4zlf - Revision history

OCA at 09:05, 20 March 2024

OCA — Wed, 20 Mar 2024 09:05:00 GMT

←Older revision		Revision as of 09:05, 20 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4zlf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharophagus_degradans_2-40 Saccharophagus degradans 2-40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZLF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZLF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4zlf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharophagus_degradans_2-40 Saccharophagus degradans 2-40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZLF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZLF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CEZ:4-O-BETA-D-GLUCOPYRANOSYL-D-GLUCONIC+ACID'>CEZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CEZ:4-O-BETA-D-GLUCOPYRANOSYL-D-GLUCONIC+ACID'>CEZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zlf OCA], [https://pdbe.org/4zlf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zlf RCSB], [https://www.ebi.ac.uk/pdbsum/4zlf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zlf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zlf OCA], [https://pdbe.org/4zlf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zlf RCSB], [https://www.ebi.ac.uk/pdbsum/4zlf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zlf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q21MB1_SACD2 Q21MB1_SACD2]		[https://www.uniprot.org/uniprot/Q21MB1_SACD2 Q21MB1_SACD2]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The microbial oxidative cellulose degradation system is attracting significant research attention after the recent discovery of lytic polysaccharide mono-oxygenases. A primary product of the oxidative and hydrolytic cellulose degradation system is cellobionic acid (CbA), the aldonic acid form of cellobiose. We previously demonstrated that the intracellular enzyme belonging to glycoside hydrolase (GH) family 94 from cellulolytic fungus and bacterium is cellobionic acid phosphorylase (CBAP), which catalyzes reversible phosphorolysis of CbA into glucose 1-phosphate and gluconic acid (GlcA). In this report we describe the biochemical characterization and the three-dimensional structure of CBAP from the marine cellulolytic bacterium Saccharophagus degradans. Structures of ligand-free and complex forms with CbA, GlcA, and a synthetic disaccharide product from glucuronic acid (GlcUA) were determined at resolutions of up to 1.6 A. The active site is located near the dimer interface. At subsite +1, the carboxylate group of GlcA and CbA is recognized by Arg-609 and Lys-613. Additionally, one residue from the neighboring protomer (Gln-190) is involved in the carboxylate recognition of GlcA. A mutational analysis indicated that these residues are critical for the binding and catalysis of the aldonic and uronic acid acceptors GlcA and GlcUA. Structural and sequence comparisons with other GH94 phosphorylases revealed that CBAPs have a unique subsite +1 with a distinct amino acid residue conservation pattern at this site. This study provides molecular insight into the energetically efficient metabolic pathway of oxidized sugars that links the oxidative cellulolytic pathway to the glycolytic and pentose phosphate pathways in cellulolytic microbes.
-
-	Crystal structure and substrate recognition of cellobionic acid phosphorylase playing a key role in oxidative cellulose degradation by microbes.,Nam YW, Nihira T, Arakawa T, Saito Y, Kitaoka M, Nakai H, Fushinobu S J Biol Chem. 2015 Jun 3. pii: jbc.M115.664664. PMID:26041776<ref>PMID:26041776</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4zlf" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:32, 18 May 2023

OCA — Thu, 18 May 2023 07:32:23 GMT

←Older revision		Revision as of 07:32, 18 May 2023
Line 3:		Line 3:
	<StructureSection load='4zlf' size='340' side='right'caption='[[4zlf]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='4zlf' size='340' side='right'caption='[[4zlf]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zlf]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Microbulbifer_degradans_2~~-40 ~~Microbulbifer~~ degradans 2-40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZLF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ZLF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zlf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharophagus_degradans_2-40 Saccharophagus degradans 2-40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZLF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZLF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CEZ:4-O-BETA-D-GLUCOPYRANOSYL-D-GLUCONIC+ACID'>CEZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CEZ:4-O-BETA-D-GLUCOPYRANOSYL-D-GLUCONIC+ACID'>CEZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4zle\|4zle]], [[4zlg\|4zlg]], [[4zli\|4zli]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zlf OCA], [https://pdbe.org/4zlf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zlf RCSB], [https://www.ebi.ac.uk/pdbsum/4zlf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zlf ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cep94B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203122 Microbulbifer degradans 2-40])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellobionic_acid_phosphorylase Cellobionic acid phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.321 2.4.1.321] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4zlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zlf OCA], [~~http~~://pdbe.org/4zlf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4zlf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4zlf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4zlf ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q21MB1_SACD2 Q21MB1_SACD2]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cellobionic acid phosphorylase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Microbulbifer~~ degradans 2-40]]	+	[[Category: Saccharophagus degradans 2-40]]
-	[[Category: Arakawa, T]]	+	[[Category: Arakawa T]]
-	[[Category: Fushinobu, S]]	+	[[Category: Fushinobu S]]
-	[[Category: Nam~~, Y W]]~~	+	[[Category: Nam YW]]
-	~~[[Category: Glycoside hydrolase family 94]]~~	+
-	~~[[Category: Intracellular enzyme]]~~	+
-	~~[[Category: Oxidative cellulose degradation system]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:57, 26 June 2019

OCA — Wed, 26 Jun 2019 07:57:29 GMT

←Older revision		Revision as of 07:57, 26 June 2019
Line 1:		Line 1:

	==Cellobionic acid phosphorylase - cellobionic acid complex==		==Cellobionic acid phosphorylase - cellobionic acid complex==
-	<StructureSection load='4zlf' size='340' side='right' caption='[[4zlf]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='4zlf' size='340' side='right'caption='[[4zlf]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zlf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZLF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZLF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zlf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Microbulbifer_degradans_2-40 Microbulbifer degradans 2-40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZLF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZLF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CEZ:4-O-BETA-D-GLUCOPYRANOSYL-D-GLUCONIC+ACID'>CEZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CEZ:4-O-BETA-D-GLUCOPYRANOSYL-D-GLUCONIC+ACID'>CEZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4zle\|4zle]], [[4zlg\|4zlg]], [[4zli\|4zli]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4zle\|4zle]], [[4zlg\|4zlg]], [[4zli\|4zli]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cep94B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203122 Microbulbifer degradans 2-40])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellobionic_acid_phosphorylase Cellobionic acid phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.321 2.4.1.321] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellobionic_acid_phosphorylase Cellobionic acid phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.321 2.4.1.321] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zlf OCA], [http://pdbe.org/4zlf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zlf RCSB], [http://www.ebi.ac.uk/pdbsum/4zlf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zlf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zlf OCA], [http://pdbe.org/4zlf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zlf RCSB], [http://www.ebi.ac.uk/pdbsum/4zlf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zlf ProSAT]</span></td></tr>
Line 23:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Cellobionic acid phosphorylase]]		[[Category: Cellobionic acid phosphorylase]]
		+	[[Category: Large Structures]]
		+	[[Category: Microbulbifer degradans 2-40]]
	[[Category: Arakawa, T]]		[[Category: Arakawa, T]]
	[[Category: Fushinobu, S]]		[[Category: Fushinobu, S]]

OCA at 13:20, 10 May 2017

OCA — Wed, 10 May 2017 13:20:38 GMT

←Older revision		Revision as of 13:20, 10 May 2017
Line 1:		Line 1:
		+
	==Cellobionic acid phosphorylase - cellobionic acid complex==		==Cellobionic acid phosphorylase - cellobionic acid complex==
	<StructureSection load='4zlf' size='340' side='right' caption='[[4zlf]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='4zlf' size='340' side='right' caption='[[4zlf]], [[Resolution\|resolution]] 1.60Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4zle\|4zle]], [[4zlg\|4zlg]], [[4zli\|4zli]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4zle\|4zle]], [[4zlg\|4zlg]], [[4zli\|4zli]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellobionic_acid_phosphorylase Cellobionic acid phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.321 2.4.1.321] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellobionic_acid_phosphorylase Cellobionic acid phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.321 2.4.1.321] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zlf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4zlf RCSB], [http://www.ebi.ac.uk/pdbsum/4zlf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zlf OCA], [http://pdbe.org/4zlf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zlf RCSB], [http://www.ebi.ac.uk/pdbsum/4zlf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zlf ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4zlf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:29, 17 June 2015

OCA — Wed, 17 Jun 2015 08:29:29 GMT

←Older revision		Revision as of 08:29, 17 June 2015
Line 10:		Line 10:
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
-	A ~~novel phosphorylase was characterized as new member~~ of glycoside hydrolase family 94 from ~~the~~ cellulolytic ~~bacterium Xanthomonas campestris and the~~ fungus ~~Neurospora crassa. The enzyme catalyzed~~ reversible phosphorolysis of ~~cellobionic acid. We propose 4-O-beta-D-glucopyranosyl-D~~-gluconic acid~~: phosphate alpha-D-glucosyltransferase as~~ the ~~systematic name~~ and ~~cellobionic acid phosphorylase as~~ the ~~short names for the novel enzyme. Several cellulolytic fungi~~ of the ~~phylum Ascomycota also possess homologous proteins~~. We, ~~therefore~~, ~~suggest that the enzyme plays~~ a ~~crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic~~ product is ~~converted into alpha-D-glucose~~ 1-~~phosphate~~ and D-~~gluconic~~ acid ~~to enter glycolysis~~ and the pentose phosphate ~~pathway, respectively~~.	+	The microbial oxidative cellulose degradation system is attracting significant research attention after the recent discovery of lytic polysaccharide mono-oxygenases. A primary product of the oxidative and hydrolytic cellulose degradation system is cellobionic acid (CbA), the aldonic acid form of cellobiose. We previously demonstrated that the intracellular enzyme belonging to glycoside hydrolase (GH) family 94 from cellulolytic fungus and bacterium is cellobionic acid phosphorylase (CBAP), which catalyzes reversible phosphorolysis of CbA into glucose 1-phosphate and gluconic acid (GlcA). In this report we describe the biochemical characterization and the three-dimensional structure of CBAP from the marine cellulolytic bacterium Saccharophagus degradans. Structures of ligand-free and complex forms with CbA, GlcA, and a synthetic disaccharide product from glucuronic acid (GlcUA) were determined at resolutions of up to 1.6 A. The active site is located near the dimer interface. At subsite +1, the carboxylate group of GlcA and CbA is recognized by Arg-609 and Lys-613. Additionally, one residue from the neighboring protomer (Gln-190) is involved in the carboxylate recognition of GlcA. A mutational analysis indicated that these residues are critical for the binding and catalysis of the aldonic and uronic acid acceptors GlcA and GlcUA. Structural and sequence comparisons with other GH94 phosphorylases revealed that CBAPs have a unique subsite +1 with a distinct amino acid residue conservation pattern at this site. This study provides molecular insight into the energetically efficient metabolic pathway of oxidized sugars that links the oxidative cellulolytic pathway to the glycolytic and pentose phosphate pathways in cellulolytic microbes.

-	~~Discovery~~ of cellobionic acid phosphorylase in ~~cellulolytic bacteria and fungi~~.,Nihira T, Saito Y~~, Nishimoto M~~, Kitaoka M~~, Igarashi K, Ohtsubo K~~, Nakai H ~~FEBS Lett~~. ~~2013 Nov 1;587(21):3556-61~~. ~~doi~~: 10.~~1016/j~~.~~febslet.2013.09.014. Epub, 2013 Sep 19~~. PMID:~~24055472~~<ref>PMID:~~24055472~~</ref>	+	Crystal structure and substrate recognition of cellobionic acid phosphorylase playing a key role in oxidative cellulose degradation by microbes.,Nam YW, Nihira T, Arakawa T, Saito Y, Kitaoka M, Nakai H, Fushinobu S J Biol Chem. 2015 Jun 3. pii: jbc.M115.664664. PMID:26041776<ref>PMID:26041776</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

OCA at 13:57, 10 June 2015

OCA — Wed, 10 Jun 2015 13:57:38 GMT

←Older revision		Revision as of 13:57, 10 June 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Cellobionic acid phosphorylase - cellobionic acid complex==
		+	<StructureSection load='4zlf' size='340' side='right' caption='[[4zlf]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4zlf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZLF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZLF FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CEZ:4-O-BETA-D-GLUCOPYRANOSYL-D-GLUCONIC+ACID'>CEZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4zle\|4zle]], [[4zlg\|4zlg]], [[4zli\|4zli]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellobionic_acid_phosphorylase Cellobionic acid phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.321 2.4.1.321] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zlf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4zlf RCSB], [http://www.ebi.ac.uk/pdbsum/4zlf PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A novel phosphorylase was characterized as new member of glycoside hydrolase family 94 from the cellulolytic bacterium Xanthomonas campestris and the fungus Neurospora crassa. The enzyme catalyzed reversible phosphorolysis of cellobionic acid. We propose 4-O-beta-D-glucopyranosyl-D-gluconic acid: phosphate alpha-D-glucosyltransferase as the systematic name and cellobionic acid phosphorylase as the short names for the novel enzyme. Several cellulolytic fungi of the phylum Ascomycota also possess homologous proteins. We, therefore, suggest that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively.

-	~~The entry 4zlf is ON HOLD until May 01 2017~~	+	Discovery of cellobionic acid phosphorylase in cellulolytic bacteria and fungi.,Nihira T, Saito Y, Nishimoto M, Kitaoka M, Igarashi K, Ohtsubo K, Nakai H FEBS Lett. 2013 Nov 1;587(21):3556-61. doi: 10.1016/j.febslet.2013.09.014. Epub, 2013 Sep 19. PMID:24055472<ref>PMID:24055472</ref>

-	~~Authors: Nam~~, Y.~~W., Arakawa, T., Fushinobu,~~ S.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~:	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cellobionic acid phosphorylase]]
		+	[[Category: Arakawa, T]]
	[[Category: Fushinobu, S]]		[[Category: Fushinobu, S]]
-	[[Category: Nam, Y.W]]	+	[[Category: Nam, Y W]]
-	[[Category: ~~Arakawa, T~~]]	+	[[Category: Glycoside hydrolase family 94]]
		+	[[Category: Intracellular enzyme]]
		+	[[Category: Oxidative cellulose degradation system]]
		+	[[Category: Transferase]]

OCA at 10:52, 20 May 2015

OCA — Wed, 20 May 2015 10:52:10 GMT

←Older revision		Revision as of 10:52, 20 May 2015
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4zlf is ON HOLD	+	The entry 4zlf is ON HOLD until May 01 2017

	Authors: Nam, Y.W., Arakawa, T., Fushinobu, S.		Authors: Nam, Y.W., Arakawa, T., Fushinobu, S.

OCA: Protected "4zlf" [edit=sysop:move=sysop]

OCA — Wed, 13 May 2015 11:05:23 GMT

Protected "4zlf" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:05, 13 May 2015

OCA: New page: '''Unreleased structure''' The entry 4zlf is ON HOLD Authors: Nam, Y.W., Arakawa, T., Fushinobu, S. Description: Category: Unreleased Structures Category: Fushinobu, S [[Categ...

OCA — Wed, 13 May 2015 11:05:21 GMT

New page: '''Unreleased structure''' The entry 4zlf is ON HOLD Authors: Nam, Y.W., Arakawa, T., Fushinobu, S. Description: Category: Unreleased Structures Category: Fushinobu, S [[Categ...

New page

'''Unreleased structure'''

The entry 4zlf is ON HOLD

Authors: Nam, Y.W., Arakawa, T., Fushinobu, S.

Description:
[[Category: Unreleased Structures]]
[[Category: Fushinobu, S]]
[[Category: Nam, Y.W]]
[[Category: Arakawa, T]]