4xjn - Revision history

OCA at 17:39, 26 April 2023

OCA — Wed, 26 Apr 2023 17:39:41 GMT

←Older revision		Revision as of 17:39, 26 April 2023
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	<StructureSection load='4xjn' size='340' side='right'caption='[[4xjn]], [[Resolution\|resolution]] 3.11Å' scene=''>		<StructureSection load='4xjn' size='340' side='right'caption='[[4xjn]], [[Resolution\|resolution]] 3.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4xjn]] is a 14 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XJN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4XJN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4xjn]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Mammalian_orthorubulavirus_5 Mammalian orthorubulavirus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XJN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XJN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PB:LEAD+(II)+ION'>PB</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PB:LEAD+(II)+ION'>PB</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4xjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xjn OCA], [~~http~~://pdbe.org/4xjn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4xjn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4xjn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4xjn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xjn OCA], [https://pdbe.org/4xjn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xjn RCSB], [https://www.ebi.ac.uk/pdbsum/4xjn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xjn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~W5QKM4_9PARA W5QKM4_9PARA]~~] Encapsidates the genome, protecting it from nucleases.~~[RuleBase:RU361245]~~	+	[https://www.uniprot.org/uniprot/NCAP_PIV5 NCAP_PIV5] Encapsidates the genome, protecting it from nucleases. The nucleocapsid (NC) has a helical structure. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Alayyoubi, M~~]]	+	[[Category: Mammalian orthorubulavirus 5]]
-	[[Category: ~~Kors, C A~~]]	+	[[Category: Alayyoubi M]]
-	[[Category: ~~Lamb, R A~~]]	+	[[Category: Kors CA]]
-	[[Category: ~~Leser, G P~~]]	+	[[Category: Lamb RA]]
-	[[Category: ~~Complex]]~~	+	[[Category: Leser GP]]
-	~~[[Category: Nucleocapsid]]~~	+
-	~~[[Category: Piv5]]~~	+
-	~~[[Category: Rna]]~~	+
-	~~[[Category: Viral protein-rna complex~~]]	+

OCA at 07:31, 4 March 2020

OCA — Wed, 04 Mar 2020 07:31:03 GMT

←Older revision		Revision as of 07:31, 4 March 2020
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	==Structure of the parainfluenza virus 5 nucleocapsid-RNA complex: an insight into paramyxovirus polymerase activity==		==Structure of the parainfluenza virus 5 nucleocapsid-RNA complex: an insight into paramyxovirus polymerase activity==
-	<StructureSection load='4xjn' size='340' side='right' caption='[[4xjn]], [[Resolution\|resolution]] 3.11Å' scene=''>	+	<StructureSection load='4xjn' size='340' side='right'caption='[[4xjn]], [[Resolution\|resolution]] 3.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4xjn]] is a 14 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XJN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XJN FirstGlance]. <br>		<table><tr><td colspan='2'>[[4xjn]] is a 14 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XJN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XJN FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 4xjn" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4xjn" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Nucleoprotein 3D structures\|Nucleoprotein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Alayyoubi, M]]		[[Category: Alayyoubi, M]]
	[[Category: Kors, C A]]		[[Category: Kors, C A]]

OCA at 22:47, 15 November 2017

OCA — Wed, 15 Nov 2017 22:47:17 GMT

←Older revision		Revision as of 22:47, 15 November 2017
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	[[Category: Piv5]]		[[Category: Piv5]]
	[[Category: Rna]]		[[Category: Rna]]
		+	[[Category: Viral protein-rna complex]]

OCA at 18:34, 1 February 2017

OCA — Wed, 01 Feb 2017 18:34:34 GMT

←Older revision		Revision as of 18:34, 1 February 2017
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		+
	==Structure of the parainfluenza virus 5 nucleocapsid-RNA complex: an insight into paramyxovirus polymerase activity==		==Structure of the parainfluenza virus 5 nucleocapsid-RNA complex: an insight into paramyxovirus polymerase activity==
	<StructureSection load='4xjn' size='340' side='right' caption='[[4xjn]], [[Resolution\|resolution]] 3.11Å' scene=''>		<StructureSection load='4xjn' size='340' side='right' caption='[[4xjn]], [[Resolution\|resolution]] 3.11Å' scene=''>
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	<table><tr><td colspan='2'>[[4xjn]] is a 14 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XJN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XJN FirstGlance]. <br>		<table><tr><td colspan='2'>[[4xjn]] is a 14 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XJN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XJN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PB:LEAD+(II)+ION'>PB</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PB:LEAD+(II)+ION'>PB</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xjn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xjn RCSB], [http://www.ebi.ac.uk/pdbsum/4xjn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xjn OCA], [http://pdbe.org/4xjn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xjn RCSB], [http://www.ebi.ac.uk/pdbsum/4xjn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xjn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4xjn" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:18, 15 April 2015

OCA — Wed, 15 Apr 2015 06:18:52 GMT

←Older revision		Revision as of 06:18, 15 April 2015
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/W5QKM4_9PARA W5QKM4_9PARA]] Encapsidates the genome, protecting it from nucleases.[RuleBase:RU361245]		[[http://www.uniprot.org/uniprot/W5QKM4_9PARA W5QKM4_9PARA]] Encapsidates the genome, protecting it from nucleases.[RuleBase:RU361245]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Parainfluenza virus 5 (PIV5) is a member of the Paramyxoviridae family of membrane-enveloped viruses with a negative-sense RNA genome that is packaged and protected by long filamentous nucleocapsid-helix structures (RNPs). These RNPs, consisting of approximately 2,600 protomers of nucleocapsid (N) protein, form the template for viral transcription and replication. We have determined the 3D X-ray crystal structure of the nucleoprotein (N)-RNA complex from PIV5 to 3.11-A resolution. The structure reveals a 13-mer nucleocapsid ring whose diameter, cavity, and pitch/height dimensions agree with EM data from early studies on the Paramyxovirinae subfamily of native RNPs, indicating that it closely represents one-turn in the building block of the RNP helices. The PIV5-N nucleocapsid ring encapsidates a nuclease resistant 78-nt RNA strand in its positively charged groove formed between the N-terminal (NTD) and C-terminal (CTD) domains of its successive N protomers. Six nucleotides precisely are associated with each N protomer, with alternating three-base-in three-base-out conformation. The binding of six nucleotides per protomer is consistent with the "rule of six" that governs the genome packaging of the Paramyxovirinae subfamily of viruses. PIV5-N protomer subdomains are very similar in structure to the previously solved Nipah-N structure, but with a difference in the angle between NTD/CTD at the RNA hinge region. Based on the Nipah-N structure we modeled a PIV5-N open conformation in which the CTD rotates away from the RNA strand into the inner spacious nucleocapsid-ring cavity. This rotation would expose the RNA for the viral polymerase activity without major disruption of the nucleocapsid structure.
		+
		+	Structure of the paramyxovirus parainfluenza virus 5 nucleoprotein-RNA complex.,Alayyoubi M, Leser GP, Kors CA, Lamb RA Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):E1792-9. doi:, 10.1073/pnas.1503941112. Epub 2015 Mar 23. PMID:25831513<ref>PMID:25831513</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 12:17, 18 March 2015

OCA — Wed, 18 Mar 2015 12:17:38 GMT

←Older revision		Revision as of 12:17, 18 March 2015
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-	'''~~Unreleased structure~~'''	+	==Structure of the parainfluenza virus 5 nucleocapsid-RNA complex: an insight into paramyxovirus polymerase activity==
-		+	<StructureSection load='4xjn' size='340' side='right' caption='[[4xjn]], [[Resolution\|resolution]] 3.11Å' scene=''>
-	~~The entry~~ 4xjn is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4xjn]] is a 14 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XJN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XJN FirstGlance]. <br>
-	~~Authors~~: ~~Alayyoubi, M~~.~~, Leser, G~~.P.~~, Lamb, R~~.A.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PB:LEAD+(II)+ION'>PB</scene></td></tr>
-		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xjn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xjn RCSB], [http://www.ebi.ac.uk/pdbsum/4xjn PDBsum]</span></td></tr>
-	~~Description~~: ~~Structure of the parainfluenza virus 5 nucleocapsid~~-~~RNA complex~~: ~~an insight into paramyxovirus polymerase activity~~.	+	</table>
-	[~~[Category~~: ~~Unreleased Structures~~]]	+	== Function ==
-	[[~~Category~~: ~~Leser, G~~.P]]	+	[[http://www.uniprot.org/uniprot/W5QKM4_9PARA W5QKM4_9PARA]] Encapsidates the genome, protecting it from nucleases.[RuleBase:RU361245]
		+	__TOC__
		+	</StructureSection>
	[[Category: Alayyoubi, M]]		[[Category: Alayyoubi, M]]
-	[[Category: Lamb, R.A]]	+	[[Category: Kors, C A]]
		+	[[Category: Lamb, R A]]
		+	[[Category: Leser, G P]]
		+	[[Category: Complex]]
		+	[[Category: Nucleocapsid]]
		+	[[Category: Piv5]]
		+	[[Category: Rna]]

OCA at 14:32, 7 February 2015

OCA — Sat, 07 Feb 2015 14:32:30 GMT

←Older revision		Revision as of 14:32, 7 February 2015
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4xjn is ON HOLD	+	The entry 4xjn is ON HOLD until Paper Publication

-	Authors: Alayyoubi, M., Leser, G.P~~., Jardetzky, T.S~~., Lamb, R.A.	+	Authors: Alayyoubi, M., Leser, G.P., Lamb, R.A.

	Description: Structure of the parainfluenza virus 5 nucleocapsid-RNA complex: an insight into paramyxovirus polymerase activity.		Description: Structure of the parainfluenza virus 5 nucleocapsid-RNA complex: an insight into paramyxovirus polymerase activity.
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	[[Category: Alayyoubi, M]]		[[Category: Alayyoubi, M]]
	[[Category: Lamb, R.A]]		[[Category: Lamb, R.A]]
-	[[Category: Jardetzky, T.S]]

OCA: Protected "4xjn" [edit=sysop:move=sysop]

OCA — Wed, 14 Jan 2015 12:48:21 GMT

Protected "4xjn" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:48, 14 January 2015

OCA: New page: '''Unreleased structure''' The entry 4xjn is ON HOLD Authors: Alayyoubi, M., Leser, G.P., Jardetzky, T.S., Lamb, R.A. Description: Structure of the parainfluenza virus 5 nucleocapsid-R...

OCA — Wed, 14 Jan 2015 12:48:20 GMT

New page: '''Unreleased structure''' The entry 4xjn is ON HOLD Authors: Alayyoubi, M., Leser, G.P., Jardetzky, T.S., Lamb, R.A. Description: Structure of the parainfluenza virus 5 nucleocapsid-R...

New page

'''Unreleased structure'''

The entry 4xjn is ON HOLD

Authors: Alayyoubi, M., Leser, G.P., Jardetzky, T.S., Lamb, R.A.

Description: Structure of the parainfluenza virus 5 nucleocapsid-RNA complex: an insight into paramyxovirus polymerase activity.
[[Category: Unreleased Structures]]
[[Category: Leser, G.P]]
[[Category: Alayyoubi, M]]
[[Category: Lamb, R.A]]
[[Category: Jardetzky, T.S]]