4wal - Revision history

OCA at 00:52, 28 December 2023

OCA — Thu, 28 Dec 2023 00:52:16 GMT

←Older revision		Revision as of 00:52, 28 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4wal]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WAL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WAL FirstGlance]. <br>		<table><tr><td colspan='2'>[[4wal]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WAL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WAL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wal OCA], [https://pdbe.org/4wal PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wal RCSB], [https://www.ebi.ac.uk/pdbsum/4wal PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wal ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wal OCA], [https://pdbe.org/4wal PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wal RCSB], [https://www.ebi.ac.uk/pdbsum/4wal PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wal ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:59, 7 April 2023

OCA — Fri, 07 Apr 2023 06:59:44 GMT

←Older revision		Revision as of 06:59, 7 April 2023
Line 3:		Line 3:
	<StructureSection load='4wal' size='340' side='right'caption='[[4wal]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4wal' size='340' side='right'caption='[[4wal]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4wal]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WAL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4WAL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4wal]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WAL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WAL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=~~SO4~~:~~SULFATE+ION~~'>~~SO4~~</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene>~~</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wal OCA], [https://pdbe.org/4wal PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wal RCSB], [https://www.ebi.ac.uk/pdbsum/4wal PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wal ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wan\|4wan]]</td></tr>~~	+
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MSL5, BBP, SF1, YLR116W, L2949 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4wal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wal OCA], [~~http~~://pdbe.org/4wal PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4wal RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4wal PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4wal ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/BBP_YEAST BBP_YEAST]] Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. 2 commitment complexes, CC1 and CC2, have been defined. CC1 is a basal complex dependent only on the 5'-splice site. CC2 is a complex of lower mobility and is dependent on a branchpoint as well as a 5'-splice site region. This protein is involved in CC2 formation where it binds to the snRNP U1-associated protein PRP40, bridging the U1 snRNP-associated 5'-splice site and the MSL5-associated branch point 3' intron splice site. Involved in nuclear retention of pre-mRNA.<ref>PMID:9150140</ref> <ref>PMID:10376880</ref> <ref>PMID:10775271</ref>	+	[https://www.uniprot.org/uniprot/BBP_YEAST BBP_YEAST] Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. 2 commitment complexes, CC1 and CC2, have been defined. CC1 is a basal complex dependent only on the 5'-splice site. CC2 is a complex of lower mobility and is dependent on a branchpoint as well as a 5'-splice site region. This protein is involved in CC2 formation where it binds to the snRNP U1-associated protein PRP40, bridging the U1 snRNP-associated 5'-splice site and the MSL5-associated branch point 3' intron splice site. Involved in nuclear retention of pre-mRNA.<ref>PMID:9150140</ref> <ref>PMID:10376880</ref> <ref>PMID:10775271</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Baker's yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Chico, L~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: ~~Jacewicz, A~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Schwer, B~~]]	+	[[Category: Chico L]]
-	[[Category: ~~Shuman, S~~]]	+	[[Category: Jacewicz A]]
-	[[Category: ~~Smith, P~~]]	+	[[Category: Schwer B]]
-	[[Category: ~~Bbp~~]]	+	[[Category: Shuman S]]
-	[[Category: ~~Msl5]]~~	+	[[Category: Smith P]]
-	~~[[Category: Protein binding-rna complex]]~~	+
-	~~[[Category: Rna binding]]~~	+
-	~~[[Category: Yeast pre-mrna splicing~~]]	+

OCA at 10:28, 25 December 2019

OCA — Wed, 25 Dec 2019 10:28:18 GMT

←Older revision		Revision as of 10:28, 25 December 2019
Line 1:		Line 1:

	==Crystal structure of selenomethionine Msl5 protein in complex with RNA at 2.2 A==		==Crystal structure of selenomethionine Msl5 protein in complex with RNA at 2.2 A==
-	<StructureSection load='4wal' size='340' side='right' caption='[[4wal]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='4wal' size='340' side='right'caption='[[4wal]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4wal]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WAL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WAL FirstGlance]. <br>		<table><tr><td colspan='2'>[[4wal]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WAL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WAL FirstGlance]. <br>
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Baker's yeast]]		[[Category: Baker's yeast]]
		+	[[Category: Large Structures]]
	[[Category: Chico, L]]		[[Category: Chico, L]]
	[[Category: Jacewicz, A]]		[[Category: Jacewicz, A]]

OCA at 15:09, 15 November 2017

OCA — Wed, 15 Nov 2017 15:09:26 GMT

←Older revision		Revision as of 15:09, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='4wal' size='340' side='right' caption='[[4wal]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4wal' size='340' side='right' caption='[[4wal]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4wal]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WAL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WAL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4wal]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WAL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WAL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wan\|4wan]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wan\|4wan]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MSL5, BBP, SF1, YLR116W, L2949 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wal OCA], [http://pdbe.org/4wal PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wal RCSB], [http://www.ebi.ac.uk/pdbsum/4wal PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wal ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wal OCA], [http://pdbe.org/4wal PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wal RCSB], [http://www.ebi.ac.uk/pdbsum/4wal PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wal ProSAT]</span></td></tr>
	</table>		</table>
Line 24:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Baker's yeast]]
	[[Category: Chico, L]]		[[Category: Chico, L]]
	[[Category: Jacewicz, A]]		[[Category: Jacewicz, A]]

OCA at 00:20, 26 January 2017

OCA — Thu, 26 Jan 2017 00:20:33 GMT

←Older revision		Revision as of 00:20, 26 January 2017
Line 1:		Line 1:
		+
	==Crystal structure of selenomethionine Msl5 protein in complex with RNA at 2.2 A==		==Crystal structure of selenomethionine Msl5 protein in complex with RNA at 2.2 A==
	<StructureSection load='4wal' size='340' side='right' caption='[[4wal]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4wal' size='340' side='right' caption='[[4wal]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wan\|4wan]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wan\|4wan]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wal OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wal RCSB], [http://www.ebi.ac.uk/pdbsum/4wal PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wal OCA], [http://pdbe.org/4wal PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wal RCSB], [http://www.ebi.ac.uk/pdbsum/4wal PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wal ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4wal" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:12, 7 February 2015

OCA — Sat, 07 Feb 2015 10:12:07 GMT

←Older revision		Revision as of 10:12, 7 February 2015
Line 10:		Line 10:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/BBP_YEAST BBP_YEAST]] Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. 2 commitment complexes, CC1 and CC2, have been defined. CC1 is a basal complex dependent only on the 5'-splice site. CC2 is a complex of lower mobility and is dependent on a branchpoint as well as a 5'-splice site region. This protein is involved in CC2 formation where it binds to the snRNP U1-associated protein PRP40, bridging the U1 snRNP-associated 5'-splice site and the MSL5-associated branch point 3' intron splice site. Involved in nuclear retention of pre-mRNA.<ref>PMID:9150140</ref> <ref>PMID:10376880</ref> <ref>PMID:10775271</ref>		[[http://www.uniprot.org/uniprot/BBP_YEAST BBP_YEAST]] Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. 2 commitment complexes, CC1 and CC2, have been defined. CC1 is a basal complex dependent only on the 5'-splice site. CC2 is a complex of lower mobility and is dependent on a branchpoint as well as a 5'-splice site region. This protein is involved in CC2 formation where it binds to the snRNP U1-associated protein PRP40, bridging the U1 snRNP-associated 5'-splice site and the MSL5-associated branch point 3' intron splice site. Involved in nuclear retention of pre-mRNA.<ref>PMID:9150140</ref> <ref>PMID:10376880</ref> <ref>PMID:10775271</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Saccharomyces cerevisiae Msl5 orchestrates spliceosome assembly by binding the intron branchpoint sequence 5'-UACUAAC and, with its heterodimer partner protein Mud2, establishing cross intron-bridging interactions with the U1 snRNP at the 5' splice site. Here we define the central Msl5 KH-QUA2 domain as sufficient for branchpoint RNA recognition. The 1.8 A crystal structure of Msl5-(KH-QUA2) bound to the branchpoint highlights an extensive network of direct and water-mediated protein-RNA and intra-RNA atomic contacts at the interface that illuminate how Msl5 recognizes each nucleobase of the UACUAAC element. The Msl5 structure rationalizes a large body of mutational data and inspires new functional studies herein, which reveal how perturbations of the Msl5.RNA interface impede the splicing of specific yeast pre-mRNAs. We also identify interfacial mutations in Msl5 that bypass the essentiality of Sub2, a DExD-box ATPase implicated in displacing Msl5 from the branchpoint in exchange for the U2 snRNP. These studies establish an atomic resolution framework for understanding splice site selection and early spliceosome dynamics.
		+
		+	Structural basis for recognition of intron branchpoint RNA by yeast Msl5 and selective effects of interfacial mutations on splicing of yeast pre-mRNAs.,Jacewicz A, Chico L, Smith P, Schwer B, Shuman S RNA. 2015 Jan 13. PMID:25587180<ref>PMID:25587180</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:06, 25 December 2014

OCA — Thu, 25 Dec 2014 12:06:29 GMT

←Older revision		Revision as of 12:06, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wal OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wal RCSB], [http://www.ebi.ac.uk/pdbsum/4wal PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wal OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wal RCSB], [http://www.ebi.ac.uk/pdbsum/4wal PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BBP_YEAST BBP_YEAST]] Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. 2 commitment complexes, CC1 and CC2, have been defined. CC1 is a basal complex dependent only on the 5'-splice site. CC2 is a complex of lower mobility and is dependent on a branchpoint as well as a 5'-splice site region. This protein is involved in CC2 formation where it binds to the snRNP U1-associated protein PRP40, bridging the U1 snRNP-associated 5'-splice site and the MSL5-associated branch point 3' intron splice site. Involved in nuclear retention of pre-mRNA.<ref>PMID:9150140</ref> <ref>PMID:10376880</ref> <ref>PMID:10775271</ref>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:03, 17 December 2014

OCA — Wed, 17 Dec 2014 13:03:56 GMT

←Older revision		Revision as of 13:03, 17 December 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of selenomethionine Msl5 protein in complex with RNA at 2.2 A==
-		+	<StructureSection load='4wal' size='340' side='right' caption='[[4wal]], [[Resolution\|resolution]] 2.20Å' scene=''>
-	~~The entry~~ 4wal is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4wal]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WAL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WAL FirstGlance]. <br>
-	~~Authors~~: ~~Jacewicz, A~~., ~~Smith~~, P., Chico, L., Schwer, B., Shuman, S.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~Description~~: ~~Crystal structure of selenomethionine~~ Msl5 ~~protein in~~ complex ~~with RNA at 2.2 A~~	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wan\|4wan]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wal OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wal RCSB], [http://www.ebi.ac.uk/pdbsum/4wal PDBsum]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chico, L]]
		+	[[Category: Jacewicz, A]]
		+	[[Category: Schwer, B]]
		+	[[Category: Shuman, S]]
		+	[[Category: Smith, P]]
		+	[[Category: Bbp]]
		+	[[Category: Msl5]]
		+	[[Category: Protein binding-rna complex]]
		+	[[Category: Rna binding]]
		+	[[Category: Yeast pre-mrna splicing]]

OCA at 09:05, 24 September 2014

OCA — Wed, 24 Sep 2014 09:05:38 GMT

←Older revision		Revision as of 09:05, 24 September 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4wal is ON HOLD	+	The entry 4wal is ON HOLD until Paper Publication

	Authors: Jacewicz, A., Smith, P., Chico, L., Schwer, B., Shuman, S.		Authors: Jacewicz, A., Smith, P., Chico, L., Schwer, B., Shuman, S.

	Description: Crystal structure of selenomethionine Msl5 protein in complex with RNA at 2.2 A		Description: Crystal structure of selenomethionine Msl5 protein in complex with RNA at 2.2 A

OCA: Protected "4wal" [edit=sysop:move=sysop]

OCA — Wed, 10 Sep 2014 08:53:46 GMT

Protected "4wal" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:53, 10 September 2014