4y97 - Revision history

OCA at 07:54, 27 September 2023

OCA — Wed, 27 Sep 2023 07:54:57 GMT

←Older revision		Revision as of 07:54, 27 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4y97]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y97 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y97 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4y97]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y97 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y97 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.51Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y97 OCA], [https://pdbe.org/4y97 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y97 RCSB], [https://www.ebi.ac.uk/pdbsum/4y97 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y97 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y97 OCA], [https://pdbe.org/4y97 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y97 RCSB], [https://www.ebi.ac.uk/pdbsum/4y97 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y97 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:03, 3 May 2023

OCA — Wed, 03 May 2023 08:03:58 GMT

←Older revision		Revision as of 08:03, 3 May 2023
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	<StructureSection load='4y97' size='340' side='right'caption='[[4y97]], [[Resolution\|resolution]] 2.51Å' scene=''>		<StructureSection load='4y97' size='340' side='right'caption='[[4y97]], [[Resolution\|resolution]] 2.51Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4y97]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y97 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4Y97 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4y97]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y97 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y97 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POLA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), POLA1, POLA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y97 OCA], [https://pdbe.org/4y97 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y97 RCSB], [https://www.ebi.ac.uk/pdbsum/4y97 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y97 ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4y97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y97 OCA], [~~http~~://pdbe.org/4y97 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4y97 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4y97 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4y97 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/DPOA2_HUMAN DPOA2_HUMAN]] May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity). [[http://www.uniprot.org/uniprot/DPOLA_HUMAN DPOLA_HUMAN]] Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.<ref>PMID:9518481</ref>	+	[https://www.uniprot.org/uniprot/DPOA2_HUMAN DPOA2_HUMAN] May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~DNA-directed DNA polymerase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Babayeva~~, N D~~]]	+	[[Category: Babayeva ND]]
-	[[Category: Baranovskiy~~, A G~~]]	+	[[Category: Baranovskiy AG]]
-	[[Category: Gu, J]]	+	[[Category: Gu J]]
-	[[Category: Suwa, Y]]	+	[[Category: Suwa Y]]
-	[[Category: Tahirov~~, T H]]~~	+	[[Category: Tahirov TH]]
-	~~[[Category: Human dna polymerase alpha]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:45, 25 December 2019

OCA — Wed, 25 Dec 2019 10:45:25 GMT

←Older revision		Revision as of 10:45, 25 December 2019
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	<StructureSection load='4y97' size='340' side='right'caption='[[4y97]], [[Resolution\|resolution]] 2.51Å' scene=''>		<StructureSection load='4y97' size='340' side='right'caption='[[4y97]], [[Resolution\|resolution]] 2.51Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4y97]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y97 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y97 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4y97]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y97 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y97 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POLA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), POLA1, POLA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y97 OCA], [http://pdbe.org/4y97 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y97 RCSB], [http://www.ebi.ac.uk/pdbsum/4y97 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4y97 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y97 OCA], [http://pdbe.org/4y97 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y97 RCSB], [http://www.ebi.ac.uk/pdbsum/4y97 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4y97 ProSAT]</span></td></tr>
Line 27:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Babayeva, N D]]		[[Category: Babayeva, N D]]

OCA at 07:18, 10 July 2019

OCA — Wed, 10 Jul 2019 07:18:18 GMT

←Older revision		Revision as of 07:18, 10 July 2019
Line 1:		Line 1:

	==Crystal Structure of human Pol alpha B-subunit in complex with C-terminal domain of catalytic subunit==		==Crystal Structure of human Pol alpha B-subunit in complex with C-terminal domain of catalytic subunit==
-	<StructureSection load='4y97' size='340' side='right' caption='[[4y97]], [[Resolution\|resolution]] 2.51Å' scene=''>	+	<StructureSection load='4y97' size='340' side='right'caption='[[4y97]], [[Resolution\|resolution]] 2.51Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4y97]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y97 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y97 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4y97]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y97 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y97 FirstGlance]. <br>
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 4y97" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4y97" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DNA polymerase 3D structures\|DNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
		+	[[Category: Large Structures]]
	[[Category: Babayeva, N D]]		[[Category: Babayeva, N D]]
	[[Category: Baranovskiy, A G]]		[[Category: Baranovskiy, A G]]

OCA at 17:08, 22 March 2017

OCA — Wed, 22 Mar 2017 17:08:38 GMT

←Older revision		Revision as of 17:08, 22 March 2017
Line 1:		Line 1:
		+
	==Crystal Structure of human Pol alpha B-subunit in complex with C-terminal domain of catalytic subunit==		==Crystal Structure of human Pol alpha B-subunit in complex with C-terminal domain of catalytic subunit==
	<StructureSection load='4y97' size='340' side='right' caption='[[4y97]], [[Resolution\|resolution]] 2.51Å' scene=''>		<StructureSection load='4y97' size='340' side='right' caption='[[4y97]], [[Resolution\|resolution]] 2.51Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y97 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4y97 RCSB], [http://www.ebi.ac.uk/pdbsum/4y97 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y97 OCA], [http://pdbe.org/4y97 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y97 RCSB], [http://www.ebi.ac.uk/pdbsum/4y97 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4y97 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4y97" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:52, 22 April 2015

OCA — Wed, 22 Apr 2015 06:52:35 GMT

←Older revision		Revision as of 06:52, 22 April 2015
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/DPOA2_HUMAN DPOA2_HUMAN]] May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity). [[http://www.uniprot.org/uniprot/DPOLA_HUMAN DPOLA_HUMAN]] Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.<ref>PMID:9518481</ref>		[[http://www.uniprot.org/uniprot/DPOA2_HUMAN DPOA2_HUMAN]] May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity). [[http://www.uniprot.org/uniprot/DPOLA_HUMAN DPOLA_HUMAN]] Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.<ref>PMID:9518481</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In eukaryotic DNA replication, short RNA-DNA hybrid primers synthesized by primase-DNA polymerase alpha (Prim-Pol alpha) are needed to start DNA replication by the replicative DNA polymerases, Pol delta and Pol . The C-terminus of the Pol alpha catalytic subunit (p180C) in complex with the B subunit (p70) regulates the RNA-priming and DNA-polymerizing activities of Prim-Pol alpha. It tethers Pol alpha and primase, facilitating RNA primer handover from primase to Pol alpha. In order to understand these regulatory mechanisms and to reveal the details of human Pol alpha organization, we determined the crystal structure of p70 in complex with p180C. The structured portion of p70 includes a phosphodiesterase (PDE) domain and an oligonucleotide/oligosaccharide-binding (OB) domain. The N-terminal domain (NTD) and the linker connecting it to the PDE domain are disordered in the reported crystal structure. The p180C adopts an elongated asymmetric saddle shape, with a three-helix bundle in the middle and zinc-binding modules (Zn1 and Zn2) on each side. The extensive p180C-p70 interactions involve 20 hydrogen bonds and a number of hydrophobic interactions resulting in an extended buried surface of 4080 A2. Importantly, in the structure of the p180C-p70 complex with full-length p70, the residues from the N-terminal to the OB-fold domain contribute to interactions with p180C. The comparative structural analysis revealed both the conserved features and the differences between the human and yeast Pol alpha complexes.
		+
		+	Crystal Structure of the Human Pol alpha B Subunit in Complex with the C-terminal Domain of the Catalytic Subunit.,Suwa Y, Gu J, Baranovskiy AG, Babayeva ND, Pavlov YI, Tahirov TH J Biol Chem. 2015 Apr 6. pii: jbc.M115.649954. PMID:25847248<ref>PMID:25847248</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:03, 15 April 2015

OCA — Wed, 15 Apr 2015 13:03:50 GMT

←Older revision		Revision as of 13:03, 15 April 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal Structure of human Pol alpha B-subunit in complex with C-terminal domain of catalytic subunit==
-		+	<StructureSection load='4y97' size='340' side='right' caption='[[4y97]], [[Resolution\|resolution]] 2.51Å' scene=''>
-	~~The entry~~ 4y97 is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4y97]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y97 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y97 FirstGlance]. <br>
-	~~Authors~~: ~~Suwa~~, Y., Gu, J., ~~Baranovskiy, A~~.G., ~~Babayeva~~, N.D., ~~Tahirov~~, ~~T.H~~.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
-	~~Description:~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y97 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4y97 RCSB], [http://www.ebi.ac.uk/pdbsum/4y97 PDBsum]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: Babayeva, N.D]]	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DPOA2_HUMAN DPOA2_HUMAN]] May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity). [[http://www.uniprot.org/uniprot/DPOLA_HUMAN DPOLA_HUMAN]] Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.<ref>PMID:9518481</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: DNA-directed DNA polymerase]]
		+	[[Category: Babayeva, N D]]
		+	[[Category: Baranovskiy, A G]]
	[[Category: Gu, J]]		[[Category: Gu, J]]
-	[[Category: Baranovskiy, A.G]]
-	[[Category: Tahirov, T.H]]
	[[Category: Suwa, Y]]		[[Category: Suwa, Y]]
		+	[[Category: Tahirov, T H]]
		+	[[Category: Human dna polymerase alpha]]
		+	[[Category: Transferase]]

OCA at 11:19, 1 April 2015

OCA — Wed, 01 Apr 2015 11:19:38 GMT

←Older revision		Revision as of 11:19, 1 April 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4y97 is ON HOLD	+	The entry 4y97 is ON HOLD until Paper Publication

	Authors: Suwa, Y., Gu, J., Baranovskiy, A.G., Babayeva, N.D., Tahirov, T.H.		Authors: Suwa, Y., Gu, J., Baranovskiy, A.G., Babayeva, N.D., Tahirov, T.H.

OCA: Protected "4y97" [edit=sysop:move=sysop]

OCA — Wed, 25 Feb 2015 12:50:17 GMT

Protected "4y97" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:50, 25 February 2015

OCA: New page: '''Unreleased structure''' The entry 4y97 is ON HOLD Authors: Suwa, Y., Gu, J., Baranovskiy, A.G., Babayeva, N.D., Tahirov, T.H. Description: Category: Unreleased Structures [[Cat...

OCA — Wed, 25 Feb 2015 12:50:16 GMT

New page: '''Unreleased structure''' The entry 4y97 is ON HOLD Authors: Suwa, Y., Gu, J., Baranovskiy, A.G., Babayeva, N.D., Tahirov, T.H. Description: Category: Unreleased Structures [[Cat...

New page

'''Unreleased structure'''

The entry 4y97 is ON HOLD

Authors: Suwa, Y., Gu, J., Baranovskiy, A.G., Babayeva, N.D., Tahirov, T.H.

Description:
[[Category: Unreleased Structures]]
[[Category: Babayeva, N.D]]
[[Category: Gu, J]]
[[Category: Baranovskiy, A.G]]
[[Category: Tahirov, T.H]]
[[Category: Suwa, Y]]