4xjv - Revision history

OCA at 07:46, 27 September 2023

OCA — Wed, 27 Sep 2023 07:46:44 GMT

←Older revision		Revision as of 07:46, 27 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4xjv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XJV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XJV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4xjv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XJV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XJV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xjv OCA], [https://pdbe.org/4xjv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xjv RCSB], [https://www.ebi.ac.uk/pdbsum/4xjv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xjv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xjv OCA], [https://pdbe.org/4xjv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xjv RCSB], [https://www.ebi.ac.uk/pdbsum/4xjv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xjv ProSAT]</span></td></tr>
	</table>		</table>

OCA at 17:40, 26 April 2023

OCA — Wed, 26 Apr 2023 17:40:36 GMT

←Older revision		Revision as of 17:40, 26 April 2023
Line 3:		Line 3:
	<StructureSection load='4xjv' size='340' side='right'caption='[[4xjv]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='4xjv' size='340' side='right'caption='[[4xjv]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4xjv]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XJV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4XJV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4xjv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XJV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XJV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">OLAH, THEDC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xjv OCA], [https://pdbe.org/4xjv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xjv RCSB], [https://www.ebi.ac.uk/pdbsum/4xjv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xjv ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oleoyl-[acyl-carrier-protein]_hydrolase Oleoyl-[acyl-carrier-protein] hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.14 3.1.2.14] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xjv OCA], [~~http~~://pdbe.org/4xjv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4xjv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4xjv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4xjv ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SAST_HUMAN SAST_HUMAN]] In fatty acid biosynthesis chain termination and release of the free fatty acid product is achieved by hydrolysis of the thio ester by a thioesterase I, a component of the fatty acid synthetase complex. The chain length of the released fatty acid is usually C16. However, in the mammary glands of non-ruminant mammals, and in the uropygial gland of certain waterfowl there exists a second thioesterase which releases medium-chain length fatty acids (C8 to C2) (By similarity).	+	[https://www.uniprot.org/uniprot/SAST_HUMAN SAST_HUMAN] In fatty acid biosynthesis chain termination and release of the free fatty acid product is achieved by hydrolysis of the thio ester by a thioesterase I, a component of the fatty acid synthetase complex. The chain length of the released fatty acid is usually C16. However, in the mammary glands of non-ruminant mammals, and in the uropygial gland of certain waterfowl there exists a second thioesterase which releases medium-chain length fatty acids (C8 to C2) (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kridel~~, S J~~]]	+	[[Category: Kridel SJ]]
-	[[Category: Lowther~~, W T~~]]	+	[[Category: Lowther WT]]
-	[[Category: Ritchie~~, M K]]~~	+	[[Category: Ritchie MK]]
-	~~[[Category: Closed conformation]]~~	+
-	~~[[Category: Fatty acid synthase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Lipid metabolism~~]]	+

OCA at 08:45, 4 December 2019

OCA — Wed, 04 Dec 2019 08:45:04 GMT

←Older revision		Revision as of 08:45, 4 December 2019
Line 1:		Line 1:

	==Crystal structure of human thioesterase 2==		==Crystal structure of human thioesterase 2==
-	<StructureSection load='4xjv' size='340' side='right' caption='[[4xjv]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='4xjv' size='340' side='right'caption='[[4xjv]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4xjv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XJV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XJV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4xjv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XJV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XJV FirstGlance]. <br>
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Kridel, S J]]		[[Category: Kridel, S J]]
	[[Category: Lowther, W T]]		[[Category: Lowther, W T]]

OCA at 15:30, 15 November 2017

OCA — Wed, 15 Nov 2017 15:30:51 GMT

←Older revision		Revision as of 15:30, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='4xjv' size='340' side='right' caption='[[4xjv]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='4xjv' size='340' side='right' caption='[[4xjv]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4xjv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XJV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XJV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4xjv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XJV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XJV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">OLAH, THEDC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oleoyl-[acyl-carrier-protein]_hydrolase Oleoyl-[acyl-carrier-protein] hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.14 3.1.2.14] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oleoyl-[acyl-carrier-protein]_hydrolase Oleoyl-[acyl-carrier-protein] hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.14 3.1.2.14] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xjv OCA], [http://pdbe.org/4xjv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xjv RCSB], [http://www.ebi.ac.uk/pdbsum/4xjv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xjv OCA], [http://pdbe.org/4xjv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xjv RCSB], [http://www.ebi.ac.uk/pdbsum/4xjv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xjv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Kridel, S J]]		[[Category: Kridel, S J]]
	[[Category: Lowther, W T]]		[[Category: Lowther, W T]]

OCA at 10:13, 26 February 2016

OCA — Fri, 26 Feb 2016 10:13:34 GMT

←Older revision		Revision as of 10:13, 26 February 2016
Line 1:		Line 1:
		+
	==Crystal structure of human thioesterase 2==		==Crystal structure of human thioesterase 2==
	<StructureSection load='4xjv' size='340' side='right' caption='[[4xjv]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='4xjv' size='340' side='right' caption='[[4xjv]], [[Resolution\|resolution]] 2.80Å' scene=''>

OCA at 11:38, 30 December 2015

OCA — Wed, 30 Dec 2015 11:38:41 GMT

←Older revision		Revision as of 11:38, 30 December 2015
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/SAST_HUMAN SAST_HUMAN]] In fatty acid biosynthesis chain termination and release of the free fatty acid product is achieved by hydrolysis of the thio ester by a thioesterase I, a component of the fatty acid synthetase complex. The chain length of the released fatty acid is usually C16. However, in the mammary glands of non-ruminant mammals, and in the uropygial gland of certain waterfowl there exists a second thioesterase which releases medium-chain length fatty acids (C8 to C2) (By similarity).		[[http://www.uniprot.org/uniprot/SAST_HUMAN SAST_HUMAN]] In fatty acid biosynthesis chain termination and release of the free fatty acid product is achieved by hydrolysis of the thio ester by a thioesterase I, a component of the fatty acid synthetase complex. The chain length of the released fatty acid is usually C16. However, in the mammary glands of non-ruminant mammals, and in the uropygial gland of certain waterfowl there exists a second thioesterase which releases medium-chain length fatty acids (C8 to C2) (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The type I fatty acid synthase (FASN) is responsible for the de novo synthesis of palmitate. Chain length selection and release is performed by the C-terminal thioesterase domain (TE1). FASN expression is upregulated in cancer, and its activity levels controlled by gene dosage, transcriptional and post-translational mechanisms. In addition, the chain length of fatty acids produced by FASN is controlled by a type II thioesterase called TE2 (E.C. 3.1.2.14). TE2 has been implicated in breast cancer and generates a broad lipid distribution within milk. The molecular basis for the ability of the TE2 to compete with TE1 for the acyl chain attached to the acyl carrier protein (ACP) domain of FASN is unknown. Herein, we show that human TE1 efficiently hydrolyzes acyl-CoA substrate mimetics. In contrast, TE2 prefers an engineered human acyl-ACP substrate and readily releases short chain fatty acids from full-length FASN during turnover. The 2.8 A crystal structure of TE2 reveals a novel capping domain insert within the alpha/beta hydrolase core. This domain is reminiscent of capping domains of type II thioesterases involved in polyketide synthesis. The structure also reveals that the capping domain had collapsed onto the active site containing the Ser101-His237-Asp212 catalytic triad. This observation suggests that the capping domain opens to enable the ACP domain to dock and to place the acyl chain and 4-phosphopantetheinyl-linker arm correctly for catalysis. Thus, the ability of TE2 to prematurely release fatty acids from FASN parallels the role of editing thioesterases involved in polyketide and non-ribosomal peptide synthase synthases.
		+
		+	Crystal Structure and Substrate Specificity of Human Thioesterase 2: Insights into the Molecular Basis for the Modulation of Fatty Acid Synthase.,Ritchie MK, Johnson LC, Clodfelter JE, Pemble CW 4th, Fulp BE, Furdui CM, Kridel SJ, Lowther WT J Biol Chem. 2015 Dec 9. pii: jbc.M115.702597. PMID:26663084<ref>PMID:26663084</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4xjv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:54, 16 December 2015

OCA — Wed, 16 Dec 2015 13:54:28 GMT

←Older revision		Revision as of 13:54, 16 December 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of human thioesterase 2==
-		+	<StructureSection load='4xjv' size='340' side='right' caption='[[4xjv]], [[Resolution\|resolution]] 2.80Å' scene=''>
-	~~The entry~~ 4xjv is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4xjv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XJV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XJV FirstGlance]. <br>
-	~~Authors~~: ~~Ritchie~~, M.K., ~~Kridel, S~~.J., ~~Lowther~~, W.T.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oleoyl-[acyl-carrier-protein]_hydrolase Oleoyl-[acyl-carrier-protein] hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.14 3.1.2.14] </span></td></tr>
-	~~Description~~: ~~Crystal structure~~ of ~~human~~ thioesterase 2	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xjv OCA], [http://pdbe.org/4xjv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xjv RCSB], [http://www.ebi.ac.uk/pdbsum/4xjv PDBsum]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: Lowther, W.T]]	+	== Function ==
-	[[Category: Ritchie, M.K]]	+	[[http://www.uniprot.org/uniprot/SAST_HUMAN SAST_HUMAN]] In fatty acid biosynthesis chain termination and release of the free fatty acid product is achieved by hydrolysis of the thio ester by a thioesterase I, a component of the fatty acid synthetase complex. The chain length of the released fatty acid is usually C16. However, in the mammary glands of non-ruminant mammals, and in the uropygial gland of certain waterfowl there exists a second thioesterase which releases medium-chain length fatty acids (C8 to C2) (By similarity).
-	[[Category: ~~Kridel, S.J~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Kridel, S J]]
		+	[[Category: Lowther, W T]]
		+	[[Category: Ritchie, M K]]
		+	[[Category: Closed conformation]]
		+	[[Category: Fatty acid synthase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Lipid metabolism]]

OCA at 23:12, 30 November 2015

OCA — Mon, 30 Nov 2015 23:12:32 GMT

←Older revision		Revision as of 23:12, 30 November 2015
Line 8:		Line 8:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Lowther, W.T]]		[[Category: Lowther, W.T]]
-	[[Category: Kridel, S.J]]
	[[Category: Ritchie, M.K]]		[[Category: Ritchie, M.K]]
		+	[[Category: Kridel, S.J]]

OCA at 00:49, 22 October 2015

OCA — Thu, 22 Oct 2015 00:49:15 GMT

←Older revision		Revision as of 00:49, 22 October 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4xjv is ON HOLD	+	The entry 4xjv is ON HOLD until Paper Publication

	Authors: Ritchie, M.K., Kridel, S.J., Lowther, W.T.		Authors: Ritchie, M.K., Kridel, S.J., Lowther, W.T.

OCA at 23:04, 15 October 2015

OCA — Thu, 15 Oct 2015 23:04:41 GMT

←Older revision		Revision as of 23:04, 15 October 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4xjv is ON HOLD ~~until Paper Publication~~	+	The entry 4xjv is ON HOLD

	Authors: Ritchie, M.K., Kridel, S.J., Lowther, W.T.		Authors: Ritchie, M.K., Kridel, S.J., Lowther, W.T.