4why - Revision history

OCA at 07:09, 7 April 2023

OCA — Fri, 07 Apr 2023 07:09:17 GMT

←Older revision		Revision as of 07:09, 7 April 2023
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	<StructureSection load='4why' size='340' side='right'caption='[[4why]], [[Resolution\|resolution]] 2.62Å' scene=''>		<StructureSection load='4why' size='340' side='right'caption='[[4why]], [[Resolution\|resolution]] 2.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4why]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Buffalo_rat Buffalo rat~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4WHY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4why]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Hepacivirus_C Hepacivirus C] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WHY FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wht\|4wht]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4why FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4why OCA], [https://pdbe.org/4why PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4why RCSB], [https://www.ebi.ac.uk/pdbsum/4why PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4why ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IgG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>~~	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4why FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4why OCA], [~~http~~://pdbe.org/4why PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4why RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4why PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4why ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9WJJ4_9HEPC Q9WJJ4_9HEPC]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
	*[[Antibody 3D structures\|Antibody 3D structures]]		*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[3D structures of non-human antibody\|3D structures of non-human antibody]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Buffalo rat~~]]	+	[[Category: Hepacivirus C]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Krey, T~~]]	+	[[Category: Rattus norvegicus]]
-	[[Category: ~~Rey, F A~~]]	+	[[Category: Krey T]]
-	[[Category: ~~E2]]~~	+	[[Category: Rey FA]]
-	~~[[Category: Envelope glycoprotein]]~~	+
-	~~[[Category: Neutralizing epitope]]~~	+
-	~~[[Category: Receptor-binding]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 07:51, 23 May 2019

OCA — Thu, 23 May 2019 07:51:52 GMT

←Older revision		Revision as of 07:51, 23 May 2019
Line 1:		Line 1:

	==Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 bound to the broadly neutralizing antibody 3/11, P21 crystal form==		==Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 bound to the broadly neutralizing antibody 3/11, P21 crystal form==
-	<StructureSection load='4why' size='340' side='right' caption='[[4why]], [[Resolution\|resolution]] 2.62Å' scene=''>	+	<StructureSection load='4why' size='340' side='right'caption='[[4why]], [[Resolution\|resolution]] 2.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4why]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WHY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4why]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WHY FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wht\|4wht]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wht\|4wht]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IgG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4why FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4why OCA], [http://pdbe.org/4why PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4why RCSB], [http://www.ebi.ac.uk/pdbsum/4why PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4why ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4why FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4why OCA], [http://pdbe.org/4why PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4why RCSB], [http://www.ebi.ac.uk/pdbsum/4why PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4why ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 4why" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4why" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Buffalo rat]]
		+	[[Category: Large Structures]]
	[[Category: Krey, T]]		[[Category: Krey, T]]
	[[Category: Rey, F A]]		[[Category: Rey, F A]]

OCA at 20:31, 11 August 2016

OCA — Thu, 11 Aug 2016 20:31:04 GMT

←Older revision		Revision as of 20:31, 11 August 2016
Line 1:		Line 1:
		+
	==Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 bound to the broadly neutralizing antibody 3/11, P21 crystal form==		==Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 bound to the broadly neutralizing antibody 3/11, P21 crystal form==
	<StructureSection load='4why' size='340' side='right' caption='[[4why]], [[Resolution\|resolution]] 2.62Å' scene=''>		<StructureSection load='4why' size='340' side='right' caption='[[4why]], [[Resolution\|resolution]] 2.62Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4why]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WHY FirstGlance]. <br>		<table><tr><td colspan='2'>[[4why]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WHY FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wht\|4wht]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wht\|4wht]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4why FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4why OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4why RCSB], [http://www.ebi.ac.uk/pdbsum/4why PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4why FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4why OCA], [http://pdbe.org/4why PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4why RCSB], [http://www.ebi.ac.uk/pdbsum/4why PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4why ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4why" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:36, 17 December 2014

OCA — Wed, 17 Dec 2014 12:36:53 GMT

←Older revision		Revision as of 12:36, 17 December 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 bound to the broadly neutralizing antibody 3/11, P21 crystal form==
		+	<StructureSection load='4why' size='340' side='right' caption='[[4why]], [[Resolution\|resolution]] 2.62Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4why]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WHY FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wht\|4wht]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4why FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4why OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4why RCSB], [http://www.ebi.ac.uk/pdbsum/4why PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Neutralizing antibodies (nAbs) targeting glycoprotein E2 are important to control hepatitis C virus (HCV) infection. One conserved antigenic site (amino acids 412-423) is disordered in the reported E2 structure, but a synthetic peptide mimicking this site forms a beta-hairpin in complex with three independent nAbs. Our structure of the same peptide in complex with nAb 3/11 demonstrates a strikingly different extended conformation. We also show that residues 412-423 are essential for virus entry, but not for E2 folding. Together with the neutralizing capacity of the 3/11 Fab this indicates an unexpected structural flexibility within this epitope. NAbs 3/11 and AP33 (recognizing the extended and beta-hairpin conformation, respectively) display similar neutralizing activity despite converse binding kinetics. Our results suggest that HCV utilizes conformational flexibility as immune evasion strategy contributing to the limited immunogenicity of this epitope in patients, similar to the conformational flexibility described for other enveloped and non-enveloped viruses. IMPORTANCE: Approximately 180 million people worldwide are infected with Hepatitis C virus (HCV) and neutralizing antibodies play an important role in controlling replication of this major human pathogen. We show here that one of the most conserved antigenic sites within the major glycoprotein E2 (amino acids 412-423) - that is disordered in the recently reported crystal structure of an E2 core fragment - can adopt different conformations in the context of the infectious virus particle. Recombinant Fab fragments recognizing different conformations of this antigenic site have similar neutralization activity in spite of converse kinetic binding parameters. Of note, an antibody response targeting this antigenic region is less frequent than to other more immunogenic regions in E2. Our results suggest that the observed conformational flexibility in this conserved antigenic region contributes to the evasion of the humoral host immune response, facilitating chronicity and viral spread of HCV within an infected individual.

-	~~The entry 4why is ON HOLD~~	+	Structural Flexibility of a Conserved Broadly Neutralizing Epitope in Hepatitis C Virus Glycoprotein E2.,Meola A, Tarr AW, England P, Meredith LW, McClure CP, Foung SK, McKeating JA, Ball JK, Rey FA, Krey T J Virol. 2014 Dec 3. pii: JVI.02190-14. PMID:25473061<ref>PMID:25473061</ref>

-	~~Authors~~: Krey, T., Rey, F.A.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Structure of the Hepatitis C virus envelope~~ glycoprotein ~~E2 antigenic region 412~~-~~423 bound to the broadly neutralizing antibody 3/11, P21 crystal form~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Krey, T]]
		+	[[Category: Rey, F A]]
		+	[[Category: E2]]
		+	[[Category: Envelope glycoprotein]]
		+	[[Category: Neutralizing epitope]]
		+	[[Category: Receptor-binding]]
		+	[[Category: Viral protein]]

OCA: Protected "4why" [edit=sysop:move=sysop]

OCA — Wed, 01 Oct 2014 23:34:02 GMT

Protected "4why" [edit=sysop:move=sysop]

←Older revision

Revision as of 23:34, 1 October 2014

OCA: New page: '''Unreleased structure''' The entry 4why is ON HOLD Authors: Krey, T., Rey, F.A. Description: Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 boun...

OCA — Wed, 01 Oct 2014 23:34:00 GMT

New page: '''Unreleased structure''' The entry 4why is ON HOLD Authors: Krey, T., Rey, F.A. Description: Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 boun...

New page

'''Unreleased structure'''

The entry 4why is ON HOLD

Authors: Krey, T., Rey, F.A.

Description: Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 bound to the broadly neutralizing antibody 3/11, P21 crystal form