4y1l - Revision history

OCA at 13:02, 1 March 2024

OCA — Fri, 01 Mar 2024 13:02:26 GMT

←Older revision		Revision as of 13:02, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4y1l]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y1L FirstGlance]. <br>		<table><tr><td colspan='2'>[[4y1l]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y1L FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y1l OCA], [https://pdbe.org/4y1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y1l RCSB], [https://www.ebi.ac.uk/pdbsum/4y1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y1l ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y1l OCA], [https://pdbe.org/4y1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y1l RCSB], [https://www.ebi.ac.uk/pdbsum/4y1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y1l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/UBC9_HUMAN UBC9_HUMAN] Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.<ref>PMID:8668529</ref> <ref>PMID:11451954</ref> <ref>PMID:15809060</ref> <ref>PMID:19744555</ref> <ref>PMID:19638400</ref> <ref>PMID:17466333</ref> <ref>PMID:20077568</ref>		[https://www.uniprot.org/uniprot/UBC9_HUMAN UBC9_HUMAN] Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.<ref>PMID:8668529</ref> <ref>PMID:11451954</ref> <ref>PMID:15809060</ref> <ref>PMID:19744555</ref> <ref>PMID:19638400</ref> <ref>PMID:17466333</ref> <ref>PMID:20077568</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	An RWD domain is a well-conserved domain found through bioinformatic analysis of the human proteome sequence; however its function has been unknown. Ubiquitin-like modifications require the catalysis of three enzymes generally known as E1, E2 and E3. We solved the crystal structure of the E2 for the small ubiquitin-like modifiers (SUMO) in complex with an RWD domain, and confirmed the structure using solution NMR analysis. RWD's binding surface on Ubc9 is located near the N-terminus of Ubc9 that is known to be involved in non-covalent binding of the proteins in the conjugation machinery, including a domain of E1, SUMO, and an E3 ligase. NMR data indicates that the RWD domain does not bind to SUMO and E1. The interaction between RWD and Ubc9 has a Kd of 32.30+/-3.86 microM. Consistent with the structure and binding affinity and in contrast to a previous report, the RWD domain and RWDD3 have minimal effects on global SUMOylation. The structural and biochemical information presented here forms the basis for further investigation of the functions of RWD containing proteins.
-
-	RWD Domain as an E2 (Ubc9)-Interaction Module.,Alontaga AY, Ambaye ND, Li YJ, Vega R, Chen CH, Bzymek KP, Williams JC, Hu W, Chen Y J Biol Chem. 2015 Apr 27. pii: jbc.M115.644047. PMID:25918163<ref>PMID:25918163</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4y1l" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 18:03, 26 April 2023

OCA — Wed, 26 Apr 2023 18:03:08 GMT

←Older revision		Revision as of 18:03, 26 April 2023
Line 3:		Line 3:
	<StructureSection load='4y1l' size='340' side='right'caption='[[4y1l]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4y1l' size='340' side='right'caption='[[4y1l]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4y1l]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y1L OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4Y1L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4y1l]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y1L FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UBE2I, UBC9, UBCE9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), RWDD3, RSUME ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y1l OCA], [https://pdbe.org/4y1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y1l RCSB], [https://www.ebi.ac.uk/pdbsum/4y1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y1l ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4y1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y1l OCA], [~~http~~://pdbe.org/4y1l PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4y1l RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4y1l PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4y1l ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/UBC9_HUMAN UBC9_HUMAN]] Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.<ref>PMID:8668529</ref> <ref>PMID:11451954</ref> <ref>PMID:15809060</ref> <ref>PMID:19744555</ref> <ref>PMID:19638400</ref> <ref>PMID:17466333</ref> <ref>PMID:20077568</ref> [[http://www.uniprot.org/uniprot/RWDD3_HUMAN RWDD3_HUMAN]] Enhances the sumoylation of a number of proteins including HIF1A, PIAS and I-kappa-B, through direct interaction with UBC9. Has no effect on ubiquitination.<ref>PMID:17956732</ref>	+	[https://www.uniprot.org/uniprot/UBC9_HUMAN UBC9_HUMAN] Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.<ref>PMID:8668529</ref> <ref>PMID:11451954</ref> <ref>PMID:15809060</ref> <ref>PMID:19744555</ref> <ref>PMID:19638400</ref> <ref>PMID:17466333</ref> <ref>PMID:20077568</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Aileen~~, Y A~~]]	+	[[Category: Aileen YA]]
-	[[Category: Ambaye~~, N D~~]]	+	[[Category: Ambaye ND]]
-	[[Category: Bzymek, K]]	+	[[Category: Bzymek K]]
-	[[Category: Chen, Y]]	+	[[Category: Chen Y]]
-	[[Category: Hu, W]]	+	[[Category: Hu W]]
-	[[Category: Li~~, Y J~~]]	+	[[Category: Li YJ]]
-	[[Category: Vega, R]]	+	[[Category: Vega R]]
-	[[Category: Williams~~, J C]]~~	+	[[Category: Williams JC]]
-	~~[[Category: Homodimer]]~~	+
-	~~[[Category: Ligase-nuclear protein complex]]~~	+
-	~~[[Category: Rwd]]~~	+
-	~~[[Category: Sumo]]~~	+
-	~~[[Category: Sumoylation]]~~	+
-	~~[[Category: Ubc9~~]]	+

OCA at 10:42, 25 December 2019

OCA — Wed, 25 Dec 2019 10:42:11 GMT

←Older revision		Revision as of 10:42, 25 December 2019
Line 1:		Line 1:

	==Ubc9 Homodimer The Missing Link in Poly-SUMO Chain Formation==		==Ubc9 Homodimer The Missing Link in Poly-SUMO Chain Formation==
-	<StructureSection load='4y1l' size='340' side='right' caption='[[4y1l]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='4y1l' size='340' side='right'caption='[[4y1l]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4y1l]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y1L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y1L FirstGlance]. <br>		<table><tr><td colspan='2'>[[4y1l]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y1L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y1L FirstGlance]. <br>
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4y1l" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4y1l" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[SUMO conjugating enzyme Ubc9\|SUMO conjugating enzyme Ubc9]]
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Aileen, Y A]]		[[Category: Aileen, Y A]]
	[[Category: Ambaye, N D]]		[[Category: Ambaye, N D]]

OCA at 15:38, 15 November 2017

OCA — Wed, 15 Nov 2017 15:38:03 GMT

←Older revision		Revision as of 15:38, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='4y1l' size='340' side='right' caption='[[4y1l]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4y1l' size='340' side='right' caption='[[4y1l]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4y1l]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y1L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y1L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4y1l]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y1L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y1L FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y1l OCA], [http://pdbe.org/4y1l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y1l RCSB], [http://www.ebi.ac.uk/pdbsum/4y1l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4y1l ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UBE2I, UBC9, UBCE9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), RWDD3, RSUME ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y1l OCA], [http://pdbe.org/4y1l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y1l RCSB], [http://www.ebi.ac.uk/pdbsum/4y1l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4y1l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4y1l" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4y1l" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[SUMO conjugating enzyme Ubc9\|SUMO conjugating enzyme Ubc9]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Aileen, Y A]]		[[Category: Aileen, Y A]]
	[[Category: Ambaye, N D]]		[[Category: Ambaye, N D]]

OCA at 13:29, 19 April 2017

OCA — Wed, 19 Apr 2017 13:29:19 GMT

←Older revision		Revision as of 13:29, 19 April 2017
Line 1:		Line 1:
		+
	==Ubc9 Homodimer The Missing Link in Poly-SUMO Chain Formation==		==Ubc9 Homodimer The Missing Link in Poly-SUMO Chain Formation==
	<StructureSection load='4y1l' size='340' side='right' caption='[[4y1l]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4y1l' size='340' side='right' caption='[[4y1l]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4y1l]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y1L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y1L FirstGlance]. <br>		<table><tr><td colspan='2'>[[4y1l]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y1L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y1L FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y1l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4y1l RCSB], [http://www.ebi.ac.uk/pdbsum/4y1l PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y1l OCA], [http://pdbe.org/4y1l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y1l RCSB], [http://www.ebi.ac.uk/pdbsum/4y1l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4y1l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4y1l" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 07:45, 15 July 2015

OCA — Wed, 15 Jul 2015 07:45:28 GMT

←Older revision		Revision as of 07:45, 15 July 2015
Line 15:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[SUMO conjugating enzyme Ubc9\|SUMO conjugating enzyme Ubc9]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:26, 13 May 2015

OCA — Wed, 13 May 2015 06:26:50 GMT

←Older revision		Revision as of 06:26, 13 May 2015
Line 7:		Line 7:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/UBC9_HUMAN UBC9_HUMAN]] Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.<ref>PMID:8668529</ref> <ref>PMID:11451954</ref> <ref>PMID:15809060</ref> <ref>PMID:19744555</ref> <ref>PMID:19638400</ref> <ref>PMID:17466333</ref> <ref>PMID:20077568</ref> [[http://www.uniprot.org/uniprot/RWDD3_HUMAN RWDD3_HUMAN]] Enhances the sumoylation of a number of proteins including HIF1A, PIAS and I-kappa-B, through direct interaction with UBC9. Has no effect on ubiquitination.<ref>PMID:17956732</ref>		[[http://www.uniprot.org/uniprot/UBC9_HUMAN UBC9_HUMAN]] Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.<ref>PMID:8668529</ref> <ref>PMID:11451954</ref> <ref>PMID:15809060</ref> <ref>PMID:19744555</ref> <ref>PMID:19638400</ref> <ref>PMID:17466333</ref> <ref>PMID:20077568</ref> [[http://www.uniprot.org/uniprot/RWDD3_HUMAN RWDD3_HUMAN]] Enhances the sumoylation of a number of proteins including HIF1A, PIAS and I-kappa-B, through direct interaction with UBC9. Has no effect on ubiquitination.<ref>PMID:17956732</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	An RWD domain is a well-conserved domain found through bioinformatic analysis of the human proteome sequence; however its function has been unknown. Ubiquitin-like modifications require the catalysis of three enzymes generally known as E1, E2 and E3. We solved the crystal structure of the E2 for the small ubiquitin-like modifiers (SUMO) in complex with an RWD domain, and confirmed the structure using solution NMR analysis. RWD's binding surface on Ubc9 is located near the N-terminus of Ubc9 that is known to be involved in non-covalent binding of the proteins in the conjugation machinery, including a domain of E1, SUMO, and an E3 ligase. NMR data indicates that the RWD domain does not bind to SUMO and E1. The interaction between RWD and Ubc9 has a Kd of 32.30+/-3.86 microM. Consistent with the structure and binding affinity and in contrast to a previous report, the RWD domain and RWDD3 have minimal effects on global SUMOylation. The structural and biochemical information presented here forms the basis for further investigation of the functions of RWD containing proteins.
		+
		+	RWD Domain as an E2 (Ubc9)-Interaction Module.,Alontaga AY, Ambaye ND, Li YJ, Vega R, Chen CH, Bzymek KP, Williams JC, Hu W, Chen Y J Biol Chem. 2015 Apr 27. pii: jbc.M115.644047. PMID:25918163<ref>PMID:25918163</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:03, 6 May 2015

OCA — Wed, 06 May 2015 13:03:21 GMT

←Older revision		Revision as of 13:03, 6 May 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Ubc9 Homodimer The Missing Link in Poly-SUMO Chain Formation==
-		+	<StructureSection load='4y1l' size='340' side='right' caption='[[4y1l]], [[Resolution\|resolution]] 2.70Å' scene=''>
-	~~The entry~~ 4y1l is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4y1l]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y1L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y1L FirstGlance]. <br>
-	~~Authors~~: ~~Aileen, Y~~.A.~~, Ambaye, N~~.D., ~~Li, Y~~.J.~~, Vega, R~~., ~~Bzymek, K~~., ~~Williams, J~~.C., Hu, W., ~~Chen~~, Y.	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y1l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4y1l RCSB], [http://www.ebi.ac.uk/pdbsum/4y1l PDBsum]</span></td></tr>
-		+	</table>
-	~~Description~~: ~~Ubc9 Homodimer The Missing Link in Poly-SUMO Chain Formation~~	+	== Function ==
-	[[Category: ~~Unreleased Structures~~]]	+	[[http://www.uniprot.org/uniprot/UBC9_HUMAN UBC9_HUMAN]] Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.<ref>PMID:8668529</ref> <ref>PMID:11451954</ref> <ref>PMID:15809060</ref> <ref>PMID:19744555</ref> <ref>PMID:19638400</ref> <ref>PMID:17466333</ref> <ref>PMID:20077568</ref> [[http://www.uniprot.org/uniprot/RWDD3_HUMAN RWDD3_HUMAN]] Enhances the sumoylation of a number of proteins including HIF1A, PIAS and I-kappa-B, through direct interaction with UBC9. Has no effect on ubiquitination.<ref>PMID:17956732</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Aileen, Y A]]
		+	[[Category: Ambaye, N D]]
	[[Category: Bzymek, K]]		[[Category: Bzymek, K]]
	[[Category: Chen, Y]]		[[Category: Chen, Y]]
	[[Category: Hu, W]]		[[Category: Hu, W]]
-	[[Category: Li, Y.J]]	+	[[Category: Li, Y J]]
-	~~[[Category: Ambaye, N.D]]~~	+
-	~~[[Category: Aileen, Y.A]]~~	+
-	~~[[Category: Williams, J.C~~]]	+
	[[Category: Vega, R]]		[[Category: Vega, R]]
		+	[[Category: Williams, J C]]
		+	[[Category: Homodimer]]
		+	[[Category: Ligase-nuclear protein complex]]
		+	[[Category: Rwd]]
		+	[[Category: Sumo]]
		+	[[Category: Sumoylation]]
		+	[[Category: Ubc9]]

OCA at 10:00, 4 March 2015

OCA — Wed, 04 Mar 2015 10:00:24 GMT

←Older revision		Revision as of 10:00, 4 March 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4y1l is ON HOLD	+	The entry 4y1l is ON HOLD until Paper Publication

	Authors: Aileen, Y.A., Ambaye, N.D., Li, Y.J., Vega, R., Bzymek, K., Williams, J.C., Hu, W., Chen, Y.		Authors: Aileen, Y.A., Ambaye, N.D., Li, Y.J., Vega, R., Bzymek, K., Williams, J.C., Hu, W., Chen, Y.

OCA at 12:35, 25 February 2015

OCA — Wed, 25 Feb 2015 12:35:22 GMT

←Older revision		Revision as of 12:35, 25 February 2015
Line 3:		Line 3:
	The entry 4y1l is ON HOLD		The entry 4y1l is ON HOLD

-	Authors: Aileen, Y.A, Ambaye, N.D., Li, Y.J., Vega, R., Bzymek, K., Williams, J.C., Hu, W., Chen, Y.	+	Authors: Aileen, Y.A., Ambaye, N.D., Li, Y.J., Vega, R., Bzymek, K., Williams, J.C., Hu, W., Chen, Y.

	Description: Ubc9 Homodimer The Missing Link in Poly-SUMO Chain Formation		Description: Ubc9 Homodimer The Missing Link in Poly-SUMO Chain Formation
Line 11:		Line 11:
	[[Category: Hu, W]]		[[Category: Hu, W]]
	[[Category: Li, Y.J]]		[[Category: Li, Y.J]]
		+	[[Category: Ambaye, N.D]]
		+	[[Category: Aileen, Y.A]]
	[[Category: Williams, J.C]]		[[Category: Williams, J.C]]
	[[Category: Vega, R]]		[[Category: Vega, R]]
-	[[Category: Aileen, Y.A, Ambaye, N.D]]