4x81 - Revision history

OCA at 13:00, 1 March 2024

OCA — Fri, 01 Mar 2024 13:00:11 GMT

←Older revision		Revision as of 13:00, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4x81]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_griseorubida Micromonospora griseorubida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X81 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4x81]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_griseorubida Micromonospora griseorubida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X81 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MVI:MYCINAMICIN+VI'>MVI</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.59Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MVI:MYCINAMICIN+VI'>MVI</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x81 OCA], [https://pdbe.org/4x81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x81 RCSB], [https://www.ebi.ac.uk/pdbsum/4x81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x81 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x81 OCA], [https://pdbe.org/4x81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x81 RCSB], [https://www.ebi.ac.uk/pdbsum/4x81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x81 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/MYCF_MICGR MYCF_MICGR] O-methyltransferase that catalyzes the conversion of mycinamicin III to mycinamicin IV in the biosynthesis of mycinamicin, a 16-membered macrolide antibiotic.<ref>PMID:19415708</ref> <ref>PMID:7808395</ref> <ref>PMID:8163162</ref>		[https://www.uniprot.org/uniprot/MYCF_MICGR MYCF_MICGR] O-methyltransferase that catalyzes the conversion of mycinamicin III to mycinamicin IV in the biosynthesis of mycinamicin, a 16-membered macrolide antibiotic.<ref>PMID:19415708</ref> <ref>PMID:7808395</ref> <ref>PMID:8163162</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Sugar moieties in natural products are frequently modified by O-methylation. In the biosynthesis of the macrolide antibiotic mycinamicin, methylation of a 6'-deoxyallose substituent occurs in a stepwise manner first at the 2'- and then the 3'-hydroxyl groups to produce the mycinose moiety in the final product. The timing and placement of the O-methylations impact final stage C-H functionalization reactions mediated by the P450 monooxygenase MycG. The structural basis of pathway ordering and substrate specificity is unknown. A series of crystal structures of MycF, the 3'-O-methyltransferase, including the free enzyme and complexes with S-adenosyl homocysteine (SAH), substrate, product, and unnatural substrates, show that SAM binding induces substantial ordering that creates the binding site for the natural substrate, and a bound metal ion positions the substrate for catalysis. A single amino acid substitution relaxed the 2'-methoxy specificity but retained regiospecificity. The engineered variant produced a new mycinamicin analog, demonstrating the utility of structural information to facilitate bioengineering approaches for the chemoenzymatic synthesis of complex small molecules containing modified sugars. Using the MycF substrate complex and the modeled substrate complex of a 4'-specific homologue, active site residues were identified that correlate with the 3' or 4' specificity of MycF family members and define the protein and substrate features that direct the regiochemistry of methyltransfer. This classification scheme will be useful in the annotation of new secondary metabolite pathways that utilize this family of enzymes.
-
-	Structural Basis of Substrate Specificity and Regiochemistry in the MycF/TylF Family of Sugar O-Methyltransferases.,Bernard SM, Akey DL, Tripathi A, Park SR, Konwerski JR, Anzai Y, Li S, Kato F, Sherman DH, Smith JL ACS Chem Biol. 2015 Feb 26. PMID:25692963<ref>PMID:25692963</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4x81" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 21:16, 12 April 2023

OCA — Wed, 12 Apr 2023 21:16:41 GMT

←Older revision		Revision as of 21:16, 12 April 2023
Line 3:		Line 3:
	<StructureSection load='4x81' size='340' side='right'caption='[[4x81]], [[Resolution\|resolution]] 1.59Å' scene=''>		<StructureSection load='4x81' size='340' side='right'caption='[[4x81]], [[Resolution\|resolution]] 1.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4x81]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~'micromonospora_griseorubida' 'micromonospora~~ griseorubida']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X81 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4X81 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4x81]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_griseorubida Micromonospora griseorubida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X81 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MVI:MYCINAMICIN+VI'>MVI</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MVI:MYCINAMICIN+VI'>MVI</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4x7v\|4x7v]], [[4x7z\|4x7z]], [[4x7y\|4x7y]], [[4x7w\|4x7w]], [[4x7x\|4x7x]], [[4x7u\|4x7u]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x81 OCA], [https://pdbe.org/4x81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x81 RCSB], [https://www.ebi.ac.uk/pdbsum/4x81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x81 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mycF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28040 'Micromonospora griseorubida'])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mycinamicin_III_3''-O-methyltransferase Mycinamicin III 3''-O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.237 2.1.1.237] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4x81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x81 OCA], [~~http~~://pdbe.org/4x81 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4x81 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4x81 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4x81 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MYCF_MICGR MYCF_MICGR]] O-methyltransferase that catalyzes the conversion of mycinamicin III to mycinamicin IV in the biosynthesis of mycinamicin, a 16-membered macrolide antibiotic.<ref>PMID:19415708</ref> <ref>PMID:7808395</ref> <ref>PMID:8163162</ref>	+	[https://www.uniprot.org/uniprot/MYCF_MICGR MYCF_MICGR] O-methyltransferase that catalyzes the conversion of mycinamicin III to mycinamicin IV in the biosynthesis of mycinamicin, a 16-membered macrolide antibiotic.<ref>PMID:19415708</ref> <ref>PMID:7808395</ref> <ref>PMID:8163162</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Micromonospora griseorubida]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Mycinamicin III 3''-O-methyltransferase~~]]	+	[[Category: Micromonospora griseorubida]]
-	[[Category: Bernard~~, S M~~]]	+	[[Category: Bernard SM]]
-	[[Category: Smith~~, J L]]~~	+	[[Category: Smith JL]]
-	~~[[Category: Antibiotic]]~~	+
-	~~[[Category: Macrolide]]~~	+
-	~~[[Category: Methyltransferase]]~~	+
-	~~[[Category: Natural product]]~~	+
-	~~[[Category: Transferase-antibiotic complex~~]]	+

OCA at 07:21, 29 May 2019

OCA — Wed, 29 May 2019 07:21:02 GMT

←Older revision		Revision as of 07:21, 29 May 2019
Line 1:		Line 1:

	==MycF mycinamicin III 3'-O-methyltransferase (E35Q, M56A, E139A variant) in complex with Mg, SAH and mycinamicin VI (MycE substrate)==		==MycF mycinamicin III 3'-O-methyltransferase (E35Q, M56A, E139A variant) in complex with Mg, SAH and mycinamicin VI (MycE substrate)==
-	<StructureSection load='4x81' size='340' side='right' caption='[[4x81]], [[Resolution\|resolution]] 1.59Å' scene=''>	+	<StructureSection load='4x81' size='340' side='right'caption='[[4x81]], [[Resolution\|resolution]] 1.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4x81]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X81 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X81 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4x81]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/'micromonospora_griseorubida' 'micromonospora griseorubida']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X81 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X81 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MVI:MYCINAMICIN+VI'>MVI</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MVI:MYCINAMICIN+VI'>MVI</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4x7v\|4x7v]], [[4x7z\|4x7z]], [[4x7y\|4x7y]], [[4x7w\|4x7w]], [[4x7x\|4x7x]], [[4x7u\|4x7u]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4x7v\|4x7v]], [[4x7z\|4x7z]], [[4x7y\|4x7y]], [[4x7w\|4x7w]], [[4x7x\|4x7x]], [[4x7u\|4x7u]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mycF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28040 'Micromonospora griseorubida'])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mycinamicin_III_3''-O-methyltransferase Mycinamicin III 3''-O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.237 2.1.1.237] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mycinamicin_III_3''-O-methyltransferase Mycinamicin III 3''-O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.237 2.1.1.237] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x81 OCA], [http://pdbe.org/4x81 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x81 RCSB], [http://www.ebi.ac.uk/pdbsum/4x81 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4x81 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x81 OCA], [http://pdbe.org/4x81 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x81 RCSB], [http://www.ebi.ac.uk/pdbsum/4x81 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4x81 ProSAT]</span></td></tr>
Line 24:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Micromonospora griseorubida]]
		+	[[Category: Large Structures]]
	[[Category: Mycinamicin III 3''-O-methyltransferase]]		[[Category: Mycinamicin III 3''-O-methyltransferase]]
	[[Category: Bernard, S M]]		[[Category: Bernard, S M]]

OCA at 06:24, 26 July 2016

OCA — Tue, 26 Jul 2016 06:24:03 GMT

←Older revision		Revision as of 06:24, 26 July 2016
Line 1:		Line 1:
		+
	==MycF mycinamicin III 3'-O-methyltransferase (E35Q, M56A, E139A variant) in complex with Mg, SAH and mycinamicin VI (MycE substrate)==		==MycF mycinamicin III 3'-O-methyltransferase (E35Q, M56A, E139A variant) in complex with Mg, SAH and mycinamicin VI (MycE substrate)==
	<StructureSection load='4x81' size='340' side='right' caption='[[4x81]], [[Resolution\|resolution]] 1.59Å' scene=''>		<StructureSection load='4x81' size='340' side='right' caption='[[4x81]], [[Resolution\|resolution]] 1.59Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4x7v\|4x7v]], [[4x7z\|4x7z]], [[4x7y\|4x7y]], [[4x7w\|4x7w]], [[4x7x\|4x7x]], [[4x7u\|4x7u]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4x7v\|4x7v]], [[4x7z\|4x7z]], [[4x7y\|4x7y]], [[4x7w\|4x7w]], [[4x7x\|4x7x]], [[4x7u\|4x7u]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mycinamicin_III_3''-O-methyltransferase Mycinamicin III 3''-O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.237 2.1.1.237] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mycinamicin_III_3''-O-methyltransferase Mycinamicin III 3''-O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.237 2.1.1.237] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x81 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x81 RCSB], [http://www.ebi.ac.uk/pdbsum/4x81 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x81 OCA], [http://pdbe.org/4x81 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x81 RCSB], [http://www.ebi.ac.uk/pdbsum/4x81 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4x81 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4x81" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:22, 4 March 2015

OCA — Wed, 04 Mar 2015 11:22:44 GMT

←Older revision		Revision as of 11:22, 4 March 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==MycF mycinamicin III 3'-O-methyltransferase (E35Q, M56A, E139A variant) in complex with Mg, SAH and mycinamicin VI (MycE substrate)==
		+	<StructureSection load='4x81' size='340' side='right' caption='[[4x81]], [[Resolution\|resolution]] 1.59Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4x81]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X81 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X81 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MVI:MYCINAMICIN+VI'>MVI</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4x7v\|4x7v]], [[4x7z\|4x7z]], [[4x7y\|4x7y]], [[4x7w\|4x7w]], [[4x7x\|4x7x]], [[4x7u\|4x7u]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mycinamicin_III_3''-O-methyltransferase Mycinamicin III 3''-O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.237 2.1.1.237] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x81 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x81 RCSB], [http://www.ebi.ac.uk/pdbsum/4x81 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MYCF_MICGR MYCF_MICGR]] O-methyltransferase that catalyzes the conversion of mycinamicin III to mycinamicin IV in the biosynthesis of mycinamicin, a 16-membered macrolide antibiotic.<ref>PMID:19415708</ref> <ref>PMID:7808395</ref> <ref>PMID:8163162</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sugar moieties in natural products are frequently modified by O-methylation. In the biosynthesis of the macrolide antibiotic mycinamicin, methylation of a 6'-deoxyallose substituent occurs in a stepwise manner first at the 2'- and then the 3'-hydroxyl groups to produce the mycinose moiety in the final product. The timing and placement of the O-methylations impact final stage C-H functionalization reactions mediated by the P450 monooxygenase MycG. The structural basis of pathway ordering and substrate specificity is unknown. A series of crystal structures of MycF, the 3'-O-methyltransferase, including the free enzyme and complexes with S-adenosyl homocysteine (SAH), substrate, product, and unnatural substrates, show that SAM binding induces substantial ordering that creates the binding site for the natural substrate, and a bound metal ion positions the substrate for catalysis. A single amino acid substitution relaxed the 2'-methoxy specificity but retained regiospecificity. The engineered variant produced a new mycinamicin analog, demonstrating the utility of structural information to facilitate bioengineering approaches for the chemoenzymatic synthesis of complex small molecules containing modified sugars. Using the MycF substrate complex and the modeled substrate complex of a 4'-specific homologue, active site residues were identified that correlate with the 3' or 4' specificity of MycF family members and define the protein and substrate features that direct the regiochemistry of methyltransfer. This classification scheme will be useful in the annotation of new secondary metabolite pathways that utilize this family of enzymes.

-	~~The entry 4x81 is ON HOLD until Paper Publication~~	+	Structural Basis of Substrate Specificity and Regiochemistry in the MycF/TylF Family of Sugar O-Methyltransferases.,Bernard SM, Akey DL, Tripathi A, Park SR, Konwerski JR, Anzai Y, Li S, Kato F, Sherman DH, Smith JL ACS Chem Biol. 2015 Feb 26. PMID:25692963<ref>PMID:25692963</ref>

-	~~Authors: Bernard~~, S.M., Smith, J.L.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~:	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: ~~Smith, J.L~~]]	+	__TOC__
-	[[Category: ~~Bernard, S.M~~]]	+	</StructureSection>
		+	[[Category: Mycinamicin III 3''-O-methyltransferase]]
		+	[[Category: Bernard, S M]]
		+	[[Category: Smith, J L]]
		+	[[Category: Antibiotic]]
		+	[[Category: Macrolide]]
		+	[[Category: Methyltransferase]]
		+	[[Category: Natural product]]
		+	[[Category: Transferase-antibiotic complex]]

OCA at 15:11, 28 January 2015

OCA — Wed, 28 Jan 2015 15:11:03 GMT

←Older revision		Revision as of 15:11, 28 January 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4x81 is ON HOLD	+	The entry 4x81 is ON HOLD until Paper Publication

	Authors: Bernard, S.M., Smith, J.L.		Authors: Bernard, S.M., Smith, J.L.

OCA at 20:37, 24 December 2014

OCA — Wed, 24 Dec 2014 20:37:52 GMT

←Older revision		Revision as of 20:37, 24 December 2014
Line 5:		Line 5:
	Authors: Bernard, S.M., Smith, J.L.		Authors: Bernard, S.M., Smith, J.L.

-	Description:	+	Description:
		+	[[Category: Unreleased Structures]]
		+	[[Category: Smith, J.L]]
		+	[[Category: Bernard, S.M]]

OCA: Protected "4x81" [edit=sysop:move=sysop]

OCA — Wed, 17 Dec 2014 11:46:25 GMT

Protected "4x81" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:46, 17 December 2014

OCA: New page: '''Unreleased structure''' The entry 4x81 is ON HOLD Authors: Bernard, S.M., Smith, J.L. Description:

OCA — Wed, 17 Dec 2014 11:46:23 GMT

New page: '''Unreleased structure''' The entry 4x81 is ON HOLD Authors: Bernard, S.M., Smith, J.L. Description:

New page

'''Unreleased structure'''

The entry 4x81 is ON HOLD

Authors: Bernard, S.M., Smith, J.L.

Description: