4wx1 - Revision history

OCA at 07:38, 27 September 2023

OCA — Wed, 27 Sep 2023 07:38:20 GMT

←Older revision		Revision as of 07:38, 27 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4wx1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WX1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WX1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4wx1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WX1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WX1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wx1 OCA], [https://pdbe.org/4wx1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wx1 RCSB], [https://www.ebi.ac.uk/pdbsum/4wx1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wx1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wx1 OCA], [https://pdbe.org/4wx1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wx1 RCSB], [https://www.ebi.ac.uk/pdbsum/4wx1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wx1 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 21:01, 12 April 2023

OCA — Wed, 12 Apr 2023 21:01:19 GMT

←Older revision		Revision as of 21:01, 12 April 2023
Line 3:		Line 3:
	<StructureSection load='4wx1' size='340' side='right'caption='[[4wx1]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4wx1' size='340' side='right'caption='[[4wx1]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4wx1]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aspfm Aspfm~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WX1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4WX1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4wx1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WX1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WX1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">glf, glfA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746128 ASPFM])</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wx1 OCA], [https://pdbe.org/4wx1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wx1 RCSB], [https://www.ebi.ac.uk/pdbsum/4wx1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wx1 ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-galactopyranose_mutase UDP-galactopyranose mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.9 5.4.99.9] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4wx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wx1 OCA], [~~http~~://pdbe.org/4wx1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4wx1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4wx1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4wx1 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q4W1X2_ASPFM Q4W1X2_ASPFM]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[UDP-galactopyranose mutase\|UDP-galactopyranose mutase]]	+	*[[UDP-galactopyranose mutase 3D structures\|UDP-galactopyranose mutase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aspfm~~]]	+	[[Category: Aspergillus fumigatus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: UDP-galactopyranose mutase]]~~	+	[[Category: Chaudhary R]]
-	[[Category: Chaudhary, R]]	+	[[Category: Qureshi IA]]
-	[[Category: Qureshi~~, I A]]~~	+
-	~~[[Category: Flavin adenine dinucleotide binding]]~~	+
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Mutase]]~~	+
-	~~[[Category: Nucleotide binding~~]]	+

OCA at 10:31, 25 December 2019

OCA — Wed, 25 Dec 2019 10:31:49 GMT

←Older revision		Revision as of 10:31, 25 December 2019
Line 1:		Line 1:

	==Structure of Aspergillus fumigatus UDP-Galactopyranose mutase mutant F66A determined from a crystal soaked with UDP-Galactopyranose==		==Structure of Aspergillus fumigatus UDP-Galactopyranose mutase mutant F66A determined from a crystal soaked with UDP-Galactopyranose==
-	<StructureSection load='4wx1' size='340' side='right' caption='[[4wx1]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='4wx1' size='340' side='right'caption='[[4wx1]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4wx1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspfm Aspfm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WX1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4wx1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspfm Aspfm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WX1 FirstGlance]. <br>
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4wx1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4wx1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[UDP-galactopyranose mutase\|UDP-galactopyranose mutase]]
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Aspfm]]		[[Category: Aspfm]]
		+	[[Category: Large Structures]]
	[[Category: UDP-galactopyranose mutase]]		[[Category: UDP-galactopyranose mutase]]
	[[Category: Chaudhary, R]]		[[Category: Chaudhary, R]]

OCA at 09:30, 22 November 2017

OCA — Wed, 22 Nov 2017 09:30:14 GMT

←Older revision		Revision as of 09:30, 22 November 2017
Line 3:		Line 3:
	<StructureSection load='4wx1' size='340' side='right' caption='[[4wx1]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4wx1' size='340' side='right' caption='[[4wx1]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4wx1]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WX1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4wx1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspfm Aspfm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WX1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">glf, glfA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746128 ASPFM])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-galactopyranose_mutase UDP-galactopyranose mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.9 5.4.99.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-galactopyranose_mutase UDP-galactopyranose mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.9 5.4.99.9] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wx1 OCA], [http://pdbe.org/4wx1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wx1 RCSB], [http://www.ebi.ac.uk/pdbsum/4wx1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wx1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wx1 OCA], [http://pdbe.org/4wx1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wx1 RCSB], [http://www.ebi.ac.uk/pdbsum/4wx1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wx1 ProSAT]</span></td></tr>
Line 17:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4wx1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4wx1" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[UDP-galactopyranose mutase\|UDP-galactopyranose mutase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aspfm]]
	[[Category: UDP-galactopyranose mutase]]		[[Category: UDP-galactopyranose mutase]]
	[[Category: Chaudhary, R]]		[[Category: Chaudhary, R]]

OCA at 07:48, 4 August 2016

OCA — Thu, 04 Aug 2016 07:48:41 GMT

←Older revision		Revision as of 07:48, 4 August 2016
Line 1:		Line 1:
		+
	==Structure of Aspergillus fumigatus UDP-Galactopyranose mutase mutant F66A determined from a crystal soaked with UDP-Galactopyranose==		==Structure of Aspergillus fumigatus UDP-Galactopyranose mutase mutant F66A determined from a crystal soaked with UDP-Galactopyranose==
	<StructureSection load='4wx1' size='340' side='right' caption='[[4wx1]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4wx1' size='340' side='right' caption='[[4wx1]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-galactopyranose_mutase UDP-galactopyranose mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.9 5.4.99.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-galactopyranose_mutase UDP-galactopyranose mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.9 5.4.99.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wx1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wx1 RCSB], [http://www.ebi.ac.uk/pdbsum/4wx1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wx1 OCA], [http://pdbe.org/4wx1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wx1 RCSB], [http://www.ebi.ac.uk/pdbsum/4wx1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wx1 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4wx1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[UDP-galactopyranose mutase\|UDP-galactopyranose mutase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:31, 3 December 2014

OCA — Wed, 03 Dec 2014 09:31:13 GMT

←Older revision		Revision as of 09:31, 3 December 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Structure of Aspergillus fumigatus UDP-Galactopyranose mutase mutant F66A determined from a crystal soaked with UDP-Galactopyranose==
		+	<StructureSection load='4wx1' size='340' side='right' caption='[[4wx1]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4wx1]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WX1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-galactopyranose_mutase UDP-galactopyranose mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.9 5.4.99.9] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wx1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wx1 RCSB], [http://www.ebi.ac.uk/pdbsum/4wx1 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	UDP-galactopyranose mutase (UGM) catalyzes the interconversion between UDP-galactopyranose and UDP-galactofuranose. Absent in humans, galactofuranose is found in bacterial and fungal cell walls and is a cell surface virulence factor in protozoan parasites. For these reasons, UGMs are targets for drug discovery. Here, we report a mutagenesis and structural study of the UGMs from Aspergillus fumigatus and Trypanosoma cruzi focused on active site residues that are conserved in eukaryotic UGMs but are absent or different in bacterial UGMs. Kinetic analysis of the variants F66A, Y104A, Q107A, N207A, and Y317A (A. fumigatus numbering) show decreases in kcat/KM values of 200-1000-fold for the mutase reaction. In contrast, none of the mutations significantly affect the kinetics of enzyme activation by NADPH. These results indicate that the targeted residues are important for promoting the transition state conformation for UDP-galactofuranose formation. Crystal structures of the A. fumigatus mutant enzymes were determined in the presence and absence of UDP to understand the structural consequences of the mutations. The structures suggest important roles for Asn207 in stabilizing the closed active site, and Tyr317 in positioning of the uridine ring. Phe66 and the corresponding residue in Mycobacterium tuberculosis UGM (His68) play a role as the backstop, stabilizing the galactopyranose group for nucleophilic attack. Together, these results provide insight into the essentiality of the targeted residues for realizing maximal catalytic activity and a proposal for how conformational changes that close the active site are temporally related and coupled together.

-	~~The entry 4wx1 is ON HOLD until Paper Publication~~	+	Contributions of Unique Active Site Residues of Eukaryotic UDP-Galactopyranose Mutases to Substrate Recognition and Active Site Dynamics.,Da Fonseca I, Qureshi IA, Mehra-Chaudhary R, Kizjakina K, Tanner JJ, Sobrado P Biochemistry. 2014 Dec 2. PMID:25412209<ref>PMID:25412209</ref>

-	~~Authors: Qureshi~~, I.A.~~, Chaudhary, R~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Structure of Aspergillus fumigatus~~ UDP-~~Galactopyranose~~ mutase ~~mutant F66A determined from a crystal soaked with UDP-Galactopyranose~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: UDP-galactopyranose mutase]]
		+	[[Category: Chaudhary, R]]
		+	[[Category: Qureshi, I A]]
		+	[[Category: Flavin adenine dinucleotide binding]]
		+	[[Category: Isomerase]]
		+	[[Category: Mutase]]
		+	[[Category: Nucleotide binding]]

OCA at 07:11, 26 November 2014

OCA — Wed, 26 Nov 2014 07:11:14 GMT

←Older revision		Revision as of 07:11, 26 November 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4wx1 is ON HOLD	+	The entry 4wx1 is ON HOLD until Paper Publication

	Authors: Qureshi, I.A., Chaudhary, R.		Authors: Qureshi, I.A., Chaudhary, R.

	Description: Structure of Aspergillus fumigatus UDP-Galactopyranose mutase mutant F66A determined from a crystal soaked with UDP-Galactopyranose		Description: Structure of Aspergillus fumigatus UDP-Galactopyranose mutase mutant F66A determined from a crystal soaked with UDP-Galactopyranose

OCA: Protected "4wx1" [edit=sysop:move=sysop]

OCA — Wed, 19 Nov 2014 12:02:00 GMT

Protected "4wx1" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:02, 19 November 2014

OCA: New page: '''Unreleased structure''' The entry 4wx1 is ON HOLD Authors: Qureshi, I.A., Chaudhary, R. Description: Structure of Aspergillus fumigatus UDP-Galactopyranose mutase mutant F66A determ...

OCA — Wed, 19 Nov 2014 12:01:59 GMT

New page: '''Unreleased structure''' The entry 4wx1 is ON HOLD Authors: Qureshi, I.A., Chaudhary, R. Description: Structure of Aspergillus fumigatus UDP-Galactopyranose mutase mutant F66A determ...

New page

'''Unreleased structure'''

The entry 4wx1 is ON HOLD

Authors: Qureshi, I.A., Chaudhary, R.

Description: Structure of Aspergillus fumigatus UDP-Galactopyranose mutase mutant F66A determined from a crystal soaked with UDP-Galactopyranose