4ynb - Revision history

OCA at 08:02, 27 September 2023

OCA — Wed, 27 Sep 2023 08:02:22 GMT

←Older revision		Revision as of 08:02, 27 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ynb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_J99 Helicobacter pylori J99]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YNB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YNB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ynb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_J99 Helicobacter pylori J99]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YNB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YNB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4EH:(3R,4S)-1-[(4-AMINO-5H-PYRROLO[3,2-D]PYRIMIDIN-7-YL)METHYL]-4-[(PYRAZIN-2-YLSULFANYL)METHYL]PYRROLIDIN-3-OL'>4EH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4EH:(3R,4S)-1-[(4-AMINO-5H-PYRROLO[3,2-D]PYRIMIDIN-7-YL)METHYL]-4-[(PYRAZIN-2-YLSULFANYL)METHYL]PYRROLIDIN-3-OL'>4EH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ynb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ynb OCA], [https://pdbe.org/4ynb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ynb RCSB], [https://www.ebi.ac.uk/pdbsum/4ynb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ynb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ynb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ynb OCA], [https://pdbe.org/4ynb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ynb RCSB], [https://www.ebi.ac.uk/pdbsum/4ynb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ynb ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:21, 3 May 2023

OCA — Wed, 03 May 2023 08:21:13 GMT

←Older revision		Revision as of 08:21, 3 May 2023
Line 3:		Line 3:
	<StructureSection load='4ynb' size='340' side='right'caption='[[4ynb]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4ynb' size='340' side='right'caption='[[4ynb]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ynb]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Campylobacter_pylori_j99 Campylobacter~~ pylori ~~j99~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YNB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4YNB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ynb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_J99 Helicobacter pylori J99]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YNB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YNB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4EH:(3R,4S)-1-[(4-AMINO-5H-PYRROLO[3,2-D]PYRIMIDIN-7-YL)METHYL]-4-[(PYRAZIN-2-YLSULFANYL)METHYL]PYRROLIDIN-3-OL'>4EH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4EH:(3R,4S)-1-[(4-AMINO-5H-PYRROLO[3,2-D]PYRIMIDIN-7-YL)METHYL]-4-[(PYRAZIN-2-YLSULFANYL)METHYL]PYRROLIDIN-3-OL'>4EH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wkn\|4wkn]], [[4wko\|4wko]], [[4wkp\|4wkp]], [[4ffs\|4ffs]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ynb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ynb OCA], [https://pdbe.org/4ynb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ynb RCSB], [https://www.ebi.ac.uk/pdbsum/4ynb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ynb ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mtnN, mtn, jhp_0082 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85963 Campylobacter pylori J99])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ynb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ynb OCA], [~~http~~://pdbe.org/4ynb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ynb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ynb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ynb ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MQMTN_HELPJ MQMTN_HELPJ]] Catalyzes the direct conversion of aminodeoxyfutalosine (AFL) into dehypoxanthine futalosine (DHFL) and adenine via the hydrolysis of the N-glycosidic bond; this reaction seems to represent an essential step in the menaquinone biosynthesis pathway in Helicobacter species. Also catalyzes the hydrolysis of 5'-methylthioadenosine (MTA) to adenine and 5'-methylthioribose. Can also probably use S-adenosylhomocysteine (SAH) as substrate, leading to adenine and S-ribosylhomocysteine. These other activities highlight the tremendous versatility of the enzyme, which also plays key roles in S-adenosylmethionine recycling and in the biosynthesis of the quorum-sensing molecule autoinducer-2.<ref>PMID:20954236</ref> <ref>PMID:22891633</ref>	+	[https://www.uniprot.org/uniprot/MQMTN_HELPJ MQMTN_HELPJ] Catalyzes the direct conversion of aminodeoxyfutalosine (AFL) into dehypoxanthine futalosine (DHFL) and adenine via the hydrolysis of the N-glycosidic bond; this reaction seems to represent an essential step in the menaquinone biosynthesis pathway in Helicobacter species. Also catalyzes the hydrolysis of 5'-methylthioadenosine (MTA) to adenine and 5'-methylthioribose. Can also probably use S-adenosylhomocysteine (SAH) as substrate, leading to adenine and S-ribosylhomocysteine. These other activities highlight the tremendous versatility of the enzyme, which also plays key roles in S-adenosylmethionine recycling and in the biosynthesis of the quorum-sensing molecule autoinducer-2.<ref>PMID:20954236</ref> <ref>PMID:22891633</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Campylobacter~~ pylori ~~j99~~]]	+	[[Category: Helicobacter pylori J99]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Almo~~, S C~~]]	+	[[Category: Almo SC]]
-	[[Category: Cameron~~, S A~~]]	+	[[Category: Cameron SA]]
-	[[Category: Schramm~~, V L~~]]	+	[[Category: Schramm VL]]
-	[[Category: Wang, S]]	+	[[Category: Wang S]]
-	~~[[Category: Hydrolase-inhibitor complex~~]]	+

OCA at 10:51, 25 December 2019

OCA — Wed, 25 Dec 2019 10:51:23 GMT

←Older revision		Revision as of 10:51, 25 December 2019
Line 3:		Line 3:
	<StructureSection load='4ynb' size='340' side='right'caption='[[4ynb]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4ynb' size='340' side='right'caption='[[4ynb]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ynb]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YNB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YNB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ynb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_pylori_j99 Campylobacter pylori j99]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YNB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YNB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4EH:(3R,4S)-1-[(4-AMINO-5H-PYRROLO[3,2-D]PYRIMIDIN-7-YL)METHYL]-4-[(PYRAZIN-2-YLSULFANYL)METHYL]PYRROLIDIN-3-OL'>4EH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4EH:(3R,4S)-1-[(4-AMINO-5H-PYRROLO[3,2-D]PYRIMIDIN-7-YL)METHYL]-4-[(PYRAZIN-2-YLSULFANYL)METHYL]PYRROLIDIN-3-OL'>4EH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wkn\|4wkn]], [[4wko\|4wko]], [[4wkp\|4wkp]], [[4ffs\|4ffs]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wkn\|4wkn]], [[4wko\|4wko]], [[4wkp\|4wkp]], [[4ffs\|4ffs]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mtnN, mtn, jhp_0082 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85963 Campylobacter pylori J99])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ynb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ynb OCA], [http://pdbe.org/4ynb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ynb RCSB], [http://www.ebi.ac.uk/pdbsum/4ynb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ynb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ynb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ynb OCA], [http://pdbe.org/4ynb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ynb RCSB], [http://www.ebi.ac.uk/pdbsum/4ynb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ynb ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Campylobacter pylori j99]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Almo, S C]]		[[Category: Almo, S C]]

OCA at 06:40, 12 June 2019

OCA — Wed, 12 Jun 2019 06:40:25 GMT

←Older revision		Revision as of 06:40, 12 June 2019
Line 1:		Line 1:

	==Crystal structure of Helicobacter pylori 5'-methylthioadenosine/S-adenosyl homocysteine nucleosidase (MTAN) complexed with pyrazinylthio-DADMe-Immucillin-A==		==Crystal structure of Helicobacter pylori 5'-methylthioadenosine/S-adenosyl homocysteine nucleosidase (MTAN) complexed with pyrazinylthio-DADMe-Immucillin-A==
-	<StructureSection load='4ynb' size='340' side='right' caption='[[4ynb]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4ynb' size='340' side='right'caption='[[4ynb]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ynb]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YNB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YNB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ynb]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YNB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YNB FirstGlance]. <br>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Almo, S C]]		[[Category: Almo, S C]]
	[[Category: Cameron, S A]]		[[Category: Cameron, S A]]

OCA at 03:46, 6 September 2017

OCA — Wed, 06 Sep 2017 03:46:18 GMT

←Older revision		Revision as of 03:46, 6 September 2017
Line 1:		Line 1:
		+
	==Crystal structure of Helicobacter pylori 5'-methylthioadenosine/S-adenosyl homocysteine nucleosidase (MTAN) complexed with pyrazinylthio-DADMe-Immucillin-A==		==Crystal structure of Helicobacter pylori 5'-methylthioadenosine/S-adenosyl homocysteine nucleosidase (MTAN) complexed with pyrazinylthio-DADMe-Immucillin-A==
	<StructureSection load='4ynb' size='340' side='right' caption='[[4ynb]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4ynb' size='340' side='right' caption='[[4ynb]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4EH:(3R,4S)-1-[(4-AMINO-5H-PYRROLO[3,2-D]PYRIMIDIN-7-YL)METHYL]-4-[(PYRAZIN-2-YLSULFANYL)METHYL]PYRROLIDIN-3-OL'>4EH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4EH:(3R,4S)-1-[(4-AMINO-5H-PYRROLO[3,2-D]PYRIMIDIN-7-YL)METHYL]-4-[(PYRAZIN-2-YLSULFANYL)METHYL]PYRROLIDIN-3-OL'>4EH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wkn\|4wkn]], [[4wko\|4wko]], [[4wkp\|4wkp]], [[4ffs\|4ffs]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wkn\|4wkn]], [[4wko\|4wko]], [[4wkp\|4wkp]], [[4ffs\|4ffs]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ynb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ynb OCA], [http://pdbe.org/4ynb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ynb RCSB], [http://www.ebi.ac.uk/pdbsum/4ynb PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ynb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ynb OCA], [http://pdbe.org/4ynb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ynb RCSB], [http://www.ebi.ac.uk/pdbsum/4ynb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ynb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 04:39, 1 December 2015

OCA — Tue, 01 Dec 2015 04:39:44 GMT

←Older revision		Revision as of 04:39, 1 December 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of Helicobacter pylori 5'-methylthioadenosine/S-adenosyl homocysteine nucleosidase (MTAN) complexed with pyrazinylthio-DADMe-Immucillin-A==
		+	<StructureSection load='4ynb' size='340' side='right' caption='[[4ynb]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ynb]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YNB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YNB FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4EH:(3R,4S)-1-[(4-AMINO-5H-PYRROLO[3,2-D]PYRIMIDIN-7-YL)METHYL]-4-[(PYRAZIN-2-YLSULFANYL)METHYL]PYRROLIDIN-3-OL'>4EH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4wkn\|4wkn]], [[4wko\|4wko]], [[4wkp\|4wkp]], [[4ffs\|4ffs]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ynb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ynb OCA], [http://pdbe.org/4ynb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ynb RCSB], [http://www.ebi.ac.uk/pdbsum/4ynb PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MQMTN_HELPJ MQMTN_HELPJ]] Catalyzes the direct conversion of aminodeoxyfutalosine (AFL) into dehypoxanthine futalosine (DHFL) and adenine via the hydrolysis of the N-glycosidic bond; this reaction seems to represent an essential step in the menaquinone biosynthesis pathway in Helicobacter species. Also catalyzes the hydrolysis of 5'-methylthioadenosine (MTA) to adenine and 5'-methylthioribose. Can also probably use S-adenosylhomocysteine (SAH) as substrate, leading to adenine and S-ribosylhomocysteine. These other activities highlight the tremendous versatility of the enzyme, which also plays key roles in S-adenosylmethionine recycling and in the biosynthesis of the quorum-sensing molecule autoinducer-2.<ref>PMID:20954236</ref> <ref>PMID:22891633</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Helicobacter pylori is a Gram-negative bacterium that colonizes the gut of over 50% of the world's population. It is responsible for most peptic ulcers and is an important risk factor for gastric cancer. Antibiotic treatment for H. pylori infections is challenging as drug resistance has developed to antibiotics with traditional mechanisms of action. H. pylori uses an unusual pathway for menaquinone biosynthesis with 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) catalyzing an essential step. We validated MTAN as a target with a transition-state analogue of the enzyme [Wang, S.; Haapalainen, A. M.; Yan, F.; et al. Biochemistry 2012, 51, 6892-6894]. MTAN inhibitors will only be useful drug candidates if they can both include tight binding to the MTAN target and have the ability to penetrate the complex cell membrane found in Gram-negative H. pylori. Here we explore structural scaffolds for MTAN inhibition and for growth inhibition of cultured H. pylori. Sixteen analogues reported here are transition-state analogues of H. pylori MTAN with dissociation constants of 50 pM or below. Ten of these prevent growth of the H. pylori with IC90 values below 0.01 mug/mL. These remarkable compounds meet the criteria for potent inhibition and cell penetration. As a consequence, 10 new H. pylori antibiotic candidates are identified, all of which prevent H. pylori growth at concentrations 16-2000-fold lower than the five antibiotics, amoxicillin, metronidazole, levofloxacin, tetracyclin, and clarithromycin, commonly used to treat H. pylori infections. X-ray crystal structures of MTAN cocrystallized with several inhibitors show them to bind in the active site making interactions consistent with transition-state analogues.

-	~~The entry 4ynb is ON HOLD until Paper Publication~~	+	New Antibiotic Candidates against Helicobacter pylori.,Wang S, Cameron SA, Clinch K, Evans GB, Wu Z, Schramm VL, Tyler PC J Am Chem Soc. 2015 Nov 18;137(45):14275-80. doi: 10.1021/jacs.5b06110. Epub 2015, Nov 9. PMID:26494017<ref>PMID:26494017</ref>

-	~~Authors: Cameron~~, S.~~A., Wang,~~ S.~~, Almo, S.C., Schramm, V.L~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of Helicobacter pylori 5'-methylthioadenosine~~/~~S-adenosyl homocysteine nucleosidase (MTAN) complexed with pyrazinylthio-DADMe-Immucillin-A~~	+	<div class="pdbe-citations 4ynb" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
-	[[Category: ~~Almo~~, S.C]]	+	<references/>
-	[[Category: Schramm, V.L]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Almo, S C]]
		+	[[Category: Cameron, S A]]
		+	[[Category: Schramm, V L]]
	[[Category: Wang, S]]		[[Category: Wang, S]]
-	[[Category: ~~Cameron, S.A~~]]	+	[[Category: Hydrolase-inhibitor complex]]

OCA at 11:01, 22 April 2015

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-	The entry 4ynb is ON HOLD	+	The entry 4ynb is ON HOLD until Paper Publication

	Authors: Cameron, S.A., Wang, S., Almo, S.C., Schramm, V.L.		Authors: Cameron, S.A., Wang, S., Almo, S.C., Schramm, V.L.

OCA: Protected "4ynb" [edit=sysop:move=sysop]

OCA — Wed, 18 Mar 2015 10:54:02 GMT

Protected "4ynb" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:54, 18 March 2015

OCA: New page: '''Unreleased structure''' The entry 4ynb is ON HOLD Authors: Cameron, S.A., Wang, S., Almo, S.C., Schramm, V.L. Description: Crystal structure of Helicobacter pylori 5'-methylthioaden...

OCA — Wed, 18 Mar 2015 10:54:00 GMT

New page: '''Unreleased structure''' The entry 4ynb is ON HOLD Authors: Cameron, S.A., Wang, S., Almo, S.C., Schramm, V.L. Description: Crystal structure of Helicobacter pylori 5'-methylthioaden...

New page

'''Unreleased structure'''

The entry 4ynb is ON HOLD

Authors: Cameron, S.A., Wang, S., Almo, S.C., Schramm, V.L.

Description: Crystal structure of Helicobacter pylori 5'-methylthioadenosine/S-adenosyl homocysteine nucleosidase (MTAN) complexed with pyrazinylthio-DADMe-Immucillin-A
[[Category: Unreleased Structures]]
[[Category: Almo, S.C]]
[[Category: Schramm, V.L]]
[[Category: Wang, S]]
[[Category: Cameron, S.A]]