4xt1 - Revision history

OCA at 07:49, 27 September 2023

OCA — Wed, 27 Sep 2023 07:49:02 GMT

←Older revision		Revision as of 07:49, 27 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4xt1]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cytomegalovirus Cytomegalovirus], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Vicugna_pacos Vicugna pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XT1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XT1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4xt1]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cytomegalovirus Cytomegalovirus], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Vicugna_pacos Vicugna pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XT1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XT1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene>, <scene name='pdbligand=UNL:UNKNOWN+LIGAND'>UNL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.886Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene>, <scene name='pdbligand=UNL:UNKNOWN+LIGAND'>UNL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xt1 OCA], [https://pdbe.org/4xt1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xt1 RCSB], [https://www.ebi.ac.uk/pdbsum/4xt1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xt1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xt1 OCA], [https://pdbe.org/4xt1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xt1 RCSB], [https://www.ebi.ac.uk/pdbsum/4xt1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xt1 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 17:50, 26 April 2023

OCA — Wed, 26 Apr 2023 17:50:54 GMT

←Older revision		Revision as of 17:50, 26 April 2023
Line 3:		Line 3:
	<StructureSection load='4xt1' size='340' side='right'caption='[[4xt1]], [[Resolution\|resolution]] 2.89Å' scene=''>		<StructureSection load='4xt1' size='340' side='right'caption='[[4xt1]], [[Resolution\|resolution]] 2.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4xt1]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Alpaca Alpaca~~], [~~http~~://en.wikipedia.org/wiki/~~Cytomegalovirus Cytomegalovirus~~] and [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XT1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4XT1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4xt1]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cytomegalovirus Cytomegalovirus], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Vicugna_pacos Vicugna pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XT1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XT1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=~~SIN~~:~~SUCCINIC~~+ACID'>~~SIN~~</scene>, <scene name='pdbligand=~~UNL~~:~~UNKNOWN~~+~~LIGAND~~'>~~UNL~~</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene>, <scene name='pdbligand=UNL:UNKNOWN+LIGAND'>UNL</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=~~PCA~~:~~PYROGLUTAMIC~~+~~ACID~~'>~~PCA~~</scene>~~</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xt1 OCA], [https://pdbe.org/4xt1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xt1 RCSB], [https://www.ebi.ac.uk/pdbsum/4xt1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xt1 ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xt3\|4xt3]]</td></tr>~~	+
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CX3CL1, FKN, NTT, SCYD1, A-152E5.2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4xt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xt1 OCA], [~~http~~://pdbe.org/4xt1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4xt1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4xt1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4xt1 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/US28_HCMVA US28_HCMVA]] Receptor for a C-C type chemokine. Binds to a number of different CC-chemokines including CCL5/RANTES, CCL2/MCP-1, CCL3/MIP-1-alpha as well as CX3CL1/Fractalkine. Transduces signals resulting in the activation of MAP kinase signaling pathways and augmentation of intracellular calcium ion levels, leading to alterations in chemotactic behavior of vascular smooth muscle cells and macrophages. The US28 receptor also exhibits high levels of agonist-independent signaling activity and agonist-independent endocytosis. Interacts with endogenous Gaq/11 subunits and thereby constitutively activates phospholipase C.<ref>PMID:11050102</ref> <ref>PMID:7961796</ref> [[http://www.uniprot.org/uniprot/X3CL1_HUMAN X3CL1_HUMAN]] The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.<ref>PMID:21829356</ref>	+	[https://www.uniprot.org/uniprot/US28_HCMVA US28_HCMVA] Receptor for a C-C type chemokine. Binds to a number of different CC-chemokines including CCL5/RANTES, CCL2/MCP-1, CCL3/MIP-1-alpha as well as CX3CL1/Fractalkine. Transduces signals resulting in the activation of MAP kinase signaling pathways and augmentation of intracellular calcium ion levels, leading to alterations in chemotactic behavior of vascular smooth muscle cells and macrophages. The US28 receptor also exhibits high levels of agonist-independent signaling activity and agonist-independent endocytosis. Interacts with endogenous Gaq/11 subunits and thereby constitutively activates phospholipase C.<ref>PMID:11050102</ref> <ref>PMID:7961796</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Alpaca]]
	[[Category: Cytomegalovirus]]		[[Category: Cytomegalovirus]]
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Burg, J S~~]]	+	[[Category: Vicugna pacos]]
-	[[Category: ~~Garcia, K C~~]]	+	[[Category: Burg JS]]
-	[[Category: ~~Jude, K M~~]]	+	[[Category: Garcia KC]]
-	[[Category: ~~Waghray, D~~]]	+	[[Category: Jude KM]]
-	[[Category: ~~Chemokine]]~~	+	[[Category: Waghray D]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Gpcr]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Viral protein-signalling protein complex~~]]	+

OCA at 00:11, 6 June 2019

OCA — Thu, 06 Jun 2019 00:11:52 GMT

←Older revision		Revision as of 00:11, 6 June 2019
Line 1:		Line 1:

	==Structure of a nanobody-bound viral GPCR bound to human chemokine CX3CL1==		==Structure of a nanobody-bound viral GPCR bound to human chemokine CX3CL1==
-	<StructureSection load='4xt1' size='340' side='right' caption='[[4xt1]], [[Resolution\|resolution]] 2.89Å' scene=''>	+	<StructureSection load='4xt1' size='340' side='right'caption='[[4xt1]], [[Resolution\|resolution]] 2.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4xt1]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XT1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XT1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4xt1]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Alpaca Alpaca], [http://en.wikipedia.org/wiki/Cytomegalovirus Cytomegalovirus] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XT1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XT1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene>, <scene name='pdbligand=UNL:UNKNOWN+LIGAND'>UNL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene>, <scene name='pdbligand=UNL:UNKNOWN+LIGAND'>UNL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xt3\|4xt3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xt3\|4xt3]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CX3CL1, FKN, NTT, SCYD1, A-152E5.2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xt1 OCA], [http://pdbe.org/4xt1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xt1 RCSB], [http://www.ebi.ac.uk/pdbsum/4xt1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xt1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xt1 OCA], [http://pdbe.org/4xt1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xt1 RCSB], [http://www.ebi.ac.uk/pdbsum/4xt1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xt1 ProSAT]</span></td></tr>
	</table>		</table>
Line 24:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Alpaca]]
		+	[[Category: Cytomegalovirus]]
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Burg, J S]]		[[Category: Burg, J S]]
	[[Category: Garcia, K C]]		[[Category: Garcia, K C]]

OCA at 18:44, 1 February 2017

OCA — Wed, 01 Feb 2017 18:44:20 GMT

←Older revision		Revision as of 18:44, 1 February 2017
Line 1:		Line 1:
		+
	==Structure of a nanobody-bound viral GPCR bound to human chemokine CX3CL1==		==Structure of a nanobody-bound viral GPCR bound to human chemokine CX3CL1==
	<StructureSection load='4xt1' size='340' side='right' caption='[[4xt1]], [[Resolution\|resolution]] 2.89Å' scene=''>		<StructureSection load='4xt1' size='340' side='right' caption='[[4xt1]], [[Resolution\|resolution]] 2.89Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xt3\|4xt3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xt3\|4xt3]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xt1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xt1 RCSB], [http://www.ebi.ac.uk/pdbsum/4xt1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xt1 OCA], [http://pdbe.org/4xt1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xt1 RCSB], [http://www.ebi.ac.uk/pdbsum/4xt1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xt1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4xt1" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:58, 18 March 2015

OCA — Wed, 18 Mar 2015 11:58:29 GMT

←Older revision		Revision as of 11:58, 18 March 2015
Line 10:		Line 10:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/US28_HCMVA US28_HCMVA]] Receptor for a C-C type chemokine. Binds to a number of different CC-chemokines including CCL5/RANTES, CCL2/MCP-1, CCL3/MIP-1-alpha as well as CX3CL1/Fractalkine. Transduces signals resulting in the activation of MAP kinase signaling pathways and augmentation of intracellular calcium ion levels, leading to alterations in chemotactic behavior of vascular smooth muscle cells and macrophages. The US28 receptor also exhibits high levels of agonist-independent signaling activity and agonist-independent endocytosis. Interacts with endogenous Gaq/11 subunits and thereby constitutively activates phospholipase C.<ref>PMID:11050102</ref> <ref>PMID:7961796</ref> [[http://www.uniprot.org/uniprot/X3CL1_HUMAN X3CL1_HUMAN]] The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.<ref>PMID:21829356</ref>		[[http://www.uniprot.org/uniprot/US28_HCMVA US28_HCMVA]] Receptor for a C-C type chemokine. Binds to a number of different CC-chemokines including CCL5/RANTES, CCL2/MCP-1, CCL3/MIP-1-alpha as well as CX3CL1/Fractalkine. Transduces signals resulting in the activation of MAP kinase signaling pathways and augmentation of intracellular calcium ion levels, leading to alterations in chemotactic behavior of vascular smooth muscle cells and macrophages. The US28 receptor also exhibits high levels of agonist-independent signaling activity and agonist-independent endocytosis. Interacts with endogenous Gaq/11 subunits and thereby constitutively activates phospholipase C.<ref>PMID:11050102</ref> <ref>PMID:7961796</ref> [[http://www.uniprot.org/uniprot/X3CL1_HUMAN X3CL1_HUMAN]] The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.<ref>PMID:21829356</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chemokines are small proteins that function as immune modulators through activation of chemokine G protein-coupled receptors (GPCRs). Several viruses also encode chemokines and chemokine receptors to subvert the host immune response. How protein ligands activate GPCRs remains unknown. We report the crystal structure at 2.9 angstrom resolution of the human cytomegalovirus GPCR US28 in complex with the chemokine domain of human CX3CL1 (fractalkine). The globular body of CX3CL1 is perched on top of the US28 extracellular vestibule, whereas its amino terminus projects into the central core of US28. The transmembrane helices of US28 adopt an active-state-like conformation. Atomic-level simulations suggest that the agonist-independent activity of US28 may be due to an amino acid network evolved in the viral GPCR to destabilize the receptor's inactive state.
		+
		+	Structural biology. Structural basis for chemokine recognition and activation of a viral G protein-coupled receptor.,Burg JS, Ingram JR, Venkatakrishnan AJ, Jude KM, Dukkipati A, Feinberg EN, Angelini A, Waghray D, Dror RO, Ploegh HL, Garcia KC Science. 2015 Mar 6;347(6226):1113-7. doi: 10.1126/science.aaa5026. PMID:25745166<ref>PMID:25745166</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:39, 4 March 2015

OCA — Wed, 04 Mar 2015 11:39:46 GMT

←Older revision		Revision as of 11:39, 4 March 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Structure of a nanobody-bound viral GPCR bound to human chemokine CX3CL1==
-		+	<StructureSection load='4xt1' size='340' side='right' caption='[[4xt1]], [[Resolution\|resolution]] 2.89Å' scene=''>
-	~~The entry~~ 4xt1 is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4xt1]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XT1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XT1 FirstGlance]. <br>
-	~~Authors~~: ~~Burg~~, J.S., ~~Jude~~, K.M., ~~Waghray~~, D., ~~Garcia, K~~.C.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene>, <scene name='pdbligand=UNL:UNKNOWN+LIGAND'>UNL</scene></td></tr>
-		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	~~Description~~:	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xt3\|4xt3]]</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xt1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xt1 RCSB], [http://www.ebi.ac.uk/pdbsum/4xt1 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/US28_HCMVA US28_HCMVA]] Receptor for a C-C type chemokine. Binds to a number of different CC-chemokines including CCL5/RANTES, CCL2/MCP-1, CCL3/MIP-1-alpha as well as CX3CL1/Fractalkine. Transduces signals resulting in the activation of MAP kinase signaling pathways and augmentation of intracellular calcium ion levels, leading to alterations in chemotactic behavior of vascular smooth muscle cells and macrophages. The US28 receptor also exhibits high levels of agonist-independent signaling activity and agonist-independent endocytosis. Interacts with endogenous Gaq/11 subunits and thereby constitutively activates phospholipase C.<ref>PMID:11050102</ref> <ref>PMID:7961796</ref> [[http://www.uniprot.org/uniprot/X3CL1_HUMAN X3CL1_HUMAN]] The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.<ref>PMID:21829356</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Burg, J S]]
		+	[[Category: Garcia, K C]]
		+	[[Category: Jude, K M]]
	[[Category: Waghray, D]]		[[Category: Waghray, D]]
-	[[Category: ~~Jude, K.M~~]]	+	[[Category: Chemokine]]
-	[[Category: ~~Garcia, K.C~~]]	+	[[Category: Complex]]
-	[[Category: ~~Burg, J.S~~]]	+	[[Category: Gpcr]]
		+	[[Category: Membrane protein]]
		+	[[Category: Viral protein-signalling protein complex]]

OCA at 14:47, 7 February 2015

OCA — Sat, 07 Feb 2015 14:47:21 GMT

←Older revision		Revision as of 14:47, 7 February 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4xt1 is ON HOLD	+	The entry 4xt1 is ON HOLD until Paper Publication

-	Authors: Burg, J.S., Jude, K.M., Garcia, K.C.	+	Authors: Burg, J.S., Jude, K.M., Waghray, D., Garcia, K.C.

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Waghray, D]]
	[[Category: Jude, K.M]]		[[Category: Jude, K.M]]
	[[Category: Garcia, K.C]]		[[Category: Garcia, K.C]]
	[[Category: Burg, J.S]]		[[Category: Burg, J.S]]

OCA: Protected "4xt1" [edit=sysop:move=sysop]

OCA — Wed, 28 Jan 2015 15:49:03 GMT

Protected "4xt1" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:49, 28 January 2015

OCA: New page: '''Unreleased structure''' The entry 4xt1 is ON HOLD Authors: Burg, J.S., Jude, K.M., Garcia, K.C. Description: Category: Unreleased Structures Category: Jude, K.M [[Category:...

OCA — Wed, 28 Jan 2015 15:49:02 GMT

New page: '''Unreleased structure''' The entry 4xt1 is ON HOLD Authors: Burg, J.S., Jude, K.M., Garcia, K.C. Description: Category: Unreleased Structures Category: Jude, K.M [[Category:...

New page

'''Unreleased structure'''

The entry 4xt1 is ON HOLD

Authors: Burg, J.S., Jude, K.M., Garcia, K.C.

Description:
[[Category: Unreleased Structures]]
[[Category: Jude, K.M]]
[[Category: Garcia, K.C]]
[[Category: Burg, J.S]]