4whn - Revision history

OCA at 11:27, 9 May 2024

OCA — Thu, 09 May 2024 11:27:45 GMT

←Older revision		Revision as of 11:27, 9 May 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4whn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinobacillus_pleuropneumoniae Actinobacillus pleuropneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WHN FirstGlance]. <br>		<table><tr><td colspan='2'>[[4whn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinobacillus_pleuropneumoniae Actinobacillus pleuropneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WHN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4whn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4whn OCA], [https://pdbe.org/4whn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4whn RCSB], [https://www.ebi.ac.uk/pdbsum/4whn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4whn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4whn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4whn OCA], [https://pdbe.org/4whn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4whn RCSB], [https://www.ebi.ac.uk/pdbsum/4whn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4whn ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:08, 7 April 2023

OCA — Fri, 07 Apr 2023 07:08:57 GMT

←Older revision		Revision as of 07:08, 7 April 2023
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	<StructureSection load='4whn' size='340' side='right'caption='[[4whn]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4whn' size='340' side='right'caption='[[4whn]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4whn]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Actpl Actpl~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4WHN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4whn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinobacillus_pleuropneumoniae Actinobacillus pleuropneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WHN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apxIC, clyIC, hlyIC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=715 ACTPL])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4whn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4whn OCA], [https://pdbe.org/4whn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4whn RCSB], [https://www.ebi.ac.uk/pdbsum/4whn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4whn ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4whn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4whn OCA], [~~http~~://pdbe.org/4whn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4whn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4whn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4whn ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/RTX1C_ACTPL RTX1C_ACTPL]] Involved in fatty acylation of the protoxin, thereby converting it to the active toxin.	+	[https://www.uniprot.org/uniprot/RTX1C_ACTPL RTX1C_ACTPL] Involved in fatty acylation of the protoxin, thereby converting it to the active toxin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Actpl~~]]	+	[[Category: Actinobacillus pleuropneumoniae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Crow, A]]	+	[[Category: Crow A]]
-	[[Category: Greene~~, N P~~]]	+	[[Category: Greene NP]]
-	[[Category: Hughes, C]]	+	[[Category: Hughes C]]
-	[[Category: Koronakis, V]]	+	[[Category: Koronakis V]]
-	~~[[Category: Acp binding]]~~	+
-	~~[[Category: Gnat]]~~	+
-	~~[[Category: Taat]]~~	+
-	~~[[Category: Toxin-activating acyltransferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 08:02, 23 May 2019

OCA — Thu, 23 May 2019 08:02:28 GMT

←Older revision		Revision as of 08:02, 23 May 2019
Line 1:		Line 1:

	==Structure of toxin-activating acyltransferase (TAAT)==		==Structure of toxin-activating acyltransferase (TAAT)==
-	<StructureSection load='4whn' size='340' side='right' caption='[[4whn]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='4whn' size='340' side='right'caption='[[4whn]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4whn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WHN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4whn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Actpl Actpl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WHN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apxIC, clyIC, hlyIC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=715 ACTPL])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4whn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4whn OCA], [http://pdbe.org/4whn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4whn RCSB], [http://www.ebi.ac.uk/pdbsum/4whn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4whn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4whn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4whn OCA], [http://pdbe.org/4whn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4whn RCSB], [http://www.ebi.ac.uk/pdbsum/4whn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4whn ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Actpl]]
		+	[[Category: Large Structures]]
	[[Category: Crow, A]]		[[Category: Crow, A]]
	[[Category: Greene, N P]]		[[Category: Greene, N P]]

OCA at 17:06, 22 March 2017

OCA — Wed, 22 Mar 2017 17:06:25 GMT

←Older revision		Revision as of 17:06, 22 March 2017
Line 1:		Line 1:
		+
	==Structure of toxin-activating acyltransferase (TAAT)==		==Structure of toxin-activating acyltransferase (TAAT)==
	<StructureSection load='4whn' size='340' side='right' caption='[[4whn]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4whn' size='340' side='right' caption='[[4whn]], [[Resolution\|resolution]] 2.15Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4whn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WHN FirstGlance]. <br>		<table><tr><td colspan='2'>[[4whn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WHN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4whn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4whn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4whn RCSB], [http://www.ebi.ac.uk/pdbsum/4whn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4whn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4whn OCA], [http://pdbe.org/4whn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4whn RCSB], [http://www.ebi.ac.uk/pdbsum/4whn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4whn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4whn" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:43, 10 June 2015

OCA — Wed, 10 Jun 2015 07:43:03 GMT

←Older revision		Revision as of 07:43, 10 June 2015
Line 8:		Line 8:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/RTX1C_ACTPL RTX1C_ACTPL]] Involved in fatty acylation of the protoxin, thereby converting it to the active toxin.		[[http://www.uniprot.org/uniprot/RTX1C_ACTPL RTX1C_ACTPL]] Involved in fatty acylation of the protoxin, thereby converting it to the active toxin.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Secreted pore-forming toxins of pathogenic Gram-negative bacteria such as Escherichia coli hemolysin (HlyA) insert into host-cell membranes to subvert signal transduction and induce apoptosis and cell lysis. Unusually, these toxins are synthesized in an inactive form that requires posttranslational activation in the bacterial cytosol. We have previously shown that the activation mechanism is an acylation event directed by a specialized acyl-transferase that uses acyl carrier protein (ACP) to covalently link fatty acids, via an amide bond, to specific internal lysine residues of the protoxin. We now reveal the 2.15-A resolution X-ray structure of the 172-aa ApxC, a toxin-activating acyl-transferase (TAAT) from pathogenic Actinobacillus pleuropneumoniae. This determination shows that bacterial TAATs are a structurally homologous family that, despite indiscernible sequence similarity, form a distinct branch of the Gcn5-like N-acetyl transferase (GNAT) superfamily of enzymes that typically use acyl-CoA to modify diverse bacterial, archaeal, and eukaryotic substrates. A combination of structural analysis, small angle X-ray scattering, mutagenesis, and cross-linking defined the solution state of TAATs, with intermonomer interactions mediated by an N-terminal alpha-helix. Superposition of ApxC with substrate-bound GNATs, and assay of toxin activation and binding of acyl-ACP and protoxin peptide substrates by mutated ApxC variants, indicates the enzyme active site to be a deep surface groove.
		+
		+	Structure of a bacterial toxin-activating acyltransferase.,Greene NP, Crow A, Hughes C, Koronakis V Proc Natl Acad Sci U S A. 2015 May 27. pii: 201503832. PMID:26016525<ref>PMID:26016525</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 12:44, 3 June 2015

OCA — Wed, 03 Jun 2015 12:44:44 GMT

←Older revision		Revision as of 12:44, 3 June 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Structure of toxin-activating acyltransferase (TAAT)==
-		+	<StructureSection load='4whn' size='340' side='right' caption='[[4whn]], [[Resolution\|resolution]] 2.15Å' scene=''>
-	~~The entry~~ 4whn is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4whn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WHN FirstGlance]. <br>
-	~~Authors~~: ~~Crow, A~~.~~, Greene, N~~.P.~~, Hughes, C~~.~~, Koronakis, V~~.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
-		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4whn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4whn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4whn RCSB], [http://www.ebi.ac.uk/pdbsum/4whn PDBsum]</span></td></tr>
-	~~Description~~: ~~Structure of toxin~~-~~activating acyltransferase (TAAT)~~	+	</table>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	== Function ==
-	[[~~Category~~: ~~Greene, N~~.P]]	+	[[http://www.uniprot.org/uniprot/RTX1C_ACTPL RTX1C_ACTPL]] Involved in fatty acylation of the protoxin, thereby converting it to the active toxin.
		+	__TOC__
		+	</StructureSection>
	[[Category: Crow, A]]		[[Category: Crow, A]]
-	[[Category: ~~Koronakis~~, V]]	+	[[Category: Greene, N P]]
	[[Category: Hughes, C]]		[[Category: Hughes, C]]
		+	[[Category: Koronakis, V]]
		+	[[Category: Acp binding]]
		+	[[Category: Gnat]]
		+	[[Category: Taat]]
		+	[[Category: Toxin-activating acyltransferase]]
		+	[[Category: Transferase]]

OCA at 19:30, 24 December 2014

OCA — Wed, 24 Dec 2014 19:30:35 GMT

←Older revision		Revision as of 19:30, 24 December 2014
Line 6:		Line 6:

	Description: Structure of toxin-activating acyltransferase (TAAT)		Description: Structure of toxin-activating acyltransferase (TAAT)
		+	[[Category: Unreleased Structures]]
		+	[[Category: Greene, N.P]]
		+	[[Category: Crow, A]]
		+	[[Category: Koronakis, V]]
		+	[[Category: Hughes, C]]

OCA at 06:55, 8 October 2014

OCA — Wed, 08 Oct 2014 06:55:37 GMT

←Older revision		Revision as of 06:55, 8 October 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4whn is ON HOLD	+	The entry 4whn is ON HOLD until Paper Publication

	Authors: Crow, A., Greene, N.P., Hughes, C., Koronakis, V.		Authors: Crow, A., Greene, N.P., Hughes, C., Koronakis, V.

	Description: Structure of toxin-activating acyltransferase (TAAT)		Description: Structure of toxin-activating acyltransferase (TAAT)

OCA: Protected "4whn" [edit=sysop:move=sysop]

OCA — Wed, 01 Oct 2014 23:33:33 GMT

Protected "4whn" [edit=sysop:move=sysop]

←Older revision

Revision as of 23:33, 1 October 2014

OCA: New page: '''Unreleased structure''' The entry 4whn is ON HOLD Authors: Crow, A., Greene, N.P., Hughes, C., Koronakis, V. Description: Structure of toxin-activating acyltransferase (TAAT)

OCA — Wed, 01 Oct 2014 23:33:32 GMT

New page: '''Unreleased structure''' The entry 4whn is ON HOLD Authors: Crow, A., Greene, N.P., Hughes, C., Koronakis, V. Description: Structure of toxin-activating acyltransferase (TAAT)

New page

'''Unreleased structure'''

The entry 4whn is ON HOLD

Authors: Crow, A., Greene, N.P., Hughes, C., Koronakis, V.

Description: Structure of toxin-activating acyltransferase (TAAT)