4wkm - Revision history

OCA at 07:32, 27 September 2023

OCA — Wed, 27 Sep 2023 07:32:29 GMT

←Older revision		Revision as of 07:32, 27 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4wkm]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_freundii_ATCC_8090_=_MTCC_1658_=_NBRC_12681 Citrobacter freundii ATCC 8090 = MTCC 1658 = NBRC 12681]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WKM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WKM FirstGlance]. <br>		<table><tr><td colspan='2'>[[4wkm]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_freundii_ATCC_8090_=_MTCC_1658_=_NBRC_12681 Citrobacter freundii ATCC 8090 = MTCC 1658 = NBRC 12681]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WKM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WKM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=API:2,6-DIAMINOPIMELIC+ACID'>API</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=FGA:GAMMA-D-GLUTAMIC+ACID'>FGA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MUB:N-ACETYLMURAMIC+ACID'>MUB</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=API:2,6-DIAMINOPIMELIC+ACID'>API</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=FGA:GAMMA-D-GLUTAMIC+ACID'>FGA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MUB:N-ACETYLMURAMIC+ACID'>MUB</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wkm OCA], [https://pdbe.org/4wkm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wkm RCSB], [https://www.ebi.ac.uk/pdbsum/4wkm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wkm ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wkm OCA], [https://pdbe.org/4wkm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wkm RCSB], [https://www.ebi.ac.uk/pdbsum/4wkm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wkm ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:13, 7 April 2023

OCA — Fri, 07 Apr 2023 07:13:05 GMT

←Older revision		Revision as of 07:13, 7 April 2023
Line 3:		Line 3:
	<StructureSection load='4wkm' size='340' side='right'caption='[[4wkm]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4wkm' size='340' side='right'caption='[[4wkm]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4wkm]] is a 16 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Citrobacter_freundii_atcc_8090~~ Citrobacter freundii ~~atcc~~ 8090]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WKM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4WKM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4wkm]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_freundii_ATCC_8090_=_MTCC_1658_=_NBRC_12681 Citrobacter freundii ATCC 8090 = MTCC 1658 = NBRC 12681]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WKM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WKM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MUB:N-ACETYLMURAMIC+ACID'>MUB</scene></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=API:2,6-DIAMINOPIMELIC+ACID'>API</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=FGA:GAMMA-D-GLUTAMIC+ACID'>FGA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MUB:N-ACETYLMURAMIC+ACID'>MUB</scene></td></tr>
-	~~<tr~~ id~~='NonStdRes'><td class~~="~~sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat~~"><scene name='pdbligand=API:2,6-DIAMINOPIMELIC+ACID'>API</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=FGA:GAMMA-D-GLUTAMIC+ACID'>FGA</scene><~~/td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wkm OCA], [https://pdbe.org/4wkm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wkm RCSB], [https://www.ebi.ac.uk/pdbsum/4wkm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wkm ProSAT]</span></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td>~~<td class="sblockDat">[[3kos\|3kos]]~~, ~~[[3kot\|3kot]]~~<~~/td></tr>~~	+
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">D186_13659 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1006003 Citrobacter freundii ATCC 8090])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4wkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wkm OCA], [~~http~~://pdbe.org/4wkm PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4wkm RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4wkm PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4wkm ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 18:

	==See Also==		==See Also==
-	*[[Transcriptional activator\|Transcriptional activator]]	+	*[[Transcriptional activator 3D structures\|Transcriptional activator 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Citrobacter freundii ~~atcc~~ 8090]]	+	[[Category: Citrobacter freundii ATCC 8090 = MTCC 1658 = NBRC 12681]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Mark~~, B L~~]]	+	[[Category: Mark BL]]
-	[[Category: Reeve~~, T M~~]]	+	[[Category: Reeve TM]]
-	[[Category: Vadlamani, G]]	+	[[Category: Vadlamani G]]
-	~~[[Category: Lttr]]~~	+
-	~~[[Category: Lysr-type transcriptional regulator]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Udp-murnac-pentapeptide~~]]	+

OCA at 08:43, 23 May 2019

OCA — Thu, 23 May 2019 08:43:41 GMT

←Older revision		Revision as of 08:43, 23 May 2019
Line 1:		Line 1:

	==AmpR effector binding domain from Citrobacter freundii bound to UDP-MurNAc-pentapeptide==		==AmpR effector binding domain from Citrobacter freundii bound to UDP-MurNAc-pentapeptide==
-	<StructureSection load='4wkm' size='340' side='right' caption='[[4wkm]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='4wkm' size='340' side='right'caption='[[4wkm]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4wkm]] is a 16 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WKM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WKM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4wkm]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Citrobacter_freundii_atcc_8090 Citrobacter freundii atcc 8090]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WKM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WKM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MUB:N-ACETYLMURAMIC+ACID'>MUB</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MUB:N-ACETYLMURAMIC+ACID'>MUB</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=API:2,6-DIAMINOPIMELIC+ACID'>API</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=FGA:GAMMA-D-GLUTAMIC+ACID'>FGA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=API:2,6-DIAMINOPIMELIC+ACID'>API</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=FGA:GAMMA-D-GLUTAMIC+ACID'>FGA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3kos\|3kos]], [[3kot\|3kot]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3kos\|3kos]], [[3kot\|3kot]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">D186_13659 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1006003 Citrobacter freundii ATCC 8090])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wkm OCA], [http://pdbe.org/4wkm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wkm RCSB], [http://www.ebi.ac.uk/pdbsum/4wkm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wkm ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wkm OCA], [http://pdbe.org/4wkm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wkm RCSB], [http://www.ebi.ac.uk/pdbsum/4wkm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wkm ProSAT]</span></td></tr>
	</table>		</table>
Line 18:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 4wkm" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4wkm" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Transcriptional activator\|Transcriptional activator]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Citrobacter freundii atcc 8090]]
		+	[[Category: Large Structures]]
	[[Category: Mark, B L]]		[[Category: Mark, B L]]
	[[Category: Reeve, T M]]		[[Category: Reeve, T M]]

OCA at 00:12, 26 January 2017

OCA — Thu, 26 Jan 2017 00:12:33 GMT

←Older revision		Revision as of 00:12, 26 January 2017
Line 1:		Line 1:
		+
	==AmpR effector binding domain from Citrobacter freundii bound to UDP-MurNAc-pentapeptide==		==AmpR effector binding domain from Citrobacter freundii bound to UDP-MurNAc-pentapeptide==
	<StructureSection load='4wkm' size='340' side='right' caption='[[4wkm]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4wkm' size='340' side='right' caption='[[4wkm]], [[Resolution\|resolution]] 2.15Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=API:2,6-DIAMINOPIMELIC+ACID'>API</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=FGA:GAMMA-D-GLUTAMIC+ACID'>FGA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=API:2,6-DIAMINOPIMELIC+ACID'>API</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=FGA:GAMMA-D-GLUTAMIC+ACID'>FGA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3kos\|3kos]], [[3kot\|3kot]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3kos\|3kos]], [[3kot\|3kot]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wkm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wkm RCSB], [http://www.ebi.ac.uk/pdbsum/4wkm PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wkm OCA], [http://pdbe.org/4wkm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wkm RCSB], [http://www.ebi.ac.uk/pdbsum/4wkm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wkm ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4wkm" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:01, 12 February 2015

OCA — Thu, 12 Feb 2015 06:01:21 GMT

←Older revision		Revision as of 06:01, 12 February 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[4wkm]] is a 16 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WKM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WKM FirstGlance]. <br>		<table><tr><td colspan='2'>[[4wkm]] is a 16 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WKM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WKM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MUB:N-ACETYLMURAMIC+ACID'>MUB</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MUB:N-ACETYLMURAMIC+ACID'>MUB</scene></td></tr>
-	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=API:2,6-DIAMINOPIMELIC+ACID'>API</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=~~DGL~~:D-GLUTAMIC+ACID'>~~DGL~~</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=API:2,6-DIAMINOPIMELIC+ACID'>API</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=FGA:GAMMA-D-GLUTAMIC+ACID'>FGA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3kos\|3kos]], [[3kot\|3kot]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3kos\|3kos]], [[3kot\|3kot]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wkm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wkm RCSB], [http://www.ebi.ac.uk/pdbsum/4wkm PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wkm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wkm RCSB], [http://www.ebi.ac.uk/pdbsum/4wkm PDBsum]</span></td></tr>

OCA at 12:40, 17 December 2014

OCA — Wed, 17 Dec 2014 12:40:57 GMT

←Older revision		Revision as of 12:40, 17 December 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==AmpR effector binding domain from Citrobacter freundii bound to UDP-MurNAc-pentapeptide==
		+	<StructureSection load='4wkm' size='340' side='right' caption='[[4wkm]], [[Resolution\|resolution]] 2.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4wkm]] is a 16 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WKM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WKM FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MUB:N-ACETYLMURAMIC+ACID'>MUB</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=API:2,6-DIAMINOPIMELIC+ACID'>API</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=DGL:D-GLUTAMIC+ACID'>DGL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3kos\|3kos]], [[3kot\|3kot]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wkm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wkm RCSB], [http://www.ebi.ac.uk/pdbsum/4wkm PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Inducible expression of chromosomal AmpC beta-lactamase is a major cause of beta-lactam antibiotic resistance in the Gram negative bacteria Pseudomonas aeruginosa and Enterobacteriaceae. AmpC expression is induced by the LysR-type transcriptional regulator (LTTR) AmpR, which activates ampC expression in response to changes in peptidoglycan (PG) metabolite levels that occur during exposure to beta-lactams. Under normal conditions, AmpR represses ampC transcription by binding the PG precursor UDP-MurNAc-pentapeptide. When exposed to beta-lactams however, PG catabolites (1,6-anhydroMurNAc-peptides) accumulate in the cytosol, which have been proposed to competitively displace UDP-MurNAcpentapeptide from AmpR and convert it into an activator of ampC transcription. Here we describe the molecular interactions between AmpR (from Citrobacter freundii), its DNA operator, and repressor UDP-MurNAcpentapeptide. Non-denaturing mass spectrometry revealed AmpR to be a homotetramer that is stabilized by DNA containing the T-N11-A LTTR binding motif, and that it can bind four repressor molecules in an apparently stepwise manner. A crystal structure of the AmpR effector-binding domain bound to UDP-MurNAc-pentapeptide revealed that the terminal D-Ala-D-Ala motif of the repressor forms the primary contacts with the protein. This observation supports previous claims that 1,6-anhydro-MurNAc-pentapeptide converts AmpR into an activator of ampC transcription more effectively than 1,6-anhydro-MurNAc-tripeptide (which lacks the D-Ala-D-Ala motif). Finally, small angle X-ray scattering demonstrates that the AmpR-DNA complex adopts a flat conformation similar to the LTTR protein AphB and undergoes only a slight conformational change when binding UDP-MurNAc-pentapeptide. Modeling the AmpR:DNA tetramer bound to UDP-MurNAcpentapeptide predicts that the UDP-MurNAc moiety of the repressor participates in modulating AmpR function.

-	The ~~entry 4wkm~~ is ~~ON HOLD until Paper Publication~~	+	The beta-lactamase Gene Regulator AmpR is a Tetramer that Recognizes and Binds the D-Ala-D-Ala Motif of its Repressor UDP-MurNAc-pentapeptide.,Vadlamani G, Thomas MD, Patel TR, Donald LJ, Reeve TM, Stetefeld J, Standing KG, Vocadlo DJ, Mark BL J Biol Chem. 2014 Dec 5. pii: jbc.M114.618199. PMID:25480792<ref>PMID:25480792</ref>

-	~~Authors: Vadlamani~~, G.~~, Reeve, T~~.M., Mark, B.L.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~AmpR effector binding domain from Citrobacter freundii bound to UDP~~-~~MurNAc~~-pentapeptide	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Mark, B L]]
		+	[[Category: Reeve, T M]]
		+	[[Category: Vadlamani, G]]
		+	[[Category: Lttr]]
		+	[[Category: Lysr-type transcriptional regulator]]
		+	[[Category: Transcription]]
		+	[[Category: Udp-murnac-pentapeptide]]

OCA at 08:35, 3 December 2014

OCA — Wed, 03 Dec 2014 08:35:03 GMT

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-	The entry 4wkm is ON HOLD	+	The entry 4wkm is ON HOLD until Paper Publication

	Authors: Vadlamani, G., Reeve, T.M., Mark, B.L.		Authors: Vadlamani, G., Reeve, T.M., Mark, B.L.

	Description: AmpR effector binding domain from Citrobacter freundii bound to UDP-MurNAc-pentapeptide		Description: AmpR effector binding domain from Citrobacter freundii bound to UDP-MurNAc-pentapeptide

OCA: Protected "4wkm" [edit=sysop:move=sysop]

OCA — Wed, 08 Oct 2014 06:59:41 GMT

Protected "4wkm" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:59, 8 October 2014

OCA: New page: '''Unreleased structure''' The entry 4wkm is ON HOLD Authors: Vadlamani, G., Reeve, T.M., Mark, B.L. Description: AmpR effector binding domain from Citrobacter freundii bound to UDP-Mu...

OCA — Wed, 08 Oct 2014 06:59:40 GMT

New page: '''Unreleased structure''' The entry 4wkm is ON HOLD Authors: Vadlamani, G., Reeve, T.M., Mark, B.L. Description: AmpR effector binding domain from Citrobacter freundii bound to UDP-Mu...

New page

'''Unreleased structure'''

The entry 4wkm is ON HOLD

Authors: Vadlamani, G., Reeve, T.M., Mark, B.L.

Description: AmpR effector binding domain from Citrobacter freundii bound to UDP-MurNAc-pentapeptide