4yws - Revision history

OCA at 08:08, 27 September 2023

OCA — Wed, 27 Sep 2023 08:08:50 GMT

←Older revision		Revision as of 08:08, 27 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4yws]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chloroflexus_aurantiacus_J-10-fl Chloroflexus aurantiacus J-10-fl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YWS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4yws]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chloroflexus_aurantiacus_J-10-fl Chloroflexus aurantiacus J-10-fl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YWS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.45Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yws OCA], [https://pdbe.org/4yws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yws RCSB], [https://www.ebi.ac.uk/pdbsum/4yws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yws ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yws OCA], [https://pdbe.org/4yws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yws RCSB], [https://www.ebi.ac.uk/pdbsum/4yws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yws ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:29, 10 May 2023

OCA — Wed, 10 May 2023 07:29:31 GMT

←Older revision		Revision as of 07:29, 10 May 2023
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	<StructureSection load='4yws' size='340' side='right'caption='[[4yws]], [[Resolution\|resolution]] 2.45Å' scene=''>		<StructureSection load='4yws' size='340' side='right'caption='[[4yws]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4yws]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Chlaa Chlaa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YWS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4YWS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4yws]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chloroflexus_aurantiacus_J-10-fl Chloroflexus aurantiacus J-10-fl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YWS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4z17\|4z17]], [[4z1y\|4z1y]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yws OCA], [https://pdbe.org/4yws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yws RCSB], [https://www.ebi.ac.uk/pdbsum/4yws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yws ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4yws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yws OCA], [~~http~~://pdbe.org/4yws PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4yws RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4yws PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4yws ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ENO_CHLAA ENO_CHLAA]] Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.	+	[https://www.uniprot.org/uniprot/ENO_CHLAA ENO_CHLAA] Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 20:

	==See Also==		==See Also==
-	*[[Enolase\|Enolase]]	+	*[[Enolase 3D structures\|Enolase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chlaa~~]]	+	[[Category: Chloroflexus aurantiacus J-10-fl]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Phosphopyruvate hydratase]]~~	+	[[Category: Peters JW]]
-	[[Category: Peters~~, J W~~]]	+	[[Category: Zadvornyy OA]]
-	[[Category: Zadvornyy~~, O A]]~~	+
-	~~[[Category: Enolase]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Phylogeny]]~~	+
-	~~[[Category: Thermophilic origin]]~~	+
-	~~[[Category: Thermostability~~]]	+

OCA at 06:50, 19 June 2019

OCA — Wed, 19 Jun 2019 06:50:49 GMT

←Older revision		Revision as of 06:50, 19 June 2019
Line 1:		Line 1:

	==Thermostable enolase from Chloroflexus aurantiacus==		==Thermostable enolase from Chloroflexus aurantiacus==
-	<StructureSection load='4yws' size='340' side='right' caption='[[4yws]], [[Resolution\|resolution]] 2.45Å' scene=''>	+	<StructureSection load='4yws' size='340' side='right'caption='[[4yws]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4yws]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Chloroflexus_aurantiacus_(strain_atcc_29366_/_dsm_635_/_j-10-fl) Chloroflexus aurantiacus (strain atcc 29366 / dsm 635 / j-10-fl)~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YWS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4yws]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlaa Chlaa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YWS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4z17\|4z17]], [[4z1y\|4z1y]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4z17\|4z17]], [[4z1y\|4z1y]]</td></tr>
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 4yws" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4yws" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Enolase\|Enolase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chlaa]]
		+	[[Category: Large Structures]]
	[[Category: Phosphopyruvate hydratase]]		[[Category: Phosphopyruvate hydratase]]
	[[Category: Peters, J W]]		[[Category: Peters, J W]]

OCA at 13:48, 5 April 2017

OCA — Wed, 05 Apr 2017 13:48:47 GMT

←Older revision		Revision as of 13:48, 5 April 2017
Line 1:		Line 1:
		+
	==Thermostable enolase from Chloroflexus aurantiacus==		==Thermostable enolase from Chloroflexus aurantiacus==
	<StructureSection load='4yws' size='340' side='right' caption='[[4yws]], [[Resolution\|resolution]] 2.45Å' scene=''>		<StructureSection load='4yws' size='340' side='right' caption='[[4yws]], [[Resolution\|resolution]] 2.45Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4z17\|4z17]], [[4z1y\|4z1y]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4z17\|4z17]], [[4z1y\|4z1y]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yws OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4yws RCSB], [http://www.ebi.ac.uk/pdbsum/4yws PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yws OCA], [http://pdbe.org/4yws PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yws RCSB], [http://www.ebi.ac.uk/pdbsum/4yws PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4yws ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4yws" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:08, 1 July 2015

OCA — Wed, 01 Jul 2015 12:08:20 GMT

←Older revision		Revision as of 12:08, 1 July 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Thermostable enolase from Chloroflexus aurantiacus==
		+	<StructureSection load='4yws' size='340' side='right' caption='[[4yws]], [[Resolution\|resolution]] 2.45Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4yws]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chloroflexus_aurantiacus_(strain_atcc_29366_/_dsm_635_/_j-10-fl) Chloroflexus aurantiacus (strain atcc 29366 / dsm 635 / j-10-fl)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YWS FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4z17\|4z17]], [[4z1y\|4z1y]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yws OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4yws RCSB], [http://www.ebi.ac.uk/pdbsum/4yws PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ENO_CHLAA ENO_CHLAA]] Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during both glycolysis and gluconeogenesis, and is required by all three domains of life. Here, we report the purification and biochemical and structural characterization of enolase from Chloroflexus aurantiacus, a thermophilic anoxygenic phototroph affiliated with the green non-sulfur bacteria. The protein was purified as a homodimer with a subunit molecular weight of 46 kDa. The temperature optimum for enolase catalysis was 80 degrees C, close to the measured thermal stability of the protein which was determined to be 75 degrees C, while the pH optimum for enzyme activity was 6.5. The specific activities of purified enolase determined at 25 and 80 degrees C were 147 and 300 U mg(-1) of protein, respectively. K m values for the 2-phosphoglycerate/phosphoenolpyruvate reaction determined at 25 and 80 degrees C were 0.16 and 0.03 mM, respectively. The K m values for Mg(2+) binding at these temperatures were 2.5 and 1.9 mM, respectively. When compared to enolase from mesophiles, the biochemical and structural properties of enolase from C. aurantiacus are consistent with this being thermally adapted. These data are consistent with the results of our phylogenetic analysis of enolase, which reveal that enolase has a thermophilic origin.

-	~~The entry 4yws is ON HOLD until Paper Publication~~	+	Biochemical and Structural Characterization of Enolase from Chloroflexus aurantiacus: Evidence for a Thermophilic Origin.,Zadvornyy OA, Boyd ES, Posewitz MC, Zorin NA, Peters JW Front Bioeng Biotechnol. 2015 Jun 1;3:74. doi: 10.3389/fbioe.2015.00074., eCollection 2015. PMID:26082925<ref>PMID:26082925</ref>

-	~~Authors: Zadvornyy~~, O.A.~~, Peters, J.W~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Thermostable enolase from Chloroflexus aurantiacus~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: Peters, J.W]]	+	__TOC__
-	[[Category: Zadvornyy, O.A]]	+	</StructureSection>
		+	[[Category: Phosphopyruvate hydratase]]
		+	[[Category: Peters, J W]]
		+	[[Category: Zadvornyy, O A]]
		+	[[Category: Enolase]]
		+	[[Category: Lyase]]
		+	[[Category: Phylogeny]]
		+	[[Category: Thermophilic origin]]
		+	[[Category: Thermostability]]

OCA at 10:14, 20 May 2015

OCA — Wed, 20 May 2015 10:14:54 GMT

←Older revision		Revision as of 10:14, 20 May 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4yws is ON HOLD	+	The entry 4yws is ON HOLD until Paper Publication

	Authors: Zadvornyy, O.A., Peters, J.W.		Authors: Zadvornyy, O.A., Peters, J.W.

OCA: Protected "4yws" [edit=sysop:move=sysop]

OCA — Wed, 01 Apr 2015 11:59:00 GMT

Protected "4yws" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:59, 1 April 2015

OCA: New page: '''Unreleased structure''' The entry 4yws is ON HOLD Authors: Zadvornyy, O.A., Peters, J.W. Description: Thermostable enolase from Chloroflexus aurantiacus [[Category: Unreleased Struc...

OCA — Wed, 01 Apr 2015 11:58:59 GMT

New page: '''Unreleased structure''' The entry 4yws is ON HOLD Authors: Zadvornyy, O.A., Peters, J.W. Description: Thermostable enolase from Chloroflexus aurantiacus [[Category: Unreleased Struc...

New page

'''Unreleased structure'''

The entry 4yws is ON HOLD

Authors: Zadvornyy, O.A., Peters, J.W.

Description: Thermostable enolase from Chloroflexus aurantiacus
[[Category: Unreleased Structures]]
[[Category: Peters, J.W]]
[[Category: Zadvornyy, O.A]]