4wyh - Revision history

OCA at 09:02, 20 March 2024

OCA — Wed, 20 Mar 2024 09:02:07 GMT

←Older revision		Revision as of 09:02, 20 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4wyh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WYH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WYH FirstGlance]. <br>		<table><tr><td colspan='2'>[[4wyh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WYH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WYH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyh OCA], [https://pdbe.org/4wyh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wyh RCSB], [https://www.ebi.ac.uk/pdbsum/4wyh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wyh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyh OCA], [https://pdbe.org/4wyh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wyh RCSB], [https://www.ebi.ac.uk/pdbsum/4wyh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wyh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q97ZS7_SACS2 Q97ZS7_SACS2]		[https://www.uniprot.org/uniprot/Q97ZS7_SACS2 Q97ZS7_SACS2]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Archaea encode a eukaryotic-type primase comprising a catalytic subunit (PriS) and a noncatalytic subunit (PriL). Here we report the identification of a primase noncatalytic subunit, denoted PriX, from the hyperthermophilic archaeon Sulfolobus solfataricus. Like PriL, PriX is essential for the survival of the organism. The crystallographic analysis complemented by sensitive sequence comparisons shows that PriX is a diverged homologue of the C-terminal domain of PriL but lacks the iron-sulfur cluster. Phylogenomic analysis provides clues on the origin and evolution of PriX. PriX, PriL and PriS form a stable heterotrimer (PriSLX). Both PriSX and PriSLX show far greater affinity for nucleotide substrates and are substantially more active in primer synthesis than the PriSL heterodimer. In addition, PriL, but not PriX, facilitates primer extension by PriS. We propose that the catalytic activity of PriS is modulated through concerted interactions with the two noncatalytic subunits in primer synthesis.
-
-	A primase subunit essential for efficient primer synthesis by an archaeal eukaryotic-type primase.,Liu B, Ouyang S, Makarova KS, Xia Q, Zhu Y, Li Z, Guo L, Koonin EV, Liu ZJ, Huang L Nat Commun. 2015 Jun 22;6:7300. doi: 10.1038/ncomms8300. PMID:26095544<ref>PMID:26095544</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4wyh" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 21:03, 12 April 2023

OCA — Wed, 12 Apr 2023 21:03:36 GMT

←Older revision		Revision as of 21:03, 12 April 2023
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	<StructureSection load='4wyh' size='340' side='right'caption='[[4wyh]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='4wyh' size='340' side='right'caption='[[4wyh]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4wyh]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Sacs2 Sacs2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WYH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4WYH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4wyh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WYH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WYH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSO0502 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SACS2])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyh OCA], [https://pdbe.org/4wyh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wyh RCSB], [https://www.ebi.ac.uk/pdbsum/4wyh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wyh ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4wyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyh OCA], [~~http~~://pdbe.org/4wyh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4wyh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4wyh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4wyh ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q97ZS7_SACS2 Q97ZS7_SACS2]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Sacs2~~]]	+	[[Category: Saccharolobus solfataricus P2]]
-	[[Category: Ouyang, S]]	+	[[Category: Ouyang S]]
-	~~[[Category: A novel noncatalytic subunit archaeal eukaryal-type primase]]~~	+
-	~~[[Category: Replication~~]]	+

OCA at 08:07, 23 May 2019

OCA — Thu, 23 May 2019 08:07:04 GMT

←Older revision		Revision as of 08:07, 23 May 2019
Line 1:		Line 1:

	==Crystal structure of PriX from the hyperthermophilic archaeon Sulfolobus solfataricus==		==Crystal structure of PriX from the hyperthermophilic archaeon Sulfolobus solfataricus==
-	<StructureSection load='4wyh' size='340' side='right' caption='[[4wyh]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='4wyh' size='340' side='right'caption='[[4wyh]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4wyh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WYH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WYH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4wyh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sacs2 Sacs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WYH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WYH FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSO0502 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SACS2])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyh OCA], [http://pdbe.org/4wyh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wyh RCSB], [http://www.ebi.ac.uk/pdbsum/4wyh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wyh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyh OCA], [http://pdbe.org/4wyh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wyh RCSB], [http://www.ebi.ac.uk/pdbsum/4wyh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wyh ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Sacs2]]
	[[Category: Ouyang, S]]		[[Category: Ouyang, S]]
	[[Category: A novel noncatalytic subunit archaeal eukaryal-type primase]]		[[Category: A novel noncatalytic subunit archaeal eukaryal-type primase]]
	[[Category: Replication]]		[[Category: Replication]]

OCA at 12:07, 12 April 2017

OCA — Wed, 12 Apr 2017 12:07:33 GMT

←Older revision		Revision as of 12:07, 12 April 2017
Line 1:		Line 1:
		+
	==Crystal structure of PriX from the hyperthermophilic archaeon Sulfolobus solfataricus==		==Crystal structure of PriX from the hyperthermophilic archaeon Sulfolobus solfataricus==
	<StructureSection load='4wyh' size='340' side='right' caption='[[4wyh]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='4wyh' size='340' side='right' caption='[[4wyh]], [[Resolution\|resolution]] 1.95Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4wyh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WYH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WYH FirstGlance]. <br>		<table><tr><td colspan='2'>[[4wyh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WYH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WYH FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wyh RCSB], [http://www.ebi.ac.uk/pdbsum/4wyh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyh OCA], [http://pdbe.org/4wyh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wyh RCSB], [http://www.ebi.ac.uk/pdbsum/4wyh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wyh ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4wyh" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:13, 1 July 2015

OCA — Wed, 01 Jul 2015 12:13:17 GMT

←Older revision		Revision as of 12:13, 1 July 2015
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wyh RCSB], [http://www.ebi.ac.uk/pdbsum/4wyh PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wyh RCSB], [http://www.ebi.ac.uk/pdbsum/4wyh PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Archaea encode a eukaryotic-type primase comprising a catalytic subunit (PriS) and a noncatalytic subunit (PriL). Here we report the identification of a primase noncatalytic subunit, denoted PriX, from the hyperthermophilic archaeon Sulfolobus solfataricus. Like PriL, PriX is essential for the survival of the organism. The crystallographic analysis complemented by sensitive sequence comparisons shows that PriX is a diverged homologue of the C-terminal domain of PriL but lacks the iron-sulfur cluster. Phylogenomic analysis provides clues on the origin and evolution of PriX. PriX, PriL and PriS form a stable heterotrimer (PriSLX). Both PriSX and PriSLX show far greater affinity for nucleotide substrates and are substantially more active in primer synthesis than the PriSL heterodimer. In addition, PriL, but not PriX, facilitates primer extension by PriS. We propose that the catalytic activity of PriS is modulated through concerted interactions with the two noncatalytic subunits in primer synthesis.
		+
		+	A primase subunit essential for efficient primer synthesis by an archaeal eukaryotic-type primase.,Liu B, Ouyang S, Makarova KS, Xia Q, Zhu Y, Li Z, Guo L, Koonin EV, Liu ZJ, Huang L Nat Commun. 2015 Jun 22;6:7300. doi: 10.1038/ncomms8300. PMID:26095544<ref>PMID:26095544</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:02, 24 June 2015

OCA — Wed, 24 Jun 2015 13:02:14 GMT

←Older revision		Revision as of 13:02, 24 June 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of PriX from the hyperthermophilic archaeon Sulfolobus solfataricus==
-		+	<StructureSection load='4wyh' size='340' side='right' caption='[[4wyh]], [[Resolution\|resolution]] 1.95Å' scene=''>
-	~~The entry~~ 4wyh is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4wyh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WYH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WYH FirstGlance]. <br>
-	~~Authors~~: ~~Ouyang, S~~.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
-		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wyh RCSB], [http://www.ebi.ac.uk/pdbsum/4wyh PDBsum]</span></td></tr>
-	~~Description~~: ~~A protein crystal structure from Sulfolobus solfataricus~~	+	</table>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	__TOC__
		+	</StructureSection>
	[[Category: Ouyang, S]]		[[Category: Ouyang, S]]
		+	[[Category: A novel noncatalytic subunit archaeal eukaryal-type primase]]
		+	[[Category: Replication]]

OCA at 10:08, 10 June 2015

OCA — Wed, 10 Jun 2015 10:08:13 GMT

←Older revision		Revision as of 10:08, 10 June 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4wyh is ON HOLD	+	The entry 4wyh is ON HOLD until Paper Publication

	Authors: Ouyang, S.		Authors: Ouyang, S.

OCA at 09:50, 6 May 2015

OCA — Wed, 06 May 2015 09:50:06 GMT

←Older revision		Revision as of 09:50, 6 May 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4wyh is ON HOLD ~~until Paper Publication~~	+	The entry 4wyh is ON HOLD

	Authors: Ouyang, S.		Authors: Ouyang, S.

OCA at 09:48, 15 April 2015

OCA — Wed, 15 Apr 2015 09:48:01 GMT

←Older revision		Revision as of 09:48, 15 April 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4wyh is ON HOLD	+	The entry 4wyh is ON HOLD until Paper Publication

	Authors: Ouyang, S.		Authors: Ouyang, S.

OCA at 20:12, 24 December 2014

OCA — Wed, 24 Dec 2014 20:12:28 GMT

←Older revision		Revision as of 20:12, 24 December 2014
Line 6:		Line 6:

	Description: A protein crystal structure from Sulfolobus solfataricus		Description: A protein crystal structure from Sulfolobus solfataricus
		+	[[Category: Unreleased Structures]]
		+	[[Category: Ouyang, S]]