4xav - Revision history

OCA at 13:00, 1 March 2024

OCA — Fri, 01 Mar 2024 13:00:22 GMT

←Older revision		Revision as of 13:00, 1 March 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/GLDN_MOUSE GLDN_MOUSE] Plays a role in the formation of the nodes of Ranvier along myelinated axons. Probable NRCAM and NFASC/neurofascin ligand which may provide a glial positional clue required for the proper molecular assembly of the nodes of Ranvier (By similarity).		[https://www.uniprot.org/uniprot/GLDN_MOUSE GLDN_MOUSE] Plays a role in the formation of the nodes of Ranvier along myelinated axons. Probable NRCAM and NFASC/neurofascin ligand which may provide a glial positional clue required for the proper molecular assembly of the nodes of Ranvier (By similarity).
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Olfactomedin (OLF) domains are found within extracellular, multidomain proteins in numerous tissues of multicellular organisms. Even though these proteins have been implicated in human disorders ranging from cancers to attention deficit disorder to glaucoma, little is known about their structure(s) and function(s). Here we biophysically, biochemically, and structurally characterize OLF domains from H. sapiens olfactomedin-1 (npoh-OLF, also called noelin, pancortin, OLFM1, and hOlfA), and M. musculus gliomedin (glio-OLF, also called collomin, collmin, and CRG-L2), and compare them with available structures of myocilin (myoc-OLF) recently reported by us and R. norvegicus glio-OLF and M. musculus latrophilin-3 (lat3-OLF) by others. Although the five-bladed beta-propeller architecture remains unchanged, numerous physicochemical characteristics differ among these OLF domains. First, npoh-OLF and glio-OLF exhibit prominent, yet distinct, positive surface charges and copurify with polynucleotides. Second, whereas npoh-OLF and myoc-OLF exhibit thermal stabilities typical of human proteins near 55 degrees C, and most myoc-OLF variants are destabilized and highly prone to aggregation, glio-OLF is nearly 20 degrees C more stable and significantly more resistant to chemical denaturation. Phylogenetically, glio-OLF is most similar to primitive OLFs, and structurally, glio-OLF is missing distinguishing features seen in OLFs such as the disulfide bond formed by N- and C- terminal cysteines, the sequestered Ca2+ ion within the propeller central hydrophilic cavity, and a key loop-stabilizing cation-pi interaction on the top face of npoh-OLF and myoc-OLF. While deciphering the explicit biological functions, ligands, and binding partners for OLF domains will likely continue to be a challenging long-term experimental pursuit, we used structural insights gained here to generate a new antibody selective for myoc-OLF over npoh-OLF and glio-OLF as a first step in overcoming the impasse in detailed functional characterization of these biomedically important protein domains.
-
-	Molecular Details of Olfactomedin Domains Provide Pathway to Structure-Function Studies.,Hill SE, Donegan RK, Nguyen E, Desai TM, Lieberman RL PLoS One. 2015 Jun 29;10(6):e0130888. doi: 10.1371/journal.pone.0130888., eCollection 2015. PMID:26121352<ref>PMID:26121352</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4xav" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:19, 3 January 2024

OCA — Wed, 03 Jan 2024 11:19:53 GMT

←Older revision		Revision as of 11:19, 3 January 2024
Line 3:		Line 3:
	<StructureSection load='4xav' size='340' side='right'caption='[[4xav]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='4xav' size='340' side='right'caption='[[4xav]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4xav]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XAV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XAV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4xav]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XAV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XAV FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.052Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[4xat\|4xat]]~~</~~div~~><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">Gldn, Crgl2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xav OCA], [https://pdbe.org/4xav PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xav RCSB], [https://www.ebi.ac.uk/pdbsum/4xav PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xav ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xav OCA], [https://pdbe.org/4xav PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xav RCSB], [https://www.ebi.ac.uk/pdbsum/4xav PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xav ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GLDN_MOUSE GLDN_MOUSE]] Plays a role in the formation of the nodes of Ranvier along myelinated axons. Probable NRCAM and NFASC/neurofascin ligand which may provide a glial positional clue required for the proper molecular assembly of the nodes of Ranvier (By similarity).	+	[https://www.uniprot.org/uniprot/GLDN_MOUSE GLDN_MOUSE] Plays a role in the formation of the nodes of Ranvier along myelinated axons. Probable NRCAM and NFASC/neurofascin ligand which may provide a glial positional clue required for the proper molecular assembly of the nodes of Ranvier (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Hill~~, S E~~]]	+	[[Category: Hill SE]]
-	[[Category: Lieberman~~, R L~~]]	+	[[Category: Lieberman RL]]
-	[[Category: Nguyen, E]]	+	[[Category: Nguyen E]]
-	~~[[Category: Bladed propeller]]~~	+
-	~~[[Category: Beta propeller]]~~	+
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Olfactomedin~~]]	+

OCA at 10:52, 30 March 2022

OCA — Wed, 30 Mar 2022 10:52:17 GMT

←Older revision		Revision as of 10:52, 30 March 2022
Line 1:		Line 1:

	==Crystal structure of olfactomedin domain from gliomedin==		==Crystal structure of olfactomedin domain from gliomedin==
-	<StructureSection load='4xav' size='340' side='right' caption='[[4xav]], [[Resolution\|resolution]] 2.05Å' scene=''>	+	<StructureSection load='4xav' size='340' side='right'caption='[[4xav]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4xav]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XAV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4XAV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4xav]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XAV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XAV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xat\|4xat]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4xat\|4xat]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gldn, Crgl2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gldn, Crgl2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4xav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xav OCA], [~~http~~://pdbe.org/4xav PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4xav RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4xav PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4xav ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xav OCA], [https://pdbe.org/4xav PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xav RCSB], [https://www.ebi.ac.uk/pdbsum/4xav PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xav ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GLDN_MOUSE GLDN_MOUSE]] Plays a role in the formation of the nodes of Ranvier along myelinated axons. Probable NRCAM and NFASC/neurofascin ligand which may provide a glial positional clue required for the proper molecular assembly of the nodes of Ranvier (By similarity).	+	[[https://www.uniprot.org/uniprot/GLDN_MOUSE GLDN_MOUSE]] Plays a role in the formation of the nodes of Ranvier along myelinated axons. Probable NRCAM and NFASC/neurofascin ligand which may provide a glial positional clue required for the proper molecular assembly of the nodes of Ranvier (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Hill, S E]]		[[Category: Hill, S E]]

OCA at 09:33, 22 November 2017

OCA — Wed, 22 Nov 2017 09:33:27 GMT

←Older revision		Revision as of 09:33, 22 November 2017
Line 3:		Line 3:
	<StructureSection load='4xav' size='340' side='right' caption='[[4xav]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='4xav' size='340' side='right' caption='[[4xav]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4xav]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XAV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XAV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4xav]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XAV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XAV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xat\|4xat]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xat\|4xat]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gldn, Crgl2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xav OCA], [http://pdbe.org/4xav PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xav RCSB], [http://www.ebi.ac.uk/pdbsum/4xav PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xav ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xav OCA], [http://pdbe.org/4xav PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xav RCSB], [http://www.ebi.ac.uk/pdbsum/4xav PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xav ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Hill, S E]]		[[Category: Hill, S E]]
	[[Category: Lieberman, R L]]		[[Category: Lieberman, R L]]

OCA at 13:27, 19 April 2017

OCA — Wed, 19 Apr 2017 13:27:00 GMT

←Older revision		Revision as of 13:27, 19 April 2017
Line 1:		Line 1:
		+
	==Crystal structure of olfactomedin domain from gliomedin==		==Crystal structure of olfactomedin domain from gliomedin==
	<StructureSection load='4xav' size='340' side='right' caption='[[4xav]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='4xav' size='340' side='right' caption='[[4xav]], [[Resolution\|resolution]] 2.05Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xat\|4xat]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xat\|4xat]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xav OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xav RCSB], [http://www.ebi.ac.uk/pdbsum/4xav PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xav OCA], [http://pdbe.org/4xav PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xav RCSB], [http://www.ebi.ac.uk/pdbsum/4xav PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xav ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4xav" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:15, 15 July 2015

OCA — Wed, 15 Jul 2015 13:15:38 GMT

←Older revision		Revision as of 13:15, 15 July 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of olfactomedin domain from gliomedin==
		+	<StructureSection load='4xav' size='340' side='right' caption='[[4xav]], [[Resolution\|resolution]] 2.05Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4xav]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XAV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XAV FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xat\|4xat]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xav OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xav RCSB], [http://www.ebi.ac.uk/pdbsum/4xav PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GLDN_MOUSE GLDN_MOUSE]] Plays a role in the formation of the nodes of Ranvier along myelinated axons. Probable NRCAM and NFASC/neurofascin ligand which may provide a glial positional clue required for the proper molecular assembly of the nodes of Ranvier (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Olfactomedin (OLF) domains are found within extracellular, multidomain proteins in numerous tissues of multicellular organisms. Even though these proteins have been implicated in human disorders ranging from cancers to attention deficit disorder to glaucoma, little is known about their structure(s) and function(s). Here we biophysically, biochemically, and structurally characterize OLF domains from H. sapiens olfactomedin-1 (npoh-OLF, also called noelin, pancortin, OLFM1, and hOlfA), and M. musculus gliomedin (glio-OLF, also called collomin, collmin, and CRG-L2), and compare them with available structures of myocilin (myoc-OLF) recently reported by us and R. norvegicus glio-OLF and M. musculus latrophilin-3 (lat3-OLF) by others. Although the five-bladed beta-propeller architecture remains unchanged, numerous physicochemical characteristics differ among these OLF domains. First, npoh-OLF and glio-OLF exhibit prominent, yet distinct, positive surface charges and copurify with polynucleotides. Second, whereas npoh-OLF and myoc-OLF exhibit thermal stabilities typical of human proteins near 55 degrees C, and most myoc-OLF variants are destabilized and highly prone to aggregation, glio-OLF is nearly 20 degrees C more stable and significantly more resistant to chemical denaturation. Phylogenetically, glio-OLF is most similar to primitive OLFs, and structurally, glio-OLF is missing distinguishing features seen in OLFs such as the disulfide bond formed by N- and C- terminal cysteines, the sequestered Ca2+ ion within the propeller central hydrophilic cavity, and a key loop-stabilizing cation-pi interaction on the top face of npoh-OLF and myoc-OLF. While deciphering the explicit biological functions, ligands, and binding partners for OLF domains will likely continue to be a challenging long-term experimental pursuit, we used structural insights gained here to generate a new antibody selective for myoc-OLF over npoh-OLF and glio-OLF as a first step in overcoming the impasse in detailed functional characterization of these biomedically important protein domains.

-	~~The entry 4xav is ON HOLD until Paper Publication~~	+	Molecular Details of Olfactomedin Domains Provide Pathway to Structure-Function Studies.,Hill SE, Donegan RK, Nguyen E, Desai TM, Lieberman RL PLoS One. 2015 Jun 29;10(6):e0130888. doi: 10.1371/journal.pone.0130888., eCollection 2015. PMID:26121352<ref>PMID:26121352</ref>

-	~~Authors: Hill~~, S.~~E., Nguyen, E., Lieberman, R.L~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of olfactomedin domain from gliomedin~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Hill, S E]]
		+	[[Category: Lieberman, R L]]
	[[Category: Nguyen, E]]		[[Category: Nguyen, E]]
-	[[Category: ~~Lieberman, R.L~~]]	+	[[Category: Bladed propeller]]
-	[[Category: ~~Hill, S.E~~]]	+	[[Category: Beta propeller]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Olfactomedin]]

OCA at 15:41, 21 January 2015

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-	The entry 4xav is ON HOLD	+	The entry 4xav is ON HOLD until Paper Publication

	Authors: Hill, S.E., Nguyen, E., Lieberman, R.L.		Authors: Hill, S.E., Nguyen, E., Lieberman, R.L.

OCA at 20:47, 24 December 2014

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	Description: Crystal structure of olfactomedin domain from gliomedin		Description: Crystal structure of olfactomedin domain from gliomedin
		+	[[Category: Unreleased Structures]]
		+	[[Category: Nguyen, E]]
		+	[[Category: Lieberman, R.L]]
		+	[[Category: Hill, S.E]]

OCA: Protected "4xav" [edit=sysop:move=sysop]

OCA — Wed, 24 Dec 2014 10:04:09 GMT

Protected "4xav" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:04, 24 December 2014

OCA: New page: '''Unreleased structure''' The entry 4xav is ON HOLD Authors: Hill, S.E., Nguyen, E., Lieberman, R.L. Description: Crystal structure of olfactomedin domain from gliomedin

OCA — Wed, 24 Dec 2014 10:04:08 GMT

New page: '''Unreleased structure''' The entry 4xav is ON HOLD Authors: Hill, S.E., Nguyen, E., Lieberman, R.L. Description: Crystal structure of olfactomedin domain from gliomedin

New page

'''Unreleased structure'''

The entry 4xav is ON HOLD

Authors: Hill, S.E., Nguyen, E., Lieberman, R.L.

Description: Crystal structure of olfactomedin domain from gliomedin