4zjq - Revision history

OCA at 11:00, 10 January 2024

OCA — Wed, 10 Jan 2024 11:00:28 GMT

←Older revision		Revision as of 11:00, 10 January 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4zjq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZJQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4zjq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZJQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ERY:ERYTHROMYCIN+A'>ERY</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.592Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ERY:ERYTHROMYCIN+A'>ERY</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zjq OCA], [https://pdbe.org/4zjq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zjq RCSB], [https://www.ebi.ac.uk/pdbsum/4zjq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zjq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zjq OCA], [https://pdbe.org/4zjq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zjq RCSB], [https://www.ebi.ac.uk/pdbsum/4zjq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zjq ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:30, 18 May 2023

OCA — Thu, 18 May 2023 07:30:42 GMT

←Older revision		Revision as of 07:30, 18 May 2023
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	<StructureSection load='4zjq' size='340' side='right'caption='[[4zjq]], [[Resolution\|resolution]] 3.59Å' scene=''>		<StructureSection load='4zjq' size='340' side='right'caption='[[4zjq]], [[Resolution\|resolution]] 3.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zjq]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZJQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ZJQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zjq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZJQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ERY:ERYTHROMYCIN+A'>ERY</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ERY:ERYTHROMYCIN+A'>ERY</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4zjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zjq OCA], [~~http~~://pdbe.org/4zjq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4zjq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4zjq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4zjq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zjq OCA], [https://pdbe.org/4zjq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zjq RCSB], [https://www.ebi.ac.uk/pdbsum/4zjq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zjq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ACRB_ECOLI ACRB_ECOLI]] AcrAB is a drug efflux protein with a broad substrate specificity.<ref>PMID:16915237</ref> <ref>PMID:16946072</ref> <ref>PMID:17194213</ref>	+	[https://www.uniprot.org/uniprot/ACRB_ECOLI ACRB_ECOLI] AcrAB is a drug efflux protein with a broad substrate specificity.<ref>PMID:16915237</ref> <ref>PMID:16946072</ref> <ref>PMID:17194213</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ababou, A]]	+	[[Category: Ababou A]]
-	[[Category: Koronakis, V]]	+	[[Category: Koronakis V]]
-	~~[[Category: Transport protein~~]]	+

OCA at 07:04, 10 July 2019

OCA — Wed, 10 Jul 2019 07:04:11 GMT

←Older revision		Revision as of 07:04, 10 July 2019
Line 1:		Line 1:

	==Crystal structure of AcrB deletion mutant in complex with antibiotic in P21 space group==		==Crystal structure of AcrB deletion mutant in complex with antibiotic in P21 space group==
-	<StructureSection load='4zjq' size='340' side='right' caption='[[4zjq]], [[Resolution\|resolution]] 3.59Å' scene=''>	+	<StructureSection load='4zjq' size='340' side='right'caption='[[4zjq]], [[Resolution\|resolution]] 3.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4zjq]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZJQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4zjq]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZJQ FirstGlance]. <br>
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Ababou, A]]		[[Category: Ababou, A]]
	[[Category: Koronakis, V]]		[[Category: Koronakis, V]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 15:33, 27 July 2016

OCA — Wed, 27 Jul 2016 15:33:11 GMT

←Older revision		Revision as of 15:33, 27 July 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 4zjq is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of AcrB deletion mutant in complex with antibiotic in P21 space group==
		+	<StructureSection load='4zjq' size='340' side='right' caption='[[4zjq]], [[Resolution\|resolution]] 3.59Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4zjq]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZJQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ERY:ERYTHROMYCIN+A'>ERY</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zjq OCA], [http://pdbe.org/4zjq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zjq RCSB], [http://www.ebi.ac.uk/pdbsum/4zjq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zjq ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ACRB_ECOLI ACRB_ECOLI]] AcrAB is a drug efflux protein with a broad substrate specificity.<ref>PMID:16915237</ref> <ref>PMID:16946072</ref> <ref>PMID:17194213</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Gram-negative bacteria such as E. coli use tripartite efflux pumps such as AcrAB-TolC to expel antibiotics and noxious compounds. A key feature of the inner membrane transporter component, AcrB, is a short stretch of residues known as the gate/switch loop that divides the proximal and distal substrate binding pockets. Amino acid substitutions of the gate loop are known to decrease antibiotic resistance conferred by AcrB. Here we present two new AcrB gate loop variants, the first stripped of its bulky side chains, and a second in which the gate loop is removed entirely. By determining the crystal structures of the variant AcrB proteins in the presence and absence of erythromycin and assessing their ability to confer erythromycin tolerance, we demonstrate that the gate loop is important for AcrB export activity but is not required for erythromycin binding.

-	~~Authors: Ababou~~, A., Koronakis, V.	+	Structures of Gate Loop Variants of the AcrB Drug Efflux Pump Bound by Erythromycin Substrate.,Ababou A, Koronakis V PLoS One. 2016 Jul 12;11(7):e0159154. doi: 10.1371/journal.pone.0159154., eCollection 2016. PMID:27403665<ref>PMID:27403665</ref>

-	~~Description: Crystal structure~~ of ~~AcrB deletion mutant in complex with antibiotic in P21 space group~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Koronakis, V]]~~	+	<div class="pdbe-citations 4zjq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Ababou, A]]		[[Category: Ababou, A]]
		+	[[Category: Koronakis, V]]
		+	[[Category: Transport protein]]

OCA at 20:27, 10 May 2016

OCA — Tue, 10 May 2016 20:27:50 GMT

←Older revision		Revision as of 20:27, 10 May 2016
Line 3:		Line 3:
	The entry 4zjq is ON HOLD until Paper Publication		The entry 4zjq is ON HOLD until Paper Publication

-	Authors: Ababou, A~~., Hughes, C~~., Koronakis, V.	+	Authors: Ababou, A., Koronakis, V.

	Description: Crystal structure of AcrB deletion mutant in complex with antibiotic in P21 space group		Description: Crystal structure of AcrB deletion mutant in complex with antibiotic in P21 space group
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Koronakis, V]]		[[Category: Koronakis, V]]
-	[[Category: Hughes, C]]
	[[Category: Ababou, A]]		[[Category: Ababou, A]]

OCA at 00:03, 1 December 2015

OCA — Tue, 01 Dec 2015 00:03:21 GMT

←Older revision		Revision as of 00:03, 1 December 2015
Line 7:		Line 7:
	Description: Crystal structure of AcrB deletion mutant in complex with antibiotic in P21 space group		Description: Crystal structure of AcrB deletion mutant in complex with antibiotic in P21 space group
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Ababou, A]]
	[[Category: Koronakis, V]]		[[Category: Koronakis, V]]
	[[Category: Hughes, C]]		[[Category: Hughes, C]]
		+	[[Category: Ababou, A]]

OCA at 11:02, 13 May 2015

OCA — Wed, 13 May 2015 11:02:31 GMT

←Older revision		Revision as of 11:02, 13 May 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4zjq is ON HOLD	+	The entry 4zjq is ON HOLD until Paper Publication

	Authors: Ababou, A., Hughes, C., Koronakis, V.		Authors: Ababou, A., Hughes, C., Koronakis, V.

OCA: Protected "4zjq" [edit=sysop:move=sysop]

OCA — Wed, 06 May 2015 11:50:34 GMT

Protected "4zjq" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:50, 6 May 2015

OCA: New page: '''Unreleased structure''' The entry 4zjq is ON HOLD Authors: Ababou, A., Hughes, C., Koronakis, V. Description: Crystal structure of AcrB deletion mutant in complex with antibiotic in...

OCA — Wed, 06 May 2015 11:50:33 GMT

New page: '''Unreleased structure''' The entry 4zjq is ON HOLD Authors: Ababou, A., Hughes, C., Koronakis, V. Description: Crystal structure of AcrB deletion mutant in complex with antibiotic in...

New page

'''Unreleased structure'''

The entry 4zjq is ON HOLD

Authors: Ababou, A., Hughes, C., Koronakis, V.

Description: Crystal structure of AcrB deletion mutant in complex with antibiotic in P21 space group
[[Category: Unreleased Structures]]
[[Category: Ababou, A]]
[[Category: Koronakis, V]]
[[Category: Hughes, C]]