4x19 - Revision history

OCA at 10:46, 10 January 2024

OCA — Wed, 10 Jan 2024 10:46:00 GMT

←Older revision		Revision as of 10:46, 10 January 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4x19]] is a 30 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X19 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X19 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4x19]] is a 30 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X19 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X19 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.944Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x19 OCA], [https://pdbe.org/4x19 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x19 RCSB], [https://www.ebi.ac.uk/pdbsum/4x19 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x19 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x19 OCA], [https://pdbe.org/4x19 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x19 RCSB], [https://www.ebi.ac.uk/pdbsum/4x19 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x19 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 21:06, 12 April 2023

OCA — Wed, 12 Apr 2023 21:06:33 GMT

←Older revision		Revision as of 21:06, 12 April 2023
Line 3:		Line 3:
	<StructureSection load='4x19' size='340' side='right'caption='[[4x19]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='4x19' size='340' side='right'caption='[[4x19]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4x19]] is a 30 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X19 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4X19 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4x19]] is a 30 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X19 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X19 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">xylH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x19 OCA], [https://pdbe.org/4x19 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x19 RCSB], [https://www.ebi.ac.uk/pdbsum/4x19 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x19 ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-hydroxymuconate_tautomerase 2-hydroxymuconate tautomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.2.6 5.3.2.6] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4x19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x19 OCA], [~~http~~://pdbe.org/4x19 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4x19 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4x19 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4x19 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/4OT1_PSEPU 4OT1_PSEPU]] Catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate.<ref>PMID:1339435</ref>	+	[https://www.uniprot.org/uniprot/4OT1_PSEPU 4OT1_PSEPU] Catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate.<ref>PMID:1339435</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus fluorescens putidus flugge 1886]]
-	[[Category: 2-hydroxymuconate tautomerase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Poddar, H~~]]	+	[[Category: Pseudomonas putida]]
-	[[Category: ~~Thunnissen, A M.W~~ H]]	+	[[Category: Poddar H]]
-	[[Category: ~~4-oxalocrotonate tautomerase]]~~	+	[[Category: Thunnissen AMWH]]
-	~~[[Category: Beta-alpha-beta structural motif]]~~	+
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Tautomerase superfamily~~]]	+

OCA at 08:41, 23 May 2019

OCA — Thu, 23 May 2019 08:41:11 GMT

←Older revision		Revision as of 08:41, 23 May 2019
Line 1:		Line 1:

	==Crystal structure of native 4-OT from Pseudomonas putida mt-2 at 1.94 Angstrom==		==Crystal structure of native 4-OT from Pseudomonas putida mt-2 at 1.94 Angstrom==
-	<StructureSection load='4x19' size='340' side='right' caption='[[4x19]], [[Resolution\|resolution]] 1.94Å' scene=''>	+	<StructureSection load='4x19' size='340' side='right'caption='[[4x19]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4x19]] is a 30 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X19 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X19 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4x19]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X19 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X19 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">xylH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-hydroxymuconate_tautomerase 2-hydroxymuconate tautomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.2.6 5.3.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-hydroxymuconate_tautomerase 2-hydroxymuconate tautomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.2.6 5.3.2.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x19 OCA], [http://pdbe.org/4x19 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x19 RCSB], [http://www.ebi.ac.uk/pdbsum/4x19 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4x19 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x19 OCA], [http://pdbe.org/4x19 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x19 RCSB], [http://www.ebi.ac.uk/pdbsum/4x19 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4x19 ProSAT]</span></td></tr>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus fluorescens putidus flugge 1886]]
	[[Category: 2-hydroxymuconate tautomerase]]		[[Category: 2-hydroxymuconate tautomerase]]
		+	[[Category: Large Structures]]
	[[Category: Poddar, H]]		[[Category: Poddar, H]]
	[[Category: Thunnissen, A M.W H]]		[[Category: Thunnissen, A M.W H]]

OCA at 00:11, 26 January 2017

OCA — Thu, 26 Jan 2017 00:11:15 GMT

←Older revision		Revision as of 00:11, 26 January 2017
Line 1:		Line 1:
		+
	==Crystal structure of native 4-OT from Pseudomonas putida mt-2 at 1.94 Angstrom==		==Crystal structure of native 4-OT from Pseudomonas putida mt-2 at 1.94 Angstrom==
	<StructureSection load='4x19' size='340' side='right' caption='[[4x19]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='4x19' size='340' side='right' caption='[[4x19]], [[Resolution\|resolution]] 1.94Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-hydroxymuconate_tautomerase 2-hydroxymuconate tautomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.2.6 5.3.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-hydroxymuconate_tautomerase 2-hydroxymuconate tautomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.2.6 5.3.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x19 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x19 RCSB], [http://www.ebi.ac.uk/pdbsum/4x19 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x19 OCA], [http://pdbe.org/4x19 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x19 RCSB], [http://www.ebi.ac.uk/pdbsum/4x19 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4x19 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4x19" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:46, 11 March 2015

OCA — Wed, 11 Mar 2015 11:46:14 GMT

←Older revision		Revision as of 11:46, 11 March 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of native 4-OT from Pseudomonas putida mt-2 at 1.94 Angstrom==
		+	<StructureSection load='4x19' size='340' side='right' caption='[[4x19]], [[Resolution\|resolution]] 1.94Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4x19]] is a 30 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X19 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X19 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-hydroxymuconate_tautomerase 2-hydroxymuconate tautomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.2.6 5.3.2.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x19 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x19 RCSB], [http://www.ebi.ac.uk/pdbsum/4x19 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/4OT1_PSEPU 4OT1_PSEPU]] Catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate.<ref>PMID:1339435</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The enzyme 4-oxalocrotonate tautomerase (4-OT), which has a catalytic N-terminal proline residue (Pro1), can promiscuously catalyze various carbon-carbon bond-forming reactions, including aldol condensation of acetaldehyde with benzaldehyde to yield cinnamaldehyde, and Michael-type addition of acetaldehyde to a wide variety of nitroalkenes to yield valuable gamma-nitroaldehydes. To gain insight into how 4-OT catalyzes these unnatural reactions, we carried out exchange studies in D2 O, and X-ray crystallography studies. The former established that H-D exchange within acetaldehyde is catalyzed by 4-OT and that the Pro1 residue is crucial for this activity. The latter showed that Pro1 of 4-OT had reacted with acetaldehyde to give an enamine species. These results provide evidence of the mechanism of the 4-OT-catalyzed aldol and Michael-type addition reactions in which acetaldehyde is activated for nucleophilic addition by Pro1-dependent formation of an enamine intermediate.

-	~~The entry 4x19 is ON HOLD until Paper Publication~~	+	Evidence for the Formation of an Enamine Species during Aldol and Michael-type Addition Reactions Promiscuously Catalyzed by 4-Oxalocrotonate Tautomerase.,Poddar H, Rahimi M, Geertsema EM, Thunnissen AM, Poelarends GJ Chembiochem. 2015 Feb 26. doi: 10.1002/cbic.201402687. PMID:25728471<ref>PMID:25728471</ref>

-	~~Authors: Thunnissen~~, A.M.~~W.H., Poddar, H~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Crystal structure of native 4-OT from Pseudomonas putida mt-2 at 1.94 Angstrom~~	+	== References ==
-	~~[[Category: Unreleased Structures]]~~	+	<references/>
-	[[Category: ~~Thunnissen, A.M.W.H~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: 2-hydroxymuconate tautomerase]]
	[[Category: Poddar, H]]		[[Category: Poddar, H]]
		+	[[Category: Thunnissen, A M.W H]]
		+	[[Category: 4-oxalocrotonate tautomerase]]
		+	[[Category: Beta-alpha-beta structural motif]]
		+	[[Category: Isomerase]]
		+	[[Category: Tautomerase superfamily]]

OCA at 20:19, 24 December 2014

OCA — Wed, 24 Dec 2014 20:19:19 GMT

←Older revision		Revision as of 20:19, 24 December 2014
Line 6:		Line 6:

	Description: Crystal structure of native 4-OT from Pseudomonas putida mt-2 at 1.94 Angstrom		Description: Crystal structure of native 4-OT from Pseudomonas putida mt-2 at 1.94 Angstrom
		+	[[Category: Unreleased Structures]]
		+	[[Category: Thunnissen, A.M.W.H]]
		+	[[Category: Poddar, H]]

OCA: Protected "4x19" [edit=sysop:move=sysop]

OCA — Wed, 03 Dec 2014 08:45:06 GMT

Protected "4x19" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:45, 3 December 2014

OCA: New page: '''Unreleased structure''' The entry 4x19 is ON HOLD until Paper Publication Authors: Thunnissen, A.M.W.H., Poddar, H. Description: Crystal structure of native 4-OT from Pseudomonas pu...

OCA — Wed, 03 Dec 2014 08:45:05 GMT

New page: '''Unreleased structure''' The entry 4x19 is ON HOLD until Paper Publication Authors: Thunnissen, A.M.W.H., Poddar, H. Description: Crystal structure of native 4-OT from Pseudomonas pu...

New page

'''Unreleased structure'''

The entry 4x19 is ON HOLD until Paper Publication

Authors: Thunnissen, A.M.W.H., Poddar, H.

Description: Crystal structure of native 4-OT from Pseudomonas putida mt-2 at 1.94 Angstrom