4wtp - Revision history

OCA at 07:26, 7 April 2023

OCA — Fri, 07 Apr 2023 07:26:21 GMT

←Older revision		Revision as of 07:26, 7 April 2023
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	<StructureSection load='4wtp' size='340' side='right'caption='[[4wtp]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='4wtp' size='340' side='right'caption='[[4wtp]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4wtp]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Rhizomucor_miehei_cau432~~ Rhizomucor miehei ~~cau432~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WTP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4WTP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4wtp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizomucor_miehei_CAU432 Rhizomucor miehei CAU432]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WTP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wtr\|4wtr]], [[4wts\|4wts]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wtp OCA], [https://pdbe.org/4wtp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wtp RCSB], [https://www.ebi.ac.uk/pdbsum/4wtp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wtp ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4wtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wtp OCA], [~~http~~://pdbe.org/4wtp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4wtp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4wtp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4wtp ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0M3KKZ6_RHIMI A0A0M3KKZ6_RHIMI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Rhizomucor miehei ~~cau432~~]]	+	[[Category: Rhizomucor miehei CAU432]]
-	[[Category: Jiang, Z]]	+	[[Category: Jiang Z]]
-	[[Category: Lei, J]]	+	[[Category: Lei J]]
-	[[Category: Qin, Z]]	+	[[Category: Qin Z]]
-	[[Category: Yan, Q]]	+	[[Category: Yan Q]]
-	[[Category: Yang, S]]	+	[[Category: Yang S]]
-	~~[[Category: 3-glucanosyltransferase]]~~	+
-	~~[[Category: Beta-1]]~~	+
-	~~[[Category: Glycoside hydrolases family 17]]~~	+
-	~~[[Category: Rhizomucor miehei]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Transglycosylation~~]]	+

OCA at 10:31, 25 December 2019

OCA — Wed, 25 Dec 2019 10:31:16 GMT

←Older revision		Revision as of 10:31, 25 December 2019
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wtp OCA], [http://pdbe.org/4wtp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wtp RCSB], [http://www.ebi.ac.uk/pdbsum/4wtp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wtp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wtp OCA], [http://pdbe.org/4wtp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wtp RCSB], [http://www.ebi.ac.uk/pdbsum/4wtp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wtp ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	beta-1,3-Glucanosyltransferase (EC 2.4.1.-) plays an important role in the formation of branched glucans, as well as in cell-wall assembly and rearrangement in fungi and yeasts. The crystal structures of a novel glycoside hydrolase (GH) family 17 beta-1,3-glucanosyltransferase from Rhizomucor miehei (RmBgt17A) and the complexes of its active-site mutant (E189A) with two substrates were solved at resolutions of 1.30, 2.30 and 2.27 A, respectively. The overall structure of RmBgt17A had the characteristic (beta/alpha)8 TIM-barrel fold. The structures of RmBgt17A and other GH family 17 members were compared: it was found that a conserved subdomain located in the region near helix alpha6 and part of the catalytic cleft in other GH family 17 members was absent in RmBgt17A. Instead, four amino-acid residues exposed to the surface of the enzyme (Tyr135, Tyr136, Glu158 and His172) were found in the reducing terminus of subsite +2 of RmBgt17A, hindering access to the catalytic cleft. This distinct region of RmBgt17A makes its catalytic cleft shorter than those of other reported GH family 17 enzymes. The complex structures also illustrated that RmBgt17A can only provide subsites -3 to +2. This structural evidence provides a clear explanation of the catalytic mode of RmBgt17A, in which laminaribiose is released from the reducing end of linear beta-1,3-glucan and the remaining glucan is transferred to the end of another beta-1,3-glucan acceptor. The first crystal structure of a GH family 17 beta-1,3-glucanosyltransferase may be useful in studies of the catalytic mechanism of GH family 17 proteins, and provides a basis for further enzymatic engineering or antifungal drug screening.
		+
		+	The first crystal structure of a glycoside hydrolase family 17 beta-1,3-glucanosyltransferase displays a unique catalytic cleft.,Qin Z, Yan Q, Lei J, Yang S, Jiang Z, Wu S Acta Crystallogr D Biol Crystallogr. 2015 Aug;71(Pt 8):1714-24. doi:, 10.1107/S1399004715011037. Epub 2015 Jul 31. PMID:26249352<ref>PMID:26249352</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4wtp" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:54, 23 May 2019

OCA — Thu, 23 May 2019 07:54:49 GMT

←Older revision		Revision as of 07:54, 23 May 2019
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	==Crystal structure of glycoside hydrolase family 17 beta-1,3-glucanosyltransferase from Rhizomucor miehei==		==Crystal structure of glycoside hydrolase family 17 beta-1,3-glucanosyltransferase from Rhizomucor miehei==
-	<StructureSection load='4wtp' size='340' side='right' caption='[[4wtp]], [[Resolution\|resolution]] 1.30Å' scene=''>	+	<StructureSection load='4wtp' size='340' side='right'caption='[[4wtp]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4wtp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WTP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WTP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4wtp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhizomucor_miehei_cau432 Rhizomucor miehei cau432]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WTP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WTP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wtr\|4wtr]], [[4wts\|4wts]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wtr\|4wtr]], [[4wts\|4wts]]</td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Rhizomucor miehei cau432]]
	[[Category: Jiang, Z]]		[[Category: Jiang, Z]]
	[[Category: Lei, J]]		[[Category: Lei, J]]

OCA at 16:06, 17 May 2017

OCA — Wed, 17 May 2017 16:06:51 GMT

←Older revision		Revision as of 16:06, 17 May 2017
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		+
	==Crystal structure of glycoside hydrolase family 17 beta-1,3-glucanosyltransferase from Rhizomucor miehei==		==Crystal structure of glycoside hydrolase family 17 beta-1,3-glucanosyltransferase from Rhizomucor miehei==
	<StructureSection load='4wtp' size='340' side='right' caption='[[4wtp]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='4wtp' size='340' side='right' caption='[[4wtp]], [[Resolution\|resolution]] 1.30Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wtr\|4wtr]], [[4wts\|4wts]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wtr\|4wtr]], [[4wts\|4wts]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wtp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wtp RCSB], [http://www.ebi.ac.uk/pdbsum/4wtp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wtp OCA], [http://pdbe.org/4wtp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wtp RCSB], [http://www.ebi.ac.uk/pdbsum/4wtp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wtp ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 14:48, 12 August 2015

OCA — Wed, 12 Aug 2015 14:48:42 GMT

←Older revision		Revision as of 14:48, 12 August 2015
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-	'''~~Unreleased structure~~'''	+	==Crystal structure of glycoside hydrolase family 17 beta-1,3-glucanosyltransferase from Rhizomucor miehei==
-		+	<StructureSection load='4wtp' size='340' side='right' caption='[[4wtp]], [[Resolution\|resolution]] 1.30Å' scene=''>
-	~~The entry~~ 4wtp ~~is ON HOLD until Oct~~ 30 ~~2016~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4wtp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WTP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WTP FirstGlance]. <br>
-	~~Authors~~: ~~Qin, Z~~.~~, Yan, Q~~.~~, Lei, J~~.~~, Yang, S~~.~~, Jiang, Z~~.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wtr\|4wtr]], [[4wts\|4wts]]</td></tr>
-	~~Description~~: ~~Crystal structure of glycoside hydrolase family 17 beta-~~1,3-~~glucanosyltransferase from Rhizomucor miehei~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wtp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wtp RCSB], [http://www.ebi.ac.uk/pdbsum/4wtp PDBsum]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
	[[Category: Jiang, Z]]		[[Category: Jiang, Z]]
		+	[[Category: Lei, J]]
	[[Category: Qin, Z]]		[[Category: Qin, Z]]
-	[[Category: Yang, S]]
	[[Category: Yan, Q]]		[[Category: Yan, Q]]
-	[[Category: ~~Lei~~, J]]	+	[[Category: Yang, S]]
		+	[[Category: 3-glucanosyltransferase]]
		+	[[Category: Beta-1]]
		+	[[Category: Glycoside hydrolases family 17]]
		+	[[Category: Rhizomucor miehei]]
		+	[[Category: Transferase]]
		+	[[Category: Transglycosylation]]

OCA at 08:17, 8 April 2015

OCA — Wed, 08 Apr 2015 08:17:02 GMT

←Older revision		Revision as of 08:17, 8 April 2015
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4wtp is ON HOLD	+	The entry 4wtp is ON HOLD until Oct 30 2016

	Authors: Qin, Z., Yan, Q., Lei, J., Yang, S., Jiang, Z.		Authors: Qin, Z., Yan, Q., Lei, J., Yang, S., Jiang, Z.

OCA at 19:58, 24 December 2014

OCA — Wed, 24 Dec 2014 19:58:11 GMT

←Older revision		Revision as of 19:58, 24 December 2014
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	Description: Crystal structure of glycoside hydrolase family 17 beta-1,3-glucanosyltransferase from Rhizomucor miehei		Description: Crystal structure of glycoside hydrolase family 17 beta-1,3-glucanosyltransferase from Rhizomucor miehei
		+	[[Category: Unreleased Structures]]
		+	[[Category: Jiang, Z]]
		+	[[Category: Qin, Z]]
		+	[[Category: Yang, S]]
		+	[[Category: Yan, Q]]
		+	[[Category: Lei, J]]

OCA at 11:57, 19 November 2014

OCA — Wed, 19 Nov 2014 11:57:34 GMT

←Older revision		Revision as of 11:57, 19 November 2014
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	The entry 4wtp is ON HOLD		The entry 4wtp is ON HOLD

-	Authors: ~~Zhen~~ Qin, ~~Qiaojuan~~ Yan, ~~Jian lei~~, ~~Shaoqing~~ Yang, ~~Zhengqiang~~ Jiang	+	Authors: Qin, Z., Yan, Q., Lei, J., Yang, S., Jiang, Z.

	Description: Crystal structure of glycoside hydrolase family 17 beta-1,3-glucanosyltransferase from Rhizomucor miehei		Description: Crystal structure of glycoside hydrolase family 17 beta-1,3-glucanosyltransferase from Rhizomucor miehei

OCA: Protected "4wtp" [edit=sysop:move=sysop]

OCA — Wed, 05 Nov 2014 08:09:12 GMT

Protected "4wtp" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:09, 5 November 2014

OCA: New page: '''Unreleased structure''' The entry 4wtp is ON HOLD Authors: Zhen Qin, Qiaojuan Yan, Jian lei, Shaoqing Yang, Zhengqiang Jiang Description: Crystal structure of glycoside hydrolase fa...

OCA — Wed, 05 Nov 2014 08:09:11 GMT

New page: '''Unreleased structure''' The entry 4wtp is ON HOLD Authors: Zhen Qin, Qiaojuan Yan, Jian lei, Shaoqing Yang, Zhengqiang Jiang Description: Crystal structure of glycoside hydrolase fa...

New page

'''Unreleased structure'''

The entry 4wtp is ON HOLD

Authors: Zhen Qin, Qiaojuan Yan, Jian lei, Shaoqing Yang, Zhengqiang Jiang

Description: Crystal structure of glycoside hydrolase family 17 beta-1,3-glucanosyltransferase from Rhizomucor miehei