4zgo - Revision history

OCA at 07:20, 1 May 2024

OCA — Wed, 01 May 2024 07:20:51 GMT

←Older revision		Revision as of 07:20, 1 May 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4zgo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZGO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZGO FirstGlance]. <br>		<table><tr><td colspan='2'>[[4zgo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZGO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZGO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zgo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zgo OCA], [https://pdbe.org/4zgo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zgo RCSB], [https://www.ebi.ac.uk/pdbsum/4zgo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zgo ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.063Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zgo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zgo OCA], [https://pdbe.org/4zgo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zgo RCSB], [https://www.ebi.ac.uk/pdbsum/4zgo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zgo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/CD123_SCHPO CD123_SCHPO] Regulates the cell cycle in a nutrient dependent manner.		[https://www.uniprot.org/uniprot/CD123_SCHPO CD123_SCHPO] Regulates the cell cycle in a nutrient dependent manner.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Eukaryotic initiation factor 2 (eIF2), a heterotrimeric guanosine triphosphatase, has a central role in protein biosynthesis by supplying methionylated initiator tRNA to the ribosomal translation initiation complex and by serving as a target for translational control in response to stress. Recent work identified a novel step indispensable for eIF2 function: assembly of eIF2 from its three subunits by the cell proliferation protein Cdc123. We report the first crystal structure of a Cdc123 representative, that from Schizosaccharomyces pombe, both isolated and bound to domain III of Saccharomyces cerevisiae eIF2gamma. The structures show that Cdc123 resembles enzymes of the ATP-grasp family. Indeed, Cdc123 binds ATP-Mg(2+), and conserved residues contacting ATP-Mg(2+) are essential for Cdc123 to support eIF2 assembly and cell viability. A docking of eIF2alphagamma onto Cdc123, combined with genetic and biochemical experiments, allows us to propose a model explaining how Cdc123 participates in the biogenesis of eIF2 through facilitating assembly of eIF2gamma to eIF2alpha.
-
-	Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features.,Panvert M, Dubiez E, Arnold L, Perez J, Mechulam Y, Seufert W, Schmitt E Structure. 2015 Sep 1;23(9):1596-608. doi: 10.1016/j.str.2015.06.014. Epub 2015, Jul 23. PMID:26211610<ref>PMID:26211610</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4zgo" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:27, 18 May 2023

OCA — Thu, 18 May 2023 07:27:06 GMT

←Older revision		Revision as of 07:27, 18 May 2023
Line 3:		Line 3:
	<StructureSection load='4zgo' size='340' side='right'caption='[[4zgo]], [[Resolution\|resolution]] 2.06Å' scene=''>		<StructureSection load='4zgo' size='340' side='right'caption='[[4zgo]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zgo]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Cbs_356 Cbs 356~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZGO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ZGO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zgo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZGO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZGO FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cdc123, SPAP27G11.03 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zgo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zgo OCA], [https://pdbe.org/4zgo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zgo RCSB], [https://www.ebi.ac.uk/pdbsum/4zgo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zgo ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4zgo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zgo OCA], [~~http~~://pdbe.org/4zgo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4zgo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4zgo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4zgo ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CD123_SCHPO CD123_SCHPO]] Regulates the cell cycle in a nutrient dependent manner.	+	[https://www.uniprot.org/uniprot/CD123_SCHPO CD123_SCHPO] Regulates the cell cycle in a nutrient dependent manner.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cbs 356]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Arnold, L]]	+	[[Category: Schizosaccharomyces pombe]]
-	[[Category: Dubiez, E]]	+	[[Category: Arnold L]]
-	[[Category: Mechulam, Y]]	+	[[Category: Dubiez E]]
-	[[Category: Panvert, M]]	+	[[Category: Mechulam Y]]
-	[[Category: Perez, J]]	+	[[Category: Panvert M]]
-	[[Category: Schmitt, E]]	+	[[Category: Perez J]]
-	[[Category: Seufert, W]]	+	[[Category: Schmitt E]]
-	~~[[Category: Atp-grap fold]]~~	+	[[Category: Seufert W]]
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Eif2 assembly~~]]	+

OCA at 06:47, 19 June 2019

OCA — Wed, 19 Jun 2019 06:47:14 GMT

←Older revision		Revision as of 06:47, 19 June 2019
Line 1:		Line 1:
		+
	==Structure of C-terminally truncated Cdc123 from Schizosaccharomyces pombe==		==Structure of C-terminally truncated Cdc123 from Schizosaccharomyces pombe==
-	<StructureSection load='4zgo' size='340' side='right' caption='[[4zgo]], [[Resolution\|resolution]] 2.06Å' scene=''>	+	<StructureSection load='4zgo' size='340' side='right'caption='[[4zgo]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zgo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZGO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZGO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zgo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_356 Cbs 356]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZGO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZGO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zgo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zgo OCA], [http://pdbe.org/4zgo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zgo RCSB], [http://www.ebi.ac.uk/pdbsum/4zgo PDBsum]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cdc123, SPAP27G11.03 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zgo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zgo OCA], [http://pdbe.org/4zgo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zgo RCSB], [http://www.ebi.ac.uk/pdbsum/4zgo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zgo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cbs 356]]
		+	[[Category: Large Structures]]
	[[Category: Arnold, L]]		[[Category: Arnold, L]]
	[[Category: Dubiez, E]]		[[Category: Dubiez, E]]

OCA at 14:31, 30 September 2015

OCA — Wed, 30 Sep 2015 14:31:05 GMT

←Older revision		Revision as of 14:31, 30 September 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Structure of C-terminally truncated Cdc123 from Schizosaccharomyces pombe==
		+	<StructureSection load='4zgo' size='340' side='right' caption='[[4zgo]], [[Resolution\|resolution]] 2.06Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4zgo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZGO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZGO FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zgo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zgo OCA], [http://pdbe.org/4zgo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zgo RCSB], [http://www.ebi.ac.uk/pdbsum/4zgo PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CD123_SCHPO CD123_SCHPO]] Regulates the cell cycle in a nutrient dependent manner.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Eukaryotic initiation factor 2 (eIF2), a heterotrimeric guanosine triphosphatase, has a central role in protein biosynthesis by supplying methionylated initiator tRNA to the ribosomal translation initiation complex and by serving as a target for translational control in response to stress. Recent work identified a novel step indispensable for eIF2 function: assembly of eIF2 from its three subunits by the cell proliferation protein Cdc123. We report the first crystal structure of a Cdc123 representative, that from Schizosaccharomyces pombe, both isolated and bound to domain III of Saccharomyces cerevisiae eIF2gamma. The structures show that Cdc123 resembles enzymes of the ATP-grasp family. Indeed, Cdc123 binds ATP-Mg(2+), and conserved residues contacting ATP-Mg(2+) are essential for Cdc123 to support eIF2 assembly and cell viability. A docking of eIF2alphagamma onto Cdc123, combined with genetic and biochemical experiments, allows us to propose a model explaining how Cdc123 participates in the biogenesis of eIF2 through facilitating assembly of eIF2gamma to eIF2alpha.

-	~~The entry 4zgo is ON HOLD~~	+	Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features.,Panvert M, Dubiez E, Arnold L, Perez J, Mechulam Y, Seufert W, Schmitt E Structure. 2015 Sep 1;23(9):1596-608. doi: 10.1016/j.str.2015.06.014. Epub 2015, Jul 23. PMID:26211610<ref>PMID:26211610</ref>

-	~~Authors: Panvert~~, M.~~, Dubiez, E~~.~~, Arnold, L., Perez, J., Seufert, W., Mechulam, Y., Schmitt, E~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~:	+	<div class="pdbe-citations 4zgo" style="background-color:#fffaf0;"></div>
-	~~[[Category: Unreleased Structures]]~~	+	== References ==
-	[[Category: ~~Schmitt~~, E]]	+	<references/>
-	[[Category: ~~Perez~~, J]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Arnold, L]]
		+	[[Category: Dubiez, E]]
	[[Category: Mechulam, Y]]		[[Category: Mechulam, Y]]
-	[[Category: Dubiez, E]]
	[[Category: Panvert, M]]		[[Category: Panvert, M]]
-	[[Category: ~~Arnold~~, L]]	+	[[Category: Perez, J]]
		+	[[Category: Schmitt, E]]
	[[Category: Seufert, W]]		[[Category: Seufert, W]]
		+	[[Category: Atp-grap fold]]
		+	[[Category: Cell cycle]]
		+	[[Category: Eif2 assembly]]

OCA: Protected "4zgo" [edit=sysop:move=sysop]

OCA — Thu, 30 Apr 2015 10:22:12 GMT

Protected "4zgo" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:22, 30 April 2015

OCA: New page: '''Unreleased structure''' The entry 4zgo is ON HOLD Authors: Panvert, M., Dubiez, E., Arnold, L., Perez, J., Seufert, W., Mechulam, Y., Schmitt, E. Description: [[Category: Unrelease...

OCA — Thu, 30 Apr 2015 10:22:10 GMT

New page: '''Unreleased structure''' The entry 4zgo is ON HOLD Authors: Panvert, M., Dubiez, E., Arnold, L., Perez, J., Seufert, W., Mechulam, Y., Schmitt, E. Description: [[Category: Unrelease...

New page

'''Unreleased structure'''

The entry 4zgo is ON HOLD

Authors: Panvert, M., Dubiez, E., Arnold, L., Perez, J., Seufert, W., Mechulam, Y., Schmitt, E.

Description:
[[Category: Unreleased Structures]]
[[Category: Schmitt, E]]
[[Category: Perez, J]]
[[Category: Mechulam, Y]]
[[Category: Dubiez, E]]
[[Category: Panvert, M]]
[[Category: Arnold, L]]
[[Category: Seufert, W]]