4z6w - Revision history

OCA at 08:13, 27 September 2023

OCA — Wed, 27 Sep 2023 08:13:13 GMT

←Older revision		Revision as of 08:13, 27 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4z6w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevibacterium_fuscum Brevibacterium fuscum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z6W FirstGlance]. <br>		<table><tr><td colspan='2'>[[4z6w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevibacterium_fuscum Brevibacterium fuscum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z6W FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4NC:4-NITROCATECHOL'>4NC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.57Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4NC:4-NITROCATECHOL'>4NC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z6w OCA], [https://pdbe.org/4z6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z6w RCSB], [https://www.ebi.ac.uk/pdbsum/4z6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z6w ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z6w OCA], [https://pdbe.org/4z6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z6w RCSB], [https://www.ebi.ac.uk/pdbsum/4z6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z6w ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:41, 10 May 2023

OCA — Wed, 10 May 2023 07:41:25 GMT

←Older revision		Revision as of 07:41, 10 May 2023
Line 3:		Line 3:
	<StructureSection load='4z6w' size='340' side='right'caption='[[4z6w]], [[Resolution\|resolution]] 1.57Å' scene=''>		<StructureSection load='4z6w' size='340' side='right'caption='[[4z6w]], [[Resolution\|resolution]] 1.57Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4z6w]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~'brevibacterium_fuscum' 'brevibacterium~~ fuscum']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z6W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4Z6W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4z6w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevibacterium_fuscum Brevibacterium fuscum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z6W FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4NC:4-NITROCATECHOL'>4NC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4NC:4-NITROCATECHOL'>4NC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4z6l\|4z6l]], [[4z6m\|4z6m]], [[4z6n\|4z6n]], [[4z6o\|4z6o]], [[4z6q\|4z6q]], [[4z6r\|4z6r]], [[4z6s\|4z6s]], [[4z6t\|4z6t]], [[4z6u\|4z6u]], [[4z6v\|4z6v]], [[4z6z\|4z6z]], [[4z6p\|4z6p]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z6w OCA], [https://pdbe.org/4z6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z6w RCSB], [https://www.ebi.ac.uk/pdbsum/4z6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z6w ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4z6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z6w OCA], [~~http~~://pdbe.org/4z6w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4z6w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4z6w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4z6w ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q45135_9MICO Q45135_9MICO]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	[[Category: Brevibacterium fuscum]]		[[Category: Brevibacterium fuscum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kovaleva~~, E G~~]]	+	[[Category: Kovaleva EG]]
-	[[Category: Lipscomb~~, J D]]~~	+	[[Category: Lipscomb JD]]
-	~~[[Category: 2-his-1-carboxylate facial triad]]~~	+
-	~~[[Category: Acid-base catalysis]]~~	+
-	~~[[Category: Dioxygenase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Oxygen activation~~]]	+

OCA at 10:55, 25 December 2019

OCA — Wed, 25 Dec 2019 10:55:32 GMT

←Older revision		Revision as of 10:55, 25 December 2019
Line 1:		Line 1:

	==Structure of H200N variant of Homoprotocatechuate 2,3-Dioxygenase from B.fuscum in complex with 4-nitrocatechol at 1.57 Ang resolution==		==Structure of H200N variant of Homoprotocatechuate 2,3-Dioxygenase from B.fuscum in complex with 4-nitrocatechol at 1.57 Ang resolution==
-	<StructureSection load='4z6w' size='340' side='right' caption='[[4z6w]], [[Resolution\|resolution]] 1.57Å' scene=''>	+	<StructureSection load='4z6w' size='340' side='right'caption='[[4z6w]], [[Resolution\|resolution]] 1.57Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4z6w]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/'brevibacterium_fuscum' 'brevibacterium fuscum']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z6W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z6W FirstGlance]. <br>		<table><tr><td colspan='2'>[[4z6w]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/'brevibacterium_fuscum' 'brevibacterium fuscum']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z6W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z6W FirstGlance]. <br>
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 4z6w" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4z6w" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dioxygenase 3D structures\|Dioxygenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Brevibacterium fuscum]]		[[Category: Brevibacterium fuscum]]
		+	[[Category: Large Structures]]
	[[Category: Kovaleva, E G]]		[[Category: Kovaleva, E G]]
	[[Category: Lipscomb, J D]]		[[Category: Lipscomb, J D]]

OCA at 16:03, 15 November 2017

OCA — Wed, 15 Nov 2017 16:03:13 GMT

←Older revision		Revision as of 16:03, 15 November 2017
Line 1:		Line 1:
		+
	==Structure of H200N variant of Homoprotocatechuate 2,3-Dioxygenase from B.fuscum in complex with 4-nitrocatechol at 1.57 Ang resolution==		==Structure of H200N variant of Homoprotocatechuate 2,3-Dioxygenase from B.fuscum in complex with 4-nitrocatechol at 1.57 Ang resolution==
	<StructureSection load='4z6w' size='340' side='right' caption='[[4z6w]], [[Resolution\|resolution]] 1.57Å' scene=''>		<StructureSection load='4z6w' size='340' side='right' caption='[[4z6w]], [[Resolution\|resolution]] 1.57Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4z6w]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z6W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z6W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4z6w]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/'brevibacterium_fuscum' 'brevibacterium fuscum']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z6W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z6W FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4NC:4-NITROCATECHOL'>4NC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4NC:4-NITROCATECHOL'>4NC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4z6l\|4z6l]], [[4z6m\|4z6m]], [[4z6n\|4z6n]], [[4z6o\|4z6o]], [[4z6q\|4z6q]], [[4z6r\|4z6r]], [[4z6s\|4z6s]], [[4z6t\|4z6t]], [[4z6u\|4z6u]], [[4z6v\|4z6v]], [[4z6z\|4z6z]], [[4z6p\|4z6p]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4z6l\|4z6l]], [[4z6m\|4z6m]], [[4z6n\|4z6n]], [[4z6o\|4z6o]], [[4z6q\|4z6q]], [[4z6r\|4z6r]], [[4z6s\|4z6s]], [[4z6t\|4z6t]], [[4z6u\|4z6u]], [[4z6v\|4z6v]], [[4z6z\|4z6z]], [[4z6p\|4z6p]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z6w OCA], [http://pdbe.org/4z6w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z6w RCSB], [http://www.ebi.ac.uk/pdbsum/4z6w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z6w OCA], [http://pdbe.org/4z6w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z6w RCSB], [http://www.ebi.ac.uk/pdbsum/4z6w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z6w ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Brevibacterium fuscum]]
	[[Category: Kovaleva, E G]]		[[Category: Kovaleva, E G]]
	[[Category: Lipscomb, J D]]		[[Category: Lipscomb, J D]]

OCA at 07:06, 30 September 2015

OCA — Wed, 30 Sep 2015 07:06:07 GMT

←Older revision		Revision as of 07:06, 30 September 2015
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4NC:4-NITROCATECHOL'>4NC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4NC:4-NITROCATECHOL'>4NC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4z6l\|4z6l]], [[4z6m\|4z6m]], [[4z6n\|4z6n]], [[4z6o\|4z6o]], [[4z6q\|4z6q]], [[4z6r\|4z6r]], [[4z6s\|4z6s]], [[4z6t\|4z6t]], [[4z6u\|4z6u]], [[4z6v\|4z6v]], [[4z6z\|4z6z]], [[4z6p\|4z6p]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4z6l\|4z6l]], [[4z6m\|4z6m]], [[4z6n\|4z6n]], [[4z6o\|4z6o]], [[4z6q\|4z6q]], [[4z6r\|4z6r]], [[4z6s\|4z6s]], [[4z6t\|4z6t]], [[4z6u\|4z6u]], [[4z6v\|4z6v]], [[4z6z\|4z6z]], [[4z6p\|4z6p]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z6w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4z6w RCSB], [http://www.ebi.ac.uk/pdbsum/4z6w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z6w OCA], [http://pdbe.org/4z6w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z6w RCSB], [http://www.ebi.ac.uk/pdbsum/4z6w PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4z6w" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:29, 26 August 2015

OCA — Wed, 26 Aug 2015 13:29:32 GMT

←Older revision		Revision as of 13:29, 26 August 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Structure of H200N variant of Homoprotocatechuate 2,3-Dioxygenase from B.fuscum in complex with 4-nitrocatechol at 1.57 Ang resolution==
		+	<StructureSection load='4z6w' size='340' side='right' caption='[[4z6w]], [[Resolution\|resolution]] 1.57Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4z6w]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z6W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z6W FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4NC:4-NITROCATECHOL'>4NC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4z6l\|4z6l]], [[4z6m\|4z6m]], [[4z6n\|4z6n]], [[4z6o\|4z6o]], [[4z6q\|4z6q]], [[4z6r\|4z6r]], [[4z6s\|4z6s]], [[4z6t\|4z6t]], [[4z6u\|4z6u]], [[4z6v\|4z6v]], [[4z6z\|4z6z]], [[4z6p\|4z6p]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z6w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4z6w RCSB], [http://www.ebi.ac.uk/pdbsum/4z6w PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Kinetic and spectroscopic studies have shown that the conserved active site residue His200 of the extradiol ring-cleaving homoprotocatechuate 2,3-dioxygenase (FeHPCD) from Brevibacterium fuscum is critical for efficient catalysis. The roles played by this residue are probed here by analysis of the steady-state kinetics, pH dependence, and X-ray crystal structures of the FeHPCD position 200 variants His200Asn, His200Gln, and His200Glu alone and in complex with three catecholic substrates (homoprotocatechuate, 4-sulfonylcatechol, and 4-nitrocatechol) possessing substituents with different inductive capacity. Structures determined at 1.35-1.75 A resolution show that there is essentially no change in overall active site architecture or substrate binding mode for these variants when compared to the structures of the wild-type enzyme and its analogous complexes. This shows that the maximal 50-fold decrease in kcat for ring cleavage, the dramatic changes in pH dependence, and the switch from ring cleavage to ring oxidation of 4-nitrocatechol by the FeHPCD variants can be attributed specifically to the properties of the altered second-sphere residue and the substrate. The results suggest that proton transfer is necessary for catalysis, and that it occurs most efficiently when the substrate provides the proton and His200 serves as a catalyst. However, in the absence of an available substrate proton, a defined proton-transfer pathway in the protein can be utilized. Changes in the steric bulk and charge of the residue at position 200 appear to be capable of altering the rate-limiting step in catalysis and, perhaps, the nature of the reactive species.

-	~~The entry 4z6w is ON HOLD until Paper Publication~~	+	Structural Basis for Substrate and Oxygen Activation in Homoprotocatechuate 2,3-Dioxygenase: Roles of Conserved Active Site Histidine 200.,Kovaleva EG, Rogers MS, Lipscomb JD Biochemistry. 2015 Aug 19. PMID:26267790<ref>PMID:26267790</ref>

-	~~Authors~~: Kovaleva, E.G., Lipscomb, J.D.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~:	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: ~~Lipscomb, J.D~~]]	+	__TOC__
-	[[Category: ~~Kovaleva, E.G~~]]	+	</StructureSection>
		+	[[Category: Kovaleva, E G]]
		+	[[Category: Lipscomb, J D]]
		+	[[Category: 2-his-1-carboxylate facial triad]]
		+	[[Category: Acid-base catalysis]]
		+	[[Category: Dioxygenase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Oxygen activation]]

OCA at 11:26, 6 May 2015

OCA — Wed, 06 May 2015 11:26:57 GMT

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-	The entry 4z6w is ON HOLD	+	The entry 4z6w is ON HOLD until Paper Publication

	Authors: Kovaleva, E.G., Lipscomb, J.D.		Authors: Kovaleva, E.G., Lipscomb, J.D.

OCA: Protected "4z6w" [edit=sysop:move=sysop]

OCA — Wed, 15 Apr 2015 11:44:06 GMT

Protected "4z6w" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:44, 15 April 2015

OCA: New page: '''Unreleased structure''' The entry 4z6w is ON HOLD Authors: Kovaleva, E.G., Lipscomb, J.D. Description: Category: Unreleased Structures Category: Lipscomb, J.D [[Category: K...

OCA — Wed, 15 Apr 2015 11:44:05 GMT

New page: '''Unreleased structure''' The entry 4z6w is ON HOLD Authors: Kovaleva, E.G., Lipscomb, J.D. Description: Category: Unreleased Structures Category: Lipscomb, J.D [[Category: K...

New page

'''Unreleased structure'''

The entry 4z6w is ON HOLD

Authors: Kovaleva, E.G., Lipscomb, J.D.

Description:
[[Category: Unreleased Structures]]
[[Category: Lipscomb, J.D]]
[[Category: Kovaleva, E.G]]