4y0k - Revision history

OCA at 15:33, 8 November 2023

OCA — Wed, 08 Nov 2023 15:33:09 GMT

←Older revision		Revision as of 15:33, 8 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4y0k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._NRRL_2288 Streptomyces sp. NRRL 2288]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y0K FirstGlance]. <br>		<table><tr><td colspan='2'>[[4y0k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._NRRL_2288 Streptomyces sp. NRRL 2288]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y0K FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.203Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y0k OCA], [https://pdbe.org/4y0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y0k RCSB], [https://www.ebi.ac.uk/pdbsum/4y0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y0k ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y0k OCA], [https://pdbe.org/4y0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y0k RCSB], [https://www.ebi.ac.uk/pdbsum/4y0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y0k ProSAT]</span></td></tr>
	</table>		</table>

OCA at 18:01, 26 April 2023

OCA — Wed, 26 Apr 2023 18:01:16 GMT

←Older revision		Revision as of 18:01, 26 April 2023
Line 3:		Line 3:
	<StructureSection load='4y0k' size='340' side='right'caption='[[4y0k]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4y0k' size='340' side='right'caption='[[4y0k]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4y0k]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Streptomyces_sp.~~_nrrl_2288~~ Streptomyces sp. ~~nrrl~~ 2288]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y0K OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4Y0K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4y0k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._NRRL_2288 Streptomyces sp. NRRL 2288]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y0K FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4y1b\|4y1b]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y0k OCA], [https://pdbe.org/4y0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y0k RCSB], [https://www.ebi.ac.uk/pdbsum/4y0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y0k ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">antE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1298930 Streptomyces sp. NRRL 2288])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4y0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y0k OCA], [~~http~~://pdbe.org/4y0k PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4y0k RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4y0k PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4y0k ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/M1SQA1_9ACTN M1SQA1_9ACTN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Streptomyces sp. ~~nrrl~~ 2288]]	+	[[Category: Streptomyces sp. NRRL 2288]]
-	[[Category: Abe, I]]	+	[[Category: Abe I]]
-	[[Category: Mori, T]]	+	[[Category: Mori T]]
-	[[Category: Zhang, L]]	+	[[Category: Zhang L]]
-	~~[[Category: Antimycin biosynthesis]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 08:42, 5 February 2020

OCA — Wed, 05 Feb 2020 08:42:11 GMT

←Older revision		Revision as of 08:42, 5 February 2020
Line 1:		Line 1:

	==Structure of crotonyl-CoA carboxylase/reductase AntE in complex with NADP==		==Structure of crotonyl-CoA carboxylase/reductase AntE in complex with NADP==
-	<StructureSection load='4y0k' size='340' side='right' caption='[[4y0k]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='4y0k' size='340' side='right'caption='[[4y0k]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4y0k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._nrrl_2288 Streptomyces sp. nrrl 2288]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y0K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y0K FirstGlance]. <br>		<table><tr><td colspan='2'>[[4y0k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._nrrl_2288 Streptomyces sp. nrrl 2288]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y0K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y0K FirstGlance]. <br>
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Streptomyces sp. nrrl 2288]]		[[Category: Streptomyces sp. nrrl 2288]]
	[[Category: Abe, I]]		[[Category: Abe, I]]

OCA at 14:29, 16 November 2017

OCA — Thu, 16 Nov 2017 14:29:31 GMT

←Older revision		Revision as of 14:29, 16 November 2017
Line 1:		Line 1:
		+
	==Structure of crotonyl-CoA carboxylase/reductase AntE in complex with NADP==		==Structure of crotonyl-CoA carboxylase/reductase AntE in complex with NADP==
	<StructureSection load='4y0k' size='340' side='right' caption='[[4y0k]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4y0k' size='340' side='right' caption='[[4y0k]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4y0k]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y0K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y0K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4y0k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._nrrl_2288 Streptomyces sp. nrrl 2288]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y0K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y0K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4y1b\|4y1b]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4y1b\|4y1b]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y0k OCA], [http://pdbe.org/4y0k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y0k RCSB], [http://www.ebi.ac.uk/pdbsum/4y0k PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">antE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1298930 Streptomyces sp. NRRL 2288])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y0k OCA], [http://pdbe.org/4y0k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y0k RCSB], [http://www.ebi.ac.uk/pdbsum/4y0k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4y0k ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Streptomyces sp. nrrl 2288]]
	[[Category: Abe, I]]		[[Category: Abe, I]]
	[[Category: Mori, T]]		[[Category: Mori, T]]

OCA at 21:20, 30 November 2015

OCA — Mon, 30 Nov 2015 21:20:06 GMT

←Older revision		Revision as of 21:20, 30 November 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Structure of crotonyl-CoA carboxylase/reductase AntE in complex with NADP==
		+	<StructureSection load='4y0k' size='340' side='right' caption='[[4y0k]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4y0k]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y0K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y0K FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4y1b\|4y1b]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y0k OCA], [http://pdbe.org/4y0k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y0k RCSB], [http://www.ebi.ac.uk/pdbsum/4y0k PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bioengineering of natural product biosynthesis is a powerful approach to expand the structural diversity of bioactive molecules. However, in polyketide biosynthesis, the modification of polyketide extender units, which form the carbon skeletons, has remained challenging. Herein, we report the rational control of polyketide extender units by the structure-based engineering of a crotonyl-CoA carboxylase/reductase (CCR), in the biosynthesis of antimycin. Site-directed mutagenesis of the CCR enzyme AntE, guided by the crystal structure solved at 1.5 A resolution, expanded its substrate scope to afford indolylmethylmalonyl-CoA by the V350G mutation. The mutant A182L selectively catalyzed carboxylation over the regular reduction. Furthermore, the combinatorial biosynthesis of heterocycle- and substituted arene-bearing antimycins was achieved by an engineered Streptomyces strain bearing AntE(V350G) . These findings deepen our understanding of the molecular mechanisms of the CCRs, which will serve as versatile biocatalysts for the manipulation of building blocks, and set the stage for the rational design of polyketide biosynthesis.

-	~~The entry 4y0k is ON HOLD until Paper Publication~~	+	Rational Control of Polyketide Extender Units by Structure-Based Engineering of a Crotonyl-CoA Carboxylase/Reductase in Antimycin Biosynthesis.,Zhang L, Mori T, Zheng Q, Awakawa T, Yan Y, Liu W, Abe I Angew Chem Int Ed Engl. 2015 Nov 2;54(45):13462-5. doi: 10.1002/anie.201506899., Epub 2015 Sep 10. PMID:26356484<ref>PMID:26356484</ref>

-	~~Authors: Abe~~, I.~~, Zhang, L~~.~~, Mori, T~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Structure of crotonyl~~-~~CoA carboxylase~~/~~reductase AntE in complex with NADP~~	+	<div class="pdbe-citations 4y0k" style="background-color:#fffaf0;"></div>
-	~~[[Category: Unreleased Structures]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Abe, I]]		[[Category: Abe, I]]
	[[Category: Mori, T]]		[[Category: Mori, T]]
	[[Category: Zhang, L]]		[[Category: Zhang, L]]
		+	[[Category: Antimycin biosynthesis]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Transferase]]

OCA at 12:33, 25 February 2015

OCA — Wed, 25 Feb 2015 12:33:34 GMT

←Older revision		Revision as of 12:33, 25 February 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4y0k is ON HOLD	+	The entry 4y0k is ON HOLD until Paper Publication

	Authors: Abe, I., Zhang, L., Mori, T.		Authors: Abe, I., Zhang, L., Mori, T.

OCA: Protected "4y0k" [edit=sysop:move=sysop]

OCA — Thu, 12 Feb 2015 10:58:02 GMT

Protected "4y0k" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:58, 12 February 2015

OCA: New page: '''Unreleased structure''' The entry 4y0k is ON HOLD Authors: Abe, I., Zhang, L., Mori, T. Description: Structure of crotonyl-CoA carboxylase/reductase AntE in complex with NADP [[Cate...

OCA — Thu, 12 Feb 2015 10:58:01 GMT

New page: '''Unreleased structure''' The entry 4y0k is ON HOLD Authors: Abe, I., Zhang, L., Mori, T. Description: Structure of crotonyl-CoA carboxylase/reductase AntE in complex with NADP [[Cate...

New page

'''Unreleased structure'''

The entry 4y0k is ON HOLD

Authors: Abe, I., Zhang, L., Mori, T.

Description: Structure of crotonyl-CoA carboxylase/reductase AntE in complex with NADP
[[Category: Unreleased Structures]]
[[Category: Abe, I]]
[[Category: Mori, T]]
[[Category: Zhang, L]]