4wc9 - Revision history

OCA at 10:41, 10 January 2024

OCA — Wed, 10 Jan 2024 10:41:50 GMT

←Older revision		Revision as of 10:41, 10 January 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4wc9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WC9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4wc9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WC9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wc9 OCA], [https://pdbe.org/4wc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wc9 RCSB], [https://www.ebi.ac.uk/pdbsum/4wc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wc9 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wc9 OCA], [https://pdbe.org/4wc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wc9 RCSB], [https://www.ebi.ac.uk/pdbsum/4wc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wc9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 07:01, 7 April 2023

OCA — Fri, 07 Apr 2023 07:01:33 GMT

←Older revision		Revision as of 07:01, 7 April 2023
Line 1:		Line 1:

	==Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, with mutation F235L==		==Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, with mutation F235L==
-	<StructureSection load='4wc9' size='340' side='right' caption='[[4wc9]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4wc9' size='340' side='right'caption='[[4wc9]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4wc9]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WC9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4WC9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4wc9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WC9 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cnp, Cnp1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wc9 OCA], [https://pdbe.org/4wc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wc9 RCSB], [https://www.ebi.ac.uk/pdbsum/4wc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wc9 ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2',3'-cyclic-nucleotide_3'-phosphodiesterase 2',3'-cyclic-nucleotide 3'-phosphodiesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.37 3.1.4.37] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4wc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wc9 OCA], [~~http~~://pdbe.org/4wc9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4wc9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4wc9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4wc9 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CN37_MOUSE CN37_MOUSE]] May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin.<ref>PMID:22393399</ref>	+	[https://www.uniprot.org/uniprot/CN37_MOUSE CN37_MOUSE] May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin.<ref>PMID:22393399</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 19:

	==See Also==		==See Also==
-	*[[Phosphodiesterase\|Phosphodiesterase]]	+	*[[Phosphodiesterase 3D structures\|Phosphodiesterase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~2',3'-cyclic-nucleotide 3'-phosphodiesterase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Kursula, P]]	+	[[Category: Kursula P]]
-	[[Category: Myllykoski, M]]	+	[[Category: Myllykoski M]]
-	[[Category: Raasakka, A]]	+	[[Category: Raasakka A]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Myelin]]~~	+
-	~~[[Category: Nervous system~~]]	+

OCA at 05:57, 8 March 2018

OCA — Thu, 08 Mar 2018 05:57:16 GMT

←Older revision		Revision as of 05:57, 8 March 2018
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	</div>		</div>
	<div class="pdbe-citations 4wc9" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4wc9" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Phosphodiesterase\|Phosphodiesterase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:14, 16 November 2017

OCA — Thu, 16 Nov 2017 15:14:56 GMT

←Older revision		Revision as of 15:14, 16 November 2017
Line 1:		Line 1:
		+
	==Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, with mutation F235L==		==Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, with mutation F235L==
	<StructureSection load='4wc9' size='340' side='right' caption='[[4wc9]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4wc9' size='340' side='right' caption='[[4wc9]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4wc9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WC9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WC9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4wc9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WC9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WC9 FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2',3'-cyclic-nucleotide_3'-phosphodiesterase 2',3'-cyclic-nucleotide 3'-phosphodiesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.37 3.1.4.37] </span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cnp, Cnp1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wc9 OCA], [http://pdbe.org/4wc9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wc9 RCSB], [http://www.ebi.ac.uk/pdbsum/4wc9 PDBsum]</span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2',3'-cyclic-nucleotide_3'-phosphodiesterase 2',3'-cyclic-nucleotide 3'-phosphodiesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.37 3.1.4.37] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wc9 OCA], [http://pdbe.org/4wc9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wc9 RCSB], [http://www.ebi.ac.uk/pdbsum/4wc9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wc9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: 2',3'-cyclic-nucleotide 3'-phosphodiesterase]]		[[Category: 2',3'-cyclic-nucleotide 3'-phosphodiesterase]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Kursula, P]]		[[Category: Kursula, P]]
	[[Category: Myllykoski, M]]		[[Category: Myllykoski, M]]

OCA at 21:12, 30 November 2015

OCA — Mon, 30 Nov 2015 21:12:13 GMT

←Older revision		Revision as of 21:12, 30 November 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, with mutation F235L==
		+	<StructureSection load='4wc9' size='340' side='right' caption='[[4wc9]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4wc9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WC9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WC9 FirstGlance]. <br>
		+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2',3'-cyclic-nucleotide_3'-phosphodiesterase 2',3'-cyclic-nucleotide 3'-phosphodiesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.37 3.1.4.37] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wc9 OCA], [http://pdbe.org/4wc9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wc9 RCSB], [http://www.ebi.ac.uk/pdbsum/4wc9 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CN37_MOUSE CN37_MOUSE]] May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin.<ref>PMID:22393399</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) is an enzyme highly abundant in the central nervous system myelin of terrestrial vertebrates. The catalytic domain of CNPase belongs to the 2H phosphoesterase superfamily and catalyzes the hydrolysis of nucleoside 2',3'-cyclic monophosphates to nucleoside 2'-monophosphates. The detailed reaction mechanism and the essential catalytic amino acids involved have been described earlier, but the roles of many amino acids in the vicinity of the active site have remained unknown. Here, several CNPase catalytic domain mutants were studied using enzyme kinetics assays, thermal stability experiments, and X-ray crystallography. Additionally, the crystal structure of a perdeuterated CNPase catalytic domain was refined at atomic resolution to obtain a detailed view of the active site and the catalytic mechanism. The results specify determinants of ligand binding and novel essential residues required for CNPase catalysis. For example, the aromatic side chains of Phe235 and Tyr168 are crucial for substrate binding, and Arg307 may affect active site electrostatics and regulate loop dynamics. The beta5-alpha7 loop, unique for CNPase in the 2H phosphoesterase family, appears to have various functions in the CNPase reaction mechanism, from coordinating the nucleophilic water molecule to providing a binding pocket for the product and being involved in product release.

-	~~The entry 4wc9 is ON HOLD until Paper Publication~~	+	Determinants of ligand binding and catalytic activity in the myelin enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase.,Raasakka A, Myllykoski M, Laulumaa S, Lehtimaki M, Hartlein M, Moulin M, Kursula I, Kursula P Sci Rep. 2015 Nov 13;5:16520. doi: 10.1038/srep16520. PMID:26563764<ref>PMID:26563764</ref>

-	~~Authors: Myllykoski~~, M.~~, Raasakka, A~~.~~, Kursula, P~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Catalytic domain of mouse~~ 2',3'-cyclic nucleotide 3'-phosphodiesterase~~, with mutation F235L~~	+	<div class="pdbe-citations 4wc9" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: 2',3'-cyclic-nucleotide 3'-phosphodiesterase]]
		+	[[Category: Kursula, P]]
	[[Category: Myllykoski, M]]		[[Category: Myllykoski, M]]
	[[Category: Raasakka, A]]		[[Category: Raasakka, A]]
-	[[Category: ~~Kursula, P~~]]	+	[[Category: Hydrolase]]
		+	[[Category: Myelin]]
		+	[[Category: Nervous system]]

OCA at 19:19, 24 December 2014

OCA — Wed, 24 Dec 2014 19:19:08 GMT

←Older revision		Revision as of 19:19, 24 December 2014
Line 6:		Line 6:

	Description: Catalytic domain of mouse 2',3'-cyclic nucleotide 3'-phosphodiesterase, with mutation F235L		Description: Catalytic domain of mouse 2',3'-cyclic nucleotide 3'-phosphodiesterase, with mutation F235L
		+	[[Category: Unreleased Structures]]
		+	[[Category: Myllykoski, M]]
		+	[[Category: Raasakka, A]]
		+	[[Category: Kursula, P]]

OCA at 09:07, 24 September 2014

OCA — Wed, 24 Sep 2014 09:07:52 GMT

←Older revision		Revision as of 09:07, 24 September 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4wc9 is ON HOLD	+	The entry 4wc9 is ON HOLD until Paper Publication

	Authors: Myllykoski, M., Raasakka, A., Kursula, P.		Authors: Myllykoski, M., Raasakka, A., Kursula, P.

	Description: Catalytic domain of mouse 2',3'-cyclic nucleotide 3'-phosphodiesterase, with mutation F235L		Description: Catalytic domain of mouse 2',3'-cyclic nucleotide 3'-phosphodiesterase, with mutation F235L

OCA: Protected "4wc9" [edit=sysop:move=sysop]

OCA — Wed, 10 Sep 2014 08:57:04 GMT

Protected "4wc9" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:57, 10 September 2014

OCA: New page: '''Unreleased structure''' The entry 4wc9 is ON HOLD Authors: Myllykoski, M., Raasakka, A., Kursula, P. Description: Catalytic domain of mouse 2',3'-cyclic nucleotide 3'-phosphodiester...

OCA — Wed, 10 Sep 2014 08:57:01 GMT

New page: '''Unreleased structure''' The entry 4wc9 is ON HOLD Authors: Myllykoski, M., Raasakka, A., Kursula, P. Description: Catalytic domain of mouse 2',3'-cyclic nucleotide 3'-phosphodiester...

New page

'''Unreleased structure'''

The entry 4wc9 is ON HOLD

Authors: Myllykoski, M., Raasakka, A., Kursula, P.

Description: Catalytic domain of mouse 2',3'-cyclic nucleotide 3'-phosphodiesterase, with mutation F235L