4xu4 - Revision history

OCA at 09:02, 20 March 2024

OCA — Wed, 20 Mar 2024 09:02:49 GMT

←Older revision		Revision as of 09:02, 20 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4xu4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_vanbaalenii_PYR-1 Mycolicibacterium vanbaalenii PYR-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XU4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XU4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4xu4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_vanbaalenii_PYR-1 Mycolicibacterium vanbaalenii PYR-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XU4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XU4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=DDQ:DECYLAMINE-N,N-DIMETHYL-N-OXIDE'>DDQ</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.901Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=DDQ:DECYLAMINE-N,N-DIMETHYL-N-OXIDE'>DDQ</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xu4 OCA], [https://pdbe.org/4xu4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xu4 RCSB], [https://www.ebi.ac.uk/pdbsum/4xu4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xu4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xu4 OCA], [https://pdbe.org/4xu4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xu4 RCSB], [https://www.ebi.ac.uk/pdbsum/4xu4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xu4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/INSIG_MYCVP INSIG_MYCVP] Diacylglycerol-binding protein.<ref>PMID:26160948</ref>		[https://www.uniprot.org/uniprot/INSIG_MYCVP INSIG_MYCVP] Diacylglycerol-binding protein.<ref>PMID:26160948</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Insulin-induced gene 1 (Insig-1) and Insig-2 are endoplasmic reticulum membrane-embedded sterol sensors that regulate the cellular accumulation of sterols. Despite their physiological importance, the structural information on Insigs remains limited. Here we report the high-resolution structures of MvINS, an Insig homolog from Mycobacterium vanbaalenii. MvINS exists as a homotrimer. Each protomer comprises six transmembrane segments (TMs), with TM3 and TM4 contributing to homotrimerization. The six TMs enclose a V-shaped cavity that can accommodate a diacylglycerol molecule. A homology-based structural model of human Insig-2, together with biochemical characterizations, suggest that the central cavity of Insig-2 accommodates 25-hydroxycholesterol, whereas TM3 and TM4 engage in Scap binding. These analyses provide an important framework for further functional and mechanistic understanding of Insig proteins and the sterol regulatory element-binding protein pathway.
-
-	PROTEIN STRUCTURE. Crystal structure of a mycobacterial Insig homolog provides insight into how these sensors monitor sterol levels.,Ren R, Zhou X, He Y, Ke M, Wu J, Liu X, Yan C, Wu Y, Gong X, Lei X, Yan SF, Radhakrishnan A, Yan N Science. 2015 Jul 10;349(6244):187-91. doi: 10.1126/science.aab1091. PMID:26160948<ref>PMID:26160948</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4xu4" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:52, 26 April 2023

OCA — Wed, 26 Apr 2023 17:52:53 GMT

←Older revision		Revision as of 17:52, 26 April 2023
Line 3:		Line 3:
	<StructureSection load='4xu4' size='340' side='right'caption='[[4xu4]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4xu4' size='340' side='right'caption='[[4xu4]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4xu4]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Mycvp Mycvp~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XU4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4XU4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4xu4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_vanbaalenii_PYR-1 Mycolicibacterium vanbaalenii PYR-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XU4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XU4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=DDQ:DECYLAMINE-N,N-DIMETHYL-N-OXIDE'>DDQ</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=DDQ:DECYLAMINE-N,N-DIMETHYL-N-OXIDE'>DDQ</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xu5\|4xu5]], [[4xu6\|4xu6]], [[4xu7\|4xu7]], [[4xu8\|4xu8]], [[4xu9\|4xu9]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xu4 OCA], [https://pdbe.org/4xu4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xu4 RCSB], [https://www.ebi.ac.uk/pdbsum/4xu4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xu4 ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Mvan_1475 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=350058 MYCVP])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4xu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xu4 OCA], [~~http~~://pdbe.org/4xu4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4xu4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4xu4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4xu4 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/INSIG_MYCVP INSIG_MYCVP] Diacylglycerol-binding protein.<ref>PMID:26160948</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Mycvp~~]]	+	[[Category: Mycolicibacterium vanbaalenii PYR-1]]
-	[[Category: He, Y]]	+	[[Category: He Y]]
-	[[Category: Ren~~, R B~~]]	+	[[Category: Ren RB]]
-	[[Category: Wu~~, J P~~]]	+	[[Category: Wu JP]]
-	[[Category: Yan~~, C Y~~]]	+	[[Category: Yan CY]]
-	[[Category: Yan, N]]	+	[[Category: Yan N]]
-	~~[[Category: Unknown function~~]]	+

OCA at 23:47, 5 June 2019

OCA — Wed, 05 Jun 2019 23:47:20 GMT

←Older revision		Revision as of 23:47, 5 June 2019
Line 1:		Line 1:
		+
	==Crystal structure of a mycobacterial Insig homolog MvINS from Mycobacterium vanbaalenii at 1.9A resolution==		==Crystal structure of a mycobacterial Insig homolog MvINS from Mycobacterium vanbaalenii at 1.9A resolution==
-	<StructureSection load='4xu4' size='340' side='right' caption='[[4xu4]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='4xu4' size='340' side='right'caption='[[4xu4]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4xu4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XU4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XU4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4xu4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycvp Mycvp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XU4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XU4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=DDQ:DECYLAMINE-N,N-DIMETHYL-N-OXIDE'>DDQ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=DDQ:DECYLAMINE-N,N-DIMETHYL-N-OXIDE'>DDQ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xu5\|4xu5]], [[4xu6\|4xu6]], [[4xu7\|4xu7]], [[4xu8\|4xu8]], [[4xu9\|4xu9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xu5\|4xu5]], [[4xu6\|4xu6]], [[4xu7\|4xu7]], [[4xu8\|4xu8]], [[4xu9\|4xu9]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xu4 OCA], [http://pdbe.org/4xu4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xu4 RCSB], [http://www.ebi.ac.uk/pdbsum/4xu4 PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Mvan_1475 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=350058 MYCVP])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xu4 OCA], [http://pdbe.org/4xu4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xu4 RCSB], [http://www.ebi.ac.uk/pdbsum/4xu4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xu4 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Mycvp]]
	[[Category: He, Y]]		[[Category: He, Y]]
	[[Category: Ren, R B]]		[[Category: Ren, R B]]

OCA at 03:54, 16 October 2015

OCA — Fri, 16 Oct 2015 03:54:59 GMT

←Older revision		Revision as of 03:54, 16 October 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of a mycobacterial Insig homolog MvINS from Mycobacterium vanbaalenii at 1.9A resolution==
		+	<StructureSection load='4xu4' size='340' side='right' caption='[[4xu4]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4xu4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XU4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XU4 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=DDQ:DECYLAMINE-N,N-DIMETHYL-N-OXIDE'>DDQ</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xu5\|4xu5]], [[4xu6\|4xu6]], [[4xu7\|4xu7]], [[4xu8\|4xu8]], [[4xu9\|4xu9]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xu4 OCA], [http://pdbe.org/4xu4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xu4 RCSB], [http://www.ebi.ac.uk/pdbsum/4xu4 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Insulin-induced gene 1 (Insig-1) and Insig-2 are endoplasmic reticulum membrane-embedded sterol sensors that regulate the cellular accumulation of sterols. Despite their physiological importance, the structural information on Insigs remains limited. Here we report the high-resolution structures of MvINS, an Insig homolog from Mycobacterium vanbaalenii. MvINS exists as a homotrimer. Each protomer comprises six transmembrane segments (TMs), with TM3 and TM4 contributing to homotrimerization. The six TMs enclose a V-shaped cavity that can accommodate a diacylglycerol molecule. A homology-based structural model of human Insig-2, together with biochemical characterizations, suggest that the central cavity of Insig-2 accommodates 25-hydroxycholesterol, whereas TM3 and TM4 engage in Scap binding. These analyses provide an important framework for further functional and mechanistic understanding of Insig proteins and the sterol regulatory element-binding protein pathway.

-	~~The entry 4xu4 is ON HOLD until Paper Publication~~	+	PROTEIN STRUCTURE. Crystal structure of a mycobacterial Insig homolog provides insight into how these sensors monitor sterol levels.,Ren R, Zhou X, He Y, Ke M, Wu J, Liu X, Yan C, Wu Y, Gong X, Lei X, Yan SF, Radhakrishnan A, Yan N Science. 2015 Jul 10;349(6244):187-91. doi: 10.1126/science.aab1091. PMID:26160948<ref>PMID:26160948</ref>

-	~~Authors: Ren~~, R.B.~~, Wu, J.P., Yan, C.Y., He, Y., Yan, N~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~:	+	<div class="pdbe-citations 4xu4" style="background-color:#fffaf0;"></div>
-	~~[[Category: Unreleased Structures]]~~	+	== References ==
-	~~[[Category: Ren, R.B]]~~	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: He, Y]]		[[Category: He, Y]]
-	[[Category: Wu, J.P]]	+	[[Category: Ren, R B]]
		+	[[Category: Wu, J P]]
		+	[[Category: Yan, C Y]]
	[[Category: Yan, N]]		[[Category: Yan, N]]
-	[[Category: ~~Yan, C.Y~~]]	+	[[Category: Unknown function]]

OCA at 09:41, 30 September 2015

OCA — Wed, 30 Sep 2015 09:41:05 GMT

←Older revision		Revision as of 09:41, 30 September 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4xu4 is ON HOLD	+	The entry 4xu4 is ON HOLD until Paper Publication

	Authors: Ren, R.B., Wu, J.P., Yan, C.Y., He, Y., Yan, N.		Authors: Ren, R.B., Wu, J.P., Yan, C.Y., He, Y., Yan, N.

OCA: Protected "4xu4" [edit=sysop:move=sysop]

OCA — Sat, 07 Feb 2015 14:49:29 GMT

Protected "4xu4" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:49, 7 February 2015

OCA: New page: '''Unreleased structure''' The entry 4xu4 is ON HOLD Authors: Ren, R.B., Wu, J.P., Yan, C.Y., He, Y., Yan, N. Description: Category: Unreleased Structures Category: Ren, R.B [...

OCA — Sat, 07 Feb 2015 14:49:27 GMT

New page: '''Unreleased structure''' The entry 4xu4 is ON HOLD Authors: Ren, R.B., Wu, J.P., Yan, C.Y., He, Y., Yan, N. Description: Category: Unreleased Structures Category: Ren, R.B [...

New page

'''Unreleased structure'''

The entry 4xu4 is ON HOLD

Authors: Ren, R.B., Wu, J.P., Yan, C.Y., He, Y., Yan, N.

Description:
[[Category: Unreleased Structures]]
[[Category: Ren, R.B]]
[[Category: He, Y]]
[[Category: Wu, J.P]]
[[Category: Yan, N]]
[[Category: Yan, C.Y]]