4wnl - Revision history

OCA at 00:54, 28 December 2023

OCA — Thu, 28 Dec 2023 00:54:29 GMT

←Older revision		Revision as of 00:54, 28 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4wnl]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_AWRI1631 Saccharomyces cerevisiae AWRI1631] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WNL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WNL FirstGlance]. <br>		<table><tr><td colspan='2'>[[4wnl]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_AWRI1631 Saccharomyces cerevisiae AWRI1631] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WNL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WNL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wnl OCA], [https://pdbe.org/4wnl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wnl RCSB], [https://www.ebi.ac.uk/pdbsum/4wnl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wnl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wnl OCA], [https://pdbe.org/4wnl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wnl RCSB], [https://www.ebi.ac.uk/pdbsum/4wnl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wnl ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:17, 7 April 2023

OCA — Fri, 07 Apr 2023 07:17:12 GMT

←Older revision		Revision as of 07:17, 7 April 2023
Line 3:		Line 3:
	<StructureSection load='4wnl' size='340' side='right'caption='[[4wnl]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='4wnl' size='340' side='right'caption='[[4wnl]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4wnl]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WNL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4WNL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4wnl]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_AWRI1631 Saccharomyces cerevisiae AWRI1631] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WNL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WNL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xly\|1xly]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wnl OCA], [https://pdbe.org/4wnl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wnl RCSB], [https://www.ebi.ac.uk/pdbsum/4wnl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wnl ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SHE2, YKL130C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4wnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wnl OCA], [~~http~~://pdbe.org/4wnl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4wnl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4wnl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4wnl ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SHE2_YEAST SHE2_YEAST]] RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Recruits the MYO4-SHE3 complex to the ASH1 mRNA. Recruites also LOC1 and PUF6 to ASH1 mRNA, which are required for translational repression of this mRNA.<ref>PMID:10212145</ref> <ref>PMID:10359695</ref> <ref>PMID:11032818</ref> <ref>PMID:11101531</ref> <ref>PMID:12499354</ref> <ref>PMID:13679573</ref> <ref>PMID:14561888</ref> <ref>PMID:14691136</ref> <ref>PMID:15328357</ref> <ref>PMID:15537539</ref> <ref>PMID:15899876</ref> <ref>PMID:16890529</ref> <ref>PMID:18566598</ref> <ref>PMID:19244342</ref> <ref>PMID:20713510</ref> <ref>PMID:9809065</ref> [[http://www.uniprot.org/uniprot/SHE3_YEAS6 SHE3_YEAS6]] RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Required for the delivery of cortical endoplasmic reticulum into the emerging bud (By similarity).	+	[https://www.uniprot.org/uniprot/SHE2_YEAST SHE2_YEAST] RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Recruits the MYO4-SHE3 complex to the ASH1 mRNA. Recruites also LOC1 and PUF6 to ASH1 mRNA, which are required for translational repression of this mRNA.<ref>PMID:10212145</ref> <ref>PMID:10359695</ref> <ref>PMID:11032818</ref> <ref>PMID:11101531</ref> <ref>PMID:12499354</ref> <ref>PMID:13679573</ref> <ref>PMID:14561888</ref> <ref>PMID:14691136</ref> <ref>PMID:15328357</ref> <ref>PMID:15537539</ref> <ref>PMID:15899876</ref> <ref>PMID:16890529</ref> <ref>PMID:18566598</ref> <ref>PMID:19244342</ref> <ref>PMID:20713510</ref> <ref>PMID:9809065</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Baker's yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Blobel, G~~]]	+	[[Category: Saccharomyces cerevisiae AWRI1631]]
-	[[Category: ~~Shi, H~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: ~~Singh, N~~]]	+	[[Category: Blobel G]]
-	[[Category: ~~Mrna transport~~]]	+	[[Category: Shi H]]
-	[[Category: ~~Rna binding protein]]~~	+	[[Category: Singh N]]
-	~~[[Category: Rna-binding~~]]	+

OCA at 08:40, 4 December 2019

OCA — Wed, 04 Dec 2019 08:40:58 GMT

←Older revision		Revision as of 08:40, 4 December 2019
Line 3:		Line 3:
	<StructureSection load='4wnl' size='340' side='right'caption='[[4wnl]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='4wnl' size='340' side='right'caption='[[4wnl]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4wnl]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WNL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WNL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4wnl]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WNL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WNL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xly\|1xly]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xly\|1xly]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SHE2, YKL130C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wnl OCA], [http://pdbe.org/4wnl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wnl RCSB], [http://www.ebi.ac.uk/pdbsum/4wnl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wnl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wnl OCA], [http://pdbe.org/4wnl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wnl RCSB], [http://www.ebi.ac.uk/pdbsum/4wnl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wnl ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Baker's yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Blobel, G]]		[[Category: Blobel, G]]

OCA at 07:17, 10 July 2019

OCA — Wed, 10 Jul 2019 07:17:57 GMT

←Older revision		Revision as of 07:17, 10 July 2019
Line 1:		Line 1:

	==The X-ray structure of a RNA-binding protein complex==		==The X-ray structure of a RNA-binding protein complex==
-	<StructureSection load='4wnl' size='340' side='right' caption='[[4wnl]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='4wnl' size='340' side='right'caption='[[4wnl]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4wnl]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WNL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WNL FirstGlance]. <br>		<table><tr><td colspan='2'>[[4wnl]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WNL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WNL FirstGlance]. <br>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Blobel, G]]		[[Category: Blobel, G]]
	[[Category: Shi, H]]		[[Category: Shi, H]]

OCA at 08:42, 11 August 2016

OCA — Thu, 11 Aug 2016 08:42:54 GMT

←Older revision		Revision as of 08:42, 11 August 2016
Line 1:		Line 1:
		+
	==The X-ray structure of a RNA-binding protein complex==		==The X-ray structure of a RNA-binding protein complex==
	<StructureSection load='4wnl' size='340' side='right' caption='[[4wnl]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='4wnl' size='340' side='right' caption='[[4wnl]], [[Resolution\|resolution]] 2.80Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xly\|1xly]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xly\|1xly]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wnl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wnl RCSB], [http://www.ebi.ac.uk/pdbsum/4wnl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wnl OCA], [http://pdbe.org/4wnl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wnl RCSB], [http://www.ebi.ac.uk/pdbsum/4wnl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wnl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4wnl" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:42, 14 January 2015

OCA — Wed, 14 Jan 2015 07:42:53 GMT

←Older revision		Revision as of 07:42, 14 January 2015
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/SHE2_YEAST SHE2_YEAST]] RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Recruits the MYO4-SHE3 complex to the ASH1 mRNA. Recruites also LOC1 and PUF6 to ASH1 mRNA, which are required for translational repression of this mRNA.<ref>PMID:10212145</ref> <ref>PMID:10359695</ref> <ref>PMID:11032818</ref> <ref>PMID:11101531</ref> <ref>PMID:12499354</ref> <ref>PMID:13679573</ref> <ref>PMID:14561888</ref> <ref>PMID:14691136</ref> <ref>PMID:15328357</ref> <ref>PMID:15537539</ref> <ref>PMID:15899876</ref> <ref>PMID:16890529</ref> <ref>PMID:18566598</ref> <ref>PMID:19244342</ref> <ref>PMID:20713510</ref> <ref>PMID:9809065</ref> [[http://www.uniprot.org/uniprot/SHE3_YEAS6 SHE3_YEAS6]] RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Required for the delivery of cortical endoplasmic reticulum into the emerging bud (By similarity).		[[http://www.uniprot.org/uniprot/SHE2_YEAST SHE2_YEAST]] RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Recruits the MYO4-SHE3 complex to the ASH1 mRNA. Recruites also LOC1 and PUF6 to ASH1 mRNA, which are required for translational repression of this mRNA.<ref>PMID:10212145</ref> <ref>PMID:10359695</ref> <ref>PMID:11032818</ref> <ref>PMID:11101531</ref> <ref>PMID:12499354</ref> <ref>PMID:13679573</ref> <ref>PMID:14561888</ref> <ref>PMID:14691136</ref> <ref>PMID:15328357</ref> <ref>PMID:15537539</ref> <ref>PMID:15899876</ref> <ref>PMID:16890529</ref> <ref>PMID:18566598</ref> <ref>PMID:19244342</ref> <ref>PMID:20713510</ref> <ref>PMID:9809065</ref> [[http://www.uniprot.org/uniprot/SHE3_YEAS6 SHE3_YEAS6]] RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Required for the delivery of cortical endoplasmic reticulum into the emerging bud (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The segregation of approximately two dozen distinct mRNAs from yeast mother to daughter cell cytoplasm is a classical paradigm for eukaryotic mRNA transport. The information for transport resides in an mRNA element 40-100 nt in length, known as "zipcode." Targeted transport requires properly positioned actin filaments and cooperative loading of mRNA cargo to myosin. Cargo loading to myosin uses myosin 4 protein (Myo4p), swi5p-dependent HO expression 2 protein (She2p) and 3 protein (She3p), and zipcode. We previously determined a crystal structure of Myo4p and She3p, their 1:2 stoichiometry and interactome; we furthermore showed that the motor complex assembly requires two Myo4pShe3p heterotrimers, one She2p tetramer, and at least a single zipcode to yield a stable complex of [Myo4pShe3pShe2pzipcode] in 2:4:4:1 stoichiometry in vitro. Here, we report a structure at 2.8-A resolution of a cocrystal of a She2p tetramer bound to a segment of She3p. In this crystal structure, the She3p segment forms a striking hook that binds to a shallow hydrophobic pocket on the surface of each She2p subunit of the tetramer. Both She3p hook and cognate She2p binding pocket are composed of highly conserved residues. We also discovered a highly conserved region of She3p upstream of its hook region. Because this region consists of basic and aromatic residues, it likely represents part of She3p's binding activity for zipcode. Because She2p also exhibits zipcode-binding activity, we suggest that "hooking" She3p onto She2p aligns each of their zipcode-binding activities into a high-affinity site, thereby linking motor assembly to zipcode.
		+
		+	Hooking She3p onto She2p for myosin-mediated cytoplasmic mRNA transport.,Singh N, Blobel G, Shi H Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):142-7. doi: 10.1073/pnas.1423194112. , Epub 2014 Dec 22. PMID:25535369<ref>PMID:25535369</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:10, 24 December 2014

OCA — Wed, 24 Dec 2014 08:10:33 GMT

←Older revision		Revision as of 08:10, 24 December 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==The X-ray structure of a RNA-binding protein complex==
-		+	<StructureSection load='4wnl' size='340' side='right' caption='[[4wnl]], [[Resolution\|resolution]] 2.80Å' scene=''>
-	~~The entry~~ 4wnl is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4wnl]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WNL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WNL FirstGlance]. <br>
-	~~Authors~~: ~~Singh~~, N., ~~Blobel~~, G., Shi, H.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xly\|1xly]]</td></tr>
-	~~Description~~:	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wnl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wnl RCSB], [http://www.ebi.ac.uk/pdbsum/4wnl PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SHE2_YEAST SHE2_YEAST]] RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Recruits the MYO4-SHE3 complex to the ASH1 mRNA. Recruites also LOC1 and PUF6 to ASH1 mRNA, which are required for translational repression of this mRNA.<ref>PMID:10212145</ref> <ref>PMID:10359695</ref> <ref>PMID:11032818</ref> <ref>PMID:11101531</ref> <ref>PMID:12499354</ref> <ref>PMID:13679573</ref> <ref>PMID:14561888</ref> <ref>PMID:14691136</ref> <ref>PMID:15328357</ref> <ref>PMID:15537539</ref> <ref>PMID:15899876</ref> <ref>PMID:16890529</ref> <ref>PMID:18566598</ref> <ref>PMID:19244342</ref> <ref>PMID:20713510</ref> <ref>PMID:9809065</ref> [[http://www.uniprot.org/uniprot/SHE3_YEAS6 SHE3_YEAS6]] RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Required for the delivery of cortical endoplasmic reticulum into the emerging bud (By similarity).
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Blobel, G]]
		+	[[Category: Shi, H]]
		+	[[Category: Singh, N]]
		+	[[Category: Mrna transport]]
		+	[[Category: Rna binding protein]]
		+	[[Category: Rna-binding]]

OCA at 10:32, 29 October 2014

OCA — Wed, 29 Oct 2014 10:32:20 GMT

←Older revision		Revision as of 10:32, 29 October 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4wnl is ON HOLD	+	The entry 4wnl is ON HOLD until Paper Publication

	Authors: Singh, N., Blobel, G., Shi, H.		Authors: Singh, N., Blobel, G., Shi, H.

	Description:		Description:

OCA: Protected "4wnl" [edit=sysop:move=sysop]

OCA — Wed, 22 Oct 2014 10:21:34 GMT

Protected "4wnl" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:21, 22 October 2014

OCA: New page: '''Unreleased structure''' The entry 4wnl is ON HOLD Authors: Singh, N., Blobel, G., Shi, H. Description:

OCA — Wed, 22 Oct 2014 10:21:32 GMT

New page: '''Unreleased structure''' The entry 4wnl is ON HOLD Authors: Singh, N., Blobel, G., Shi, H. Description:

New page

'''Unreleased structure'''

The entry 4wnl is ON HOLD

Authors: Singh, N., Blobel, G., Shi, H.

Description: