08:53, 10 September 2014

OCA — Wed, 10 Sep 2014 08:53:08 GMT

←Older revision		Revision as of 08:53, 10 September 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4waa is ON HOLD	+	The entry 4waa is ON HOLD until Paper Publication

-	Authors: Suzuki, H., Wakatsuki, S.	+	Authors: Suzuki, H., Ravichandran, A.C., Dobson, R.C.J., Novak, I., Wakatsuki, S.

-	Description: Nix-LIR-~~LC3B~~	+	Description: Crystal structure of Nix LIR-fused human LC3B_2-119

OCA: Protected "4waa" [edit=sysop:move=sysop]

OCA — Thu, 04 Sep 2014 04:52:09 GMT

Protected "4waa" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:52, 4 September 2014

OCA: New page: '''Unreleased structure''' The entry 4waa is ON HOLD Authors: Suzuki, H., Wakatsuki, S. Description: Nix-LIR-LC3B

OCA — Thu, 04 Sep 2014 04:52:07 GMT

New page: '''Unreleased structure''' The entry 4waa is ON HOLD Authors: Suzuki, H., Wakatsuki, S. Description: Nix-LIR-LC3B

New page

'''Unreleased structure'''

The entry 4waa is ON HOLD

Authors: Suzuki, H., Wakatsuki, S.

Description: Nix-LIR-LC3B