4wf2 - Revision history

OCA at 00:52, 28 December 2023

OCA — Thu, 28 Dec 2023 00:52:57 GMT

←Older revision		Revision as of 00:52, 28 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4wf2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WF2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WF2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4wf2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WF2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WF2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTX:((2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXY-TETRAHYDROFURAN-2-YL)METHYL+5-((3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL)PENTYL+HYDROGEN+PHOSPHATE'>BTX</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.31Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTX:((2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXY-TETRAHYDROFURAN-2-YL)METHYL+5-((3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL)PENTYL+HYDROGEN+PHOSPHATE'>BTX</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wf2 OCA], [https://pdbe.org/4wf2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wf2 RCSB], [https://www.ebi.ac.uk/pdbsum/4wf2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wf2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wf2 OCA], [https://pdbe.org/4wf2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wf2 RCSB], [https://www.ebi.ac.uk/pdbsum/4wf2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wf2 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:05, 7 April 2023

OCA — Fri, 07 Apr 2023 07:05:18 GMT

←Older revision		Revision as of 07:05, 7 April 2023
Line 3:		Line 3:
	<StructureSection load='4wf2' size='340' side='right'caption='[[4wf2]], [[Resolution\|resolution]] 2.31Å' scene=''>		<StructureSection load='4wf2' size='340' side='right'caption='[[4wf2]], [[Resolution\|resolution]] 2.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4wf2]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WF2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4WF2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4wf2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WF2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WF2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTX:((2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXY-TETRAHYDROFURAN-2-YL)METHYL+5-((3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL)PENTYL+HYDROGEN+PHOSPHATE'>BTX</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTX:((2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXY-TETRAHYDROFURAN-2-YL)METHYL+5-((3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL)PENTYL+HYDROGEN+PHOSPHATE'>BTX</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">birA, bioR, dhbB, b3973, JW3941 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wf2 OCA], [https://pdbe.org/4wf2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wf2 RCSB], [https://www.ebi.ac.uk/pdbsum/4wf2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wf2 ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Biotin--[acetyl-CoA-carboxylase]_ligase Biotin--[acetyl-CoA-carboxylase] ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.15 6.3.4.15] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4wf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wf2 OCA], [~~http~~://pdbe.org/4wf2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4wf2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4wf2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4wf2 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/BIRA_ECOLI BIRA_ECOLI]] Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.<ref>PMID:6129246</ref> <ref>PMID:2667763</ref> <ref>PMID:8003500</ref> <ref>PMID:12527300</ref>	+	[https://www.uniprot.org/uniprot/BIRA_ECOLI BIRA_ECOLI] Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.<ref>PMID:6129246</ref> <ref>PMID:2667763</ref> <ref>PMID:8003500</ref> <ref>PMID:12527300</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Beckett, D]]	+	[[Category: Beckett D]]
-	[[Category: Eginton, C]]	+	[[Category: Eginton C]]
-	[[Category: Wade, H]]	+	[[Category: Wade H]]
-	~~[[Category: Biotin protein ligase]]~~	+
-	~~[[Category: Biotin repressor]]~~	+
-	~~[[Category: Complex with biotinol-5'-amp]]~~	+
-	~~[[Category: G142a mutant]]~~	+
-	~~[[Category: Ligase~~]]	+

OCA at 07:20, 27 November 2019

OCA — Wed, 27 Nov 2019 07:20:27 GMT

←Older revision		Revision as of 07:20, 27 November 2019
Line 1:		Line 1:

	==Structure of E. coli BirA G142A bound to biotinol-5'-AMP==		==Structure of E. coli BirA G142A bound to biotinol-5'-AMP==
-	<StructureSection load='4wf2' size='340' side='right' caption='[[4wf2]], [[Resolution\|resolution]] 2.31Å' scene=''>	+	<StructureSection load='4wf2' size='340' side='right'caption='[[4wf2]], [[Resolution\|resolution]] 2.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4wf2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WF2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4wf2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WF2 FirstGlance]. <br>
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 4wf2" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4wf2" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Biotin Protein Ligase 3D structures\|Biotin Protein Ligase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 25:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Beckett, D]]		[[Category: Beckett, D]]
	[[Category: Eginton, C]]		[[Category: Eginton, C]]

OCA at 12:11, 6 November 2017

OCA — Mon, 06 Nov 2017 12:11:40 GMT

←Older revision		Revision as of 12:11, 6 November 2017
Line 3:		Line 3:
	<StructureSection load='4wf2' size='340' side='right' caption='[[4wf2]], [[Resolution\|resolution]] 2.31Å' scene=''>		<StructureSection load='4wf2' size='340' side='right' caption='[[4wf2]], [[Resolution\|resolution]] 2.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4wf2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WF2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4wf2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WF2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTX:((2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXY-TETRAHYDROFURAN-2-YL)METHYL+5-((3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL)PENTYL+HYDROGEN+PHOSPHATE'>BTX</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTX:((2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXY-TETRAHYDROFURAN-2-YL)METHYL+5-((3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL)PENTYL+HYDROGEN+PHOSPHATE'>BTX</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">birA, bioR, dhbB, b3973, JW3941 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Biotin--[acetyl-CoA-carboxylase]_ligase Biotin--[acetyl-CoA-carboxylase] ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.15 6.3.4.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Biotin--[acetyl-CoA-carboxylase]_ligase Biotin--[acetyl-CoA-carboxylase] ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.15 6.3.4.15] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wf2 OCA], [http://pdbe.org/4wf2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wf2 RCSB], [http://www.ebi.ac.uk/pdbsum/4wf2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wf2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wf2 OCA], [http://pdbe.org/4wf2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wf2 RCSB], [http://www.ebi.ac.uk/pdbsum/4wf2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wf2 ProSAT]</span></td></tr>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
	[[Category: Beckett, D]]		[[Category: Beckett, D]]
	[[Category: Eginton, C]]		[[Category: Eginton, C]]
Line 30:		Line 32:
	[[Category: Complex with biotinol-5'-amp]]		[[Category: Complex with biotinol-5'-amp]]
	[[Category: G142a mutant]]		[[Category: G142a mutant]]
		+	[[Category: Ligase]]

OCA at 00:16, 26 January 2017

OCA — Thu, 26 Jan 2017 00:16:12 GMT

←Older revision		Revision as of 00:16, 26 January 2017
Line 1:		Line 1:
		+
	==Structure of E. coli BirA G142A bound to biotinol-5'-AMP==		==Structure of E. coli BirA G142A bound to biotinol-5'-AMP==
	<StructureSection load='4wf2' size='340' side='right' caption='[[4wf2]], [[Resolution\|resolution]] 2.31Å' scene=''>		<StructureSection load='4wf2' size='340' side='right' caption='[[4wf2]], [[Resolution\|resolution]] 2.31Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTX:((2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXY-TETRAHYDROFURAN-2-YL)METHYL+5-((3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL)PENTYL+HYDROGEN+PHOSPHATE'>BTX</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTX:((2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXY-TETRAHYDROFURAN-2-YL)METHYL+5-((3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL)PENTYL+HYDROGEN+PHOSPHATE'>BTX</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Biotin--[acetyl-CoA-carboxylase]_ligase Biotin--[acetyl-CoA-carboxylase] ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.15 6.3.4.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Biotin--[acetyl-CoA-carboxylase]_ligase Biotin--[acetyl-CoA-carboxylase] ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.15 6.3.4.15] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wf2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wf2 RCSB], [http://www.ebi.ac.uk/pdbsum/4wf2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wf2 OCA], [http://pdbe.org/4wf2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wf2 RCSB], [http://www.ebi.ac.uk/pdbsum/4wf2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wf2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4wf2" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:38, 26 March 2015

OCA — Thu, 26 Mar 2015 07:38:02 GMT

←Older revision		Revision as of 07:38, 26 March 2015
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/BIRA_ECOLI BIRA_ECOLI]] Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.<ref>PMID:6129246</ref> <ref>PMID:2667763</ref> <ref>PMID:8003500</ref> <ref>PMID:12527300</ref>		[[http://www.uniprot.org/uniprot/BIRA_ECOLI BIRA_ECOLI]] Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.<ref>PMID:6129246</ref> <ref>PMID:2667763</ref> <ref>PMID:8003500</ref> <ref>PMID:12527300</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Intrinsic disorder provides a means of maximizing allosteric coupling in proteins. However, the mechanisms by which the disorder functions in allostery remain to be elucidated. Small ligand, bio-5'-AMP, binding and dimerization of the Escherichiacoli biotin repressor are allosterically coupled. Folding of a disordered loop in the allosteric effector binding site is required to realize the full coupling free energy of -4.0+/-0.3kcal/mol observed in the wild-type protein. Alanine substitution of a glycine residue on the dimerization surface that does not directly contribute to the dimerization interface completely abolishes this coupling. In this work, the structure of this variant, solved by X-ray crystallography, reveals a monomeric corepressor-bound protein. In the structure loops, neither of which contains the alanine substitution, on both the dimerization and effector binding surfaces that are folded in the corepressor-bound wild-type protein are disordered. The structural data combined with functional measurements indicate that allosteric coupling between ligand binding and dimerization in BirA (E. coli biotin repressor/biotin protein ligase) is achieved via reciprocal communication of disorder-to-order transitions on two distant functional surfaces.
		+
		+	Allosteric Coupling via Distant Disorder-to-Order Transitions.,Eginton C, Cressman WJ, Bachas S, Wade H, Beckett D J Mol Biol. 2015 Mar 4. pii: S0022-2836(15)00155-2. doi:, 10.1016/j.jmb.2015.02.021. PMID:25746672<ref>PMID:25746672</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:45, 25 December 2014

OCA — Thu, 25 Dec 2014 12:45:39 GMT

←Older revision		Revision as of 12:45, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wf2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wf2 RCSB], [http://www.ebi.ac.uk/pdbsum/4wf2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wf2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wf2 RCSB], [http://www.ebi.ac.uk/pdbsum/4wf2 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BIRA_ECOLI BIRA_ECOLI]] Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.<ref>PMID:6129246</ref> <ref>PMID:2667763</ref> <ref>PMID:8003500</ref> <ref>PMID:12527300</ref>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Beckett, D.]]	+	[[Category: Beckett, D]]
-	[[Category: Eginton, C.]]	+	[[Category: Eginton, C]]
-	[[Category: Wade, H.]]	+	[[Category: Wade, H]]
	[[Category: Biotin protein ligase]]		[[Category: Biotin protein ligase]]
	[[Category: Biotin repressor]]		[[Category: Biotin repressor]]
	[[Category: Complex with biotinol-5'-amp]]		[[Category: Complex with biotinol-5'-amp]]
	[[Category: G142a mutant]]		[[Category: G142a mutant]]

OCA at 12:31, 22 October 2014

OCA — Wed, 22 Oct 2014 12:31:47 GMT

←Older revision		Revision as of 12:31, 22 October 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Structure of E. coli BirA G142A bound to biotinol-5'-AMP==
-		+	<StructureSection load='4wf2' size='340' side='right' caption='[[4wf2]], [[Resolution\|resolution]] 2.31Å' scene=''>
-	~~The entry~~ 4wf2 is ~~ON HOLD~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4wf2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WF2 FirstGlance]. <br>
-	~~Authors~~: ~~Eginton~~, C., Beckett, D., Wade, H.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTX:((2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXY-TETRAHYDROFURAN-2-YL)METHYL+5-((3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL)PENTYL+HYDROGEN+PHOSPHATE'>BTX</scene></td></tr>
-		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Biotin--[acetyl-CoA-carboxylase]_ligase Biotin--[acetyl-CoA-carboxylase] ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.15 6.3.4.15] </span></td></tr>
-	~~Description~~: ~~Structure of E. coli BirA G142A bound to~~ biotinol-5'-~~AMP~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wf2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wf2 RCSB], [http://www.ebi.ac.uk/pdbsum/4wf2 PDBsum]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Beckett, D.]]
		+	[[Category: Eginton, C.]]
		+	[[Category: Wade, H.]]
		+	[[Category: Biotin protein ligase]]
		+	[[Category: Biotin repressor]]
		+	[[Category: Complex with biotinol-5'-amp]]
		+	[[Category: G142a mutant]]

OCA: Protected "4wf2" [edit=sysop:move=sysop]

OCA — Wed, 24 Sep 2014 09:13:02 GMT

Protected "4wf2" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:13, 24 September 2014

OCA: New page: '''Unreleased structure''' The entry 4wf2 is ON HOLD Authors: Eginton, C., Beckett, D., Wade, H. Description: Structure of E. coli BirA G142A bound to biotinol-5'-AMP

OCA — Wed, 24 Sep 2014 09:13:00 GMT

New page: '''Unreleased structure''' The entry 4wf2 is ON HOLD Authors: Eginton, C., Beckett, D., Wade, H. Description: Structure of E. coli BirA G142A bound to biotinol-5'-AMP

New page

'''Unreleased structure'''

The entry 4wf2 is ON HOLD

Authors: Eginton, C., Beckett, D., Wade, H.

Description: Structure of E. coli BirA G142A bound to biotinol-5'-AMP